Phosphorus Absorption Research Articles

Effects of Biochar and Organic Acid Addition on Phosphorus State and Yield of Cotton Field Under Different Phosphate Fertilizer Application Rates

The purpose of this study is to investigate the effect of biochar and organic acid on soil phosphorus effectiveness, plant accumulation phosphorus absorption, yield composition, and phosphorus equilibrium under different phosphorus fertilizer dosage conditions. Using the field test method, different phosphate fertilizer dosage treatments (0, 50, 100, 150 kg P2O5/ha) were set, and a control group, biochar (BC), and organic acid (OA) addition treatment were set up on this basis. During the experiment, the soil fast-acting phosphorus, accumulated phosphorus absorption, and cottonseed yield of cotton were measured, and the utilization of phosphorus fertilizer and phosphorus surplus were calculated. The results showed that the addition of biochar and organic acid significantly improved the soil phosphorus content, plant accumulation phosphorus absorption, cottonseed yield, and phosphate fertilizer utilization. In particular, in terms of plant accumulation of phosphorus absorption, cottonseed production and phosphate fertilizer utilization, biochar was better than organic acids, increasing by 10.2%, 0.29%, and 0.21%, respectively. Under the conditions of phosphorus fertilizer loss, the addition of biochar can effectively improve the effectiveness of phosphorus in soil and regulate the absorption and distribution of phosphorus in cotton, thus promoting the yield of cottonseed. In summary, the addition of biochar has important application potential in phosphorus management in cotton fields, which can provide a scientific basis for the sustainable development of cotton fields in Xinjiang. The results provide a reference for the rational use and management of phosphate fertilizer in cotton fields and promote the sustainable development of agriculture.

Isolation and characterization of phosphate solubilizing bacteria from the rhizosphere of lentil (Lens culinaris M.) collected from Hagere Mariam district, Central Ethiopia.

Phosphorus plays a crucial role in regulating many of the plant's metabolic activities by enhancing physiological functions and stimulating biological activities such as nodulation, nitrogen fixation, and nutrient uptake in the soil rhizosphere environment. Inoculants of phosphorus solubilizing bacteria serve as an eco-friendly alternative technology that positively influences both soil sustainability and plant growth. The majority of North Shewa highland areas are characterized by low available phosphorus, primarily acidic, and exhibit strong phosphorus absorption. The objective of this study was to isolate and identify phosphorus solubilizing bacteria from the rhizosphere of lentils and characterize their phosphate solubilizing activity. The cultural, biochemical, physiological microbial analysis was conducted in the microbiology laboratory, department of biology. Pikovskaya's medium was utilized for the isolation, screening, and maintenance of phosphate solubilizing bacteria. Phosphate Solubilizing Bacteria were isolated using tri-calcium phosphate as the sole source of phosphorus in indicator plates. Fifteen phosphate solubilizing bacteria were isolated from lentil rhizosphere soil samples, among which six were the most efficient phosphate solubilizers designated as PSBYE, PSBYR, PSBYM, PSBYL, PSBW, and PSBSW. All isolates notably solubilized tri-calcium phosphate compared to the uninoculated control. The highest phosphorous solubilization was observed from the isolate PSBYL, with a value of 10.61mg/50ml, followed by PSBW with a value of 9.08 mg/50ml. The decrease in pH value correlated with the levels of tri-phosphate solubilization in the PVK broth by the PSB isolates. The pH dropped to 4.64 from the initial pH of 7.2 when grown in the broth, which suggests that the production of organic acids is likely the primary mechanism for phosphate solubilization.

