Pharmaceutical Use Research Articles

Effects of Different Drying Methods on the Structural Characteristics and Multiple Bioactivities of Rosa roxburghii Tratt Fruit Polysaccharides.

Drying conditions significantly impact the compositions and microstructures of polysaccharides, leading to various effects on their chemical characteristics and bioactivities. The objective of this study was to investigate how different industrial drying techniques, i.e., hot air drying, infrared drying, microwave vacuum drying, and freeze drying, affect the structural properties and biological activities of polysaccharides extracted from Rosa roxburghii Tratt fruit (RRTP). Results revealed that these drying methods significantly altered the extraction yield, molecular weights, monosaccharide ratios, contents of uronic acid and total sugars, gelling properties, particle sizes, thermal stability, and microstructures of RRTPs. However, the monosaccharide composition and functional groups of polysaccharides remained consistent across the different drying techniques. Biological activity assays demonstrated that RRTPs, particularly those processed through microwave vacuum drying (MVD-RRTP), exhibited excellent anti-linoleic acid oxidation, robust anti-glycosylation effects, and significant α-glucosidase inhibition in vitro. The outcomes of this research demonstrate that microwave vacuum drying serves as an effective pre-extraction drying method for RRTPs, enhancing their biological activities. This technique is particularly advantageous for preparing RRTPs intended for use in functional foods and pharmaceuticals, optimizing their health-promoting properties for industrial applications.

Subcritical Water Extraction of Undaria pinnatifida: Comparative Study of the Chemical Properties and Biological Activities across Different Parts.

The subcritical water extraction of Undaria pinnatifida (blade, sporophyll, and root) was evaluated to determine its chemical properties and biological activities. The extraction was conducted at 180 °C and 3 MPa. Root extracts exhibited the highest phenolic content (43.32 ± 0.19 mg phloroglucinol/g) and flavonoid content (31.54 ± 1.63 mg quercetin/g). Sporophyll extracts had the highest total sugar, reducing sugar, and protein content, with 97.35 ± 4.23 mg glucose/g, 56.44 ± 3.10 mg glucose/g, and 84.93 ± 2.82 mg bovine serum albumin (BSA)/g, respectively. The sporophyll contained the highest fucose (41.99%) and mannose (10.37%), whereas the blade had the highest galactose (48.57%) and glucose (17.27%) content. Sporophyll had the highest sulfate content (7.76%). Key compounds included sorbitol, glycerol, L-fucose, and palmitic acid. Root extracts contained the highest antioxidant activity, with IC50 values of 1.51 mg/mL (DPPH), 3.31 mg/mL (ABTS+), and 2.23 mg/mL (FRAP). The root extract exhibited significant α-glucosidase inhibitory activity with an IC50 of 5.07 mg/mL, indicating strong antidiabetic potential. The blade extract showed notable antihypertensive activity with an IC50 of 0.62 mg/mL. Hence, subcritical water extraction to obtain bioactive compounds from U. pinnatifida, supporting their use in functional foods, cosmetics, and pharmaceuticals is highlighted. This study uniquely demonstrates the variation in bioactive compound composition and bioactivities across different parts of U. pinnatifida, providing deeper insights. Significant correlations between chemical properties and biological activities emphasize the use of U. pinnatifida extracts for chronic conditions.

Fabrication of polysulfone-cellulose acetate based TiO2 @SiO2-CuS and Fe3O4 @XG membrane and its application in wastewater treatment

