Pesticide Exposure Research Articles

Effects of the insecticide acetamiprid and the fungicide azoxystrobin on locomotion activity and mushroom bodies of solitary bee Centris analis

Pesticide use is a major factor contributing to the global decline in bee populations. Sublethal effects, such as behavior alterations, are neglected in pesticide regulation for pollinators. However, these effects can bring important information to understanding the impacts of pesticides on bees' daily activities. In this study, we aimed to investigate the effects of the insecticide acetamiprid (7 ng/μL) and the fungicide azoxystrobin (10 ng/μL) on the behavior of the Neotropical solitary bee Centris analis. Female and male bees were exposed to these chemicals continuously for 48 h, followed by an additional 48 h without contaminated food, totaling 96 h of observation. We used five experimental groups: control, solvent control, insecticide, fungicide, and pesticide mixture (insecticide + fungicide). Behavioral alterations based on locomotion and light response were assessed by video tracking at 48 (end of pesticide exposure) and 96 h (end of bioassay). In addition, after recording bees at 96 h, the individuals were anesthetized for brain collection and histological evaluation of mushroom bodies to evaluate if pesticides can damage their neurons and impair the cognitive processes and responses of bees to sensory stimuli. Bees exposed to acetamiprid and pesticide mixture showed lethargic movements and impaired locomotion at 48 h. Notably, these behavioral effects were no longer evident after the bees consumed uncontaminated food for an additional 48 h, totaling 96 h from the start of pesticide exposure. Only fungicide exposure did not result in any behavioral or brain histological changes. Therefore, our study showed that acetamiprid at an estimated residual concentration, despite being classified as having low toxicity for bees, can cause significant initial locomotion disruption in solitary bees. These findings highlight the importance of considering sublethal effects in environmental risk assessment.

The evaluation of pesticide exposure in cannabis using advanced analytical techniques: A review

The evaluation of pesticide exposure in cannabis using advanced analytical techniques: A review

Prenatal Pesticide Exposure and Neurodevelopment

Prenatal Pesticide Exposure and Neurodevelopment

Availability of Using Honeybees and Hive Products as Bioindicators of Ambient Pesticide Exposure in Taiwan.

Honeybees and hive products could be used as bioindicators of pesticide exposure in surrounding areas, but the associations have rarely been examined. We collected samples of bees, hive products and environmental dust from 12 apiaries during the blooming season in eastern Taiwan and assessed the relationships between pesticides in apiarian samples and the environment. Samples were analyzed for 14 pesticides using gas or liquid chromatography coupled with mass spectrometry. Sick bees, dead bees, bee pollen, beeswax and environmental dust in the outer rings (>150 m) surrounding the apiaries were contaminated with high levels of pesticides (mean concentration: >270 ng/g in total). In terms of concentrations of all pesticides, insecticides, herbicides and fungicides, most apiarian sample matrices were significantly correlated with environmental dust within a range of 2.5 km (ρ > 0.6, p < 0.05), suggesting their potential as bioindicators. Of those apiarian matrices with high contamination contents, dead bees or beeswax may be a good bioindicator for all pesticides but not for herbicides, because of the insignificant correlation with environmental dust (ρ < 0.5). For all types of pesticides, we recommend sick bees and bee pollen as choices for bioindicators, because of their high contamination levels for detection and complete representativeness of the environment.

Immunological and physiological responses to predation risk and sublethal concentrations of chlorothalonil and β-endosulfan in Lithobates taylori (Anura: Ranidae) tadpoles

Pesticide exposure and its interaction with other natural stressors can play a role in amphibian population declines because disruptions in stress hormone regulatory mechanisms may inhibit immune responses during metamorphosis. Here, we determined the interactive effects of predation risk and sublethal concentration of two pesticides on immunological and physiological responses in tadpoles of the tropical frog Lithobates taylori. Using mesocosms, we used chronic exposure to three levels of chlorothalonil and β-endosulfan in the presence or absence of Odonate larvae. Our results show that β-endosulfan in high concentrations reduced the weight of the tadpoles and increased the neutrophil count and corticosterone (CORT) levels. Larval development was accelerated by high concentrations of chlorothalonil. Also, this pesticide in low and high concentrations increases the absolute values of lymphocytes. Tadpoles exposed to chlorothalonil increased the numbers of monocytes (in low concentrations), and lymphocytes (in high and low concentrations). The interactions of the low concentrations of both pesticides with and without the predator's presence also increased the number of lymphocytes. A combination of pesticides increases the number of lymphocytes in the blood due to synergistic cytotoxicity. This study proves that β- endosulfan elevates circulating CORT and thus generates physiological stress in tadpoles. Given that both pesticides are widely used within the distribution of L. taylori in Costa Rica, it is likely that tadpoles’ development and immune function are altered by pesticide use. In combination with stressors such as emerging diseases and altered precipitation regimes, widespread agrochemical uses likely caused this species enigmatic decline in recent decades.

