Phagocytosis Activity Of Neutrophils Research Articles

MECHANISMS OF IMMUNOCORRECTIVE ACTION OF PHOTODYNAMIC THERAPY AND EXOSOMES OF MESENCHYMAL STEM CELLS IN PATIENTS WITH CHRONIC WOUNDS OF THE LOWER EXTREMITIES OF DIFFERENT GENESIS

Summary. Introduction. The treaonic wounds of the lower extremities today requires long-term use of antibiotic therapy, which aggravates the course of the wound process. In this regard, the search for new methods of treatment is underway. Low-intensity light exposure of various wavelengths of photodynamic therapy with photosensitizer photosensitizer Dimegin was used as a physical method, and multipotent mesenchymal-stromal cells and their exometabolites in exosomes form were used as a biological method.
 The aim was to study the change of barrier function of oxygen-independent and oxygen-dependent phagocytosis and serum levels of vascular growth factor (VEGF) in patients with chronic wounds of the lower extremities before and after the application of complex light exposure and exosomes.
 Materials and methods. Phagocytosis activity of neutrophils was investigated by assessing chemotaxis, adhesion and endocytosis and by determining reactive oxygen species (ROS) in oxygen-dependent phagocytosis using light and confocal microscopy. VEGF concentration was determined by ELISA.
 Results: after photodynamic therapy using with red light (λ=660nm), activation of oxygen-independent and oxygen-dependent phagocytosis was observed, as well as an increase in the synthesis of vascular growth factor in patients with chronic wounds of the lower extremities. After the application of green light (λ=530nm) and the application of exosomes, the normalization of immune processes was observed, which led to the acceleration of regenerative processes in the wound.
 Conclusions. Thus, after carrying out complex low-intensity light therapy and exosomes, it was possible to completely clear the wounds from necrosis in a short time.

Clinical, Neurophysiological and Immunological Peculiarities of Myasthenia Gravis in Patients with Different Histologic Types of Thymoma

Thymoma is characterized by various clinical manifestations. About half of them are manifested as an asymptomatic course, others occur in a combination with different syndromes. 25% of patients develop compression of tumor blood vessels, nerves and organs of the mediastinum or clinical signs of germination of thymic tumor in other organs. 40% of patients are diagnosed with autoimmune diseases due to dysfunction of the thymus. Generalized myasthenia gravis (75%) is most frequently observed.The objective of the research was to study clinical and immunological peculiarities of myasthenia gravis depending on the size and histological type of thymoma.Materials and methods. The results of clinical and immunological, instrumental and histological examination of 30 patients with myasthenia on the background of thymoma were analyzed. The severity of the disease was assessed using clinical classification of the Myasthenia Gravis Foundation of America (MGFA, 2001).To determine the barrier function of phagocytic cells, phagocytosis activity of neutrophils was evaluated using the light microscope. The phagocytic index, the phagocytic number and the index of phagocytosis completion were determined. The suspension culture of Saccharomyces cerevisiae was used as a microbial agent. Preparations were stained using the Romanovsky-Himze methods. Neutrophilic leukocytes were separated from leukocyte suspension of peripheral blood. The expression of differentiation clusters CD3+, CD4+ and CD8+ on subpopulation of T- and B-cells were evaluated by indirect ELISA using monoclonal antibodies labeled with FITC-dye. To diagnose thymoma, we used spiral CT “Marconi” SeleCT/SP. For histologic study thymoma samples were fixed in 10% neutral formalin for 24 hours. The material was embedded in paraffin after posting through the chloroform in the usual way; then, sections with the thickness of 5-7 μm were prepared. Preparations were stained with hematoxylin and eosin.Results and conclusions. The severity of the clinical course of myasthenic syndrome on the background of thymoma does not depend on tumor size; it depends on the histologic type and immunological imbalance. The severest clinical picture was observed in patients with type AB thymoma and the least severe course of myasthenic symptoms was found in patients with lymphoid thymoma (type B1). Lymphoepithelial and epithelial thymomas were accompanied by similar immunological disorders. The reduction in the levels of CD3+ and CD4+ lymphocyte subpopulations can be used as a reliable diagnostic criterion. Lymphoid thymomas are characterized by a significant reduction in the indicators of the phagocytic index, the phagocytic number, and the level of CD8+ lymphocyte subpopulations as well as an increase in the level of CD4+ subpopulations. Surgical treatment as a method of choice in case of radical immunosuppression is indicated for all patients with confirmed thymoma regardless of its size and histological characteristics.

Immunostimulatory effects of the standardized extract of Tinospora crispa on innate immune responses in Wistar Kyoto rats.