Membrane technology emerged as a preferred method for wastewater treatment due to its efficiency, and low energy consumption, particularly in pharmaceutical industries. In this study, two types of membranes were developed and compared: cellulose acetate with dimethyl sulfoxide, glycerol, and polysulfone with dimethylformamide, and polyvinyl pyrrolidone. These membranes were augmented with nanocomposites, such as Fe3O4 @XG and TiO2 @SiO2-CuS, to enhance performance. Experimental investigations involved doping membranes with nanocomposites at varying concentrations (0.1 wt%, 0.5 wt%, and 1.0 wt%). Atomic force microscopy revealed that increasing nanocomposite content led to smoother surfaces and improved anti-fouling properties. Incorporating nanocomposites reduced the contact angle, with 1.0 wt% PSF/TiO2 @SiO2-CuS exhibiting the lowest angle at 33.134°. Physical property assessments, including density, viscosity, pH, and porosity, were conducted to evaluate the impact of nanocomposites. Under 4 atm pressure, 1.0 wt% CA-TiO2 @SiO2-CuS demonstrated a maximum pure water flux of 3300 ± 50 lit/m2.hr compared to the pure membrane. Furthermore, biodegradability tests indicated a significant weight loss of the nanocomposite membranes, with a 13.89 % increase in CA membrane weight loss compared to a 0.20 % increase in PSF membrane weight loss. The study highlights the potential of these membranes for diverse applications, particularly in wastewater treatment, underscoring their efficiency and suitability for pharmaceutical industry use.

Treatment utilization and non-drug interventions for vasomotor symptoms in breast cancer survivors taking endocrine therapy: Real-world findings from the United States and Europe

ObjectivesVasomotor symptoms induced by endocrine therapy are common in breast cancer survivors and a risk factor for therapy discontinuation and lower quality of life. The REALISE study evaluated the real-world treatment landscape in breast cancer survivors with vasomotor symptoms taking endocrine therapy, including pharmaceuticals, lifestyle changes, and over-the-counter products. Study designSecondary analysis of the Adelphi Vasomotor Disease Specific Programme™, a large cross-sectional point-in-time survey and chart review conducted in the US and five European countries (February–October 2020). Oncologists provided demographic, clinical, and treatment data for adult breast cancer survivors with induced vasomotor symptoms taking endocrine therapy (tamoxifen or aromatase inhibitors); patients voluntarily completed self-report surveys on their symptom severity, concomitant sleep and/or mood symptoms, lifestyle changes, and use of over-the-counter products. Main outcome measuresPatient characteristics; vasomotor symptom severity; use of pharmaceuticals, lifestyle changes, and over-the-counter products (from pre-defined lists); lines of treatment. ResultsOverall, 77 oncologists reported data for 618 breast cancer survivors, of whom 183 (29.6 %) completed self-report forms. Physicians classified 420 (68.0 %) women as experiencing moderate-severe vasomotor symptoms, of whom 66.9 % were receiving treatment. In total, 15.2 % of all breast cancer survivors were prescribed systemic hormone therapy. Venlafaxine (24.7 %), citalopram (16.5 %), and paroxetine (13.6 %) were the most commonly prescribed nonhormonal medications. Lifestyle changes (77.8 %) and over-the-counter products (61.6 %) were common, especially in patients with concomitant sleep and/or mood symptoms. ConclusionsDespite contraindications, a relatively large proportion of treatment-seeking breast cancer survivors with vasomotor symptoms were prescribed systemic hormone therapy. This, combined with high patient-reported use of lifestyle changes and over-the-counter products, suggests a need for symptomatic relief and demand for new nonhormonal alternatives with established safety profiles in this population.

The Continuous and Reversible Transformation of the Polymorphs of an MGAT2 Inhibitor (S-309309) from the Anhydrate to the Hydrate in Response to Relative Humidity.

Polymorphic control is vital for the quality control of pharmaceutical crystals. Here, we investigated the relationship between the hydrate and anhydrate polymorphs of a monoacylglycerol acyltransferase 2 inhibitor (S-309309). Solvent evaporation and slurry conversion revealed two polymorphs, the hydrate and the solvate. The solvate was transformed into the hydrate by heating. X-ray powder diffraction demonstrated that the hydrate was transformed into an anhydrate via an intermediate state when heated. These crystal forms were confirmed under controlled humidity conditions; the presence of the anhydrate, the intermediate hydrate, or the hydrate depended on the relative humidity at 25 °C. The stoichiometry of S-309309 in water in the hydrate form was 4:1. The hydrates and anhydrates exhibited similar crystal structures and stability. The water of hydration in the intermediate hydrate was 0.1-0.15 mol according to the dynamic vapor sorption profile. The stability and dissolution profile of the anhydrate and hydrate showed no significant change due to similar crystal lattices and quick rehydration of the anhydrate. A mechanism for the reversible crystal transformation between the anhydrate and pseudo-polymorphs of the hydrate was discovered. We concluded that S-309309 causes a pseudo-polymorphic transformation; however, this is not a critical issue for pharmaceutical use.

