Phosphorus Uptake Research Articles

Pengzhenrongella phosphoraccumulans sp. nov., isolated from high Arctic glacial till, and emended description of the genus Pengzhenrongella.

A yellow pigmented, Gram-stain-positive, motile, facultatively anaerobic and irregular rod-shaped bacteria (strain M0-14T) was isolated from a till sample collected from the foreland of a high Arctic glacier near the settlement of Ny-Ålesund in the Svalbard Archipelago, Norway. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that M0-14T formed a lineage within the family Cellulomonadaceae, suborder Micrococcineae. M0-14T represented a novel member of the genus Pengzhenrongella and had highest 16S rRNA gene sequence similarity to Pengzhenrongella sicca LRZ-2T (97.3 %). Growth occurred at 4-25 °C (optimum 4-18 °C), at pH 6.0-9.0 (optimum pH 7.0), and in the presence of 0-5 % (w/v) NaCl. The predominant menaquinone was MK-9(H4) and the major fatty acids were anteiso-C15 : 0, C16 : 0 and summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c). The major polar lipids were phosphatidylglycerol, phosphatidylinositol mannosides, phosphatidylinositol, one undefined phospholipid and five undefined phosphoglycolipids. The cell-wall diamino acid was l-ornithine whereas rhamnose and mannose were the cell-wall sugars. Polyphosphate particles were found inside the cells of M0-14T. Polyphosphate kinase and polyphosphate-dependent glucokinase genes were detected during genomic sequencing of M0-14. In addition, the complete pstSCAB gene cluster and phnCDE synthesis genes, which are important for the uptake and transport of phosphorus in cells, were annotated in the genomic data. According to the genomic data, M0-14T has a metabolic pathway related to phosphorus accumulation. The DNA G+C content of the genomic DNA was 70.8 %. On the basis of its phylogenetic relationship, phenotypic properties and chemotaxonomic distinctiveness, strain M0-14T represents a novel species of the genus Pengzhenrongella, for which the name Pengzhenrongella phosphoraccumulans sp. nov. is proposed. The type strain is M0-14T (= CCTCC AB 2012967T = NRRL B-59105T).

Studies on Nutrient uptake, Nutrient use Efficiency and Water Productivity of Drip Irrigated Ratoon Sugarcane as Influenced by Water Soluble Fertilizers

A field experiment was conducted at Zonal Agricultural Research Station, V.C. Farm, Mandya during 2020-21 to study the Nutrient content and Uptake of Drip irrigated Ratoon Sugarcane as influenced by water soluble fertilizers. The experimental site was red sandy loam soil with neutral pH, normal electrical conductivity, medium organic carbon, low available nitrogen, medium phosphorus and potassium content. The experiment was laid out in Randomized Complete Block Design with three replications comprising seven treatments with VCF-0517 sugarcane variety. The results revealed that application of 150 per cent RDF through WSF (325.9 kg ha-1 N, 46.5 kg ha-1 P2O5, 338.4 K2O kg ha-1) recorded significantly higher nitrogen, phosphorus and potassium uptake compared to 100 per cent RDF through WSF (287.3 kg ha-1 N, 37.5 kg ha-1 P2O5, 282.6 kg ha-1 K2O) and 75 per cent RDF through WSF (269.9 kg ha-1 N, 33.4 kg ha-1 P2O5, 253.6 kg ha-1 K2O). Application of WSF at 75 per cent RDF resulted in greater NUE in case of nitrogen, phosphorus and potassium (348.3 kg kg-1, 870.7 kg kg-1 and 696.5 kg kg-1 respectively) when compared with other fertigation levels and conventional fertilizer application.

ZIF-8-derived N-doped porous carbon electrodes enabled with in situ embedded ZrO2 nanoparticles for phosphorus removal

Excessive phosphorus discharges are not favorable to the normal functioning of water ecosystems. Although some treatments have been implemented to control phosphorus concentrations in sewage, several challenges remain for them. Capacitive deionization technology (CDI) possesses excellent potential for phosphorus removal, and the electrode material is the key to CDI. Herein, a novel ZIF-8-derived N-doped porous carbon electrode enabled with in situ embedded ZrO2 nanoparticles (ZNPC) was fabricated through a simple in-situ growth approach. The experimental results demonstrate that ZrO2 embedded in ZNPC can improve the electrochemical performance and provide suitable affinity sites for phosphorus, thus enhancing the electrode performance in capturing phosphorus. Isothermal and kinetic models were adopted to investigate the phosphorus uptake process. Mechanism analysis reveals that the phosphorus removal behavior is associated with the electric field, ligand exchange, and electrostatic attraction. This study provides a promising approach for facile constructing Zr-based N-doped porous carbon and expands its potential utility for phosphorus removal from aqueous solutions.

