Phosphate Inhibition Research Articles

Preparation and characterization of a novel active sediment capping material (geopolymer) for inhibiting phosphate releasing from sediment

In situ remediation of sediment can effectively control the release of phosphate in sediment and improve water eutrophication. The essential question of this remediation techniques lies in the development of stable, high-efficiency, low-cost and easily available active sediment capping materials. This study synthesised a novel sediment capping material using bulk solid waste, and phosphate inhibition mechanism of the materials was explored. Results indicated that GSCM was prepared under the conditions of NaOH concentration of 3M, hydrothermal temperature of 160℃, hydrothermal time of 36h and the mass ratio of 40wt.% SS to 60wt.% FA. The result of batch adsorption and compressive strength test suggested that phosphate adsorption capacity and compressive strength of GSCM were 2.15mg/g and 24.20MPa, respectively. The characterization result showed that GSCM was composed of sodium zeolite, riversideite, grossular and fayalite, exhibiting a uniformly distributed slit mesoporous structure. The in-situ inhibition efficiency of GSCM to P ranged from 76.65% to 86.72%, exceeding that of commercial zeolite. The in-situ inhibition mechanism was controlled by sodium zeolite and riversideite, and concluded by the following:1) Substitution between [SiO4] tetrahedra (within the sodium zeolite structure), -OH (on the surface of materials) and [PO4], 2) Coordination of [PO4] tetrahedra with Al active site within the sodium zeolite structure, 3) Precipitation reaction between phosphate and slow-release of Ca2+ from the riversideite.

Intercalation modified nano zirconium phosphate inhibitor for inhibiting coal spontaneous combustion

The synthesis and characterization of a modified zirconium phosphate (MZrP) nano-inhibitor for spontaneous coal combustion inhibition were explored through isooctylamine intercalation. Using oxidation experiment, FTIR and SEM, the variation rules of the characteristic parameters of the different coal samples were investigated. It was observed that MZrP exhibited enhanced dispersion within the coal matrix post-intercalation modification. As the MZrP concentration increased, there was a notable decrease in oxygen consumption rate, gas production concentration, and active group content in the coal, alongside a significant increase in apparent activation energy and inhibition efficacy. This enhanced inhibition is attributed to two primary mechanisms. Firstly, the hydrophilic nature of MZrP allows for its uniform distribution on the coal surface and within internal pores, creating a dense carbonized layer. This layer effectively retains moisture and isolates oxygen, enhancing physical inhibition. Secondly, MZrP inhibitor is thermally decomposed into phosphoric acid and its phosphoric acid derivatives, which can effectively capture the H- and -OH in the coal, and strengthens the inactivation of its reactive free radicals. The optimal inhibition was observed in coal samples treated with 6 wt% MZrP, exhibiting an average inhibition rate of 62.2 %, coupled with the lowest rates of gas production and oxygen consumption.

Downregulation of IL-8 and IL-10 by LRRC8A Inhibition through the NOX2-Nrf2-CEBPB Transcriptional Axis in THP-1-Derived M2 Macrophages.

M2-polarized, tumor-associated macrophages (TAMs) produce pro-tumorigenic and angiogenic mediators, such as interleukin-8 (IL-8) and IL-10. Leucine-rich repeat-containing protein 8 members (LRRC8s) form volume-regulated anion channels and play an important role in macrophage functions by regulating cytokine and chemokine production. We herein examined the role of LRRC8A in IL-8 and IL-10 expression in THP-1-differentiated M2-like macrophages (M2-MACs), which are a useful tool for investigating TAMs. In M2-MACs, the pharmacological inhibition of LRRC8A led to hyperpolarizing responses after a transient depolarization phase, followed by a slight elevation in the intracellular concentration of Ca2+. Both the small interfering RNA-mediated and pharmacological inhibition of LRRC8A repressed the transcriptional expression of IL-8 and IL-10, resulting in a significant reduction in their secretion. The inhibition of LRRC8A decreased the nuclear translocation of phosphorylated nuclear factor-erythroid 2-related factor 2 (Nrf2), while the activation of Nrf2 reversed the LRRC8A inhibition-induced transcriptional repression of IL-8 and IL-10 in M2-MACs. We identified the CCAAT/enhancer-binding protein isoform B, CEBPB, as a downstream target of Nrf2 signaling in M2-MACs. Moreover, among several upstream candidates, the inhibition of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase 2 (NOX2) suppressed the Nrf2-CEBPB transcriptional axis in M2-MACs. Collectively, the present results indicate that the inhibition of LRRC8A repressed IL-8 and IL-10 transcription in M2-MACs through the NOX2-Nrf2-CEBPB axis and suggest that LRRC8A inhibitors suppress the IL-10-mediated evasion of tumor immune surveillance and IL-8-mediated metastasis and neovascularization in TAMs.