Approximation of the Absorption Spectrum of Indium Phosphide in the Context of Simulation of the Process of Sensitivity Enhancement

Examines the fundamental rights of Phosphide of India, legitimized by Tellur and, in the last resort, compensated for. Data on the composition of four high-quality images of the InP: Cu photoelectronic spectrum are presented in the individual layers. The work is characterized by the semi-empiric approach to photoconduction of InP: Cu oxide films. It is concluded that the photoresistance Iph (α(ћω)) was analytically approximated as the function of the experimental-complete spectral distribution of the coefficiency phosphorus india. It is proposed to use five approximating functions with the aim of analyzing the coefficient of absorption of Indian phosphorus α(ћω). Completed 5 locations with different signs of median isolation. On the basis of analytical amplitudes, the complete analytical amplitude Iph(α(ћω)) is modelled. Analogously, five conclusions were drawn that indicate a sign of median isolation. Five non-stationary measures of IF photometry have been taken (as two functions: co-efficiency of exposure, as photo energy functions, and time-consuming observations) in normal situations. The answer to the question is the most mathematical and physical solution of the proximate function α(ћω). Obviously, it shows that this the degree of variance is optimal for its implementation (inclusion of this degree of variance in the structure Iph = f(α) and α = f(ћω)) of the complete analytical description of the process photoconductivity. It should be noted that subsequent research may be based on the establishment of physical bases of photoconductivity in the short wave of fundamental transfers of phosphorus from India, as well as research into the properties of air on the high InP: Cu layer, with its stability and stability.

Intercropping of tobacco and maize at seedling stage promotes crop growth through manipulating rhizosphere microenvironment.

Changes in the rhizosphere microbiome and metabolites resulting from crop intercropping can significantly enhance crop growth. While there has been an increasing number of studies on various crop combinations, research on the intercropping of tobacco and maize at seedling stage remains limited. This study is the first to explore rhizosphere effects of intercropping between tobacco and maize seedling stages, we analyzed the nitrogen, phosphorus and potassium nutrients in the soil, and revealed the important effects on soil microbial community composition and metabolite profiles, thereby regulating crop growth and improving soil balance. Compared with mono-cropping, intercropping increased the biomass of the two crops and promoted the nutrient absorption of nitrogen, phosphorus and potassium. Under intercropping conditions, the activities of sucrase, catalase and nitrate reductase in tobacco rhizosphere soil and the content of available potassium, the activities of nitrate reductase and acid phosphatase in maize rhizosphere soil were significantly increasing. Rhizosphere soil bacterial and fungal communities such as Sphingomonas, Massilia, Humicola and Penicillium respond differently to crop planting patterns, and soil dominant microbial communities are regulated by environmental factors such as pH, Organic Matter, Available Potassium, Nitrate Reductase, and Urease Enzyme. Network analysis showed that soil microbial communities were more complex after intercropping, and the reciprocal relationship between bacteria and fungi was enhanced. The difference of metabolites in soil between intercropping and monocropping system was mainly concentrated in galactose metabolism, starch and sucrose metabolism pathway, and the content of carbohydrate metabolites was significantly higher than that of monocropping soil. Key metabolites such as D-Sucrose, D-Fructose-6-Phosphate, D-Glucose-1-Phosphatel significantly influence the composition of dominant microbial communities such as Sphingomonas and Penicillium. This study explained the effects of intercropping between flue-cured tobacco and maize on the content of soil metabolites and soil microbial composition in rhizosphere soil, and deepened the understanding that intercropping system can improve the growth of flue-cured crops seedlings through rhizosphere effects.

Reclaiming Calcicoles: New Insights into Lime Lovers

Calcicoles are never new to the evolutionary life forms in the ecological world. The ramifications of environmental extremes always facilitate the emergence of the functional and structural integrity of plants and calcicoles, which have been exuding through this. The word “calcicole” finds its roots in the writings of Nathaniel Colgan, “The Orchids of Country Dublin,” published in August 1895 in “The Irish Naturalist.” Calcicoles are likely to flourish under calcium-rich soil having a pH of more than five, which otherwise hinders the growth of plant species, causing detrimental effects on their physiology. They are often regarded as lime lovers or acidofuges and are classified based on Ca2+ stress resistance, soluble and insoluble Ca2+ and Mg2+, pH conditions of soil, occurrence, and habitat. There are various variables used to evaluate the calcicoles described in this review, such as the Index of calcifugy, Ellenberg’s indicator values, and Landolt indicator values to predict the influence of environmental factors in determining flora along with changes in vegetation in a particular area considering the ecophysiological or morphological features. Calcicoles have specific in-built mechanisms to cope with stress conditions by producing phytosiderophores (PS) and by involving ecto and ericoid mycorrhizal associations for the absorption of iron and phosphorus, and high control over cytosolic Ca2+ concentrations. Calcicoles are markedly distributed in various plant families like Asteraceae, Brassicaceae, Amaranthaceae, Caryophyllaceae, Ericaceae, Primulaceae, Fabaceae, Gentianaceae, Rubiaceae, Plantaginaceae, Poaceae, Saxifragaceae, etc. The current review focuses on the ecological perspectives, special adaptive features, and phytopharmacological aspects of calcicoles.

