Phocoenoides Dalli Research Articles

Phylogeography and population structure of the Dall's porpoise, Phocoenoides dalli, in Japanese waters revealed by mitochondrial DNA.

We investigated genetic diversity and phylogenetic relationships among three morphologically distinct populations of Dall's porpoise (Phocoenoides dalli) in Japanese waters by analyzing mitochondrial DNA variation. These populations, the Sea of Japan-Okhotsk dalli-type population, the truei-type population and the standard dalli-type population in the northwestern North Pacific, are clearly discriminated from each other by differences in the size of their white flank patch. A total of 479 bp of the mitochondrial control region and flanking tRNA genes was sequenced for 103 individuals. Haplotypic diversity was high (h = 0.968), but these haplotypes differed by only a few nucleotides (pi = 0.0106). Although many haplotypes were shared between populations, analysis of molecular variance (AMOVA) indicated genetic subdivision among the three populations (overall F (ST) = 0.023, P < 0.001; phi(ST) = 0.026, P = 0.029). Pairwise comparisons indicated a low but significant difference between the Sea of Japan-Okhotsk and the other two populations, whereas there was no significant difference between the latter. These results suggest that there is a close evolutionary relationship among these populations despite their consistent differences in coloration. This may reflect genetic polymorphism in the common ancestral population, which subsequently underwent a rapid divergence. The low genetic variability and haplotypic differentiation of the Sea of Japan-Okhotsk population suggest that it originated from a small population that colonized the Sea of Japan or that experienced population reduction when this Sea was isolated from the North Pacific in the last glacial period.

Moisture content in Dall's porpoise ( Phocoenoides dalli) tissues: a reference base for conversion factors between dry and wet weight trace element concentrations in cetaceans

Concentration of trace elements measured by dry weight basis has become more commonly used in recent studies on cetaceans than wet weight basis, which was used more in earlier studies. Because few authors present moisture content data in their papers, it is difficult to compare the concentrations of trace elements between various studies. Therefore, we felt that it would be useful if a reference conversion factor (CF) for tissue types could be found to convert between wet weight and dry weight data on trace element concentrations. We determined the moisture contents of 14 tissues of Dall's porpoise ( Phocoenoides dalli), and then, calculated the CF values for those tissues. Because the moisture content of each tissue differs from other tissues, it is necessary to use a specific CF for each tissue rather than a general CF for several tissues. We have also found that CF values for Dall's porpoise tissues are similar to the same tissues in other cetaceans. Therefore CF values from Dall's porpoise can be reliably used to convert between wet and dry weight concentrations for other cetacean tissues as reference data.

Specific accumulation and temporal trends of organochlorine contaminants in Dall’s porpoises ( Phocoenoides dalli) from Japanese coastal waters

Organochlorine compounds (OCs) such as polychlorinated biphenyls (PCBs), dichlorodiphenyltrichloroethane and its metabolites (DDTs), chlordane related compounds, hexachlorocyclohexane isomers (HCHs), hexachlorobenzene (HCB), tris(4-chlorophenyl) methane (TCPMe) and tris(4-chlorophenyl) methanol were determined in the blubber of Dall’s porpoises ( Phocoenoides dalli) collected from Japanese coastal waters in 1998/1999. Among OCs analyzed, PCBs were the predominant contaminants with concentrations ranging from 1.5 to 39 μg/g on a wet weight basis. OCs concentrations were relatively low and less variable in mature females possibly due to the lactational excretion from their bodies, while in males the concentrations were increased with body length. Concentrations and compositions of DDTs and HCHs showed significant differences between the truei-type population off the Pacific coast of northern Japan and dalli-type from the Sea of Japan/Okhotsk, suggesting its usefulness to discriminate different populations. OCs levels detected in truei-type porpoises collected in 1998/1999 were lower than those collected in 1984, except TCPMe. On the other hand, except DDTs, the residue levels of other organochlorines in dalli-type porpoises showed no significant decrease since 1984.

