Abstract The Kanto Basin developed, starting c. 3 Ma, influenced by the subduction of the Philippine Sea plate and the Pacific plate. Sediments in this basin have influenced the geomorphology of the Kanto region. In the Boso Peninsula, located at the eastern edge of the Kanto Basin, uplift continues in what is called the Kashima–Boso uplift zone. Although the development of this uplift after the Late Pleistocene is well understood, there are few data from the Early to Middle Pleistocene. In this study, we investigated the offshore shelf area east of the Boso Peninsula using a high-resolution seismic reflection survey, and report new information on the geological structure and uplift processes in the area from the Early to Middle Pleistocene. We identified the Kujukuri-oki anticline and the Kujukuri-oki normal fault zone. The Kujukuri-oki anticline, more than 47 km long, is north–south striking and deforms the Kujukuri-oki Group. There are numerous normal faults with displacements of less than tens of metres spread widely in the survey area (Kujukuri-oki normal fault zone). These findings reveal that the Kujukuri-oki anticline uplifted during the end of the Early Pleistocene and attenuated during the Middle Pleistocene. This anticline comprised the axis of the Kashima–Boso uplift zone at the Boso Peninsula from the Early to Middle Pleistocene and the Boso Peninsula is located at the western limb of this anticline.