Halotolerant phosphate solubilizing bacteria isolated from arid area in Tunisia improve P status and photosynthetic activity of cultivated barley under P shortage

Forty-seven (47) bacterial strains were isolated from soil of Gabes (an arid region in southern Tunisia) and were screened for their ability to produce Indole-3-Acetic Acid (IAA) and to solubilize phosphate (P). The characterization and molecular identification of the most successful P-solubilizing bacteria (PSB) were then carried out. When grown on suitable artificial media, the most salt-tolerant strains also showed the highest P solubilization capacity (up to 126.8 μg ml−1 of released phosphorus after 7 day incubation) and the strongest ability to produce IAA (up to 101.86 μg ml−1 after 3 day incubation). Overall, bacterial isolates displayed a different tolerance to varying pH, temperatures, and salinity. The molecular identification revealed that 11 strains belonged to three genera: Bacillus, Pseudomonas and Mesorhizobium. Inoculation of barley with P-solubilizing bacteria under tricalcium phosphate-induced P shortage significantly improved plant growth (biomass, shoot height, and root length) together with increasing total chlorophyll contents and photosynthetic activity. This was concomitant with (i) higher P uptake and translocation and (ii) increased phosphorus absorption and utilization efficiencies (PAE and PUE), which is indicative of a better plant P nutrition under P scarcity. Taken together, we provide strong arguments showing that bacteria native to extreme environments display PSB potential making them promising candidates to mitigate low Pi availability for crop plants.

PSXIII-21 Fiber digestibility and phosphorus absorption by crossbred heifers receiving different phosphates

Abstract The objective was to compare the potential of different phosphates in promoting improvement in fiber digestibility and phosphorus absorption by heifers. Crossbred weaned heifers [n = 5; body weight (BW) = 150 ± 14 kg] were used in a 5 × 5 Latin square design and offered diets harvested from processed forage seeds for 21-d periods (14-d adaptation and 7-d data collection). The treatments were 1) negative control (NC) without supplementary phosphorus; 2) dicalcium phosphate from igneous rock with sulfuric acidulation, neutralization with limestone, and the show physical form of micro granules (DPIRSALNG); 3) Dicalcium phosphate from igneous rock with sulfuric acidulation, neutralization with hydrated lime, and powder physical form (DPIRSAHLiNP); 4) Dicalcium phosphate from sedimentary rock with hydrochloric acidulation, neutralization with limestone, and the micro granulated show physical form (DPSRHCALNG); and 5) Dicalcium phosphate from the monocalcium phosphate and hydrated lime reaction with phosphorus concentration adjusted with phosphoric acid, and powder physical form (DPfMP+HLPAP). In each sample collection period two dry matter intake measurements, and two total feces collections were carried out for 24 h. Thus, the digestibility of dry matter (DMd), and the apparent absorption (aA) were calculated. Data were subjected to analysis of variance (P < 0.05), and when significant, a multiple comparison test was performed (SNK test). No treatment x animal x period interactions were observed (P ≥ 0.05) for either of the two variables under study. The greatest neutral detergent fiber (NDF) digestibilities were in the diets with DPIRSALNG and DPSRHCALNG, followed by DPIRSAHLiNP and DPfMP+HLPAP, which did not differ from each other, and the least was in NC, respectively 430, 390, 310, and 270 g/kg of DM (Table 1). Phosphorus apparent absorption (PaA) was greater (P < 0.05) in heifers that received DPIRSALNG (0.65) followed by DPSRHCALNG (0.61). DPIRSAHLiNP (0.52) and DPfMP+HLPAP (0.48) did not differ from each other (P > 0.05) and were greater (P < 0.05) than NC (0.21). Phosphorus supplementation resulted in greater NDF digestibility and greater phosphorus absorption regardless of phosphate sources. The sources DPIRSALNG and DPSRHCALNG were more efficient in terms of DMd and absorption.

