Phenyl Acetyl Research Articles

Synthesis, characterization, DFT calculations and biological evaluation of azo dye ligand containing 1,3-dimethylxanthine and its Co(II), Cu(II) and Zn(II) complexes

ABSTRACT. The azo dye of 1,3-dimethylxanthine, namely 8-[(4-acetyl phenyl azo)]-1,3-dimethylxanthine (APAX), was prepared in two steps. The first step included the formation of the diazonium salt by reacting 4-aminoacetophenone with sodium nitrite NaNO2 under acidic conditions and completing the diazotization process while maintaining a low temperature below 5 °C. The resulting diazonium salt was reacted with the 1,3-dimethylxanthine compound and obtained on the azo dye, which was characterized by UV-Vis, FT-IR, 1H-NMR spectroscopies and elemental analysis. DFT calculations were performed to obtain optimized geometry of the ligand. These calculations were performed using three functionals and 6-311++ G (d,p) basis and allow to determinate bond length, bond angles, dihedral and HOMO and LUMO energies. The APAX ligand exhibited considerable color changes when it mixed with solution of cobalt, copper and zinc ions, therefore, it is considered a probe to detect these ions. Metallic complexes of APAX ligand were prepared by interacting aqueous solutions of the metallic salts of Co(II), Cu(II) and Zn(II) with APAX ligand under a molar ratio equal to 2:1 (APAX ligand : metal). The APAX dye and the complexes showed exciting biological activity against E. coli, Staph. aureus and Aspergillus. The APAX dye was pH sensitive and exhibited interesting photochromic behavior.
 
 KEY WORDS: 1,3-Dimethylxanthine, Dye, Complexes, pH sensitive, Probe, Bacteria, DFT calculations
 
 Bull. Chem. Soc. Ethiop. 2023, 37(2), 347-356. 
 DOI: https://dx.doi.org/10.4314/bcse.v37i2.8

Synthesis, Identification and Biological Activities Of a New Series of Heterocyclic Amide

A new series of heterocyclic amides have been prepared (2a-k) from the reaction of phenyl acetyl chloride with a series of heterocyclic amines containing a 1,3,4- dithiazaol unit. The structures of these new compounds were confirmed on the basis of IR, and one of them by 1H-NMR, 13C-NMR. The synthesized products were tested for antimicrobial activity against a variety of test organisms: Staphylococcus aureus(+ve), Escherichia coli(-ve), Enterobactria Cloacae (-ve) & Klebsiella(-ve).

Facile synthesis, crystal structure, biological evaluation, and molecular modeling studies of N-((4-acetyl phenyl) carbamothioyl) pivalamide as the multitarget-directed ligand.

The crystal structure of N-((4-acetylphenyl)carbamothioyl)pivalamide (3) was synthesized by inert refluxing pivaloyl isothiocyanate (2) and 4-aminoacetophenone in dry acetone. The spectroscopic characterization (1H-NMR, 13CNMR, FT-IR) and single crystal assays determined the structure of synthesized compound (3). Systematic experimental and theoretical studies were conducted to determine the molecular characteristics of the synthesized crystal. The biological examination of (3) was conducted against a variety of enzymes i.e., acetyl cholinesterase (AChE), butyl cholinesterase (BChE), alpha amylase, and urease enzyme were evaluated. The crystal exhibited approximately 85% enzyme inhibition activity against BChE and AChE, but only 73.8 % and 57.9% inhibition activity against urease and alpha amylase was observed respectively. The theoretical calculations were conducted using density functional theory studies (DFTs) with the 6–31G (d, p) basis set and B3LYP functional correlation. The Frontier molecular orbital analysis revealed that the HOMO/LUMO energy gap was smaller, which corresponds to the molecule’s reactivity. In terms of reactivity, the chemical softness value was found to be in good agreement with experimental values. In Crystal structure analysis, the intramolecular N—H•••O hydrogen bond generates a S 6) ring motif and N—H•••O interactions exist in crystal structure between the centroids of neighboring parallel aromatic (C4-C9) rings with a centroid to centroid distance of 3.9766 (7)Å. These intermolecular interactions were useful in structural stabilization. The Hirshfeld surfaces and their related two-dimensional fingerprint plots were used for thorough investigation of intermolecular interactions. According to Hirshfeld surface analysis of the crystal structure the most substantial contributions to the crystal packing are from H ••• O and H ••• N/N ••• H interactions. Molecular docking studies were conducted to evaluate the binding orientation of synthesized crystal with multiple targets. The compound exhibited stronger interactions with AChE and BChE with binding energies of -7.5 and -7.6 kcal/mol, respectively. On the basis of in-vitro and in-silico findings, it is deduced that N-((4-acetylphenyl)carbamothioyl)pivalamide 3) possesses reactive and potent multiple target inhibitory properties.