Evaluation of mitogen-induced responses in marine mammal and human lymphocytes by in-vitro exposure of butyltins and non-ortho coplanar PCBs

The effects of exposure to butyltin compounds (BTs: tributyltin; TBT, dibutyltin; DBT and monobutyltin; MBT) and non-ortho coplanar PCBs (IUPAC 77, 126 and 169) on marine mammals and human lymphocyte were evaluated. Peripheral blood mononuclear cells (PBMCs) isolated from Dall's porpoises (Phocoenoides dalli), bottlenose dolphins (Tursiops truncatus), a California sealion (Zalophus californianus), a larga seal (Phoca largha) and humans (Homo sapiens) were exposed at varying concentrations of BTs and coplanar PCBs. Concanavalin A (Con A)-stimulated mitogenesis found significantly suppressed (P<0.01) when the cells were exposed at 300 nM (89 ng/ml) of TBT and 330 nM of DBT (77 ng/ml), while MBT showed little cytotoxicity at treatment levels of up to 3600 nM (620 ng/ml). BTs concentrations in the liver of Dall's porpoises from Japanese coastal waters ranged between 81–450 ng/g for TBT and 200–1100 ng/g (wet wt.) for DBTs, which is greater than the cytotoxic levels registered in this study. In contrast, non-ortho coplanar PCBs did not suppress cell proliferation at concentrations of up to 30 nM (10 ng/ml). The residue levels of coplanar PCBs in the blubber of Dall's porpoises were 0.12–1.3 ng/g, which were one order of lower than those levels that do cell proliferation. When cells were exposed to a mixture of TBT/DBTand coplanar PCBs, the proliferation was significantly reduced to 33 nM DBT plus 34 nM CB-77 and 33 nM DBT plus 28 nM CB-169 mixtures, respectively. The investigations relating the contaminant-induced immunosuppression in marine mammals have been focused on persistent organochlorines such as PCBs, pesticides and dioxin compounds. However, this study suggested the possibility of BTs could also pose a serious threat to the immune functions in free-ranging marine mammals and humans.

Trace elements in skin of Dall's porpoises ( Phocoenoides dalli) from the northern waters of Japan: an evaluation for utilization as non-lethal tracers

Concentrations of Fe, Zn, Cu, Se, Mn, Mo, Hg, Cd, Cr, Ag, Pb, Sr and V were determined in skins of Dall's porpoises ( Phocoenoides dalli) of the Pacific coast truei-type population (PT population) ( N=45), and the Sea of Japan-Okhotsk dalli-type population (JD population) ( N=31) from the northern waters of Japan. Cutaneous Hg concentrations in both PT and JD populations were significantly correlated with age, indicating a possible alternative method of age estimation. A significant correlation was also noted between Hg concentrations in skin and liver, suggesting that biopsy samples of skin can provide a non-lethal surrogate for monitoring Hg contamination in this species. Trace element accumulation patterns differed strongly between PT and JD populations, when analyzed by principal component analysis, suggesting these patterns could be utilized as non-lethal tracers of population identification.

Global distribution of halogenated dimethyl bipyrroles in marine mammal blubber.

Four halogenated dimethyl bipyrroles (HDBPs), hypothesized to be naturally produced, were quantitated in marine mammal blubber from a number of species obtained from various locations worldwide. HDBPs were found in samples from all locations studied. Concentrations of total HDBPs (SigmaHDBPs) ranged from 0.4 ng/g lipid weight in ringed seals (Phoca hispida) from the White Sea to 2,540 ng/g lipid weight in Dall's porpoise (Phocoenoides dalli) from the northwestern North Pacific Ocean. At their highest levels, SigmaHDBPs made up 11% of the total quantitated organohalogen body burden of adult male Dall's porpoises. In two beluga (Delphinapterus leucas) data subsets, it was found that males contained significantly higher concentrations of SigmaHDBPs than females. No significant effects of age or sex on SigmaHDBPs were observed in harbor seal (Phoca vitulina) and bowhead whale (Balaena mysticetus) data subsets. The geographical distribution of concentrations did not resemble that of the ubiquitous anthropogenic organohalogen, polychlorinated biphenyl congener CB-153. Higher concentrations of HDBPs and different patterns of congeners were observed in samples from Pacific as opposed to non-Pacific Ocean influenced environments. Concentrations of HDBPs in beluga from the Arctic and St. Lawrence River were similar. Their high abundance in north Pacific Ocean biota and widespread occurrence suggest that HDBPs undergo extensive transport from a source located primarily in the Pacific Ocean. Evidence from HDBP congener patterns indicates that both ocean currents and atmospheric transport likely play a role in the movement of HDBPs. These results imply that HDBPs and anthropogenic organohalogens have different sources and support the natural production hypothesis.