Structural and kinetic characterization of DUSP5 with a Di-phosphorylated tripeptide substrate from the ERK activation loop

Introduction: Dual specific phosphatases (DUSPs) are mitogen-activated protein kinase (MAPK) regulators, which also serve as drug targets for treating various vascular diseases. Previously, we have presented mechanistic characterizations of DUSP5 and its interaction with pERK, proposing a dual active site.Methods: Herein, we characterize the interactions between the DUSP5 phosphatase domain and the pT-E-pY activation loop of ERK2, with specific active site assignments. We also report the full NMR chemical shift assignments of DUSP5 that now enable chemical shift perturbation and dynamics studies.Results and Discussion: Both phosphates of the pT-E-pY tripeptide are dephosphorylated, based on 31P NMR; but, steady state kinetic studies of the tripeptide both as a substrate and as an inhibitor indicate a preference for binding and dephosphorylation of the phospho-tyrosine before the phospho-threonine. Catalytic efficiency (kcat/Km) is 3.7 M−1S−1 for T-E-pY vs 1.3 M−1S−1 for pT-E-Y, although the diphosphorylated peptide (pT-E-pY) is a better substrate than both, with kcat/Km = 18.2 M−1S−1 . Steady state inhibition studies with the pNPP substrate yields Kis values for the peptide inhibitors of: 15.82 mM (pT-E-Y), 4.932 mM (T-E-pY), 1.672 mM (pT-E-pY). Steady state inhibition studies with pNPP substrate and with vanadate or phosphate inhibitors indicated competitive inhibition with Kis values of 0.0006122 mM (sodium vanadate) and 17.32 mM (sodium phosphate), similar to other Protein Tyrosine Phosphatases with an active site cysteine nucleophile that go through a five-coordinate high energy transition state or intermediate. Molecular dynamics (MD) studies confirm preferential binding of the diphosphorylated peptide, but with preference for binding the pY over the pT reside in the catalytic site proximal to the Cys263 nucleophile. Based on MD, the monophosphorylated peptide binds tighter if phosphorylated on the Tyr vs the Thr. And, if the starting pose of the docked diphosphorylated peptide has pT in the catalytic site, it will adjust to have the pY in the catalytic site, suggesting a dynamic shifting of the peptide orientation. 2D 1H-15N HSQC chemical shift perturbation studies confirm that DUSP5 with tripeptide bound is in a dynamic state, with extensive exchange broadening observed—especially of catalytic site residues. The availability of NMR chemical shift assignments enables additional future studies of DUSP5 binding to the ERK2 diphosphorylated activation loop.Summary: These studies indicate a preference for pY before pT binding, but with ability to bind and dephosphorylate both residues, and with a dynamic active site pocket that accommodates multiple tripeptide orientations.

Development of a 2D Network of Zinc Organophosphate with an 8-Membered Ring Core Having Corrosion Resistance Properties.

A 2D framework, i.e., zinc organophosphate [Zn(tmbiph)(solv)]2 (tmbiphH4 = tetramethyl-[1,1'-biphenyl]-4,4'-diyl bis(dihydrogen phosphate); solv = 2H2O) with an eight-membered ring core has been developed. The hierarchical and rational design of the zinc organophosphate has been achieved by carefully designing an organic bisphosphate ligand with two phosphate monoester groups connected through extended aromatic rings with methyl groups at ortho positions. The development of the 2D network does not require any ancillary ligand due to participating two phosphate groups in the coordination. The molecular structure of the zinc phosphate material was determined using single-crystal X-ray diffraction technique. The corresponding Co and Cu organophosphate materials have also been obtained using the same synthetic method; however, these compounds could not be confirmed structurally due to nondiffractive crystal quality. The structure of zinc organophosphate obtained through single-crystal X-ray diffraction has also been supported by theoretical calculations using density function theory. Furthermore, the zinc organophosphate material has been employed as an corrosion inhibitor for mild steel. The effect of the zinc phosphate framework on mild steel in 1 M HCl was investigated using polarization and electrochemical impedance spectroscopy. This study suggests that such phosphate inhibitors can be employed in the form of a thin protective layer on the electrode surface.