Crystal structures of three transition metal complexes with salicylaldehyde-4-hydroxy phenylacetyl acylhydrazone and their interactions with CT-DNA and BSA

A salicylaldehyde-4-hydroxy phenyl acetyl acyl hydrazone (C15H14N2O3, HL) and its three complexes ([CuLPy]NO3 (1), [CuLCl(EtOH)] (2) and [CoL2]NO3·EtOH, (3)) were synthesized. The single crystal X-ray diffraction results of 1, 2 and 3 showed that the coordination atoms (N and O atoms) in the complexes exhibited four-coordinated pyramidal, five-coordinated and six-coordinated octahedral geometries centered on the metal ions, respectively. TG-DTG techniques illustrated that the maximum thermal decomposition peak temperatures of these compounds were all higher than 200 ℃, exhibiting strong thermal stabilities. All four compounds, HL, 1, 2 and 3, quenched the intrinsic fluorescence of BSA by means of static processes, as investigated by fluorescence spectra, which suggested that these compounds could effectively combine with BSA to form non-fluorescent ground state complexes. The UV–vis spectra showed that HL, 1, 2 and 3 effectively combined with CT-DNA through intercalative modes. Microcalorimetry tests indicated that the interaction processes of HL, 1 and 2 with BSA and CT-DNA were all spontaneously exothermic and entropy reducing, and each of the interaction durations was within 50 min. The interactions of the four compounds with BSA and CTDNA were also simulated by the molecular docking method, showing that the four compounds had hydrogen bonds and hydrophobic interactions with BSA and CT-DNA. The antibacterial activities of HL, 1, 2 and 3 against S. aureus, P. aeruginosa, B. subtilis and E. coli were determined, revealing that 1 and 2 exhibit stronger inhibition activities against P. aeruginosa than gentamicin sulfate. Complexes 1 and 2 are potential alternative drugs to gentamicin sulfate in the future.

Synthesis, characterization, computational study, and screening of novel 1-phenyl-4-(2-phenylacetyl)-thiosemicarbazide derivatives for their antioxidant and antimicrobial activities

Condensation reaction of phenyl acetyl isothiocyanate (1) and phenyl hydrazine was performed to obtain 1-phenyl-4-(2-phenylacetyl) thiosemicarbazide (2). Compound (2) was utilized as starting material for synthesis of different fused 1,2,4-triazole compounds. Reaction of (2) with hydrazine hydrate, anthranilic acid and ethyl glycinate under microwave irradiation and traditional thermal conditions afforded 1,2,4-traizol derivatives (4, 5 and 6) respectively. On the other hand, cyclization reaction of compound (2) under microwave irradiation gave 1,2,4-triazole-3-thione derivative (3). The microwave assessed reaction produced higher yield reaction and purity than the traditional thermal reaction. The chemical structures of the synthesized compounds were elucidated using elemental analysis, FTIR, 1H NMR, and GC/MS spectroscopy. The synthesized compounds (2–7) were evaluated for their antimicrobial and antioxidant potentials. Density functional theory (DFT) was evaluated at B3LYP/3-21G level to study the electronic and geometric characteristics of (4–6) compounds including the optimized structures, relative binding energies, atomic charges, position of HOMO and LUMO of the molecules.

Synthesis And Antibacterial Evaluation Of Some New 1, 5- Benzooxazepines Derivatives

Ten new chalcones were prepared in three steps, firstly phthalic anhydride reacted with 4- amino acetophenone to produce 2-(4-acetyl phenyl carbamoyl) benzoic acid (M1), secondly [2-(4-acetyl phenyl carbamoyl) benzoic acid (M1)] suffers from the loss of a water molecule via reaction with (anhydrous sodium acetate and acetic anhydride) to produce 2-(4-acetyl phenyl) isoindoline-1,3-dione (M2), thirdly (M2) condensed with various substituted benzaldehydes affording chalcones (M3- M12) were reacted with 2-aminophenol to produce 1, 5- benzooxazepines compounds (M13- M22). The prepared compounds were characterized by determination of melting point, FT-IR and some of the prepared compounds have been characterized by (1H-NMR and 13C-NMR) techniques, the antibacterial activity of some of the compounds have also been evaluated.

