The results of the first lichenological studies in the old park of the museum-reserve “Gorki Leninsky” (Leninsky urban district of the Moscow oblast) are presented. The checklist of lichen biota is given including 71 species of lichens and 3 species of allied non-lichenized fungi from 40 genera, 25 families. Acarospora moenium (Vain.) Räsänen, Lecania erysibe (Ach.) Mudd are new species for Moscow oblast. Four indicator species of biologically valuable forest and park communities are identified: Alyxoria varia, Biatoridium monasteriense, Chaenotheca stemonea, Physconia perisidiosa. The absolute index of lichen biota species richness of the park of the museum-reserve “Gorki Leninsky” is higher than in the previously studied parks of the museum-reserve “Abramtsevo”, the museum-reserve of A. S. Pushkin and the museum-estate “Ostafyevo” – “Russian Parnassus”. However, the relative index of species richness (Menchinic Index) of the lichen biota of the park “Gorki Leninsky” is lower than that of the park “Abramtsevo”, located in the area with less anthropogenic load. Nitrophilic species prevail over acidophilic species in the lichen cover of oak and birch, which have an “acidic” pH of bark in the background conditions, in the surveyed part of the territory of the park “Gorki Leninsky”. The revealed ratio of acidophytes and nitrophytes in the lichen cover of oak and birch, and the absence of species highly sensitive to dusting and air pollution indicate a significant anthropogenic load on the surveyed park community, in particular, nitrogen pollution.