Phenolic Content Of Fruits Research Articles

Total phenolic content and antioxidant activities in methanol extracts of medicinal herbs from Indo-Gangetic plains of India

The present study investigates total phenolics, flavonoids, and in vitro antioxidant activities in methanol extracts of roots, stems, leaves, and fruits of medicinal herbs (Phyllanthus fraternus G.L. Webster; Bhui-amla and Solanum nigrum L.; Makoi or black nightshade) of Indo-Gangetic plains of India. The results showed that biochemical attributes of methanol extracts of both the tested plants varied significantly with sites (P < 0.05). Contents of total phenolics and flavonoids and antioxidant properties were found highest in fruits followed by leaf, stem, and least in root of P. fraternus and leaves, fruits, stem, and roots of S. nigrum plants. The present investigation revealed that total phenolic content in fruits of P. fraternus ranged from 26.69 to 61.48 mg GAE/g fw and from 8.89 to 24.69 mg GAE/g fw in leaves of S. nigrum plants, and thus, these plant parts can be promoted for pharmaceutical purposes and health benefits. It is also suggested that the individual phenolic compound in the different parts of tested plants should be analyzed to identify their elite population for their mass cultivation, conservation, and sustainable utilization.

Biofortified Beverage with Chlorogenic Acid from Stressed Carrots: Anti-Obesogenic, Antioxidant, and Anti-Inflammatory Properties.

Using wounding stress to increase the bioactive phenolic content in fruits and vegetables offers a promising strategy to enhance their health benefits. When wounded, such phenolics accumulate in plants and can provide antioxidant, anti-inflammatory, and anti-obesogenic properties. This study investigates the potential of using wounding stress-treated carrots biofortified with phenolic compounds as a raw material to extract carrot juice with increased nutraceutical properties. Fresh carrots were subjected to wounding stress via slicing and then stored at 15 °C for 48 h to allow phenolic accumulation. These phenolic-enriched slices were blanched, juiced, and blended with orange juice (75:25 ratio) and 15% (w/v) broccoli sprouts before pasteurization. The pasteurized juice was characterized by its physicochemical attributes and bioactive compound content over 28 days of storage at 4 °C. Additionally, its antioxidant, anti-inflammatory, and anti-obesogenic potentials were assessed using in vitro assays, both pre- and post-storage. The results reveal that juice derived from stressed carrots (SJ) possessed 49%, 83%, and 168% elevated levels of total phenolics, chlorogenic acid, and glucosinolates, respectively, compared to the control juice (CJ) (p < 0.05). Both juices reduced lipid accumulation in 3T3-L1 cells and nitric oxide production in Raw 264.7 cells, without significant differences between them. SJ further displayed a 26.4% increase in cellular antioxidant activity. The juice's bioactive characteristics remained stable throughout storage time. In conclusion, the utilization of juice obtained from stressed carrots in a blend with orange juice and broccoli sprouts offers a promising method to produce a beverage enriched in bioactive compounds and antioxidant potential.

Understanding Environmental and Physiological Factors Affecting the Biology of Diaporthe ilicicola, the Fungus Causing Latent Fruit Rot in Winterberry.

Fruit rot in winterberry is associated with a complex of fungal pathogens. Among them, Diaporthe ilicicola plays a unique role by infecting flowers at bloom, resulting in symptom development in mature fruit. This research aimed to identify at what stage of maturation Ilex fruit can develop disease symptoms and correlate changes in fruit physiology (sugar and phenolic content) and environment (temperature and light intensity) with disease incidence. Correlation data informed in vitro studies testing the ability of putative factors to alter growth of D. ilicicola and select opportunistic fungi within the fruit rot complex: Alternaria alternata, Colletotrichum fioriniae, and Epicoccum nigrum. Results indicated that Ilex fruit do not develop symptoms until 81 to 108 days after inoculation. Temperature and fruit phenolic content were negatively correlated with disease incidence, while fruit sugar concentration and light intensity were positively correlated. In vitro assays revealed that sugar concentration had no effect on the growth of D. ilicicola, but increased light intensity increased hyphal growth and pycnidium formation. Additionally, phenolics extracted from fruit inhibited spore germination in A. alternata, induced secondary conidiation in C. fioriniae, and late season phenolic extracts increased hyphal melanization and pycnidial formation in D. ilicicola. Finally, drops in field temperatures, when replicated in vitro, resulted in a decrease in hyphal growth and spore germination for all fungi. These results suggest that changes in Ilex fruit phenolics during maturation and the increased exposure to light following defoliation may play a role in symptom development by altering D. ilicicola growth within the fruit.