Overexpression of LbPT7 Promotes Phosphorus Uptake by Plants and Affects Phosphorus Uptake by Arbuscular Mycorrhizas under High Phosphorus Condition

Overexpression of LbPT7 Promotes Phosphorus Uptake by Plants and Affects Phosphorus Uptake by Arbuscular Mycorrhizas under High Phosphorus Condition

Intensive Microalgal Cultivation and Tertiary Phosphorus Recovery from Wastewaters via the EcoRecover Process.

Mixed community microalgal wastewater treatment technologies have the potential to advance the limits of technology for biological nutrient recovery while producing a renewable carbon feedstock, but a deeper understanding of their performance is required for system optimization and control. In this study, we characterized the performance of a 568 m3·day-1 Clearas EcoRecover system for tertiary phosphorus removal (and recovery as biomass) at an operating water resource recovery facility (WRRF). The process consists of a (dark) mix tank, photobioreactors (PBRs), and a membrane tank with ultrafiltration membranes for the separation of hydraulic and solids residence times. Through continuous online monitoring, long-term on-site monitoring, and on-site batch experiments, we demonstrate (i) the importance of carbohydrate storage in PBRs to support phosphorus uptake under dark conditions in the mix tank and (ii) the potential for polyphosphate accumulation in the mixed algal communities. Over a 3-month winter period with limited outside influences (e.g., no major upstream process changes), the effluent total phosphorus (TP) concentration was 0.03 ± 0.03 mg-P·L-1 (0.01 ± 0.02 mg-P·L-1 orthophosphate). Core microbial community taxa included Chlorella spp., Scenedesmus spp., and Monoraphidium spp., and key indicators of stable performance included near-neutral pH, sufficient alkalinity, and a diel rhythm in dissolved oxygen.

OsPHR2-mediated recruitment of Pseudomonadaceae enhances rice phosphorus uptake

Plants can shape their root microbiome to promote growth and nutrient uptake. PHOSPHATE STARVATION RESPONSE 2 (OsPHR2) is a central regulator of phosphate signaling in rice, but whether OsPHR2 can shape the root microbiome to promote phosphorus uptake is unclear. Here, we investigate the role of OsPHR2 in recruiting microbiota for phosphorus uptake using high-throughput sequencing and metabolite analysis. OsPHR2-overexpressing (OsPHR2 OE) rice showed 69.8% greater shoot P uptake in natural soil compared with sterilized soil under high-phosphorus (HP) conditions, but there was only a 54.8% increase in the wild-type (WT). The abundance of the family Pseudomonadaceae was significantly enriched in OsPHR2 OE roots relative to those of WT rice. Compared with the WT, OsPHR2 OE rice had a relatively higher abundance of succinic acid and methylmalonic acid, which could stimulate the growth of Pseudomonas sp. (P6). After inoculation with P6, phosphorus uptake in WT and OsPHR2 OE rice was higher than that in uninoculated rice under low-phosphorus (LP) conditions. Taken together, our results suggest that OsPHR2 can increase phosphorus use in rice through root exudate-mediated recruitment of Pseudomonas. This finding reveals a cooperative contribution of the OsPHR2-modulated root microbiome, which is important for improving phosphorus use in agriculture.

Analysis of cultivar variability in arbuscular mycorrhizal responsiveness in soybean varieties