Spectroscopic Investigations and DFT Studies of Synthesized 4- Acetamidophenyl 3,4,5-Trimethoxybenzoate and 4-acetyl phenyl 3,4,5- trimethoxybenzoate a Novel Conjugate of Gallic Acid

Gallic acid is abundantly found in amla (Phyllanthus emblica), a deciduous of the family phyllanthaceae. Gallic acid, the major constituent of the plant, was methylated to 3,4,5 trimethoxy gallic acid, which then underwent steglich esterification first with paracetamol and then with 4-hydroxy acetophenone to yield 4-acetamidophenyl 3,4,5-trimethoxybenzoate and 4-acetyl phenyl 3,4,5-trimethoxybenzoate “respectively”. 1H NMR, 13C NMR, UV, FT-IR and mass spectroscopy were used to characterize the synthesized compounds. Density functional theory (B3YLP) using a 6-31G (d,p) basis set has been used for quantum chemical calculations. AIM (Atom in a molecule) approach depicted weak molecular interactions within the molecules, whereas the reactive site and reactivity within the molecule were examined by global and local reactivity descriptors. The HOMO and LUMO energies and frontier orbital energy gaps were calculated by time dependent DFT approach using the IEFPCM model. A small value for the HOMO-LUMO energy gap indicated that easier charge transfer occurs within compound 4. The nucleophilic and electrophilic reactivity was determined by MEP (molecular electrostatic potential) experiment. Polarizability, dipole moment, and first hyperpolarizability values were calculated to depict the NLO (nonlinear optical) property of both the synthesized compounds. The antimicrobial activity was also carried out and broad-spectrum antibacterial activity against several strains of bacteria and certain unicellular fungi were exhibited by synthesized compound 3.

Synthesis and Antibacterial Evaluation of some New Pyrazole Derivatives

Ten new chalcones were prepared in three steps, firstly phthalic anhydride reacted with 4- amino acetophenone to produce 2-(4-acetyl phenyl carbamoyl) benzoic acid (A1), secondly [2-(4-acetyl phenyl carbamoyl) benzoic acid (A1)] suffers from the loss of a water molecule via reaction with (anhydrous sodium acetate and acetic anhydride) to produce 2-(4-acetyl phenyl) isoindoline-1,3-dione (A2), thirdly this compound condensed with various substituted benzaldehydes affording chalcones (A3- A12) were reacted with hydrazine hydrate to produce pyrazole compounds (A13- A22). The prepared compounds were characterized by determination of melting point, FT-IR and some of the prepared compounds have been characterized by (1 H-NMR and 13C-NMR) techniques.

Microwave Assisted Synthesis Characterization and Study of Some Novel Chalcones Compounds Derived From Mefenamic Acid

New chalcones compounds were synthesis by reaction mefenamyl chloride with P-aminoacetophenone to from N–(4-acetyl phenyl)-2-(2/3- dimethyl phenyl) amino benzamide(A). The chalcones produced by Claisen – Schmidt condensation of compound A with substituted benzaldehydes [(2,4-dihydroxy(1), 3-NH2(2) and 4- OMe (3)] in ethanollic KOH solvent by using microwave irradiation. The new compounds were characterized by M.P, TLC, CHN, FT-IR, 1HNMR and 13CNMR. The biological screening data of the synthesized compounds were also presented. The theoretical study by using AM1 semi-empirical method emphasizes these compounds exists as trans configuration.

Standardization and xanthine oxidase inhibitory potential of Zanthoxylum armatum fruits