The effects of polypropylene microplastics on the removal of nitrogen and phosphorus from water by Acorus calamus, Iris tectorum and functional microorganisms

Polypropylene microplastics (PP-MPs), an emerging pollutant, adversely affect the ability of aquatic plants to restore water bodies, thereby compromising the functionality and integrity of wetland ecosystems. This study examines the effects of microplastic stress on the nitrogen and phosphorus removal capacities of Acorus calamus and Iris tectorum, as well as on functional microorganisms within the aquatic system. The findings indicate that under PP-MP stress, the nitrogen and phosphorus absorption capabilities of both plants were diminished. Additionally, there was a significant reduction in the metabolic enzyme activities related to nitrogen and phosphorus in the plants, alongside a notable decrease in leaf nitrogen content. PP-MPs hinder the nutrient uptake of plants, affecting their growth and indirectly reducing their ability to utilize nitrogen and phosphorus. Specifically, in the 10 mg L−1 treatment group, A. calamus and I. tectorum showed reductions in leaf nitrogen content by 23.1% and 31.0%, respectively, and by 14.8% and 27.7% in the 200 mg L−1 treatment group. Furthermore, I. tectorum had higher leaf nitrogen levels than A. calamus. Using fluorescent tagging, the distribution of PP-MPs was traced in the roots, stems, and leaves of the plants, revealing significant growth impairment in both species. This included a considerable decline in photosynthetic pigment synthesis, enhanced oxidative stress responses, and increased lipid peroxidation in cell membranes. PP-MP exposure also significantly reduced the abundance of functional microorganisms involved in denitrification and phosphorus removal at the genus level in aquatic systems. Ecological function predictions revealed a notable decrease in nitrogen cycling functions such as nitrogen respiration and nitrite denitrification among water microorganisms in both treatment groups, with a higher ecological risk potential in the A. calamus treatment group. This study provides new insights into the potential stress mechanisms of PP-MPs on aquatic plants involved in water body remediation and their impacts on wetland ecosystems.

Phosphorus use efficiency in common bean genotypes

Phosphorus absorption in plants can vary depending on the genotypes, mineral levels, and environmental conditions. Thus, this study aimed to evaluate phosphorus use efficiency and the response capacity of five common bean genotypes in two cultivation cycles. A 2x5 factorial scheme was used with three replicates. Factor A consisted of using and not phosphorus fertilization, and factor B consisted of five common bean genotypes (BRS Expedito, Iraí, Pérola, Maronze, and Vermelho). The evaluated yield components were the number of pods per plant (NPP), the number of grains per plant (NGP), and the mass of 1000 grains (M1000G). The yield was calculated and extrapolated to kg ha-1. We also evaluated the phosphorus levels accumulated in the leaves and grains (mg and %) and estimated the use efficiency of using phosphorus in the grains. The data obtained were subjected to analysis of variance and compared using the Scott-Knott test at a 5% significance level. There was a significant interaction for all studied variables, indicating the interference of phosphorus fertilization in the behavior of the genotypes. The growing season influenced the genotypes' responses regarding phosphorus use. Yields increased with phosphorus fertilization and changed according to genotype. The phosphorus accumulated in the leaves and grains showed little divergence. The Iraí, Pérola, and Maronze genotypes stand out for their efficient use of phosphorus, presenting an increase above 50% when grown in soil with phosphorus fertilization. When fertilized, the Vermelho variety most accumulated phosphorus in the grains in the first season. In the second season, it showed a significant change in its response when using the phosphate fertilizer, indicating a change in behavior due to the environmental component.