Influence of Genotype and Environment on Fruit Phenolic Composition of Olive

Extra virgin olive oil (EVOO) is renowned for its numerous minor compounds, particularly phenols, which contribute to its health-promoting properties and commercial quality. The phenolic composition of EVOO largely relies on the initial phenolic content of the olive fruits, which represents, therefore, an important trait to be considered in olive breeding programs. However, only limited studies have been conducted so far to compare the relative influence of genotype and environment in the variability of this trait. For that reason, this study aims to assess the influence of genotype (cultivars ‘Arbequina’, ‘Coratina’, ‘Hojiblanca’, ‘Koroneiki’, ‘Martina’, and ‘Picual’) and environment (harvest date, season, and location) on the fruit phenolic content and composition. A wide general variability was obtained in the whole dataset, with total phenols ranging from 6019 to 38,380 μg/g. A stronger effect of the genotype than the environment was observed for total phenolic content (representing 76–90% of total sums of squares) and the different groups of compounds. Notably, significant variations were found among cultivars not only in the total phenol content but also in the percentage of individual compounds within each main phenolic group. Overall, taking into account the entire dataset available, a clear grouping of samples according to genotypes was observed. The results obtained highlight the prominent influence of the genotype (cultivar) over the environment and genotype–environment interactions on the phenolic content and composition in olive fruits; even when considering very contrasting environments as the Mediterranean and subtropical in the present work. These findings suggest the feasibility of breeding selection of new cultivars with distinctive phenolic content and composition.

A Method Based on Digital Image Colorimetry for Determination of Total Phenolic Content in Fruits

A Method Based on Digital Image Colorimetry for Determination of Total Phenolic Content in Fruits

Effect of Altitude and Location on Compositions and Antioxidant Activity of Laurel Cherry (Prunus Laurocerasus L.)

The compositional and antioxidant properties of cherry laurel (Prunus laurocerasus L.) fruit, which grow in two different locations (Trabzon and Rize) and altitudes, were investigated. The results were subjected to principal component analysis to better describe the relations between investigated properties and growing conditions. The results indicated that antioxidant activity, total phenolic content, citric acid, sugars and phenolic compounds were affected by location and altitude. While fruits of Trabzon province have higher composition content than those of Rize province, fruits collected at low altitudes in both regions were found to have superior properties. Total phenolic content in fruits from Trabzon and Rize provinces increased from 21.90 to 23.32 and from 16.84 to 18.91 mg gallic acid equivalent / 100 g dry weight (DW), β-carotene increased from 5.19 to 6.75 and from 4.16 to 5.61 mg / kg DW, and total sugar increased from 81.68 to 131.99 and from 86.44 to 99.58 mg / g DW when altitude decrease from 351 to 49 m and 316 to 14 m, respectively. Chlorogenic acid (1404.46-7358.63 mg / kg DW) and rutin hydrate (1491.05-2712.91 mg / kg DW) were major phenolic compounds in all samples.