GEOGRAPHICAL VARIATION OF THE PARASITE, PHYLLOBOTHRIUM DELPHINI (CESTODA), IN BALL'S PORPOISE, PHOCOENOIDES DALLI, IN THE NORTHERN NORTH PACIFIC, BERING SEA, AND SEA OF OKHOTSK

AbstractPrevalence of the larval cestode, Phyllobothrium delphini, was estimated from 2,445 Dall's porpoise, Phocoenoides dalli, from the incidental take of the Japanese high seas salmon drift‐net fishery in the northwestern North Pacific Ocean, Bering Sea, and a local hand harpoon fishery in the southern Sea of Okhotsk. Prevalence of P. delphini was 22.7% in the northwestern North Pacific Ocean and 1.4% in the Bering Sea. This parasite was not found in the southern Sea of Okhotsk. Geographical differences in the prevalence of P. delphini may be due, at least in part, to regional differences in abundance of elasmobranchs known to feed on marine mammals and suspected as hosts of the parasite. Estimated intensity of infection of individual porpoises by P. delphini was low (estimated mean intensity of 3.5 plerocercoids per animal). This is a low intensity of infection compared to other species of small cetaceans studied and may be due to both differences in regional abundance of elasmobranchs and the comparatively short life span of P. dalli.

Identification of novel cytochrome P450 1A genes from five marine mammal species

Marine mammals, being endangered by the chronic exposure of hydrophobic environmental contaminants as an assorting result of global pollution, are especially focused as indicators for organochlorine pollution. The use of contaminant-induced xenobiotic metabolizers, particularly P450 (CYP) 1A, in marine mammals can be effective as potential biomarkers of the contaminant exposure and/or toxic effects. In this study, we identified the first marine mammalian CYPs. Six novel CYP1A cDNA fragments were cloned from the livers of marine mammal species, minke whale ( Balaenoptera acutorostrata), dall's porpoise ( Phocoenoides dalli), steller sea lion ( Eumetopias jubatus), largha seal ( Phoca largha), and ribbon seal ( Phoca fasciata) by the method of reverse transcription/polymerase chain reaction (RT/PCR); two distinct fragments were from steller sea lion and one fragment each was obtained from the other species. Five of the fragments, one from each species, were classified in the subfamily of CYP1A1, and the other fragment cloned from steller sea lion was designated CYP1A2. Degenerate PCR primers were used to amplify the fragments from liver cDNAs. The deduced amino acid sequences of these fragment CYP1As showed identities ranging from 50.0 to 94.3% with other known vertebrate CYPs in the subfamily of CYP1A, including those from fish, chicken, and terrestrial mammals. The isolated fragments were used to construct a molecular phylogeny, along with other vertebrate CYP1A cDNAs cut down in size to the corresponding region of 265 bp in which those newly determined fragments were cloned. This phylogenetic analysis by the maximum parsimony method using the PHYLIP program suggests two distinct evolutional pathways for aquatic mammalian CYP1As, compatible to a conservative taxonomy. Pinniped genes are clustered together with dog gene, forming a carnivore group, and cetaceans form another branch. Identification of CYP1A genes in marine mammals will be an introductory step to provide new insights into the metabolic or toxicological functions of CYP1As in these animals.