Ethnopharmacological relevanceTejovati (Zanthoxylum armatum DC; Family- Rutaceae) popularly known as toothache tree is widely distributed in sub-tropical Himalaya region. Traditionally, The Southeast Asian population of Indo-Nepal origin uses it to treat asthma, gout, pain, and inflammation. The Ayurvedic action of the plant includes the balancing of Vata-Kapha in the body. Which lead to various ailments related to the circulation of blood and water, digestion, immunity, and skin. Therefore, in-vitro xanthine oxidase (XO) inhibition potential of the extract could be worth to explore prospect in the prevention/treatment of gouty affections of the joints and other diseases. Aim of studyAnti-inflammatory and antioxidant potential of Z. armatum fruit (ZAF) has been reported. To date, no scientific study to validate the claim for gout treatment/management has been attempted so far. The present study deals with the xanthine oxidase inhibitory potential of a various extract of ZAF and marker-based high-performance liquid chromatography (HPLC) standardization of most active fraction. Materials and methodsLiquid-liquid partioning of crude methanol extract of the ZAF followed by repeated column chromatography of most active fraction has resulted in the isolation of seven compounds. Five distinct groups of compounds were isolated, purified, and identified. We have investigated the therapeutic action of ZAF in the management of gout through in-vitro assay of XO, a key enzyme involved in gout pathogenesis. ResultsPhytochemical investigation of ZAF has resulted in the isolation of seven compounds of diverse nature. It is noteworthy to mention that out of seven, five compounds have shown the xanthine oxidase inhibitory action. The ethyl acetate fraction was most potent to inhibit XO. The XO inhibitory activity (IC50 values) of isolated marker chemical was ranging from 5.62 to 41.21 µM. Three compounds viz. acetyl phenyl acetate (ZA-2), prudomestin (ZA-6), and tambulin (ZA-7) showed the most potent XO inhibitory activity (IC50 ≈ 6 µM) comparable with a positive control (Allopurinol, IC50, 3.38 µM). This is the first validated HPLC-PDA method for simultaneous analysis and accurate quantification of seven compounds (phenolic acid, acetyl phenyl acetate, xylopyranoside, diphenyl ether and three flavones) in ZAF as well as their distribution in other tissues of the plant. ConclusionMost potent three chemicals (ZA-2, 6 and 7) could be considered as bioactive to ensure the robust quality of the enriched fraction of ZAF with defined XO inhibition potential. Therefore, either single purified component or their enriched fraction could be a better choice for the management of gout than the crude extract of ZAF. Developed HPLC method is suitable for quality assurance analysis and process control of ZAF derived product intended for gout management. XO inhibitory potential exhibited by the characterized compounds validate the traditional use of this ZAF for the treatment of gout. Further, a detailed study is required to assess the effect of ZAF chemicals on serum uric acid and mechanism of XO inhibition.

Mediterranean Diet Score: Associations with Metabolic Products of the Intestinal Microbiome, Carotid Plaque Burden, and Renal Function.

Metabolic products of the intestinal microbiome such as trimethylamine N-oxide (TMAO) that accumulate in renal failure (gut-derived uremic toxins, GDUTs) affect atherosclerosis and increase cardiovascular risk. We hypothesized that patients on a Mediterranean diet and those consuming lower amounts of dietary precursors would have lower levels of GDUTs. Patients attending vascular prevention clinics completed a Harvard Food Frequency Questionnaire (FFQ) and had plasma levels of TMAO, p-cresylsulfate, hippuric acid, indoxyl sulfate, p-cresyl glucuronide, phenyl acetyl glutamine, and phenyl sulfate measured by ultra-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry. Carotid plaque burden was measured by ultrasound; CKD-Epi equations were used to estimate the glomerular filtration rate. In total, 276 patients completed the study. Even moderate renal function significantly increased plasma GDUTs, which were significantly associated with higher carotid plaque burden. There was no significant difference in plasma levels of any GDUT associated with a Mediterranean diet score or with intake of dietary precursors. In omnivorous patients with vascular disease, the intake of dietary precursors of intestinal metabolites or adherence to a Mediterranean diet did not change plasma GDUT. Approaches other than diet, such as probiotics and repopulation of the intestinal microbiome, may be required to mitigate the adverse effects of GDUTs.

New chemical constituents from the fruits of Zanthoxylum armatum and its in vitro anti-inflammatory profile

Chemical investigations on the fruits of Zanthoxylum armatum Roxb. (Rutaceae) led to the isolation of two new constituents characterised as 2α-methyl-2β-ethylene-3β-isopropyl-cyclohexan-1β, 3α-diol (1) and phenol-O-β-D-arabinopyranosyl-4′-(3″, 7″, 11″, 15″-tetramethyl)-hexadecan-1″-oate (2) along with known compounds m-methoxy palmityloxy benzene (3), acetyl phenyl acetate (4), linoleiyl-O-α-D-xylopyranoside (5), m-hydroxyphenoxy benzene (6) and palmitic acid (7). The chemical structures were established with the help of physical, chemical and spectroscopic methods. The anti-inflammatory potential of isolated compounds 1 and 2 was evaluated using in vitro target-based anti-inflammatory activity in LPS-stimulated primary peritoneal macrophages isolated from mice. Production of pro-inflammatory cytokines (TNF-α and IL-6) was significantly inhibited by the treatment of isolated compounds 1 and 2 in a dose-dependent manner.