ABSTRACT In modern agricultural systems, high crop yields depend on the high input of orthophosphate-based fertilizers. Owing to the looming scarcity of rock phosphate in the near future, utilization of arbuscular mycorrhizal (AM) symbiosis for the efficient uptake of phosphorus is a necessary approach to maintain crop yield. Plant responsiveness to AM fungi is assessed based on the difference in biomass between plants with and without AM fungal inoculation. Soybean is a plant which establishes both mycorrhizal and rhizobial symbiosis. Therefore, it is difficult to eliminate the symbiotic effects of rhizobial nitrogen fixation during long-term cultivation until soybean harvest. In this study, Bradyrhizobium diazoefficiens USDA110 was inoculated at the same time as soybean seeds were sown, and soybean was grown under Rhizophagus irregularis inoculated and non-inoculated conditions to verify whether the mycorrhizal inoculation effect was observed at the yield level. In 2013, two soybean cultivars Tachinagaha and Williams 82, were grown at five levels of phosphate availability (0, 5, 10, 20, and 40 kg P 10a−1) in an open greenhouse in Tsukuba, Japan. Among the several growth indices analyzed, seed weight per plant was selected as a reliable indicator of plant response. Mycorrhizal inoculation showed a positive effect on soybean under 20 kg P 10a−1 phosphorus fertilization for Williams 82, while Tachinagaha showed almost no effect. This trend was replicated in 2014 and 2016, indicating that Tachinagaha has a lower AM responsiveness than Williams 82.

The core phoD-harboring bacteria promote wheat phosphorus uptake by enhancing alkaline phosphatase activity under long-term fertilization

The core phoD-harboring bacteria promote wheat phosphorus uptake by enhancing alkaline phosphatase activity under long-term fertilization

Exogenous application of bio-stimulants and growth retardants improve nutrient absorption and fiber quality in upland cotton

BackgroundNatural and synthetic plant growth regulators are essential for plant health, likewise these regulators also play a role in increasing organic production productivity and improving quality and yield stability. In the present study, we have evaluated the effects of foliar applied plant growth regulators, i.e., moringa leaf extract (MLE) and mepiquat chloride (MC) alone and in combination MC and MLE on the conventional cotton cultivar (CIM 573) and transgenic one (CIM 598). The growth regulators were applied at the start of bloom, 45 and 90 days after blooming.ResultsThe application of MC and MLE at 90 days after blooming significantly improved the relative growth rate, net assimilation rate, the number of bolls per plant, and seed cotton yield. Likewise, the combined application of MLE and MC at 90 days after blooming significantly boosted the nitrogen uptake in locules, as well as the phosphorus and potassium uptake in the leaves of both cotton cultivars. The application of MLE alone has considerably improved the nitrogen uptake in leaves, and phosphorus and potassium contents in locules of Bt and conventional cotton cultivars. Similarly, Bt cotton treated with MLE at 90 days after blooming produced significantly higher ginning out turn and oil contents. Treatment in combination (MLE + MC) at 90 days after blooming produced considerably higher micronaire value, fiber strength, and staple length in conventional cultivar.ConclusionThe natural growth enhancer, MLE is a rich source of minerals and zeatin, improving the nutrient absorption and quality of cotton fiber in both conventional and Bt cotton cultivars.

Response of root growth parameters and nutrient uptake of cowpea (<i>Vigna unguculata L.</i>)to rates of organic manure

This study was carried out to investigate the effect of different organic manure sources on Root growth parameters and nutrient uptake of cowpeas (Vigna unguiculata L.). The experiment was conducted at the Teaching and Research Farm of the Faculty of Agriculture, Imo State University, Owerri. The experiment was laid out in a Randomized Complete Block Design (RCBD), with five treatments replicated four times. The treatments are 0 ton T1 (Control), T2 (10tons and pig manure), T3 (15tons of pig manure), T4 (10tons of poultry manure), and T5 (15 tons of pig manure). From the result of the experiment, the application of poultry manure significantly improved, the number of roots, root length, root dry matter, phosphorus, and potassium uptake at various growth stages of data collection. The result also showed that pig manure significantly improved the percentage of emergence, plant height, number of leaves, Nitrogen uptake, and high number of pods (9.75), seed weight (101.47g), and yield 924.16kg/ha). The cowpea responded significantly in both root growth parameters and nutrient uptake to pig manure and poultry manure at the rates used.

Enhancing phosphorus use efficiency and soil quality indicators in lowland paddy ecosystem through Azolla, rice straw, and NPKS fertilizers