Environmental Models of Cetacean Abundance: Reducing Uncertainty in Population Trends

Abstract: Trends in population abundance are often used to monitor species affected by human activities. For highly mobile species in dynamic environments, however, such as cetaceans in the marine realm, natural variability can confound attempts to detect and interpret trends in abundance. Environmental variability can cause dramatic shifts in the distribution of cetaceans, and thus abundance estimates for a fixed region may be based on a different proportion of the population each time. This adds variability, decreasing statistical power to detect trends and introducing uncertainty whether apparent trends represent true changes in population size or merely reflect natural changes in the distribution of cetaceans. To minimize these problems, surveys ideally would be based on species‐specific design criteria that optimize sampling within all relevant habitat throughout a species' range. Our knowledge of cetacean habitats is limited, however, and financial and logistic constraints generally force those surveying cetacean abundance to include all species within a limited geographic region. Alternately, it may be possible to account for environmental variability analytically by including models of species‐environment patterns in trend analyses, but this will be successful only if such models have interannual predictive power. I developed and evaluated generalized additive models of cetacean sighting rates in relation to environmental variables. I used data from shipboard surveys of Dall's porpoise ( Phocoenoides dalli) and short‐beaked common dolphins ( Delphinus delphis) conducted in 1991, 1993, and 1996 off California. Sighting rates for these two species are variable and can be partially accounted for by environmental models, but additional surveys are needed to model species‐environment relationships adequately. If patterns are consistent across years, generalized additive models may represent an effective tool for reducing uncertainty caused by environmental variability and for improving our ability to detect and interpret trends in abundance.

FORAGING STRATEGIES OF SYMPATRIC KILLER WHALE (ORCINUS ORCA) POPULATIONS IN PRINCE WILLIAM SOUND, ALASKA

AbstractKiller whales (Orcinus orca) feed on a wide variety of fish, cephalopods, and marine mammals throughout their cosmopolitan range; however, the dietary breadth that characterizes the species is not reflected in all populations. Here, we present the findings of a 14‐yr study of the diet and feeding habits of killer whales in Prince William Sound, Alaska. Two non‐associating forms of killer whale, termed resident and transient (Bigg et al. 1987), were identified. All prey seen taken by transients were marine mammals, including harbor seals (Phoca vitulina), Dall's porpoises (Phocoenoides dalli), Steller sea lions (Eumetopias jubatus), and harbor porpoises (Phocoena phocoena). Resident killer whales appeared to prey principally on salmon (Oncorhynchus spp.), preferring coho salmon (O. kisutch) over other, more abundant salmon species. Pacific herring (Clupea pallasi) and Pacific halibut (Hippocampus stenolepis) were also taken. Resident killer whales frequently were seen to interact in non‐predatory ways with Steller sea lions and Dall's porpoises, while transients were not. Differences in the social organization and behavior of the resident and transient killer whales in Prince William Sound are discussed in the light of the dietary differences documented here.

A note on radionuclide 137Cs and 40K concentrations in Dall’s porpoises, Phocoenoides dalli, in coastal waters of Japan

Concentrations of artificial radionuclide 137Cs and natural radionuclide 4°K in Dall's porpoise, Phocoenoides dalli, from the Pacific coast of northern Japan in 1996 are presented. Concentrations of 137Cs in muscle tissue from two male Dall's porpoises were 0.153 ± 0.01 l and 0.234 ± 0.016 Bq kg-1 wetweight, and those of4°K were 104.0 ± 0.3 and 107.8 ± 0.9 Bq kg-1 wet weight, respectively. Concentration factors (CF, concentration in animal/concentration in sea water) for the two porpoises are 59 and 90 for 137Cs, respectively. These concentrations and CF values are within published ranges for marine fish in coastal waters. The CFs obtained in this study suggest that the trophic position of Dall's porpoises is similar to that of the large piscivorous fish in the marine community in coastal waters of Japan.