1H NMR and MVA metabolomic profiles of urines from piglets fed with boluses contaminated with a mixture of five mycotoxins

Metabolic profile of urine from piglets administered with single boluses contaminated with mycotoxin mixture (deoxynivalenol, aflatoxin B1, fumonisin B1, zearalenone, and ochratoxin A) were studied by 1H NMR spectroscopy and chemometrics (PCA, PLS-DA, and OPLS-DA). The mycotoxin levels were close to the established maximum and guidance levels for animal feed (2003/100/EC and 2006/576/EC). Urine samples were obtained from four groups of four piglets before (control, C) or within 24h (treated, T) after receiving a contaminated boluses with increasing doses of mycotoxins (boluses 1–4). For the two highest dose groups, the urines were collected also after one week of wash out (W). For the two lowest doses groups no significant differences between the C and T samples were observed. By contrast, for the two highest doses groups the T urines separated from the controls for a higher relative content of creatinine, p-cresol glucuronide and phenyl acetyl glycine and lower concentration of betaine and TMAO. Interestingly, a similar profile was found for both W and T urines suggesting, at least for the highest doses used, serious alteration after a single bolus of mycotoxin mixture.

Nitroxide-Mediated Radical Polymerization of Styrene Initiated from the Surface of Titanium Oxide Nanoparticles

Titanium dioxide (TiO 2 ) nanoparticles, with an average size of about 45 nm, were encapsulated by polystyrene using in situ nitroxide mediated radical polymerization in the presence of 3-aminopropyl triethoxy silane as a coupling agent and 2, 2, 6, 6-tetramethylpiperidinyl-1-oxy as a initiator. First, the initiator for nitroxide mediated radical polymerization was covalently bonded onto the surface of Titanium dioxide nanoparticles through our novel method. For this purpose, the surface of TiO 2 nanoparticle was treated with 3-aminopropyl triethoxy silane, a silane coupling agent, and then these functionalized nanoparticles was reacted with ± -chloro phenyl acetyl chloride. The chlorine groups were converted to nitroxide mediated groups by coupling with 1-hydroxy-2, 2, 6, 6-tetramethyl piperidine. These modified TiO 2 nanoparticles were then dispersed in styrene monomers to carry out the in situ free radical polymerization.

ChemInform Abstract: A Facile Access to 4‐Substituted‐2‐naphthols via a Tandem Friedel—Crafts Reaction: A β‐Chlorovinyl Ketone Pathway.

AbstractThe transformation of phenyl acetyl chlorides and asymmetrically disubstituted alkynes results in the regioselective formation of β‐chlorovinyl ketone intermediates offering access to 3,4‐disubstituted‐2‐naphthols.

English

A mangrove Penicillium producing red pigment was cultured in an optimized medium that was designed by the authors previously and used in this study. The purpose of this study was to identify the pigment and also to study the effect of bio elements on pigment production. Pigment from the medium was efficiently extracted using chloroform, ethyl acetate and n-butanol. Most of the red pigment was extracted into ethyl acetate and further purified by preparative thin layer chromatography. From 1 H and 13 C NMR data supported by electronic imaging mass spectrometry, structure of the compound was elucidated as 2-(4-acetyl phenyl) acetic acid. The yield of pigment produced was studied with respect to various salts and bio elements. Salts at high concentrations (sodium chloride, ammonium sulfate, and sodium nitrite) had a drastic effect on pigment yield because most of the pigment remained adhered to the mycelium instead of diffusing into the medium. Also, when bio elements were supplemented to the medium; calcium, iron, and zinc enhanced pigment yield whereas; potassium, magnesium, copper and manganese did not have significant impact on pigment production. Lead had a drastic negative effect on the pigment yield. Therefore, this study proves that salts and bio elements play a major role in the production of various metabolites from mangrove fungi. Keywords: Penicillium , 2-(4-acetyl phenyl) acetic acid, bio elements, salts, soluble pigment. African Journal of Biotechnology , Vol 13(26) 2668-2674