PurposeThis study investigates the influence of incorporating Azolla, rice straw, and NPKS fertilizers on phosphorus use efficiency (PUE) and rice productivity in lowland paddy fields. Despite Azolla’s well-known role as a nitrogen-fixing aquatic fern in rice production, its specific impact on PUE remains unclear. The primary objective is to explore diverse treatment combinations to identify synergies that enhance both PUE and overall rice productivity.MethodsThe study was conducted at Mkula Irrigation Scheme in the Kilombero Valley, Tanzania; the field experiment employed a randomized complete block design with 13 treatments and three replications. Treatments comprised various combinations of Azolla, rice straw, and chemical fertilizers, incorporating 50% and 100% rates of nitrogen (N) applied with phosphorus (P), potassium (K), and sulfur (S).ResultsThe study reveals the substantial impact of Azolla application on total nitrogen, available phosphorus, and exchangeable potassium levels in the soil. Particularly noteworthy were treatment combinations involving Azolla, rice straw, and reduced rates of synthetic nitrogen, along with specific P, K, and S applications, which exhibited the highest phosphorus uptake and PUE. Specifically, combining rice straw and Azolla with reduced N rates, alongside 30 kg P ha−1 + 30 kg K ha−1 + 20 kg S ha−1, resulted in the highest phosphorus uptake (73.57 kg/ha) and PUE (46.24%).ConclusionIntegrated nutrient management, incorporating rice straw and Azolla alongside synthetic fertilizers, demonstrates synergistic effects on phosphorus uptake and efficiency while maintaining soil quality. The study underscores the potential of such integrated strategies to optimize PUE and contribute to sustainable rice production in lowland paddy fields.

Effects of Short-Term Nitrogen Additions on Biomass and Soil Phytochemical Cycling in Alpine Grasslands of Tianshan, China.

The nitrogen deposition process, as an important phenomenon of global climate change and an important link in the nitrogen cycle, has had serious and far-reaching impacts on grassland ecosystems. This study aimed to investigate the survival adaptation strategies of plants of different functional groups under nitrogen deposition, and the study identified the following outcomes of differences in biomass changes by conducting in situ simulated nitrogen deposition experiments while integrating plant nutrient contents and soil physicochemical properties: (1) nitrogen addition enhanced the aboveground biomass of grassland communities, in which Poaceae were significantly affected by nitrogen addition. Additionally, nitrogen addition significantly influenced plant total nitrogen and total phosphorus; (2) nitrogen addition improved the plant growth environment, alleviated plant nitrogen limitation, and promoted plant phosphorus uptake; and (3) there was variability in the biomass responses of different functional groups to nitrogen addition. The level of nitrogen addition was the primary factor affecting differences in biomass changes, while nitrogen addition frequency was an important factor affecting changes in plant community structure.

Biochemical mechanisms underlying iron plaque-mediated phosphorus accumulation and uptake in rice roots

The iron oxyhydroxides of iron plaque on the surface of rice root are crucial for the uptake of nutrition elements, especially phosphorus (P), but the effects of iron oxyhydroxides of iron plaque on the accumulation and uptake of P remain largely unknown. In this study, we investigated the regulatory mechanism of iron plaque on P uptake in rice via hydroponics of whole plant and simulation of iron oxyhydroxides-coated suspension cells in rice. The hydroponic experiment results showed that the presence of iron plaque increased the P content in rice shoots. The simulation experiment results further confirmed that after iron plaque coating, the P contents in the whole cell and on the cell wall were significantly increased from 5.16 mg/g and 2.73 mg/g to 8.85 mg/g and 5.27 mg/g, respectively. In addition, our data also showed that iron plaque coating led to an increase in cell surface potentials from −380 ± 40 mV to −200 ± 30 mV, thus promoting the adsorption of more P. Taken together, this study demonstrated that the iron plaque coating increased the surface potential of the cells, thus enhancing cellular P enrichment, eventually promoting P efficient adsorption in rice. Deciphering these regulatory mechanisms provide an insight into P biogeochemical cycling at the soil-plant interface and offer theoretical basis and practical references for the improvement of P bioavailability in rice production.

Phosphorus Uptake Model of Oil Palm Seedlings in the Main Nursery

Oil palm is generally cultivated on tropical soils that have low levels of chemical fertility and various physical fertility. Environmental factors, genetics, and cultivation techniques generally influence oil palm productivity. This study aimed to obtain a model of oil palm growth and phosphorus (P) uptake in the main nursery. The oil palm growth model was carried out through literature studies and field trials. The trial was conducted at Leuwikopo Trial Farm (Bogor Agricultural Institute) IPB University from May 2021-January 2022. The experiment was designed using a non-factorial randomized block design with five replications. The experiment consisted of one treatment with five levels of P fertilization: P0 = no fertilizer; P1 = 50% standard fertilization; P2 = 100% standard fertilization; P3 = 150% standard fertilization; and P4 = 200% standard fertilization. The fertilizer dose of 100% using the standard fertilization for oil palm of the Damimas variety was 28 g P seedling-1. This study concluded that the model of oil palm nutrient P uptake for the main nursery was able to simulate oil palm nutrient uptake as shown by actual measurements (observations in the field). The dry weight simulation results fell within the range of standard deviation values for average measurements in the field. The nutrient uptake simulation model is thus a valid tool for planning the optimal fertilization of oil palm seedlings in the main nursery.