Tinospora crispa (TC) has been used in folkloric medicine for the treatment of various diseases and has been reported for several pharmacological activities. However, the effects of TC extract on the immune system are largely unknown. Therefore, the present study was aimed to investigate the immunomodulatory effects of a standardized 80% ethanol extract of the stem of TC on innate immune responses. Male Wistar Kyoto rats were treated daily at 100 mg/kg, 200 mg/kg, and 400 mg/kg doses of the extract for 21 days by oral gavage. The immunomodulatory potential of TC was evaluated by determining its effect on chemotaxis and phagocytic activity of neutrophils isolated from the blood of rats. To further elucidate the mechanism of action, its effects on the proliferation of T- and B-lymphocytes and T-lymphocytes subsets (CD4+ and CD8+) and on the secretion of Th1 and Th2 cytokines were also monitored. The main components of the extracts, syringin and magnoflorine, were identified and quantitatively analyzed in the extracts by using a validated reversed-phase high-performance liquid chromatography method. It was observed that the chemotactic activity of neutrophils obtained from extract-treated rats increased as compared to controls. A dose-dependent increase in the number of migrated cells and phagocytosis activity of neutrophils was observed. Dose-dependent increase was also observed in the T- and B-lymphocytes proliferation stimulated with concanavalin A (5 μg/mL) and lipopolysaccharide (10 μg/mL), and was statistically significant at 400 mg/kg (P>0.01). Apart from cell-mediated immune response, the concentrations of Th1 (TNF-α, IL-2, and IFN-γ) and Th2 (IL-4) cytokines were significantly increased in sera of rats treated with different doses as compared with the control group. From these findings, it can be concluded that TC possesses immunostimulatory activity and has therapeutic potential for the prevention of immune diseases.

INFLUENCE OF IMMUNOLOGICAL DISORDERS ON AN OUTCOME FOR THE FIRST TIME DIAGNOSED INFILTRATIVE TUBERCULOSIS IN SOCIALLY SAFE PATIENTS

The assessment of immunological indicators in 76 first time diagnosed infiltrative tuberculosis of lungs in socially safe patients prior to treatment and in 2 months of chemotherapy was carried out. It is revealed that immunological disorders are connected with efficiency of tuberculosis treatment . It can be criterion of quality of provided chemotherapy. The activation of cell immunity, stimulation of phagocytosis activity of neutrophils would contribute for effective treatment of tuberculosis.

Phytochemical Qualitative Analysis and Immunomodulator Activity of Agaricus bisporous Ethanol Extract by Carbon Clearance Technique

The Agaricus bisporous have been collected and its extracts are subjected to qualitative analysis and immunomodulator activity. Quantitative analysis has been carried out to find some phytochemicals presents in the sample. These Ethanol extracts revealed with the presence of the constituents such as Sterols, Saponins, Coumarin Glycosides, Amino acid/ protein, Resins. A preliminary phytochemical screening has been conducted with the selected fungal extracts by using standard C.K.Kokate procedures and it is revealed that several secondary metabolites are present in the sample. In vivo the effect of ethanol extract of the two Agaricus bisporous has been applied on neutrophil phagocytosis activity. Various concentrations namely 50, 100 and 200 μg/ml of the ethanol extract has been tested for phagocytosis as neutrophil locomotion. This has been tested with Swiss albino mice with selected dose of ethanol concentrations in 50, 100 and 200 mg/kg body weight has been administrated in order to confirm this with macrophage phagocytosis activity by carbon clearance test. In vivo studies of Agaricus bisporous revels the moderate immune stimulator activity, thus this fungal species have demonstrated the prominent immunostimulator activity. The data generated in this study has the basics for its use as the therapeutic both as traditional and folk medicine as a widely as to be practiced.

Immunological Efficiency of MNRI Program at Treatment of Respiratory Diseases

Immunological Efficiency of MNRI Program at Treatment of Respiratory Diseases

Lidocaine Enhances Apoptosis and Suppresses Mitochondrial Functions of Human Neutrophil In Vitro

Neutrophils play an essential role in innate immunity and against infection. Mitochondria are organelles that produce energy in the form of adenosine triphosphate (ATP), which is required for maintaining the function and structure of cells and organs. Although local anesthetics (LAs) are widely used in clinical practice, it remains unclear whether LAs such as lidocaine, ropivacaine, and bupivacaine affect neutrophil mitochondrial functions. The aim of this study was to examine whether LAs have any effects on human neutrophil mitochondrial functions. Freshly isolated human neutrophils were incubated with lidocaine, ropivacaine, or bupivacaine. The oxidative burst and phagocytic activity of neutrophils and apoptotic rate of neutrophils were measured to assess the effect of LAs on neutrophil functions. The ATP concentration, mitochondrial transmembrane potential (Deltapsim), and neutrophil mitochondrial morphology were also measured to evaluate the effect of LAs on mitochondrial functions. Lidocaine (400 microm) induced a reduction in the oxidative burst and phagocytosis activity of neutrophils by approximately 20%. The ATP concentration was significantly lower in lidocain-treated neutrophils compared with control neutrophils (876 +/- 25 vs. 1,332 +/- 76 pmol/10(6) neutrophils; p < 0.05). Mitochondrial transmembrane potential (Deltapsim) was also significantly lower in lidocaine-treated neutrophils compared with control neutrophils (p < 0.05). Lidocaine also induced mitochondrial structural changes and induced apoptosis of neutrophils. Ropivacaine and bupivacaine had no effect on neutrophil functions including mitochondrial function. Furthermore, the study of mitochondrial function depletion demonstrated that neutrophil function requires active mitochondrial function. Although ropivacaine and bupivacaine had no effect on neutrophil and mitochondrial functions, lidocaine suppressed neutrophil function, inhibited ATP synthesis, reduced mitochondrial membrane potential, and induced apoptosis.