The Phenolic Profile of Sweet Cherry Fruits Influenced by Cultivar/Rootstock Combination

The influence of three cultivars ('Carmen', 'Kordia' and 'Regina') grafted on six rootstocks (Mahaleb, 'Colt', 'Oblacinska', 'M × M 14', 'Gisela 5' and 'Gisela 6') on the phenolic profile of sweet cherry fruits was studied during a two-year period. All the individual phenolic compounds were detected using high-pressure liquid chromatography with diode-array detection coupled with mass spectrometry (HPLC-DAD-MSn). In all the examined samples, 54 compounds were identified and divided into five phenolic classes: anthocyanins (4 compounds), flavonols (7), flavanols (11), flavanones (4), and hydroxycinnamic acids (28). Anthocyanins (58%) and hydroxycinnamic acids (31%) showed the greatest amounts in all the examined fruit samples. PCA analysis revealed that among the cultivars, 'Kordia' showed the highest phenolic content. Regarding rootstocks, the lowest values of the most important phenolic compounds were obtained in fruits from trees grafted onto the seedling rootstock Mahaleb. Among the clonal rootstocks, the vigorous 'Colt' and dwarf 'Gisela 5' promoted the highest values of the evaluated phenolic compounds in the cultivars 'Kordia' and 'Carmen', while the dwarf 'Oblacinska' and semi-vigorous 'M × M 14' induced the highest values in the cultivar 'Regina'. By evaluating the influence of cultivars and rootstocks on the phenolic content in fruit, it has been proven that the cultivar has the most significant influence. However, the rootstock also influences the content of a large number of phenolic compounds. The selection of an adequate cultivar/rootstock combination can also be a powerful tool for improving the phenolic content in fruits, and consequently the nutritional value of sweet cherry fruits.

Nutritional status, antioxidant activity and total phenolic content of different fruits and vegetables' peels.

The present study assessed nutritional status, antioxidant activity, and total phenolic content in fruits, i.e., mango (Mangifera indica), apple (Malus domestica), and vegetable, i.e., bottle gourd (Lagenaria siceraria), and ridge gourd (Luffa acutangula) peels. The antioxidant activity and total phenolic content (TPC) were evaluated by using methanol extracts along with 2, 2-diphenyl-1-picrylhydrazyl (DPPH), Folin–Ciocalteu (FC) assay, respectively having Butylated hydroxytoluene (BHT) and Gallic acid (GA) as standard. The TPC and antioxidant activity in the peels ranged from 20 mg GAE/g to 525 mg GAE/g and 15.02% to 75.95%, respectively, which revealed that investigated fruit and vegetable peels are rich source of phytochemical constituents. Bottle gourd peels exhibited the highest value of DPPH compared to the rest of the peels included in the study. Likewise, mango peels had the highest TPC as compared to the rest of the fruit peels. This research showed that the utilization of agricultural wastes should be promoted at commercial level to achieve the nutritional benefit at zero cost and minimize the generation of biological waste.

Combinational effect of chemical treatments on quality of litchi (Litchi chinensis) during storage

Attractive red-coloured pericarp is one of the most important factors in the consumer decision to purchase litchi(Litchi chinensis Sonn.). Red colour of the pericarp turns brown within 2-3 days after harvest which reduces themarketability and commercial value of the fruit drastically and finally incurring huge financial losses to growers.This study was carried out to evaluate the possibility of using combinational application of sodium hypochlorite withsodium chlorite and carnauba wax on the quality of harvested litchi during storage (2016–17). Postharvest treatmentsincluded sequential dipping in sodium hypochlorite (0.2%) (T1), sodium hypochlorite (0.2%) + sodium chlorite (0.05%) (T2), sodium hypochlorite (0.2%) + carnauba wax (10%) (T3) and untreated (control) (T4). Treated fruit werethen packed inplastic punnets and stored at 2°C and 90-95% relative humidity (RH). All the treatments significantlyreduced pericarp browning over control. The most remarkable effect was obtained in fruits treated with sodiumhypochlorite (0.2%) in combination with sodium chlorite (0.05%) as evidenced by delayed anthocyanin degradation,lower polyphenol oxidase activity, fruit decay and weight loss. This treatment also maintained better fruit quality asindicated by higher total soluble solids and phenolic content in fruits, thus can be used as a cost-effective method toreduce pericarp browning and prolong marketable life of litchi up to 25 days.