Ethnomedicinal Usage, Phytochemistry and Pharmacological Potential of Solanum surattense Burm. f.

Solanum surattense Burm. f. is a significant member of the Solanaceae family, and the Solanum genus is renowned for its traditional medicinal uses and bioactive potential. This systematic review adheres to PRISMA methodology, analyzing scientific publications between 1753 and 2023 from B-on, Google Scholar, PubMed, Science Direct, and Web of Science, aiming to provide comprehensive and updated information on the distribution, ethnomedicinal uses, chemical constituents, and pharmacological activities of S. surattense, highlighting its potential as a source of herbal drugs. Ethnomedicinally, this species is important to treat skin diseases, piles complications, and toothache. The fruit was found to be the most used part of this plant (25%), together with the whole plant (22%) used to treat different ailments, and its decoction was found to be the most preferable mode of herbal drug preparation. A total of 338 metabolites of various chemical classes were isolated from S. surattense, including 137 (40.53%) terpenoids, 56 (16.56%) phenol derivatives, and 52 (15.38%) lipids. Mixtures of different parts of this plant in water-ethanol have shown in vitro and/or in vivo antioxidant, anti-inflammatory, antimicrobial, anti-tumoral, hepatoprotective, and larvicidal activities. Among the metabolites, 51 were identified and biologically tested, presenting antioxidant, anti-inflammatory, and antitumoral as the most reported activities. Clinical trials in humans made with the whole plant extract showed its efficacy as an anti-asthmatic agent. Mostly steroidal alkaloids and triterpenoids, such as solamargine, solanidine, solasodine, solasonine, tomatidine, xanthosaponin A-B, dioscin, lupeol, and stigmasterol are biologically the most active metabolites with high potency that reflects the new and high potential of this species as a novel source of herbal medicines. More experimental studies and a deeper understanding of this plant must be conducted to ensure its use as a source of raw materials for pharmaceutical use.

Computational Exploration of Atriplex halimus Phytocompounds: A Targeted Approach Toward Inhibiting SARS-CoV-2

The primary aim of this investigation is to shed light on potential targets within the main protease Mpro, spike protein of SARS-CoV-2, and Angiotensine converting enzyme 2 (ACE2) for the exploration of novel inhibitors derived from therapeutic natural compounds originating from Atriplex halimus (AH). Many constituents were discerned within AH extracts through comprehensive gas chromatography/mass spectrometry (GC/MS). Notably, compounds, such as oleocanthal and methyl stearate, exhibited promising attributes across various biological activities, including inhibition of key proteins associated with SARS-CoV-2. Furthermore, absorption, distribution, metabolism, excretion, and toxicity (ADMET) settings were evaluated to explore the potential safety and viability of these compounds for pharmaceutical application, affirming their non-interference with human physiology and thus suggesting their suitability for pharmaceutical use. Catechin, epigallocatechin, and methyl stearate were discovered to be the compounds with the highest affinity to the studied protein showing a great potential to be inhibitors to COVID-19. Consequently, this discovery highlights Atriplex halimus as a promising candidate for utilization in combating the virus.

