Abstract
The degree of dissociation of Helix pomatia α-hemocyanin is independent of its concentration. The dissociation products can be separated by ultracentrifugation or gel filtration. The isolated fractions do not reequilibrate. Subfractions of α-hemocyanin differ from total α-hemocyanin is a pH-dependent dissociation behaviour. It is suggested that α-hemocyanin is a population of closely similar molecular species differing in pH of dissociation. Independent evidence for microheterogeneity is obtained from reversible boundary spreading of α-hemocyanin and some of its dissociation products by electrophoresis on cellulose acetate.
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