Host Preference of Bactrocera latifrons (Hendel) (Diptera: Tephritidae) Among Fruits of Solanaceous Plants.

Simple SummaryBactrocera latifrons (Diptera: Tephritidae) is the major pest in economic chili and eggplant of Thailand. Although B. latifrons has many host plant species, it does not unequally prefer each host plant. Results of this study indicated that among seven host plant species of family Solanaceae, Capsicum fruits were preferred by B. latifrons for oviposition over Solanum fruits. Bird chili and banana pepper were the most preferred hosts for B. latifrons. Turkey berry was least preferred by B. latifrons. Results of this study develop knowledge of the specific mechanisms by which host fruit varieties, host fruit species and physiological changes during ripening of host fruit influence B. latifrons’ preference. This is of potential value in field management, quarantine and plant breeding using traditional or transgenic approaches.Host preference of Bactrocera latifrons (Hendel) (Diptera: Tephritidae), major chili and nightshade pest, was studied using seven host plant species of family Solanaceae. Two nightshade species, eggplant, Solanum melongena L. and turkey berry, Solanum torvum Sw.; three pepper and one large chili cultivar of Capsicum annum L., banana pepper, cayenne pepper, noom pepper and duey kai chili; and one small chili cultivar of Capsicum frutescens L., bird chili, were used as tested host plants of B. latifrons for a series of choice test and no-choice test under the laboratory. Results revealed that B. latifrons preferred Capsicum fruits for oviposition rather than Solanum fruits. Bird chili and banana pepper were the most preferred host for B. latifrons, with the highest number of pupae per gram of fruit in no-choice and choice experiment, respectively. Although the best larval performance parameters of B. latifrons were better for eggplant than for other Solanaceous plants, fruit characteristics and total phenolic content in fruit play a major role for host preference of B. latifrons. Turkey berry was least preferred by B. latifrons, with the lowest number of pupae per fruit and it was not oviposited by B. latifrons female fly under the choice situation at all stages of ripeness.

Study on correlation of Antioxidant activities with presence of phenolic and Flavanoid contents in Emblica officinalis and Terminalia chebula

Background: Reactive oxygen species have been known to cause cellular damages that have been implicated to be a causal of major diseases; therefore natural antioxidants have shown a significant impact on human robustness. The present study was carried out to appraise the anti-oxidant activities (In- vitro) and their correlation with presence of Flavanoid and Phenolic content in fruits of Terminalia chebula and Emblica officinalis fruit extracts which is common in herbal Kitchen of India.&#x0D; Methods: The 70% extracts of fruits from Terminalia chebula and Emblica officialis were applied for the study of Anti-oxidant activity. Scavenging radical ability of extracts of these extracts were judged by radical like DPPH.&#x0D; Results: The capability of the extracts of Fruits in exhibiting Antioxidative properties follow the sequence of Terminalia chebula &gt; Emblica officinalis. Since the antioxidant activities were studied in comparison with the standards of Flavanoid, Phenolic and Ascorbic acid. The Flavanoid and Phenolic quantity / amount along with subsequent dilution of Ascorbic acid as in case of DPPH radical assay were assessed as 127.60 ± 0.001 mg/ml, 133.00 ± 0.003 mg/ml for Phenolic Content as Gallic acid equivalent per 100 mg of the Fruit extract.&#x0D; Keywords: Antioxidant, Phenol, Flavanoid, Emblica, Terminalia.

Phenolic Compounds in Mesoamerican Fruits-Characterization, Health Potential and Processing with Innovative Technologies.