Digestion and Absorption of Dietary Phosphorus in Fish

Digestion and Absorption of Dietary Phosphorus in Fish

Photosynthetic Response to Phosphorus Fertilization in Drought-Stressed Common Beech and Sessile Oak from Different Provenances.

Increasingly frequent and severe droughts pose significant threats to forest ecosystems, particularly affecting photosynthesis, a crucial physiological process for plant growth and biomass production. This study investigates the impact of phosphorus fertilization on the photosynthesis of common beech (Fagus sylvatica L.) and sessile oak (Quercus petraea (Matt.) Liebl.). In a common garden experiment, saplings originating from two provenances (wetter KA and drier SB provenances) were exposed to regular watering and drought in interaction with moderate and high phosphorus concentrations in the growing substrate. Results indicated that drought significantly reduced pre-dawn leaf water potential (ΨPD), net photosynthesis (Anet), stomatal conductance (gs) and photosynthetic performance index (PIabs) in both species. Phosphorus fertilization had a negative impact on Anet and PIabs, thus exacerbating the negative impact of drought on photosynthetic efficiency, potentially due to excessive phosphorus absorption by saplings. Provenance differences were notable, with the KA provenance showing better drought resilience. This research highlights the complexity of nutrient-drought interactions and underscores the need for cautious application of fertilization strategies in reforestation efforts under changing climatic conditions.

Changes in phosphorus-related performance attributes of dryland winter wheat cultivars released between the 1940s and 2010s in Shaanxi Province, China.

Water and nutrients are two main determinants of wheat yield, which are vital for maintaining high crop yields. In the present study, the effects of water and phosphate fertilization on wheat yield, photosynthetic parameters, water productivity and phosphate use efficiency were investigated. Five dryland wheat cultivars from the 1940s to the 2010s that are widely cultivated in Shaanxi Province, China, were used. Experiments were conducted from 2019 to 2022 using two irrigation levels (normal rainfall and no precipitation after the reviving stage) and two phosphorus application levels (0 and 100 kg ha-1). Compared with old cultivars ('Mazha'), the grain yield of modern cultivars ('Changhan 58') was 89.24% higher and was closely correlated with chlorophyll index, leaf area index, photosynthetic rate and tillers. With the replacement of cultivars, the phosphorus content, water potential and phosphatase activity of wheat leaves increased. Considering water-phosphorus interactions, the water use efficiency and phosphorus use efficiency of wheat showed a significant positive correlation. Our findings indicate that modern wheat cultivars are more responsive to phosphorus. Further analysis revealed that modern varieties have evolved two phosphorus absorption strategies in response to phosphorus deficiency - namely, the formation of a phosphorus supply source, which may result in larger numbers of green organs; and an increase in phosphorus sinks, which tended to activation and transport of plant phosphorus. Our results may thus contribute to water conservation, increased yields and the development of strategies for efficient phosphorus fertilization. © 2024 Society of Chemical Industry.

Differential Strategies of Two Arbuscular Mycorrhizal Fungi Varieties in the Protection of Lycium ruthenicum under Saline-Alkaline Stress.