Mixture toxic mechanism of phoxim and prochloraz in the hook snout carp Opsariichthysbidens

Pesticides are usually found as mixtures in surface water bodies, even though their regulation in aquatic ecosystems is usually approached individually. In this context, this work aimed to investigate the enzymatic- and transcriptional-level responses after the mixture exposure of phoxim (PHX) and prochloraz (PRC) in the livers of hook snout carp Opsariichthys bidens. These data exhibited that co-exposure to PHX and PRC induced an acute synergistic impact on O. bidens. The activities of catalase (CAT), superoxide dismutase (SOD), carboxylesterase (CarE), and caspase3 varied significantly in most of the individual and combined challenges relative to basal values, indicating the activation of oxidative stress, detoxification dysfunction, as well as cell apoptosis. Besides, the transcriptional levels of five genes (gst, erα, mn-sod, cxcl-c1c, and il-8) exhibited more pronounced changes when subjected to combined pesticide exposure in contrast to the corresponding individual compounds. The findings revealed the manifestation of endocrine dysfunction and immune disruption. These results underscored the potential biochemical and molecular toxicity posed by the combination of PHX and PRC to O. bidens, thereby contributing to a deeper comprehension of the ecological toxicity of pesticide mixtures on aquatic organisms. Importantly, the concurrent presence of PHX and PRC might exacerbate hepatocellular damage in hook snout carps, potentially attributable to their synergistic toxic interactions. This study underscored the toxicological potency inherent in the co-occurrence of PHX and PRC in influencing fish development, thereby offering valuable insights for the risk assessment of pesticide mixtures and the safeguarding of aquatic organisms.

The Association between Maternal Endocrine-Disrupting Chemical Exposure during Pregnancy and the Incidence of Male Urogenital Defects: A Systematic Review and Meta-Analysis.

The increasing incidence of hypospadias and cryptorchidism, coupled with the widespread presence of endocrine-disrupting chemicals (EDCs), has raised concerns about the potential impact of these environmental factors on male urogenital development. This systematic review and meta-analysis aims to evaluate the association between maternal exposure to various EDCs and the risk of hypospadias and cryptorchidism. We conducted a comprehensive search of PubMed, Scopus, Web of Science, and Cochrane databases from inception until May 2024. We included case-control and cohort studies that examined the association between maternal EDC exposure and hypospadias or cryptorchidism, reporting adjusted odds ratios (aOR) or crude odds ratios (cOR). Data were extracted and pooled using a random effects model, and heterogeneity was assessed using the Q test and I-square statistics. The risk of bias was evaluated using the Newcastle-Ottawa Scale (NOS). A total of 48 studies were included in the systematic review, with 46 studies included in the meta-analysis. The pooled analysis revealed a significant association between maternal EDC exposure and an increased risk of hypospadias (aOR = 1.26, 95% CI: 1.18-1.35, p < 0.0001) and cryptorchidism (aOR = 1.37, 95% CI: 1.19-1.57, p < 0.001). Subgroup analyses showed that exposure to pesticides, phthalates, alkyl phenolic compounds (ALKs), and heavy metals significantly increased the risk of hypospadias. In contrast, polychlorinated biphenyls (PCBs) did not show a significant association. Significant associations were found with pesticide and PCB exposure for cryptorchidism, but not with phthalate, ALK, or heavy metal exposure. Maternal exposure to certain EDCs is associated with an increased risk of hypospadias and cryptorchidism in male children. These findings underscore the importance of addressing environmental and occupational exposures during pregnancy to mitigate potential risks. Further research is needed to elucidate the mechanisms by which EDCs affect urogenital development and to develop effective interventions to reduce exposure among vulnerable populations.

Impact of Pesticide Training on Safety Climate Perception Among French Cereal Farmers.