AGE, GROWTH, AND REPRODUCTIVE PATTERNS OF DALL'S PORPOISE (PHOCOENOIDES DALLI) IN THE CENTRAL NORTH PACIFIC OCEAN

AbstractLife history parameters were estimated for Dall's porpoise, Phocoenoides dalli, from biological specimens collected in the western Aleutian Islands, during 1981–1987. Of 2,033 males and 3,566 females examined, reproductive data were available for 1,941 males and 1,906 females; ages were determined for 813 males and 1,297 females. Female sexual maturity was based on the presence of one or more corpus on either ovary; 845 were sexually immature and 1,061 were sexually mature. Two estimates of female average age at sexual maturity (ASM) were 3.8 and 4.4 yr; average length at sexual maturity (LSM) was 172 cm. Males were considered sexually mature when evidence of spermatogenesis was detected; 1,136 were sexually immature and 805 were sexually mature. Two estimates of male ASM were 4.5 and 5.0 yr; LSM was 179.7 cm. Physical maturity was assessed for 246 males and 446 females by examining the degree of fusion in thoracic vertebral epiphyses. For both sexes, the average age at physical maturity was 7.2 yr. Average length at physical maturity was 202.6 cm for males and 192.7 cm for females. Average lengths of physically mature males (x̄= 198.1 cm, SE = 0.8566) and females (x̄= 189.7 cm, SE = 0.4002) were significantly different(P &lt; 0.0001). Early postnatal growth was rapid in both sexes. A secondary growth spurt in both mass and length was characteristic for both sexes; the increase in length preceded the mass increase by 1–2 yr. Average length at birth (LOB) was approximately 100 cm; birth mass averaged 11.3 kg (SE = 0.0772). By the time the umbilicus had healed (&lt;2 mo), the average length and mass had increased to 114.1 cm and 23.8 kg, respectively. Gestation period based on projections using LOB was 12 mo, but this was considered an overestimate. Calving was modal, centered in early July; an annual reproductive interval was indicated. Among the sexually mature females, 120 were pregnant, 55 were pregnant and lactating, 321 were pregnant with colostrum, and 33 were “resting.” By 3 July (95% CI =x̄ 1 d), 50% of births had occurred, during each of the seven years sampled. The ovulation rate was estimated at 0.914 ovulations per average reproductive year. Enlarged follicles and recent ovulations were observed in postpartum females in late July.

A note on cephalopods from the stomachs of Dall’s porpoises (Phocoenoides dalli) from the Northwestern Pacific and Bering Sea, 1978-1982

Cephalopod prey were identified from the stomachs of 100 Dall's porpoise (Phocoenoides dali) incidentally taken by commercial salmon gillnets and research vessels from 1978 to 1982 in the western North Pacific Ocean and Bering Sea, Fighty-four porpoise were collected Fi June and Joly during the salmon fishing season; the remainder were collected in August-Sepiember. Seven cephalopod families Gnoploteuthidae, Onychoteuthidae, Gonatidae, Histioteuthidae, Chiroreuthidae, Cranchiidac and Rolitaenidae) were identified in the Stomachs. Gonatids were the most abundant, comprising 98% of the beaks. Gonatopsis borealis was the most abundant species, occurring in 85 stomachs, The gonatids occur in meso- and epipclagic waters and many approach the surface at night when the porpoise are feeding.

SEASONAL PATTERNS IN THE ABUNDANCE AND DISTRIBUTION OF CALIFORNIA CETACEANS, 1991–1992

AbstractThis study presents a detailed seasonal comparison of the abundance and distribution of cetaceans within 100‐150 nmi (185‐278 km) of the California coast during 1991 and 1992. The results of a shipboard line‐transect survey conducted in July‐November 1991 (“summer”) were compared to those from aerial line‐transect surveys conducted in March‐April 1991 and February‐April 1992 (“winter”). Using a confidence‐interval‐based bootstrap procedure, abundance estimates for six of the eleven species included in the comparison exhibited significant (α= 0.05) differences between the winter and summer surveys. Pacific white‐sided dolphins (Lagenorhynchus obliquidens), Risso's dolphins (Grampus griseus), common dolphins (Delphinus spp.), and northern right whale dolphins (Lissodelphis borealis) were significantly more abundant in winter. The abundance of blue whales (Balaenoptera musculuss) and gray whales (Eschrichtius robustus) reflected well‐documented migratory patterns. Fin whales (B. physalus) were significantly more abundant during summer. No significant differences in seasonal abundance were identified for Dall's porpoises (Phocoenoides dalli), bottlenose dolphins (Tursiops truncatus), killer whales (Orcinus orca), sperm whales (Physeter macrocephalus), or humpback whales (Megaptera novaeangliae). Significant north/south shifts in distribution were found for Dall's porpoises, common dolphins, and Pacific white‐sided dolphins, and significant inshore/offshore differences were identified for northern right whale dolphins and humpback whales.