To delve into the growth and physiological adaptations exhibited by the economically vital black wolfberry (Lycium ruthenicum) upon inoculation with arbuscular mycorrhizal fungi (AMF) under varying levels of saline-alkaline stress A series of pot experiments were conducted in a gradient saline-alkaline environment (0, 200, 400 mM NaCl: NaHCO3 = 1:1). One-year-old cuttings of black wolfberry, inoculated with two AMF species-Funneliformis mosseae (Fm) and Rhizophagus intraradices (Ri)-served as the experimental material, enabling a comprehensive analysis of seedling biomass, chlorophyll content, antioxidant enzyme activities, and other crucial physiological parameters. This study demonstrated that both Fm and Ri could form a symbiotic relationship with the root of Lycium ruthenicum. Notably, Fm inoculation significantly bolstered the growth of the underground parts, while exhibiting a remarkable capacity to scavenge reactive oxygen species (ROS), thereby effectively mitigating membrane oxidative damage induced by stress. Additionally, Fm promoted the accumulation of abscisic acid (ABA) in both leaves and roots, facilitating the exclusion of excess sodium ions from cells. Ri Inoculation primarily contributed to an enhancement in the chlorophyll b (Chlb) content, vital for sustaining photosynthesis processes. Furthermore, Ri's ability to enhance phosphorus (P) absorption under stressful conditions ensured a steady influx of essential nutrients. These findings point to different strategies employed for Fm and Ri inoculation. To holistically assess the saline-alkaline tolerance of each treatment group, a membership function analysis was employed, ultimately ranking the salt tolerance as Fm > Ri > non-mycorrhizal (NM) control. This finding holds paramount importance for the screening of highly resilient Lycium ruthenicum strains and offers invaluable theoretical underpinnings and technical guidance for the remediation of saline-alkaline soils, fostering sustainable agricultural practices in challenging environments.

Fulvic acid application increases rice seedlings performance under low phosphorus stress

BackgroundFulvic acid enhances plant growth and interacts synergistically with phosphate fertilizer to alleviate the agricultural production problem of low phosphorus fertilizer utilization efficiency. However, the underlying mechanism of its action remains poorly understood. In this study, we investigated the impact of fulvic acid application with varying concentrations (0, 40, 60, 80 and 120 mg/L) on rice performance in plants grown in a hydroponic system subjected to low phosphorus stress. The rice growth phenotypes, biomass, root morphology, phosphorus uptake, and the impact of fulvic acid on the rhizosphere environment of rice, were assessed.ResultsThe findings showed that adding appropriate concentrations of exogenous fulvic acid could promote the growth performance of rice under low phosphorus stress. Particularly at T1 (40 mg/L) and T2 (60 mg/L) over the control effectively increased rice biomass by 25.42% and 24.56%, respectively. Fulvic acid treatments stimulated root morphogenesis, up-regulated phosphate transporter genes, and facilitated phosphorus absorption and accumulation. Especially T1 (20.52%), T2 (18.10%) and T3 (20.48%) treatments significantly increased phosphorus uptake in rice, thereby alleviating low phosphorus stress. Additionally, fulvic acid elevated organic acids concentration in roots and up-regulated plasma membrane H+-ATPase genes, promoting organic acids secretion. This metabolic alteration can also alleviate low phosphorus stress in rice.ConclusionsThe effect of exogenous fulvic acid on physiological indicators is concentration-dependent under low phosphorus stress, enhances rice performance and reduces reliance on phosphorus fertilizer. This provides new insights to shed light on the mechanism of alleviating low phosphorus stress in rice through fulvic acid application, an eco-friendly tool.

Absorption and Xylem Transport of 33P-Labeled Phosphorus in Nodulated Soybean Plants

Phosphorus (P) is an essential macro-element for plants, and understanding the characteristics of the absorption and transport of P in crops is essential. The low availability of P restricts the growth, nitrogen fixation, and yield of soybean plants. In this research, the radioisotope 33P was supplied to the culture solution to trace the absorption and transport of P in nodulated soybean plants monitored using an imaging plate. The absorption rate of 33P was almost the same under the light and dark conditions. The absorption rate of 33P in the decapitated roots was near to that of the intact plants under light. These results indicate that the P absorption is not affected by evapotranspiration over a short time period. Conversely, the 33P transport from the roots to the shoot was significantly lower under dark conditions than it was under light conditions, although some 33P reached the top of the shoots under both the light and dark conditions. The transport of P to the shoots depends on the transpiration supplemented by the root pressure. The multiplication value of the 33P concentration in the xylem sap and transpiration rate was almost equivalent to the transport rate of 33P in the intact shoots. This value may be adaptable and used to estimate the transport rate of P for the diagnosis.