Study on the repair behavior of passive film of iron-based amorphous coatings by eco-friendly phosphate inhibitors

The passivation ability of the coating can be improved by adjusting the elements proportion in the Iron-based Amorphous Coating and the spraying process, but the process is complicated and costly. In this study, it was found for the first time that Iron-based Amorphous Coatings can quickly form passive films in phosphate atmosphere, which simplifies the coating strengthening process. Electrochemical measurements, XPS, AFM, FTIR, and SEM-EDS were used to investigate the inhibition mechanism of eco-friendly phosphates on the dynamic dissolution of passive film of Fe-based amorphous coating. When the coating was soaked in 3.5 wt% NaCl + 0.1 M solution, the iron phosphate compound on the coating surface promoted the repair and growth of the passive film, and the film thickness increased by more than 32.9 %. The increase of Fe2+/Fe3+ and (O2-+H2O)/OH- in the passive film means that more insoluble Fe-Cr compound growth significantly reduces the donor density (Nd) in the passive film and inhibits the degradation rate of the passive film in the corrosive environment.

Development of a facile one-pot synthesized fluorescent green polyesters as cooling water antiscalants for industrial application

Currently, antiscalants used in cooling water are expected to be environmental-friendly, good scale inhibitive, fluorescence tracing, and industrial-friendly. Aliphatic biodegradable photoluminescent polymers (BPLPs) synthesized from citric acid, aliphatic diols, and various amino acids are fluorescent polymers containing heteroatom six-membered conjugated ring chromophores. Their cross-linked BPLPs have potential for use as implant or device materials, and vivo bioimaging probes. However, so far, no one has found that BPLPs can be used as efficient antiscalants for cooling water. In this work, we demonstrate a facile one-pot microwave-assisted synthesis of BPLPs from cost-effective materials. The products were characterized by Fourier transforms infrared spectroscopy, 1H and 13C nuclear magnetic resonance spectroscopy, gel permeation chromatography, and fluorescence spectroscopy. Thus prepared BPLPs can be directly used as a novel type green fluorescent antiscalant without further purification. They have an excellent calcium phosphate inhibition efficiency that can reach 98.6 % with a dosage of 25 mg·L−1 in the static scale inhibition test at 80 °C.

Enhanced-Efficiency Urea Coated with Ground Phosphate Rock Powder, Inhibitor and Epoxy Resin: Preparation and Effects on Soil Nitrogen Supply Capacity, Wheat Yield and Nitrogen Use Efficiency

Enhanced-Efficiency Urea Coated with Ground Phosphate Rock Powder, Inhibitor and Epoxy Resin: Preparation and Effects on Soil Nitrogen Supply Capacity, Wheat Yield and Nitrogen Use Efficiency

Chrysophyllum Albidum extract as a new and green protective agent for metal

ABSTRACT Gravimetric measurements and potentiodynamic polarization techniques were employed to investigate the protective performance of the metal in 1 M HCl and 0.5 M H2SO4 solutions by the C. albidum leaf extract at different concentrations (400-1600 mg/L) and time periods (1-4 days). The results showed the highest protection ability of over 96-97%, with inhibition increasing with higher extract concentrations. Polarization studies indicated a mixed inhibition mechanism, reducing both anodic dissolution and cathodic hydrogen evolution rates. Gas chromatography–mass spectrometry (GC–MS) analysis identified major organic constituents in the extract. Thermodynamic data fit the Langmuir isotherm model, suggesting chemisorptive adsorption of inhibitor molecules on the mild steel surface. Computational methods, including density functional theory (DFT), molecular dynamics simulations, and quantum chemical calculations, provided insight into inhibitor-metal interactions and adsorption behaviour at the molecular level. Overall, the study establishes C. albidum leaf extract as an effective, sustainable alternative to commonly used toxic chromate and phosphate inhibitors. It performs competitively even at low concentrations and in environments. The promising green inhibition properties, combined with the extract's nontoxicity and abundance from a natural source, suggest its potential as a cost-effective corrosion protection solution, especially for applications in developing economies.