Diets rich in phenolic compounds have been associated to reducing the risk of metabolic syndrome and its derived disorders. Fruits are healthy components of the human diet because of their vitamin, mineral, fiber and phenolic profile. However, they have a short shelf-life which is limited by microbiological growth and enzymatic activity. Innovative preservation methods such as high hydrostatic pressure, pulsed electric fields, ultrasound, microwave, cold plasma and ultraviolet light have become popular for the processing of fruits because they can preserve nutritional quality. In this review, the phenolic profile and health potential of 38 Mesoamerican fruits were assessed. Phenolic compounds were classified based on their contribution to the diet as flavonoids, phenolic acids, tannin, lignins and stilbenoids. Due to this composition, fruits showed a wide range of bioactivities which included anti-inflammatory, anti-diabetic, anti-hypertensive and anti-obesity activities, among others. Phenolic content in fruits submitted to innovative food processing technologies depended on parameters such as enzymatic activity, antioxidant capacity, microstructure integrity and cell viability. Innovative technologies could increase phenolic content while assuring microbiological safety by (i) promoting the release of bound phenolic compounds during processing and (ii) inducing the synthesis of phenolic compounds by activation of phenylpropanoid pathway during storage.

Effect of nighttime UV-C irradiation of strawberry plants on phenolic content of fruit: Targeted and non-targeted metabolomic analysis

BACKGROUND: The new approach of using UV-C irradiation followed by a specific dark period to control plant diseases has the potential to become a mainstream treatment in the production of strawberries and other fruits and vegetables. Thus, it is imperative to study the effects of this treatment on fruit quality. METHODS: In this study, short-day ‘Chandler’ strawberry plants grown in growth chamber from bloom to harvest were irradiated twice a week with nighttime UV-C light (253 nm peak value 12.36 J m–2 and the total dose of 50 J m–2 in the range of 240 –260 nm). The effects on the content of key phenolic compounds in mature fruit were studied with targeted analysis and a non-targeted metabolomic approach based on ultra-high performance liquid chromatography high resolution mass spectrometry (UHPLC-HRMS). Analysis of variance-principal component analysis (ANOVA-PCA) was used to associate variance with different experimental factors. RESULTS: Results indicate that the content of anthocyanins, glucosides and glucuronides of quercetin and kaempferol, catechin, pelargonidin rutinoside, and ellagic acid was not affected by UV-C treatment. ANOVA-PCA analysis of the metabolomic dataset showed no significant differences in composition and content of strawberry metabolites between UV-C and control groups; however, differences were observed between harvest times. CONCLUSIONS: Our findings strongly indicate that UV-C treatment of 50 J/m2 twice per week did not affect nutritional values of strawberry fruit. The metabolomic approach combined with ANOVA-PCA used to evaluate strawberry fruit quality after UV-C irradiation proved to be a very powerful tool in providing an overall insight into fruit quality and accurately determines the influence of each experimental factor.

Dissimilar Responses of Ancient Grapevines Recovered in Navarra (Spain) to Arbuscular Mycorrhizal Symbiosis in Terms of Berry Quality

The exploitation of genetic diversity within agricultural plants, including grapevine, is suggested as a valuable tool to cope with the negative impacts of climate change on yield and crop quality. In some winegrowing regions of Europe, there is a renewed interest in knowing the grapevine genetic resources available, focusing on the prospection, recovery, and study of ancient cultivars typical of every zone. Grapevines are naturally associated with arbuscular mycorrhizal fungi (AMF), which provide some benefits to the host plant, although such effects depending on many factors, including variety. Therefore, the aim of this research was to characterize the potential fruit quality of eight old grapevine varieties recovered in Navarre (northeastern of the Iberian Peninsula), associated or not with AMF. The study was carried out on fruit-bearing cuttings grown under controlled conditions (greenhouse). Overall, AMF inoculation reduced bunch and berry mass, as well as phenolic content in fruits. In some varieties, AMF association improved some berry traits by increasing the concentrations of soluble solids and anthocyanins; in others, berry colour, total phenolic and anthocyanin content were diminished in AMF-inoculated plants. The results, therefore, suggest that intraspecific diversity of old grapevines could include different abilities to respond to arbuscular mycorrhizal symbiosis.

