pH Dissociation Research Articles

Isolation of specific antibody under conditions of low ionic strength

Specific rabbit and goat antibodies to tobacco mosaic virus, bovine serum albumin and human gamma globulin have been isolated by acid dissociation from the antigen in the absence of salt. Maximum recovery of 96–100% of active antibody occurred at pH 2.8–2.9. The amount of antibody recovered within any given pH range was highly reproducible. The average antibody affinity was found to be correlated with the pH of dissociation and to decrease exponentially with the length of exposure to acid conditions.

Structure and properties of hemocyanins: VIII. Microheterogeneity of α-hemocyanin of Helix pomatia

The degree of dissociation of Helix pomatia α-hemocyanin is independent of its concentration. The dissociation products can be separated by ultracentrifugation or gel filtration. The isolated fractions do not reequilibrate. Subfractions of α-hemocyanin differ from total α-hemocyanin is a pH-dependent dissociation behaviour. It is suggested that α-hemocyanin is a population of closely similar molecular species differing in pH of dissociation. Independent evidence for microheterogeneity is obtained from reversible boundary spreading of α-hemocyanin and some of its dissociation products by electrophoresis on cellulose acetate.