The feasibility and efficiency of fly ash as an absorbent for phosphate removal

Abstract Phosphorus is an essential nutrient required for the growth and development of plants, animals, and microorganisms. However, excessive phosphorus in wastewater can cause detrimental effects on aquatic ecosystems, leading to eutrophication and harmful algal blooms. Thus, the removal of phosphorus from wastewater is crucial to protect water bodies and maintain environmental sustainability. The removal mechanism of phosphorus by fly ash involves both physical and chemical processes. The high surface area and porosity of fly ash facilitate the adsorption of phosphorus molecules onto its surface, while the presence of calcium and aluminium oxides in fly ash enables the formation of stable precipitates with phosphorus in the solution. Numerous studies have been conducted to investigate the feasibility and efficiency of fly ash as an absorbent for phosphorus removal. These studies have demonstrated that fly ash can effectively remove phosphorus from wastewater, achieving removal efficiencies of up to 90%. The performance of fly ash as an absorbent can be enhanced through various strategies, such as modification with reactive substances or combining it with other materials. Objective of this study is parameters that effect on phosphorus absorption using fly ash as absorbent. The results showed that increasing pH decrease % removal.

MAPK signaling pathway participates in the regulation of intestinal phosphorus and calcium absorption in broiler chickens via 1,25-dihydroxyvitamin D3

Four experiments were performed to investigate the role of the mitogen-activated protein kinase (MAPK) signaling pathway in intestinal absorption of phosphorus (P) and calcium (Ca) in broiler chickens. Experiment 1 assessed how dietary levels of 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) influence the gene expression of intestinal P and Ca transporters in broilers. Experiment 2 evaluated the effects of 1,25(OH)2D3 administered via intraperitoneal injection on the extracellular signal-regulated kinase (ERK) and p38 mitogen-activated protein kinase (p38MAPK) signaling pathways. Experiments 3 and 4 investigated the effect of ERK and p38MAPK inhibitors on the expression of intestinal P and Ca transporters. The findings demonstrated that broilers (1-21 days old) fed a 1,25(OH)2D3-deficient diet (0.625 µg/kg) exhibited reduced body weight, tibia P and Ca levels, and mRNA levels of P transporters (NaPi-IIb, PiT-1, and PiT-2), Ca transporters (NCX1, PMCA1b, and CaBP-D28k), vitamin D receptors (VDR), ERK, and p38MAPK in the duodenum (Experiment 1) (P < 0.05). By comparison, the growth, bone quality, and mRNA levels of genes (except for duodenal NaPi-IIb) in broilers were similar to those in broilers fed the control diet when dietary 1,25(OH)2D3 was adequate (5 µg/kg) (Experiment 1) (P > 0.05). After intraperitoneal injection of 1,25(OH)2D3, the mRNA level of jejunal NaPi-IIb and the protein level of p-p38MAPK/t-p38MAPK in broilers (9-14 days old) decreased (P < 0.05), whereas the mRNA level of CaBP-D28k and the protein level of p-ERK/t-ERK increased (Experiment 2) (P < 0.05). The mRNA and protein expression of jejunal NaPi-IIb and the protein expression of CaBP-D28k in broilers (9-17 days old) treated with the ERK inhibitor PD98059 were greater than those in the control group (Experiment 3) (P < 0.05). Similarly, compared with control broilers, broilers (9-17 days old) treated with the p38MAPK inhibitor SB203580 showed elevated mRNA expression of jejunal NaPi-IIb and CaBP-D28k (Experiment 4) (P < 0.05). These results suggest that adequate supplementation with 1,25(OH)2D3 (5 µg/kg) can restore broiler growth and bone quality by upregulating the transcription of genes involved in intestinal P and Ca absorption. Additionally, the ERK and p38MAPK signaling pathways are implicated in the modulatory effect of 1,25(OH)2D3 on the absorption of P and Ca in broilers.