New insights of inorganic phosphate inhibitors for flotation separation of calcium-bearing minerals

New insights of inorganic phosphate inhibitors for flotation separation of calcium-bearing minerals

In situ-polymerized and nano-hybridized Ti3C2-MXene with PDA and Zn-MOF carrying phosphate/glutamate molecules; toward the development of pH-stimuli smart anti-corrosion coating

A smart anti-corrosion epoxy coating (EPC) was designed on the basis of polydopamine (PDA), and zeolitic imidazolate framework8 (ZIF8) tailored MXene (Ti3C2) sheets, which were loaded with two different corrosion inhibitors, namely phosphate and glutamate ions. The utilization of these inhibitor-loaded hybrid carriers in EPC yielded self-healing properties and prolonged resistance against corrosion. First, characterization tests including FT-IR, Raman, XRD, XPS, BET, TGA, SEM, and HRTEM were performed to confirm the synthesis of the constructed containers. Then, the inhibition efficiency and mechanism of the container were investigated via ICP and electrochemical (EIS and polarization) analyses. The synthesized PM-ZP and PM-ZG containers loaded with phosphate and glutamate inhibitors were proven to have a promising efficiency in reducing the corrosion of the metal, as the inhibition efficiency in the polarization test was demonstrated to be 42.17 and 60.31 %, respectively. The impedance values in the EIS test regarding solution media showed a considerable increment in PM-ZP (6511.3 Ω.cm2@3h) and PM-ZG (5438.3 Ω.cm2@5h) compared to the unprotected steel (2081.0 Ω.cm2@24 h). The self-healing potential of the nanocomposite containing PM-ZP and PM-ZG samples was manifested through the EIS test, FE-SEM as well as EDX maps of coatings with artificially produced scratches. The intact coatings’ long-term barrier characteristics were also investigated with an EIS test and impedance values > 40 Ω.cm2 and log|Z|10mHz > 10.5 justified the high performance of epoxy composites containing PM-ZP and PM-ZG synthesized carrier after 90 days of immersion in aggressive media. Pull-off, salt spray, and cathodic disbonding tests revealed that coatings containing PM-ZP and PM-ZG carriers outperformed epoxy coatings without corrosion inhibitors.

Assessing the effectiveness of 3, 4-dimethylpyrazole phosphate (DMPP) inhibitor in mitigating N2O emissions from contrasting Cd-contaminated soils

Improving nitrogen use efficiency of chemical fertilizers is essential to mitigate the negative environmental impacts of nitrogen. Nitrification, the conversion of ammonium to nitrate via nitrite by soil microbes, is a prominent source of nitrogen loss in soil systems. The effectiveness of nitrification inhibitors in reducing nitrogen loss through inhibition of nitrification is well-documented, however, their efficacy in heavy metals-contaminated soils needs thorough investigations. The current study assessed the efficacy of nitrification inhibitor 3, 4-dimethylpyrazole phosphate (DMPP) in reducing nitrous oxide (N2O) emissions in cadmium (Cd) contaminated paddy and red soils under lab-controlled environment. Obtained results indicated the substantial reduction in N2O emissions with DMPP in paddy and red soil by 48 and 35 %, respectively. However, Cd contamination resulted in reduced efficacy of DMPP, thus decreased the N2O emissions by 36 and 25 % in paddy and red soil, respectively. It was found that addition of DMPP had a significant effect on the abundance of ammonia oxidizing bacteria (AOB) and archaea (AOA). Notably, the reduction in N2O emissions by DMPP varied with the abundance of AOB. Moreover, Cd pollution resulted in a significant (P < 0.05) reduction in the abundance of archaeal and bacterial amoA genes, as well as bacterial nirK, nirS, and nosZ genes. The combined treatment of Cd and DMPP had a detrimental impact on denitrifiers, thereby influencing the overall efficiency of DMPP. These findings provide novel insights into the application of DMPP to mitigate nitrification and its potential role in reducing N2O emissions in contaminated soils.