Occurrence and Morphological Comparisons of Campula oblonga (Digenea: Campulidae), including a Report from an Atypical Host, the Thresher Shark, Alopias vulpinus

New host records for Campula oblonga Cobbold, 1858 from the common dolphin, Delphinus delphis L., and from the thresher shark, Alopias vulpinus (Bonnaterre), are reported herein. Campulids have not been reported previously from a host that was not a marine mammal. The excellent condition and small size of the gravid specimen and the diet and natural history of the thresher shark lead us to conclude that the digene was acquired from the consumption of infected fish. Specimens of C. oblonga from these 2 hosts and from harbor porpoises, Phocoena phocoena (L.), and from Dall's porpoises, Phocoenoides dalli (True), are compared, and a wide variation in ranges and values for characters is noted. The synonymy of Campula folium Ozaki, 1935 with C. oblonga is further supported by the apparent effect of the host on the size of the trematodes. Due to the degree of variation in measurements and the numerous factors that may impact these values, we recommend the use of qualitative morphologic characters for the identification of C. oblonga.

Measurements of the nasal sacs of individual common dolphin, Delphinus delphis, and Dall's porpoise, Phocoenoides dalli, by means of silicon reconstruction.

Measurements of the nasal sacs of individual common dolphin, Delphinus delphis, and Dall's porpoise, Phocoenoides dalli, by means of silicon reconstruction.

An intergeneric hybrid in the family Phocoenidae

A 60-cm female fetus recovered from a Dall's porpoise (Phocoenoides dalli) found dead in southern British Columbia was fathered by a harbour porpoise (Phocoena phocoena). This is the first report of a hybrid within the family Phocoenidae and one of the first well-documented cases of cetacean hybridization in the wild. In several morphological features, the hybrid was either intermediate between the parental species (e.g., vertebral count) or more similar to the harbour porpoise than to the Dall's porpoise (e.g., colour pattern, relative position of the flipper, dorsal fin height). The fetal colour pattern (with a clear mouth-to-flipper stripe, as is found in the harbour porpoise) is similar to that reported for a fetus recovered from a Dall's porpoise to off California. Hybrid status was confirmed through genetic analysis, with species-specific repetitive DNA sequences of both the harbour and Dall's porpoise being found in the fetus. Atypically pigmented porpoises (usually traveling with and behaving like Dall's porpoises) are regularly observed in the area around southern Vancouver Island. We suggest that these abnormally pigmented animals, as well as the previously noted fetus from California, may also represent hybridization events.

Levels of organochlorine compounds, including PCDDS and PCDFS, in the blubber of cetaceans from the west coast of North America

Levels of organochlorine compounds (PCDD, PCDF, PCB and organochlorine pesticides) were determined in cetaceans collected from the west coast of North America between 1986 and 1989. The samples included gray whale ( Eschrichtius robustus), killer whale ( Orcinus orca), false killer whale ( Pseudorca crassidens), Risso's dolphin ( Grampus griseus) and Dall's porpoise ( Phocoenoides dalli) collected in British Columbia, and harbour porpoises ( Phocoena phocoena) collected in British Columbia and central California. TCDD and TCDF levels ranged from 1 to 8 ng kg −1 and 2.0 to 109 ng kg −1, respectively. The highest levels of PCDDs were found in the harbour porpoises; the levels of 1,2,3,6,7,8-HxCDD in the samples from Victoria, Campbell River and Qualicum River were 128, 128 and 62 ng kg −1, respectively. Five other 2,3,7,8-substituted dioxins and dibenzofurans were detected in the cetaceans at levels ranging from 1 to 10 ng kg −1. In addition to the 2,3,7,8-substituted congeners, several non 2,3,7,8-substituted congeners were detected. The patterns of the PCDDs and PCDFs in the British Columbia porpoises were consistent with implication of chlorophenols as the source of the PCDDs and PCDFs, which were either present in wood chips used in bleached kraft paper mills, or came from direct contamination by chlorophenols. No PCDDs or PCDFs were detected in the California samples. One false killer whale sample had exceptionally high levels of DDT compounds (1700 mg kg −1 DDE, 120 mg kg −1 DDT and 40 mg kg −1 o,p′-DDT) and Toxaphene (89 mg kg −1). PCB levels in the cetaceans were highest in the false and killer whales (22 to 46 mg kg −1 GM), and lowest in the Risso's dolphin (1.7 mg kg −1). Levels of DDE in the British Columbia harbour porpoises were 6.0 mg kg −1, and probably reflect the accumulation of global background levels of DDE.