Organic Compounds Determined at Different Levels of Ripening of the Pineapple (Ananas comosus L. Merr.) Cv Cayenne in Two Cultivation Systems under Subtropical Conditions

ABSTRACT This article studies the organic compounds of pineapple fruit, during ripening in two cultivation systems (greenhouse and field), in a subtropical region. Total reducing sugars, organic acids, total phenolic content and antioxidant activity were measured during fruit growth (60, 90, 120 and 150 days after full bloom). In addition, leaf ¨D¨ was used for determination of chlorophyll content, pH and malic acid in three moments of the day during the same cycle. The concentrations of reducing sugars, total phenolic content and acidity increased at the end of the growth of the pineapple fruits in both cultivation systems. Field conditions were more favorable for the accumulation of reducing sugars and total phenolic content in fruits.

Hasil dan Kualitas Okra (Abelmoschus esculentus L. Moench.) Merah dan Okra Hijau dengan Jenis Pupuk yang Berbeda

Okra fruit contains phenolic compounds as natural antioxidant. The aim of this research was to analyze the effect of different types of fertilizers on production and quality of two okra varieties. The experiment was conducted in October 2017 to February 2018 at Bogor Agricultural University experimental field at Leuwikopo, Darmaga, Bogor (-6033’49.3”SL, 106043’30.7’’EL). The experiment used nested randomized complete block design with 2 factors and 4 replications. The first factor consisted of two okra varieties with different colour (red and green okra). The second factor was fertilizer types consisted of no fertilizer, organic (cow manure), combination of organic + inorganic, and inorganic fertilizer. The results showed that fertilizer types significantly affected plant height and fruit N content but did not affect fruit production. Red okra had a higher plant height, lower number of leaves and leaf K content than those of the green okra. The interaction effect of both factors was significant on total phenolic content in fruit and P content in leaf. The highest total phenolic content in fruit was showed by red okra with inorganic fertilizer, although it was not significantly different to those of red okra with combination organic + inorganic fertilizer, and also to green okra with organic fertilizer. The highest P content in leaf was found in red okra without fertilizer, however it was not significantly different to red okra with combination organic + inorganic fertilizer, green okra with organic fertilizer, and green okra with inorganic fertilizer.Keywords: inorganic fertilizer, leaf nutrient, organic fertilizer, phenolic compound

Effect of generally regarded as safe (Gras) chemical treatments on the quality of litchi during storage

Pericarp browning of litchi is the most important problem which takes place rapidly after harvest and reduces the commercial acceptability and shelf-life of fruit. To study the combine effect of Generally Regarded as Safe (GRAS) chemicals on the litchi fruits, the combinational treatments with sodium hypochlorite (0.2%) + KMS (0.5%), sodium hypochlorite (0.2%) + KMS (0.6%), sodium hypochlorite (0.2%) + sodium chlorite (0.05%), and sodium hypochlorite (0.2%) + sodium chlorite (0.06%) by the immersion method were given to Shahi litchi fruits. The treated fruits were packed in punnets and stored at 2oC and 90–95% relative humidity. In general, all treatments significantly reduced pericarp browning. The most remarkable effect was obtained with sodium hypochlorite (0.2%) in combination with KMS (0.5%) followed by sodium chlorite (0.05%), which was effective in delaying anthocyanin degradation, polyphenol oxidase activity and weight loss as well as retaining higher amounts of total soluble solids, titratable acidity and phenolic content in fruits. The combinational treatment of sodium hypochlorite and KMS (0.5%) was able to extend shelf life up to 25 days and could be used as cost-effective alternative method to reduce pericarp browning and fruit quality deterioration of litchi during low temperature storage.