Control of Corrosion Rate of Metal Alloys: SS 304 And SS 201 Using Phosphate Inhibitor in Nacl 3.5%

Corrosion is metal damage caused by electrochemical reactions with its environment. This environment can be water, air, gas, acid solution, salt solution, and others. Metals are highly active in producing an oxide layer on the metal surface under the influence of an aerobic environment. Metal corrosion is a process where the action of the surrounding media damages metal materials by the introduction of corrosion ions. This study aims to determine the best conditions for phosphate in controlling the rate of corrosion and Knowing the inhibition of phosphate on controlling the rate of corrosion. The materials used were stainless steel 201, stainless steel 304, and phosphate as an inhibitor. Meanwhile the solution NaCl 3.5% as a corrosive media environment and distilled water. Based on this research, the best phosphate concentration in reducing the corrosion rate of stainless steel 201 and 304 is 100 ppm with a reduction in the corrosion rate of stainless steel 201 of 0.022312 mpy with an inhibition percentage of 89.68%, and a reduction in the corrosion rate of stainless steel 304 by 0.045694 mpy with the percentage of inhibition is 94.027%. The corrosion rate of 304 stainless steel is lower than that of 201 stainless steel caused by differences in the Cr element composition of each metal. Stainless steel 304 contains 18.24% Cr while stainless steel 201 contains 13.00% Cr. The lower the Cr content in stainless steel, the stainless steel will be more susceptible to corrosion.

Exploring biological activity and In-vitro anticancer effects of a new biomaterial derived from Schiff base isolated from Homarus americanus (Lobster) shell waste

In this study, chitin, a valuable biopolymer material, was extracted from lobster (Homarus americanus) shell waste using a chemical method. Afterward, the biopolymer was added to a mixture of the precursor (β-diketone and acidhydrazide) and monochloroacetic acid to prepare the biomaterial/hydrazone-based O-carboxymethyl chitosan Schiff base compounds. The prepared biomaterials were studied for potential biological applications, such as antimicrobial, antioxidant, wound healing, cytotoxicity, phosphate inhibition, and anticancer studies. The inhibition of alkaline phosphatase (ALP) enzyme was recorded using UV–Vis spectrophotometry. Wound healing studies of biopolymer materials and their derivatives were studied to RAW 264.7 (murine monocyte / macrophage) cells by scratch wound healing analysis. Additionally, using MTT analysis the in-vitro, anticancer efficacies (cell viability/cell proliferation/cytotoxicity) were conducted for SK-MEL-28 (human melanoma cells), MDA-MB-231 (human breast cancer cells), and the U87 (human glioma brain cells). The half maximal inhibitory concentration (IC50) values for SK-MEL-28 cells had 0.71 μg/mL at 24 h, and was a potent activity as compared to U-87 (35.98 μg/mL) and MDA-MB-231 (18.31 μg/mL) cells. The water-soluble / swelling nature of prepared biomaterials is utilized in the bio-ink formulation by the incorporation of the biopolymers such as cellulose, sodium alginate, gelatin, hyaluronic acid, and laponite RDS for potential biomedical applications.

Effects of the nutrient inhibition on the yield of DBPFPs by Microcystis aeruginosa.

The yield of three disinfection byproduct formation potentials (DBPFPs), including trichloromethane, dichloroacetic acid and trichloroacetic acid formation potential (TCMFP, DCAAFP and TCAAFP), by Microcystis aeruginosa under the nitrate and phosphate inhibition conditions was investigated. The results showed that excessive nitrate could inhibit the growth of M. aeruginosa, but the concentration of DBPFPs in the five fractions of algal metabolites, including hydrophilic extracellular organic matter (EOM), hydrophobic EOM, hydrophilic intracellular organic matter, hydrophobic intracellular organic matter and cell debris, only decreased slightly. Accordingly, the productivity of DBPFPs by M. aeruginosa increased by approximately 40% under the nitrate inhibition condition and the increased productivity of DBPFPs mainly came from EOM. The phosphate inhibition also performed a similar pattern with a lesser extent. The nutrient inhibition did not change the proportion of these three DPBFPs, and TCMFP accounted for approximately 87% of the total DBPFPs. The inhibition could promote M. aeruginosa to secrete more metabolites. However, the cyanobacteria tended to secrete more DBPFPs under the nitrate inhibition condition, which resulted in an increased specific DBPFP, while they tended to secrete more non-DBPFPs under the phosphate inhibition condition, which resulted in a decreased specific DBPFP.

