Lake Erie is threatened by eutrophication and harmful algal blooms due to excess nutrient loading from agricultural sources. To reduce nutrient loading to Lake Erie, widespread adoption of agricultural conservation practices (ACPs) has been proposed. However, identifying appropriate and effective locations for ACP placement has been challenging. Another challenge is understanding how effective the ACPs are in reducing nutrient loading and achieving water quality goals. Therefore, identifying the most effective ACPs, as well as spatially optimal placement of ACPs to achieve the maximum environmental benefit, is of paramount importance. The main objective of this study was to integrate the Agricultural Conservation Planning Framework (ACPF) with the Soil and Water Assessment Tool (SWAT) to assess the potential effectiveness of ACPs developed by ACPF in reducing phosphorous losses from an agriculturally dominated small watershed within the Western Lake Erie Basin. ACPF was used to develop a series of ACP opportunity plans, which were then integrated into a calibrated SWAT model. SWAT simulation of ACPF developed ACP opportunity plans for grassed waterways (GWs), contour buffer strips (CBSs), water and sediment control basins (WASCOBs), nutrient removal wetlands (NRWs), and farm ponds (FPs) revealed various reductions in sediment, soluble reactive phosphorus (SRP), and total phosphorus (TP) at the watershed-scale. The simulation of individual ACP opportunity plans revealed that GW resulted in the greatest annual average SRP and TP reductions (19% and 30%, respectively), followed by CBS (16% and 22%), and WASCOB (13% and 16%); NRWs were the most effective at reducing sediment (56%) but increased SRP (27%). Combined GW, CBS, and WASCOB opportunity plans resulted in the greatest reduction of SRP (34%), while the combination of all ACP opportunity plans resulted in the greatest reduction of TP (49%) and sediment (78%).