Genetic Variability Analysis for Yield and Its Attributing Traits in Rice (Oryza Sativa L.) under Phosphorus-sufficient and Deficient Field Conditions

Aim: The present study aims to identify phosphorus deficiency tolerant rice genotypes under both phosphorus-sufficient and deficient conditions. Materials and Methods: Two kinds of fields (high and low phosphorus content) were selected based on their soil phosphorus availability. Hundred diverse rice germplasm was collected and planted in an augmented design along with four local checks namely, Swarna sub-1, MTU 1153, MTU 7029 and Uttar Sona. The data was recorded for 14 traits and subjected to different types of variability analysis. Place and Season of Study: The experiment took place at instructional Farm of Uttar Banga Krishi Vishwavidyalaya, Cooch Behar, West Bengal, India during the Kharif season of 2021. Results: The variability analysis revealed that phosphorus uptake showed a high genotypic coefficient of variation (GCV) under phosphorus-deficient condition and high phenotypic coefficient of variation (PCV) under both conditions. Grain yield showed high GCV and PCV under both the phosphorus conditions. Heritability was moderate whereas high Genetic advance as percentage of mean was observed under both the conditions for both phosphorus uptake and grain yield. Strong positive correlations were observed between days to flowering and various yield-related traits under both the conditions. Positive correlation was observed between phosphorus uptake and grain yield under phosphorus-sufficient condition, but a weaker correlation under phosphorus-deficient condition.

Drought accentuates the role of mycorrhiza in phosphorus uptake, part II – The intraradical enzymatic response

Edaphic drought reduces phosphorus (P) diffusivity in soils and arbuscular mycorrhizal fungi (AMF) can compensate for this. We recently showed, along a high-resolution substrate moisture gradient, that AMF effectively deliver P to plants under drought from areas beyond the reach of roots. Here, we investigated how edaphic drought affected the active sites of P exchange between AMF and plants inside the roots using different histochemical stains.Inoculation of dwarf tomato plants (cv. MicroTom) with AMF (Rhizophagus irregularis) slightly increased water retention (decreased the water potential) in substrates, decreased root length, and increased shoot P concentrations compared to non-mycorrhizal plants. Despite their reduced root length, mycorrhizal tomato plants showed a surprisingly congruent physiological drought stress response with their non-mycorrhizal counterparts. However, the activity of alkaline phosphatase (ALP) detected in intraradical fungal structures decreased significantly as soil and xylem water potentials decreased. The decline of ALP activity with increasing drought intensity was accompanied by a reduction of elevated P concentration benefits in shoots of mycorrhizal plants compared to non-mycorrhizal plants.Therefore, we conclude that edaphic drought can restrict the nutritional advantages that plants receive from AMF at the root-fungal interface. This is one of the direct consequences of drought.

Factors affecting phosphorus uptake/dissolution during slaking and causticizing

Hydroxide is regenerated in the recovery cycle of kraft pulp mills by the addition of lime (CaO) to green liquor. Phosphate in green liquor can react with the lime during slaking/causticizing. Total titratable alkali (TTA), sulfidity, the concentration of phosphate in the green liquor, temperature, and the liming ratio were all vari-ables explored in this work to determine their influence on phosphorus uptake and dissolution. Experiments were also run in which the lime was slaked before being added to the green liquor to separate reactions with phosphate during slaking and reactions that occur during causticizing. Both reburnt lime and technical grade CaO were used. The experiment results indicate that phosphorus primarily reacts with slaked lime (Ca(OH)2), and that the final con-centration of phosphate in the white liquor at the end of slaking and causticizing is nearly independent of the initial concentration of phosphorus and only mildly dependent on the carbonate concentration in the green liquor. There do appear to be differences in the rate at which phosphate reacts with reburnt lime and technical grade CaO, though the reason for this was not determined.