Experimental evaluation of corrosion inhibitors for completion fluids in the petroleum production systems

Corrosion is the natural and continuous degradation of materials caused by either chemical, mechanical, or electrochemical reactions. Corrosion inhibitors may be added to the completion fluids to address corrosion problems efficiently. It is critical to add corrosion inhibitors in completion fluids, specifically under high-temperature conditions, since the corrosion rate is higher when the temperature is high. This corrosion process limits the life of the drill tools or the oil and gas well and causes formation damage. This research studied corrosion and corrosion inhibition treatments for five completion fluids, namely potassium chloride, sodium chloride, sodium bromide, calcium chloride, and calcium bromide. Phosphate and sulfite-based corrosion inhibitors were individually added to the completion fluids, and their corrosion properties were studied to tackle the corrosion issue. In addition, a mixture of phosphate-based and sulfite-based corrosion inhibitors in completion fluids was studied. Additionally, the experimental results recommend using divalent brines as they were identified as a better medium for lowering corrosion rate and conditions than the monovalent brines. A novel aspect of this study is that the materials leveraged for conducting experiments are also used in actual petroleum production field operations. The experiments demonstrate that the corrosion rate can be efficiently controlled at high temperatures in deeper wells.

Investigation of the inhibition behavior of an octacalcium phosphate as a green corrosion inhibitor against carbon steel in 3 % NaCl medium

The development of new corrosion inhibitors constitutes a significant endeavor for researchers. In this study, the efficacy of octacalcium phosphate (OCPa), an inorganic green corrosion inhibitor for carbon steel in a 3% NaCl solution, was evaluated. This investigation marks the first application of this compound in a saline environment. The phosphate inhibitor was synthesized through a double aqueous decomposition process in an equimolar solution of water and ethanol, and subsequently characterized using X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), chemical analysis, and SEM/EDX to ascertain its properties and morphology. Meanwhile, the electrochemical behavior was investigated using techniques such as potentiodynamic polarization (PDP) and electrochemical impedance spectroscopy (EIS) to assess the anticorrosive performance of the studied phosphates. Analysis of the carbon steel surface morphology of the resultant layer was conducted using SEM/EDX and FTIR. According to the electrochemical results, OCPa demonstrated an impressive inhibitory effect, with an efficiency reaching 93.1% at an optimal concentration of 50 ppm, exhibiting a cathodic type of inhibition. Moreover, the charge transfer resistance was found to be 2450 Ω.cm2 in the presence of 50 ppm of the inhibitor, as compared to 161.0 Ω.cm2 in the uninhibited solution. The thermodynamic parameters indicated an endothermic nature for the adsorption process in the presence of OCPa. Examination of the carbon steel surface morphology by SEM revealed the formation of a protective layer, serving to shield the metal from corrosion. The experimental findings underscore the potential of octacalcium phosphate as a promising inorganic green corrosion inhibitor for carbon steel in saline environments, opening new avenues for the exploration and development of environmentally benign anti-corrosive substances.

Tribo-corrosion behavior of C-steel in water-based emulsion drilling fluids containing green corrosion inhibitors: experimental and computational studies

This article reports a study on the corrosive wear of carbon steel in water-based emulsion drilling fluids containing green corrosion inhibitors, PEG-6 isotridecyl phosphate and PEG-2 oleamide. The latter was used for comparison purposes, which was previously demonstrated to exhibit good performance. The effectiveness of the drilling fluids with the inhibitors, especially PEG-6 isotridecyl phosphate, in suppressing tribo-corrosion was largely improved, evidenced by much lowered corrosion rate, coefficient of friction (COF) and tribo-corrosion, compared to those without the green inhibitors. The benefits of the inhibitors were influenced by the sliding speed (2, 3.5, 5 and 10 mm/s). With increasing the sliding speed from 2 mm/s to 5 mm/s, the corrosion rate decreased, but further increasing the sliding speed to 10 mm/s did not continuously reduce the corrosion rate. However, COF value of carbon steel was continuously decreased as the sliding speed increased from 2 to 10 mm/s. It was shown that the corrosion inhibitors resulted in a more protective film on the worn surfaces, and the adsorption equilibrium constants increased with respect to the sliding speed from 182.6 M−1 to 849.6 M−1 for PEG-6 isotridecyl phosphate and 6.70 M−1 to 28.1 M−1 for PEG-2 oleamide. First-principles calculations and molecular dynamics simulations demonstrated that the better performance of the PEG-6 isotridecyl phosphate inhibitor was ascribed to its stronger polymer film and higher adherence to the iron surface.