Postharvest Attributes of “Washington Navel” Orange as Affected by Preharvest Foliar Application of Calcium Chloride, Potassium Chloride, and Salicylic Acid

ABSTRACTTo evaluate the effects of preharvest foliar application of calcium chloride (CaCl2), potassium chloride (KCl), and salicylic acid (SA) on postharvest quantitative and qualitative attributes and storage life of “Washington navel” orange, an experiment was conducted during two consecutive years. Solutions including CaCl2 (1.5% and 3%), KCl (1.5% and 3%), SA (0.015%, 0.03% and 0.045%), and the combination of CaCl2, KCl, and SA were sprayed on the canopy at the end of the first stage of fruit growth one month after full bloom (fruit diameter was 20 mm) and repeated in late September. The fruits were harvested at commercial maturity stage and were stored at 5°C with 85 ± 5% relative humidity (RH) for 90 days. Quantitative and qualitative characteristics were assessed every month. Preharvest KCl treatment had no significant effect on measured parameters. However, CaCl2 and SA treatments had the most impact on the quantitative and qualitative characteristics, such as firmness and phenolic content of fruit during storage. The SA treatment enhanced fruit appearance and nutritional value compared to the control but could not prevent the weight loss during storage. The results showed that preharvest spray of CaCl2, KCl, SA, and their combination had positive effects on storability of “Washington navel” orange fruit during storage. However, the effect of Ca and SA was more. Application of Ca, K, and SA could be beneficial for orange postharvest instead of mixed elements fertilizers.

Exploration of genetic resources to improve the functional quality of virgin olive oil

Phenolics are claimed to be one of the main responsible for the health benefits associated to the long term consumption of virgin olive oil (VOO). The variability of the phenolic composition was studied in an olive cultivar core collection which holds an extensive genetic diversity. The most abundant VOO phenolics were the secoiridoid derivatives resulting from the hydrolysis of oleuropein, ligstroside and demethyloleuropein present in the fruit, which showed to be on average their main phenolic glucosides. Total phenolic compounds in the fruits and the oils were, in general, significantly and positively strong correlated (r=0.82) with p<0.001. Thus, the fruit phenolic content seems to be the main factor determining the synthesis of VOO phenolics during oil extraction. Data on phenolic composition would be of interest for the selection of optimal parents in olive breeding programs with the aim of obtaining new cultivars with improved functional quality.

Quorum Quenching and Microbial Control through Phenolic Extract of Eugenia Uniflora Fruits.

We describe the characterization of the centesimal composition, mineral and phenolic content of Eugenia uniflora fruit and the determination of the antioxidant, antimicrobial and quorum quenching activities of the pulp phenolic extract. Centesimal composition was determined according to standard methods; trace elements were measured by total reflection X-ray fluorescence spectroscopy. The phenolic compounds were extracted by solid-phase chromatography and quantified by spectrophotometry. Antioxidant activity was determined by using 3 different methods. Antimicrobial activity was evaluated against a panel of foodborne microorganisms and antiquorum sensing activity in Chromobacterium violaceum was performed by measuring inhibition of quorum sensing dependent violacein production. The centesimal composition (per 100 g of pulp) was as follows: protein 3.68 ± 0.21 g, lipids 0.02 ± 0.03 g, carbohydrates 10.31 g and fiber 2.06 g. Trace elements (mg/g of pulp) were determined as: K 0.90, Ca 3.36, Fe 0.60, Zn 0.17, Cl 0.56, Cr 0.06, Ni 0.04, and Cu 0.07. The pulp is a source of phenolic compounds and presents antioxidant activity similar to other berries. The fruit phenolic extract inhibited all tested bacteria. We also found that the fruit phenolic extract at low subinhibitory concentrations inhibited up to 96% of violacein production in C. violaceum, likely due to the fruit's phenolic content. This study shows the contribution of E. uniflora phenolic compounds to the antioxidant, antimicrobial and the newly discovered quorum quenching activity, all of which could be used by the food and pharmaceutical industries to develop new functional products.