Synthesis, Characterization and Biological Activity of New Mefenamic acid - Oxoazetidine Derivatives

Several new oxoazetidine derivatives based on mefenamic acid were synthesized via a four steps method in good yields.1st step was prepared 2-[2-chloro-N-(2,3- dimethylphenyl)acetamido]benzoic acid (2) by condensation of mefenamic acid with chloroacetyl chloride, which on amination with hydrazine hydrate in ethanol to give 2-[N- (2,3-dimethylphenyl)-2-hydrazinylacetamido] benzoic acid (3) in good yields(2nd step).Compound 3, on condensation with various aldehydes afforded a series of Schiff bases 2-[N- (2,3-dimethylphenyl)-2-(2-arylhydrazinyl)acetamido]benzoic acid (4a-f) (3rd step).A series of oxoazetidine have been synthesized by cyclocondensation of various Schiffm bases (4a-f) with chloroacetyl chloride or phenyl acetyl chloride in presence of triethyl amine to yield compounds (5a-f) and (6a-f), respectively (4th step). Purified compounds were characterized by mp, TLC, UV, FT-IR and elemental analysis.The synthesized compounds (5a-f) were screened for their antibacterial activities against three species of bacteria. They were found to exhibit good antibacterial activity.

Poly(arylene ether)s with Pendent 3-Iodophenyl Sulfonyl Groups: Synthesis, Characterization, and Modification

A series of poly(arylene ether)s carrying truly pendent functional groups were prepared by the meta activated nucleophilic aromatic substitution (NAS) polycondensation reaction of a new aryl difluoride monomer, 3,5-difluoro-1-((3-iodophenyl)sulfonyl)benzene, followed by Suzuki–Miyaura and Heck cross-coupling reactions. The synthesis of 3,5-difluoro-1-((3-iodophenyl)sulfonyl)benzene was achieved via the one-step reaction of 3,5-difluorodiphenyl sulfone with N-iodosuccinimide. Model reactions and NMR data indicated that the carbon–iodine functionality was stable to NAS conditions, thus allowing the synthesis of the desired iodo functional poly(arylene ether sulfone), I-PAES. The iodo moiety was then subjected to palladium-catalyzed cross-coupling reactions with phenyl, naphthyl, and 4-acetylphenyl boronic acids as well as styrene in order to prepare the corresponding phenyl, naphthyl, 4-acetyl phenyl, and 2-phenylvinyl analogues. The polymers were characterized via NMR spectroscopy, size exclusion chromatography, thermogravimetric analysis, and differential scanning calorimetry. The polymers exhibited moderate thermal stability in air, with 5% weight loss temperatures ranging from 404 to 482 °C, while the glass transition temperatures ranged from 131 to 165 °C.

Synthesis of 3-Aryl Coumarin Derivatives Using Ultrasound

Coumarins occupy an important place in the realm of natural products and synthetic organic chemistry. A fast and highly efficient green method for synthesizing 3-aryl coumarin derivatives from salicylaldehyde and phenyl acetyl chloride in the presence of tetrahydrofuran and K2CO3 using ultrasound irradiation is reported. Ultrasound assisted reactions have resulted in better yields and faster reaction time of the desired products than when prepared under conventional conditions. The resulting coumarin derivatives were characterized by IR spectrum.

Synthesis of Poly (methyl methacrylate)/Zinc Oxide Nanocomposite with Core-Shell Morphology by Atom Transfer Radical Polymerization

A method to prepare zinc oxide (ZnO) nanoparticles with a covalently bonded poly(methyl methacrylate) (PMMA) shell by surface initiated atom transfer radical polymerization (ATRP) was reported. First, the initiator for ATRP was covalently bonded onto the surface of zinc oxide nanoparticles through our novel method. Firstly, the surface of ZnO nanoparticle was treated with 3-aminopropyl triethoxysilane, a silane coupling agent, and then this functionalization nanoparticle was reacted with α-chloro phenyl acetyl chloride to prepare atom transfer radical polymerization macroinitiator. The metal-catalyzed radical polymerization of MMA with ZnOmacroinitiator was performed using a copper catalyst system to give the ZnO-based nanoparticles hybrids linking PMMA segments (poly (methyl methacrylate)/zinc oxide nanocomposite). These hybrid nanoparticles had an exceptionally good dispersability in organic solvents and were subjected to detailed characterization using FTIR, TEM and TGA and DSC analyzed.