Removal of indomethacin from water using covalent organic polymers

Abstract In recent years, the massive use of pharmaceuticals has led to the detection of a large number of relevant residues in water. As emerging contaminants, pharmaceuticals residues in the environment pose a major threat to human health and ecosystems. Covalent organic polymers (COPs) possess high stability, uniform pore size, and easy functionalization, which have attracted great attention in water treatment. In this study, a novel covalent organic polymer was successfully studied by a simple synthesis method, combining p-phenylenediamine (Pd) and isophthalaldehyde (Is) via a Schiff-base (referred to PdIs-COP), which was used as an absorbent for the removal of indomethacin from aqueous solutions. The composite was characterized with scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Brunauer-Emmett-Teller (BET) specific surface area and thermogravimetric (TGA) analysis. The effect of changes in the pH values and the adsorption time on the adsorption capacity was also investigated. The results of this study indicated that the absorbent had a high adsorption capacity for indomethacin. The adsorption capacity of PdIs-COP for indomethacin is 92.86 mg g−1 at pH of five and a contact time of 2 min. This shows that PdIs-COP is a novel material with good affinity for pharmaceuticals.

Drug utilization reviews to reduce inappropriate drug use and pharmaceutical costs in inpatients based on diagnosis-related group data.

Irrational pharmacotherapy and increasing pharmacy costs remain major concerns in healthcare systems. Pharmacists are expected to employ diagnosis-related group (DRG) data to analyse inpatient pharmacy utilization. This project aimed to pilot an efficient pharmacist-led programme to analyse factors related to pharmacy expenses, evaluate the rational use of drugs in batch processing, and make further interventions based on DRG data. Patients from the OB25 (caesarean section without comorbidities or complications) DRG were selected in 2018, and the most relevant factors were identified through statistical analysis. Interventions were implemented by sending monthly reports on prescribing data and drug review results for the same DRGs to the department starting in 2019. Pre-post comparisons were conducted to demonstrate changes in pharmacy costs and appropriateness at a tertiary teaching hospital with 2,300 beds in China. A total of 1,110 patients were identified from the OB25 DRG data in 2018. Multivariate linear analysis indicated that the number of items prescribed and wards substantially influenced pharmacy expenditure. Drugs labelled as vital, essential, and non-essential revealed that 46.6% of total pharmacy costs were spent on non-essential drugs, whereas 38.7% were spent on vital drugs. The use of inappropriate pharmaceuticals and drug items was substantially reduced, and the average pharmacy cost after intervention was 336.7 RMB in 2020. The benefit-cost ratio of the programme was 9.86. Interventions based on DRG data are highly efficient and feasible for reducing inpatient pharmacy costs and non-essential drug use.

Linking physiology and demographics, non-ocular pathology and pharmaceutical drug use to standard OCT measures of the inner retina: The PPP project.

To assess the associations between physiology and demographics, non-ocular pathology and pharmaceutical drug use against peri-papillary retinal nerve fibre layer thickness (pRNFL T) and other optical coherence tomography (OCT) inner retinal measures in normal, healthy eyes. A retrospective, cross-sectional study of 705 consecutive participants with bilateral normal, healthy optic nerves and maculae. PRNFL Ts, vertical cup/disc ratio (CDR), cup volume and macular ganglion cell layer-inner plexiform layer (GCL-IPL) Ts were extracted from Cirrus OCT scans, then regressed against predictor variables of participants' physiology and demographics (eye laterality, refraction, intraocular pressure [IOP], age, sex, race/ethnicity, etc.) and non-ocular pathology and pharmaceutical drug use according to the World Health Organisation classifications. Associations were assessed for statistical significance (p < 0.05) and clinical significance (|β| > 95% limits of agreement for repeated measures). A multitude of non-ocular pathology and pharmaceutical drug use were statistically and clinically significantly associated with deviations in standard OCT inner retinal measures, exceeding the magnitude of other factors such as age, IOP and race/ethnicity. Thinner inner retina and larger optic nerve cup measures were linked to use of systemic corticosteroids, sex hormones/modulators, presence of vasomotor/allergic rhinitis and other diseases and drugs (up to -29.3 [-49.88, -8.72] μm pRNFL T, 0.31 [0.07, 0.54] vertical CDR, 0.29 [0.03, 0.54] mm3 cup volume and -10.18 [-16.62, -3.74] μm macular GCL-IPL T; all p < 0.05). Thicker inner retina and smaller optic nerve cup measures were diffusely associated with use of antineoplastic agents, presence of liver or urinary diseases and other diseases and drugs (up to 67.12 [64.92, 69.31] μm pRNFL T, -0.31 [-0.53, -0.09] vertical CDR, -0.06 [-0.11, 0] mm3 cup volume and 28.84 [14.51, 43.17] μm macular GCL-IPL T; all p < 0.05). There are a multitude of systemic diseases and drugs associated with altered OCT inner retinal measures, with magnitudes far exceeding those of other factors such as age, IOP and race/ethnicity. These systemic factors should at least be considered during OCT assessments to ensure precise interpretation of normal versus pathological inner retinal health.