Inhibition of neutrophil infiltration by a malleable protein matrix of lactic acid bacteria-fermented whey proteins in vivo

A novel nutraceutical ingredient, the Malleable Protein Matrix (MPM), has previously demonstrated a significant anti-inflammatory effect in a systemic inflammatory disease model, comparable to conventional drugs. The objective of this study was to investigate the potential anti-inflammatory effects of MPM on neutrophil infiltration in vivo, phagocytosis activity as well as cytokine and chemokine production. Groups of ten C57BL\6J mice received water or MPM per os for a period of 2 weeks prior to the creation of a murine air pouch. The subsequent neutrophil recruitment and activities were characterized following lipopolysaccharide injection. In the water control group, the number of recruited cells was 1.8X10(7) cells/pouch, which was reduced to 9X10(6) cells/pouch with oral MPM consumption, representing an inhibition of 50% of infiltrating leukocytes. A considerable reduction in the cytokine and chemokine production, mostly TNFalpha, IL-1beta and IL-6 production in the MPM-treated group, suggested an inhibition of the mediators responsible for leukocyte extravasation. On the other hand, MPM consumption had no effect on neutrophil phagocytosis activity. MPM administration demonstrates a significant reduction of neutrophil infiltration associated with an inhibition of cytokine and chemokine production. The air pouch model shares similarities with in vivo characteristics of rheumatoid arthritis and neutrophilic diseases, both of which would benefit from this 50% inhibition of neutrophil infiltration induced by MPM.

Roxythromycin Stimulates the Mucociliary Activity of the Eustachian Tube and Modulates Neutrophil Activity in the Healthy Guinea Pig

Low-dosage, long-term erythromycin chemotherapy is useful in the treatment of chronic airway inflammatory diseases such as chronic sinusitis. The exact working mechanism of macrolides behind the clinical effectiveness still remains unclear. However, some have been considered including anti-inflammatory effect, effects on airway secretory functions, and steroid sparing effects. Otitis media with effusion (OME) is a chronic inflammatory disease in the tubotympanum. The epithelium of the tubotympanum is a modified respiratory epithelium. Therefore, macrolides might be effective in the treatment of patients with chronic OME. It was recently demonstrated that macrolides such as roxythromycin (RXM), enhance the ciliary activity in vitro. However, such ciliostimulatory effects found in an in vitro system are not always applicable to the mucociliary system in situ. The mucociliary system in situ might behave differently when in contact with RXM, and might deteriorate following oral administration of RXM. The present study aimed at investigating the in vivo effect of RXM on the mucociliary system in the tubotympanum and neutrophil activities of the guinea pig. The healthy guinea pigs were treated with oral administration of 5 or 50 mg/kg/day of RXM for 14 successive days, and the ciliary activity and the mucociliary clearance time of the tubotympanum was determined 24 h after the final administration. The ciliary activity in the Eustachian tube was significantly increased by oral administration of either dosage of RXM for 14 successive days. In addition, the mucociliary clearance velocity was accelerated by oral administration of such dosages of RXM. Therefore, the present data clearly demonstrate that RXM is a pharmacological agent with ciliostimulation and concurrent acceleration ability of the mucociliary clearance in the Eustachian tube. Oral administration of 5 as well as 50 mg/kg/day of RXM for 14 successive days did not significantly affect the phagocytosis activity of neutrophils but significantly increased the superoxide production of neutrophils. Since the effusions are considered to be the result of inflammatory events in the tubotympanum, the increased superoxide production activity of neutrophils confirmed in our study should result in an increased killing activity of infectious pathogens, thereby leading to control of the disease chronicity. In conclusion, our study argues in favor of the clinical usefulness of RXM in the treatment of OME.

Impaired neutrophil function in chronic renal failure--dysregulation of surface adhesion molecule expression and phagocytosis

To elucidate the impaired neutrophil function in patients with chronic renal failure, we analyzed the expression of the adhesion molecules, LAM-1, LFA-1, Mac-1, gp150/95 and phagocytosis activity of neutrophils in predialysis and hemodialysis patients by flow cytometry. Further, the response to granulocyte colony-stimulating factor (G-CSF), N-formyl-methionyl-leucyl-phenylalanine (fMLP) and tumor necrosis factor alpha (TNF alpha) were investigated. In hemodialysis patients, the expression of LAM-1 was decreased and that of MAC-1 was increased, indicating the activation of neutrophils. Also in predialysis patients, the same condition of "low LAM-1, high MAC-1" was observed, but to a lesser degree. Phagocytosis activity was significantly decreased in hemodialysis patients, whereas the neutrophils of predialysis patients showed almost the same phagocytosis activity compared to the controls. The responses to G-CSF, fMLP, TNF alpha were significantly reduced both in hemodialysis and predialysis patients. The inadequate activation of neutrophils and impaired response to stimulation may play an important role in uremic patients with regard to increased susceptibility to infections.