Arsenic (As) contamination is one of the major factors that contribute to environmental pollution and it plays havoc with sustainability of crop production. Nano-hydroxyapatite (nHAP) can be an effective phosphorus (P) source to cope with As toxicity. The mitigating effects of variable P sources on As toxicity in rice, sunflower and maize grown in alkaline soil were evaluated. Arsenic was applied at 0 and 20 mg kg-1 and P was applied at 0, 50 and 500 mg kg-1 in the form of Triple Super Phosphate (TSP) and nHAP. In addition, the same amount of phosphoric acid (PA) was also used in sunflower and maize experiments with the other P sources. The structural and functional properties of nHAP were determined by Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffraction (XRD), Scanning Electron Microscopy (SEM) and Energy-Dispersive X-Ray Spectroscopy (EDX). Stunted growth was observed in rice, and less in sunflower; maize remained unaffected by As toxicity. In the presence of As, a high dose of TSP (TSP500) and nHAP (nHAP500) significantly increased dry weights of rice plants compared with control plants. Arsenic concentrations in rice were decreased by about 25% in the presence of 50 mg kg-1 TSP (TSP50) and nHAP500 treatments. Phosphorus treatments had no significant effect on the As concentrations of sunflower, while in maize plants As levels increased with TSP500 treatment as compared with P0As0. In the absence of As, TSP500 and nHAP500 treatments significantly increased rice P concentration (2.80 and 2.27 g kg-1) whereas, in the presence of As, TSP500 significantly increased P concentration in rice plants as compared with P0. Arsenic had no significant effect on sunflower P concentration while the maize P concentration was decreased in the P0 treatment. It can be concluded that TSP and nHAP were effective in alleviating As toxicity in rice and sunflower. Comparative studies are further needed to determine the long-term effects of TSP and nHAP on prevention of As toxicity in field conditions.