SUMMER FEEDING HABITS OF DALL'S PORPOISE, PHOCOENOIDES DALLI, IN THE SOUTHERN SEA OF OKHOTSK

Stomach contents were analyzed from 85 Dall's porpoises, Phocoenoides dalli, taken in the southern Sea of Okhotsk during the summer of 1988. Thirteen species of fish and five species of cephalopods were identified. Fishes comprising 9 families were predominant and made up 79.9% of the total number of prey, with an overall occurrence in the stomachs of 100%. Three families of cephalopods made up 20.1% of the total number of prey ingested, with an overall occurrence of 74%. The Japanese pilchard, Sardinops sagax melanostictus, was the primary prey and represented 72.0% of the total prey in 97.3% of the stomachs examined. The gonatid squid, Berryteuthis magister, ranked second and made up 16.9% of the total prey in 61.6% of the stomachs. Walleye pollock, Theragra chalcogramma, was the third most abundant prey item consumed and represented 6.0% of the total, with an occurrence of 53.4%. Composition of the sample by the calculated total mass and caloric intake supports the numeric findings on the relative importance of the three major prey species to the DaIl's porpoise diet. The Japanese pilchard was clearly dominant using these indices and constituted 65.4% of the total mass and 80.7% of the caloric intake. Berryteuthis magister made up 26.8% of the mass and 15.5% of the caloric intake. Walleye pollock contributed the least to the diet with 7.9% of the total mass and 3.8% of the caloric intake. Volumetric data indicate that porpoises are feeding heavily on Japanese pilchard throughout the daylight hours. Berryteuthis magister was probably fed upon in the late night or early morning hours. Occurrence of both Dall's porpoise and Japanese pilchard in the coastal waters of the southern Sea of Okhotsk is seasonal. The summer movements of Dall's porpoise into this region may be related to the seasonal migration and abundance of the Japanese pilchard.

Enantiomeric ratios of α-hexachlorocyclohexane in blubber of small cetaceans

The enantiomeric ratio of α-hexachlorocyclohexane (α-HCH) in blubber of 10 species of adult male small cetaceans has been determined by means of capillary gas chromatography using β-cyclodextrin as a chiral stationary phase. The enantiomeric ratio of (+)-α-HCH/(−)-α-HCH ranged from 1.6 to 2.8, showing diverse values. Moreover, even in the same species, the ratios varied between animals collected from different localities. Dall's porpoise ( Phocoenoides dalli) collected from the Bering Sea had enantiomeric ratios of 2.0–2.1 and others, from the North Pacific and Japan Sea, exhibited ratios ranging from 1.6–1.9. The ratios of β-HCH concentration to total HCH concentration (β-HCH/∑HCH) was linearly related to the enantiomeric ratios of α-HCH ( r = −0.46, p < 0.005). This may indicate that the metabolic capacity to degrade HCH isomers among cetaceans can be evaluated in terms of degradation of the (+)-α-HCH enantiomer. Present enantiomeric ratios were compared with earlier observations on various environmental samples and unbalanced degradation of (+)- and (−)-α-HCH enantiomers was suggested in small cetaceans.