Influence of rice straw biochar addition on soil traits and nutrient uptake of Artichoke (Cynara cardunculus)grown in Mercury-contaminatedsoils

The present pot experiment was therefore conducted to investigate the effect of biochar application on the selected properties of mercury polluted soils and uptake of Artichoke (Cynara cardunculus) grown in polluted soils.Biochar obtained from rice straw was added at various rates (0, 5, 10 t/ha) on the soil artificially contaminated with mercury at a different concentration (0, 10, 20 ppm). The research indicated a significant (P < 0.01) improvement in electrical conductivity, pH, available phosphorus, total nitrogen, exchangeable bases, cation exchange capacity and organic carbon owing to biochar addition.Additionally, uptake of potassium, phosphorus and nitrogen by Artichoke (Cynara cardunculus) was significantly enhanced by biochar application. A significant decrease in uptake of mercury owing to biochar application was also noticed in heavily mercury-contaminated soil (20 ppm). Hence, biochar application is mostvital to improve soil quality and fertility, improve nutrients uptake, amend Hg contaminated soil and decrease the quantity of carbon formedowing to the biomassburning.

ACC deaminase producing bacteria alleviate the polyethylene glycol induced drought stress in black gram (Vigna mungo L.) by enhancing nutrient uptake and soil respiration activity

The present study aimed to exploit ACC deaminase (ACCD) producing drought-tolerant bacteria to mitigate drought stress (PEG stress) and simultaneous enhancement of the essential nutrients (N and P), relative water content (RWC), and chlorophyll pigment. In this study, 80 previously isolated ACCD-producing bacteria were screened for drought tolerance, and the results showed that 13.75 % (11isolates) were tolerant to up to -0.73 MPa. Further, screening of these drought tolerant ACCD producers revealed that the 11 isolates produced siderophore (96.82–99.67 %), indole-3-acetic acid like substances (15.15–70.55 µg/mL), and phosphate solubilization (39.33–142.67 µg/mL). Furthermore, pot studies (2000 ppm PEG stress) revealed an enhanced root (40.97–174.24 %) and shoot length (14.06–194.86 %) in inoculated plants compared to the control plants. In addition, inoculated plants also enhanced the uptake of nitrogen (35.10–42.61 mg/g) and phosphorus (2.67–3.68 mg/g), relative water content (55.26–83.33 %), chlorophyll (15.83–47.25 mg/g) and ACCD production in the soils (2.03–5.51 µmol/mL). The inoculated bacteria also enhanced the soil respiration activity such as dehydrogenase enzyme (65.31–144.48 µg/g), fluorescein diacetate hydrolysis (19.27–55.25 µg/g), and alkaline phosphatase (611.53–1011.53 µg/g). In conclusion, IPTS 7 (Rhodococcus kroppenstedtii) and CWN 5 (Bacillus altitudinis) can be used to mitigate drought stress and mobilize essential nutrients (N and P).

Perfluorooctanesulfonic Acid Alters the Plant's Phosphate Transport Gene Network and Exhibits Antagonistic Effects on the Phosphate Uptake.

Per- and polyfluoroalkyl substances (PFASs), with significant health risks to humans and wildlife, bioaccumulate in plants. However, the mechanisms underlying plant uptake remain poorly understood. This study deployed transcriptomic analysis coupled with genetic and physiological studies using Arabidopsis to investigate how plants respond to perfluorooctanesulfonic acid (PFOS), a long-chain PFAS. We observed increased expressions of genes involved in plant uptake and transport of phosphorus, an essential plant nutrient, suggesting intertwined uptake and transport processes of phosphorus and PFOS. Furthermore, PFOS-altered response differed from the phosphorus deficiency response, disrupting phosphorus metabolism to increase phosphate transporter (PHT) transcript. Interestingly, pht1;2 and pht1;8 mutants showed reduced sensitivity to PFOS compared to that of the wild type, implying an important role of phosphate transporters in PFOS sensing. Furthermore, PFOS accumulated less in the shoots of the pht1;8 mutant, indicating the involvement of PHT1;8 protein in translocating PFOS from roots to shoots. Supplementing phosphate improved plant's tolerance to PFOS and reduced PFOS uptake, suggesting that manipulating the phosphate source in PFOS-contaminated soils may be a promising strategy for minimizing PFOS uptake by edible crops or promoting PFOS uptake during phytoremediation. This study highlighted the critical role of phosphate sensing and transport system in the uptake and translocation of PFOS in plants.