Biochemical and nutritional analysis of seaweed (Iyengaria stellata) and its therapeutic significance, using antioxidant, antimicrobial, and hypoglycemic activities

Seaweeds have gained significant attention for their bioactive compounds and extensive use in pharmaceuticals, nutraceuticals and cosmeceuticals. Therefore, comprehensive analysis was conducted to investigate the nutritional composition, identify and quantify the Phyco-chemicals and to evaluate the pharmacological potential using hypoglycemic, antimicrobial and antioxidant activities of Iyengaria stellata extract. The results of nutritional analysis revealed 39.72±2.60 % total ash, 14.54±0.44 % crude fat, 9.61±0.60 % proteins, 5.29±0.52 % crude fibers, 10.49±1.00 % moisture content, and total energy was 108.26±2.01 Kcal 100 g−1 of dry powder. Approximately, 15 compounds were tentatively detected in ethyl acetate extract with major compounds being Pentadecanoic acid, 14-methyl-, easter (31.62 %), (Z) -9- stearic acid, methyl easter (13.36 %), Elaidic acid, methyl easter (11.46 %), Phytol (6.31 %), n-Hexadecanoic acid (7.47 %), Linolelaidic acid, methyl ester (5.64 %) and 1-octadecyne (5.34 %). Similarly, n-butanol extract contained 16 compounds with primary compounds being Hexadecanoic acid, methyl ester (25.96 %), Elaidic acid methyl ester (21.39 %), Linolelaidic acid methyl ester (11.53 %), Octadecanoic acid, methyl ester (5.89 %), and Hexanedioic acid, dioctyl ester (5.26 %). FT-IR analysis revealed the presence of similar groups of compounds i.e. alcohols, phenols, lipids, carboxylic acids, amines, aromatics, ketones and halogens. Total phenolic contents (TPC) in ethyl acetate and n-butanol extract were measured as 2.982±0.876 mg GAEg−1 and 1.538±0.711 mg GAE g−1, whereas Total Flavonoids contents (TFC) were 2.058 ± 0.121 mg QE g−1 and 1.614 ± 0.643 mg QE g−1, respectively. Ethyl acetate extract exhibited more antioxidant potential with an IC50 (2.59±1.34) compared to n-butanol extract (2.75±1.60). Moreover, n-butanol extract exhibited strong antimicrobial activity. The most susceptible strains were E. coli, S. aureus and P. aeruginosa. In terms of in-vivo hypoglycemic activity, the n-butanol extract proved to be more effective which significantly reduced the blood glucose level up to 226.17±3.43, 201.33±4.63 and 265.17±3.92 at 300, 200 and 100 mg kg−1 doses respectively after 24 h. In conclusion, the seaweed I. stellata exhibited significant potential in terms of its antioxidant, antimicrobial, and hypoglycemic properties.

