In this study, the experimental thermodynamic performance and environmental impacts of a vehicle air conditioner using HFO-1234yf with a suction line heat exchanger are studied and compared with HFC-134a results. The influences of air flow rate and air temperature at the evaporator inlet, condenser side air face velocity, and the compressor speeds on the thermodynamic performance of the system are investigated. The results showed that HFO-1234yf has a 12% improved coefficient of performance with 8% higher exergy efficiency than HFC-134a. However, HFO-1234yf has marginally poor performance at higher compressor speeds due to more irreversibilities occurred in the compressor and evaporator. The average SLHX effectiveness was observed to be 45% for HFC-134a, while it was 41% for HFO-1234yf. The HFO-1234yf has a 33.9% and 29.4% and 45.1% lower total equivalent warming impact than HFC-134a for petrol, diesel, and liquefied petroleum gas vehicles, respectively. This study paves a way to promote the use of HFO-1234yf instead of HFC-134a without major modifications in the vehicle air conditioner unit.