Early identification of lameness at all phases of lactation improves milk yield and reduces the incidence of mastitis in the herd. According to the literature we hypothesized that there are associations of electrical conductivity variables of milk flow with lameness in dairy cows. The aim of this study was to determine if blood cortisol and electrical conductivity in the milk flow phases correlate with each other and whether they are related to cow lameness. On one farm, out of 1500 cows, 64 cows with signs of lameness and 56 healthy cows were selected with an average of 2.8 lactations and 60 days in the postpartum period. A local veterinarian who specializes in hoof care treatments identified and scored lameness. During evening milking, the milk flow of all 120 cows was measured using electronic milk flow meters (Lactocorder®, WMB AG, Balgache, Switzerland). Before each milking, two electronic mobile milk flow meters (Lactocorders) were mounted between the milking apparatus and the milking tube to take measurements. We found that the average cortisol concentration in the blood of the studied cows was significantly correlated with the laminitis score. Results of this study indicate that the number of non-lame cows with a milk electrical conductivity level of <6 mS/cm even reached 90.8-92.3% of animals. Milk electrical conductivity indicators ≥ 6 mS/cm were determined in 17.8-29.0% more animals in the group of lame cows compared to the group of non-lame cows. According to our study, we detected that blood cortisol concentration had the strongest positive correlation with milk electrical conductivity indicators. Cows with a greater lameness score had a higher cortisol content and milk conductivity.