Continuous flow mechanochemical synthesis of Zr-MOF as effective catalyst for 4-nitrophenol reduction

Nitroaromatic compounds (NACs) are a group of organic chemicals containing one or more nitro functional groups attached to an aromatic ring. These compounds are considered emerging pollutants (EPs) in wastewater due to their potential environmental and human health impacts. These NACs can enter wastewater through various sources, including the production and use of explosives, dyes, pesticides, or pharmaceuticals. 4-nitrophenol (4-NP) is considered a priority pollutant due to its toxicity and potential environmental and health hazards. Thus, its removal is crucial. For that purpose, a novel strategy to carry out Zr-MOFs syntheses has been developed employing I-CHEM technology (Impact Continuous flow Heated Mechanochemistry), an improved wet-milling process. The resulting materials have demonstrated efficacy as catalysts in the reduction of 4-NP towards 4-aminophenol (4-AP), a precursor to paracetamol.Notably, the most promising outcomes were achieved with Zr-MOF prepared through continuous flow mechanochemical processes utilizing Zr-methacrylate clusters as precursor (Zr-MOF/2@RL). 4-NP removal was successfully carried out at room temperature, with an outstanding optimal time of removal of 14 min, 30% faster than with synthesized by conventional methodologies. These findings underscore the innovation brought about by the I-CHEM synthetic approach, where more effective collisions are reached, as well as opening avenues for broader applications of MOFs. The wet-mechanochemical procedure not only demonstrates its novelty, but also presents the potential for scalability under continuous flow conditions, further enhancing the feasibility of large-scale production.

Investigating luminescence signals of pharmaceuticals and dietary supplements for emergency dosimetry

This study investigates the potential use of dietary supplements and pharmaceuticals containing magnesium, calcium or potassium for emergency dosimetry applications using the phenomenon of optically stimulated luminescence (OSL). Signal measurements were carried out using different stimulation wavelengths, and blue light stimulation was found to be the most efficient. More than half of the samples exhibited a measurable OSL signal and relatively high radiation sensitivity compared to other previously measured emergency detectors. Moreover, samples generally demonstrated a linear dose response. Possible causes of their high zero-dose signal were investigated: mechanical processing and UV light excitation. As variability in sensitivity was observed, the test-dose protocol was used during measurements. Furthermore, the study showed a significant loss of OSL signal intensity within 24 h after irradiation, which suggests the necessity for a fading correction. Finally, a dose recovery test was performed to evaluate the materials and the test-dose protocol under realistic conditions. The findings indicate the potential for using pharmaceuticals and dietary supplements in the event of a radiation emergency due to their dosimetric properties and ease of obtaining.

Boron-Catalyzed Michael Reaction of Donor-Acceptor Carboxylic Acid Pairs Enabling Direct Synthesis of 1,5-Dicarboxylic Acids.

A boron-catalyzed Michael reaction using pairs of carboxylic acids was developed. The reaction occurs through dual activation of the two substrates by a boron catalyst, which facilitates boron enolate formation from the donor carboxylic acid with simultaneous activation of the α,β-unsaturated carboxylic acid as the acceptor. α-Aryl and α-alkenyl carboxylic acids were applicable as donors. The versatility and utility of this reaction were demonstrated by the direct use of pharmaceuticals as donor carboxylic acids.

Extraction of polyphenolic compounds from ginkgo leaves using deep eutectic solvents: A potential solution for the sustainable and environmentally friendly isolation of biflavonoids