LITERATURE REVIEW: PERANAN ACAULOSPORA TERHADAP PENYERAPAN FOSFOR PADA AKAR TANAMAN SINGKONG

Mycorrhiza is a form of mutualistic symbiosis between plant roots (rhiza) and fungi (mykes). Plants will get various kinds of nutrients, especially phosphorus (P) from their mycorrhiza, while mycorrhiza will get carbon (C) from plants. Phosphorus is a macro nutrient that plants must have. Phosphorus has important functions in plants, namely during respiration, photosynthesis, energy transport, energy storage, cell enlargement, cell division, as well as processes within plants, stimulating plant root development and germination. Mycorrhiza is grouped into three types, namely ectendomycorrhiza, ectomycorrhiza, and endomycorrhiza. This journal review uses the SLR or Systematic Literature Review method with the aim of knowing the meaning of mycorrhiza, characteristics, enzymes that play a role, the mechanism of phosphorus absorption in mycorrhiza, mycorrhizal colonization, and mycorrhizal symbiosis in cassava plants. This article discusses mycorrhiza, one of the mycorrhizal genera, namely Acaulospora, which is included in the endomycorrhiza or Vesicular Arbuscular Mycorrhiza (VAM). The spores are characterized by a brownish yellow color and a rough surface like orange peel. The phosphatase enzyme is produced by Acaulospora hyphae. This enzyme helps increase the amount of P nutrients that are useful for plants by releasing the fixation of P nutrients from Al, Fe, and Ca in the soil, so that phosphate elements are available to plants.

Эколого-агрохимическая оценка устойчивости серой лесной почвы к неблагоприятному воздействию и ее плодородие для выращивания сельскохозяйственных культур

The article presents research based on long-term field stationary experiments conducted at the agrotechnological experimental station of the UNIC "Agrotechnopark" to study the complex cultivation of gray forest soil of heavy loamy soil. The most favorable phosphate regime is soil with relatively high buffer properties, due not to the absorption of phosphorus by the solid phase, but its desorption. It was found that if the total acidification buffering capacity for pH intervals lies in the range of 9-11 mM–eq/100 g, then an average level of soil stability is achieved. In this case, the absorbed bases should be at least 20 mg-eq/100 g. It is not recommended to reduce the acidification buffering capacity to values less than 9 mM-eq/100 g. In long–term experiments with potash fertilizers, reliable increases in crop yields were established, which varied depending on the type of fertilizer and rotation in the range of 1.8 - 6.5 c/ha of grain units. On average, over 8 rotations, the increase was 5.0 c/ha of grain units. In the variant without fertilizers, the crop rotation productivity was about 24 c/ha of grain units. Keywords: GRAY FOREST SOIL, BUFFERING, NON-CHERNOZEM ZONE, PRODUCTIVITY, ORGANIC FERTILIZER, FERTILITY

Growth, Yield and Uptake of P Four Variety Soybeans on Biocomposted Coastal Lands

Soybeans (Glycine max (L.) Merrill) are a popular food commodity in Indonesia, but domestic production is still insufficient to meet the demand. To increase production, planting superior varieties on coastal land could be a viable solution. Despite the challenging biological, physical, and chemical conditions of coastal land, nutrients such as phosphorus (P) are crucial for enhancing production. Typically, the phosphorus content in the soil is lower than that of nitrogen (N) and potassium (K), and the ability of soybean plants to absorb phosphorus varies among varieties. This research aims to compare the growth, yield, and phosphorus uptake of four soybean varieties on biocomposted coastal land. The study was conducted using a Randomized Complete Block Design (RCBD) with a single factor consisting of four soybean varieties: Wilis, Anjasmoro, Malika, and Detam 1. The results indicate that the Anjasmoro, Wilis, Detam 1, and Malika varieties exhibit similar responses in phosphorus absorption. However, the Anjasmoro and Wilis varieties demonstrate better adaptability compared to Malika and Detam 1, as evidenced by their superior growth and yield. This suggests that Anjasmoro and Wilis have a higher capability to adapt to the challenging conditions of coastal land. Therefore, these two varieties are recommended for cultivation in coastal areas to boost soybean productivity, ensuring the sustainability and stability of the national food supply.