The spectrum of homologous recombination deficiency in cervical cancer: Is it also a cervical issue?

5543 Background: Tumors deficient in homologous recombination DNA damage repair (HR-DDR) demonstrate sensitivity to therapeutic agents targeting the HR-DDR pathway, such as poly-ADP ribose phosphate (PARP) inhibitors. The landscape of homologous recombination deficiency (HRD) is not well-characterized for cervical cancer; thus, we sought to analyze the prevalence of somatic pathogenic gene variants (PGVs) in HRD genes in cervical cancers. Methods: The American Association for Cancer Research’s (AACR) Project Genomics Evidence Neoplasia Information Exchange (GENIE) database version 13.0 was queried for all cervical cancer tumors via cBioPortal (http://genie.cbioportal.org). This is a publicly available, multi-institutional database of next-generation sequencing (NGS) genomic profiles of multiple tumors. PGV frequencies of 27 genes involved in HR-DDR were descriptively reported for cervical cancer tumors, with histologic stratification: ATM, ARID1A, ATRX, BRCA1, BRCA2, BARD1, BRIP1, BLM, BAP1, CHEK1, CHEK2, FANCA, FANCC, FANCD2, FANCE, FANCF, FANCG, FANCL, MRE11, NBN, PALB2, RAD50, RAD51, RAD51B, RAD51C, RAD51D, WRN. Results: A total of 857 cervical tumors from 817 patients were included for analysis. At least one somatic PGV in an HRD gene was found in 16.5% of all tumors (141/857). When stratified by histological subtypes with at least 25 tumors sequenced, highest frequencies of ≥1 PGV in HRD genes were observed in mucinous cervical cancer (13/66, 19.7%), cervical adenocarcinoma (48/262, 18.3%), squamous cell cervical cancer (53/349, 15.2%), adenosquamous cervical cancer (6/43, 14.0%) and neuroendocrine cervical cancers (5/42, 11.9%). Substantial rates of PGVs in HRD genes were also observed in histological subtypes with fewer than 25 sequenced tumors. Across all tumors, HRD genes with the highest frequencies of PGVs were ARID1A (9%), BAP1 (3%), ATM (1.6%) and BRCA1 (1.6%). Conclusions: NGS data demonstrate a substantial rate of somatic PGVs in HRD genes in cervical cancers, including among HPV related subtypes. These data suggest the need to expand routine functional HRD status assessment to cervical cancers in order to further characterize the landscape of HRD in these tumors. Furthermore, genetically driven clinical trials are warranted to evaluate the efficacy of HRD-targeted therapies such as PARP inhibitors, ataxia telangiectasia, and Rad-3 related kinase (ATR) inhibitors. [Table: see text]

The win-win effects of nitrification inhibitors on soil-crop systems: Decreasing carbendazim residues but promoting soil bacterial community diversities and stabilities and crop yields

Applying nitrogen (N)-cycling inhibitors is an effective measure to improve N fertilizer utilization efficiency, but the effects of N-cycling inhibitors on fungicide residues in soil-crop systems are unclear. In this study, nitrification inhibitors dicyandiamide (DCD) and 3, 4-dimethylpyrazole phosphate (DMPP) and urease inhibitor N-(n-butyl) thiophosphoric triamide (NBPT) were applied into agricultural soils with fungicide carbendazim applications. The soil abiotic properties, carrot yields, carbendazim residues, bacterial communities and their comprehensive relationships were also quantified. Compared to the control treatment, the DCD and DMPP significantly decreased soil carbendazim residues by 96.2% and 96.0%, and the DMPP and NBPT significantly reduced carrot carbendazim residues by 74.3% and 60.3%, respectively. The nitrification inhibitor applications also generated significant and positive effects on carrot yields and soil bacterial community diversities. The DCD application significantly stimulated soil Bacteroidota and endophytic Myxococcota and modified soil and endophytic bacterial communities. Meanwhile, the DCD and DMPP applications also positively stimulated the co-occurrence network edges of soil bacterial communities by 32.6% and 35.2%, respectively. The linear correlation coefficients between soil carbendazim residues and pH, ETSA and NH4+-N contents were − 0.84, − 0.57 and − 0.80, respectively. The nitrification inhibitor applications generated win-win effects on the soil-crop systems by decreasing carbendazim residues but promoting soil bacterial community diversities and stabilities and crop yields.