Ginkgo (Ginkgo biloba L.) is one of the oldest trees on earth and its leaves are traditionally used as herbal medicine. The beneficial effects of ginkgo leaves are attributed to the presence of flavonoids and terpene trilactones. Monomeric flavonoids are frequently studied, but recently much attention has been paid to biflavonoids, dimeric flavonoids, due to their biological activity and potential pharmaceutical use. The development of a suitable and effective extraction protocol focusing on biflavonoids is increasingly in demand. Therefore, in this study, we developed an optimized extraction protocol using deep eutectic solvents (DESs) to extract flavonoids from ginkgo leaves, with special attention to biflavonoids. Initially, 250 DESs were screened using COSMOtherm software to identify those that could strongly solubilize biflavonoids and flavonoids, and the results obtained indicated 15 DESs. Among them, betaine: ethylene glycol (B:EG) 1:2 with 30 % H2O (w/w) showed higher extraction efficiency than the extraction performed with 80 % methanol. In a next step, the extraction with the selected DES was further optimized using a Box-Behnken experimental design. The results obtained showed that the optimum extraction conditions using the selected DES were as follows: Extraction time 45 minutes, the ratio of the initial mass of the shredded leaves to the volume of the DES 20:1 and the temperature 20 °C. Finally, the concentrations of the 5 most abundant biflavonoids in ginkgo leaves (amentoflavone, ginkgetin, isoginkgetin, bilobetin and sciadopitysin) in the extracts were determined using HPLC-DAD. The results showed that B:EG 1:2 with 20 % H2O (w/w) and 30 % H2O (w/w) were suitable for the extraction of all five biflavonoids.

Patterns in health care use and intensity for diagnosed and undiagnosed cognitive impairment in the older australian community: Implications for primary care management

ObjectivesWhile the economic burden imposed by dementia is well-documented, findings are mixed on health care use for those with mild cognitive impairment (MCI). Our objective was to analyse annual, non-hospital medical and pharmaceutical use patterns for older people with undiagnosed MCI and diagnosed dementia, living in the Australian community. MethodsWe analysed panel data from a community sample, the Sydney Memory and Ageing Study (Australia), linked to administrative data on health care use, using two-part models to estimate the probability of using health care and the annual costs incurred by study participants. ResultsPeople with MCI, unaware of their diagnoses, were significantly less likely to incur annual pathology and diagnostic imaging costs relative to cognitively normal individuals. This effect was concentrated in individuals with MCI who had non-amnestic symptoms, lived alone, or had limited carer support. Compared to cognitively normal individuals, people with MCI were predicted to have slightly lower annual costs for broad medical care categories related to the management and diagnosis of cognitive impairment, and people with dementia, substantially higher professional attendances, and pharmaceutical costs. These findings were consistent across estimation models adjusting for attrition over the study. Policy implicationsDiagnosis and symptom management in primary care may enable individuals with MCI to improve their quality of life and prevent more costly future health care use. However, our study found potential gaps in medical service use for people with undiagnosed MCI in the community, especially when they had less support or did not have memory symptoms. Primary care services may need to better diagnose and target these individuals.

Targeted Chemoprofiling and Bioactivity Screening of Willow Bark

AbstractThe chemoprofile of willow bark (Salix cortex) for pharmaceutical use was investigated using a combination of in vitro bioassays and analytical techniques. Salicylates were isolated by means of (semi‐)preparative HPLC, and their structure was elucidated by means of LC‐MS and 1D/2D‐NMR experiments. Bioactivity assays identified key compounds of selected Salix extracts contributing to the overall anti‐inflammatory potential. Finally, these phytochemicals were mapped quantitatively by means of LC‐MS/MS.

Tuning fluorescence of fexofenadine by switching OFF intramolecular photoinduced electron transfer: Application to development of one-step green and high throughput microwell spectrofluorimetric assay for analysis of tablets and plasma.

Fexofenadine (FEX) is a non-sedating antihistamine commonly used for the treatment of allergic conditions such as seasonal rhinitis and chronic idiopathic urticaria. This study describes the tuning "ON" the intrinsic fluorescence of FEX by switching "OFF" its intramolecular photoinduced electron transfer (PET) through the protonation of the piperidinyl nitrogen atom using sulfuric acid. The resulting fluorescence was utilized as a basis for the development of a highly sensitive microwell spectrofluorimetric assay (MW-SFA) for the one-step determination of FEX in pharmaceutical tablets and plasma. The linear range of the assay was 10-500 ng ml-1, and its limit of quantitation was 25.9ng ml-1. The proposed MW-SFA was successfully applied to analyze FEX in pharmaceutical tablets and plasma samples, demonstrating good accuracy and precision. The greenness of the assay was confirmed using three metric assessment tools. In conclusion, the MW-SFA is a straightforward, single-step analysis that requires no experimental adjustments. It offers high sensitivity, efficient sample processing, and environmental sustainability. This assay is highly recommended for pharmaceutical quality control and clinical lab use, particularly for measuring FEX levels.

An Alternative Microbiological Validation for an Online Water Bioburden Analyzer.

The Mettler-Toledo 7000RMS analyzer is a Bio-Fluorescent Particle Counter (BFPC) used to monitor real-time bioburden results from Purified Water (PW). Validation of the analyzer using 13 microorganisms and a low-intensity, fluorescent, polystyrene bead. During the execution of the validation, a laboratory water system that met Purified Water (PW) quality standards was connected to the 7000RMS, and a syringe pump was used to introduce various concentrations of microorganisms and fluorescent polystyrene beads to the analyzer. Samples were collected and tested via the traditional Membrane Filtration (MF) method and the Colony Forming Unit (CFU) plate count results were compared to the Auto-Fluorescent Unit (AFU) of the 7000RMS analyzer. The validation study was designed to follow the guidance in United States Pharmacopeia (USP) Chapter <1223 > (1), European Pharmacopeia (EP) Chapter 5.1.6 (2), PDA Technical Report 33 (3). Concepts and strategies were adapted from EP Chapter 2.6.12 Microbiological Examination of Non-sterile Products: Microbial Enumeration Tests, EP 10.2 (4), European Pharmacopeia Chapter 2.6.1 Sterility, EP 10.2 (5), USP Chapter <61> Microbiological Examination of Non-sterile Products: Microbial Enumeration Tests (6), USP Chapter <71> Sterility Tests (7), Japanese Pharmacopoeia (JP) General Information Chapter G8 Water: Quality Control of Water for Pharmaceutical Use (8). All pre-determined validation acceptance criteria for Accuracy, Specificity, Precision, Limit of Detection (LOD), Limit of Quantitation (LOQ), Linearity, and Range were met. Further, the 7000RMS demonstrated Performance Equivalence to the MF method per USP <1223> but characteristically lacked correlation to the CFU. This validation approach highlights the superior capabilities of the 7000RMS when compared against the traditional compendial MF testing method for PW.

Separation and purification of hyaluronic acid by [formula omitted] nano and micro particles coated with chitosan and silica

Hyaluronic acid (HA), a glycosaminoglycan, is comprised of alternating units of D-glucuronic acid and N-acetylglucosamine. This compound harbors numerous biomedical applications, including its use in pharmaceuticals, wound healing, osteoarthritis treatment, and drug delivery. Its unique composition and exceptional features, such as its high water-absorbing and retaining capacity, have also led to its use in the cosmetics industry. The employment of this biopolymer has given rise to an escalation in the request for its manufacture. The present investigation has explored the correlation between hyaluronic acid and chitosan and silica for the purpose of separation. Consequently, Iron oxide magnetic nano particles and micro particles were produced via co-precipitation method and were layered with chitosan and silica to purify the hyaluronic acid from the fermentation broth that was generated by Streptococcus Zooepidemicus. The size distribution and zeta potentials of the two kinds of particles were gauged with the aid of a dynamic laser light scattering apparatus and zeta potential meter (Malvern, Zeta master) respectively. The confirmation of the chemical structure of the Fe3O4 nanoparticles and Fe3O4 particles conjugated with chitosan and silica was accomplished through the utilization of Fourier Transform Infrared Spectroscopy (FT-IR). Protein contamination was thoroughly characterized by means of sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and Nanodrop 2000/2000c spectrophotometers protein estimation method. The maximum HA adsorption capacity, under optimal pH conditions of 4, was determined to be 87 mg/g, 112 mg/g, 51 mg/g, and 44 mg/g for Fe3O4 −chitosan nanoparticle, Fe3O4 −chitosan micro particle, Fe3O4 −silica microparticle, and Fe3O4 −silica nanoparticle, respectively.