Background: The training of farmers in pesticide safety is of prime importance for reducing pesticide use and exposure through the implementation of sustainable management practices. This study aimed to assess the impact of compulsory training on the knowledge and perceptions of cereal farmers, with the help of a safety climate scale. Method: We approached cereal farmers throughout France during compulsory training and certification procedures for pesticide-related activities. Trainees were asked to complete a safety climate questionnaire at the start and end of the course. In total, 733 cereal farm managers or workers completed the questionnaire at the start of the study, 131 of whom declined to complete the questionnaire at the end of the training session, leaving 602 subjects available for pre-training/post-training comparisons. Statistical analyses were based on paired t-tests and mixed models for repeated measures. Findings: The mean safety climate score increased from 82.37 to 88.22 after the training course (7.1 %, p < .001) in both univariate and multivariate analyses. Mean increases were also found for each of its seven dimensions (p < .001), ranging from 2.8% for "rules and best practices" to 12.4% for "communication and feedback." Few covariate-by-time interactions were found to be significant. Conclusion/Application to Practice: This study demonstrates that pesticide training is highly effective in increasing safety climate perception among cereal farmers and provides hints for improving the design of educational programs. Promoting the development and facilitation of lifelong learning with continuously updated training programs should be a top priority for minimizing pesticide exposure.

Honeybee gut bacterial strain improved survival and gut microbiota homeostasis in Apis mellifera exposed in vivo to clothianidin.

The present work suggests that administration of bacterial strains isolated from honeybee gut may promote recovery of gut microbiota homeostasis after prolonged clothianidin exposure, while improving survival. This study highlights that gut bacterial strains hold promise for developing efficient microbial formulations to mitigate environmental pesticide exposure in honeybee colonies.

Pollinator visitation decline due to pesticide application beyond threshold frequency brings down crop yield

Abstract The deleterious effects of pesticides on pollinating insect abundance are well established but the relationship among pesticide application, pollinator decline and the corresponding change in marketable yield is not fully understood. In this study, we assessed what level of pesticide exposure causes maximum pollinator loss and at that level of pollinator loss if there is any crop yield loss. We primarily assessed pollinator crop flower visitation in 27 aubergine farms with different degrees of pesticide application. Subsequently, we also observed pollinator activity and aubergine yield (fruit set) in a semi‐controlled field experiment with four different pesticide treatments. Pesticide application frequency was the most important driver of pollinator visitation compared with quantity. Pesticide spray above once a week caused a drastic drop in pollinator visitation in a non‐linear fashion showing a threshold of pesticide application. In high pesticide frequency experimental plots, pollinator visitation was significantly lower than in control, low and medium frequency plots. This resulted in lowering of aubergine fruit set by 27.4% in high pesticide frequency plots. Use of synthetic pesticides to safeguard yield seems to be doing just the opposite by bringing down yield from pesticide‐induced pollinator limitation.

Pesticide residues and polyphenols in urine – A combined LC-HRMS screening to reveal intake patterns

Human exposure to pesticides in the general population occurs mainly through food consumption. However, specific dietary habits or food products that contribute to pesticide exposure are often unknown. In this study, we propose a combined screening for polyphenols and pesticide residues by liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS) to assess the diet and the associated pesticide exposure. We measured 587 urine samples from women around the 34th week of pregnancy of a prospective mother–child cohort. A non-targeted screening for flavonoid-like compounds related to fruit and vegetable consumption was performed, prioritizing 164 features and identifying a total of 46 features by spectral library search. Based on a subset of markers, k-means clustering was performed, leading to four clusters with presumably similar dietary habits. The clusters were compared against food questionnaire data collected within the period of sample collection. Suspect screening of more than 500 pesticide residues including metabolites was performed, with a total of 40 residues being reported for 27 different pesticides. The detection of pesticide residues was compared across the different clusters of dietary habits. Indications were found that pyrimethanil metabolites might be associated with the consumption of citrus fruits or derivate products. We demonstrate that the method used has the potential to reveal patterns of pesticide intake from specific food commodities.

Respiratory system: Highly exposed yet under-reported organ in pyrethrin and pyrethroid toxicity.

Pyrethrin and pyrethroid are a relatively new class of pesticides with potent insecticidal properties. Pyrethrins are naturally occurring pesticides obtained from the Chrysanthemum cinerariaefolium flower, while pyrethroids are their synthetic derivatives. They are widely used as the insecticides of choice in agriculture, veterinary medicine, public health programs, and household activities. Pyrethrin, being a broad-spectrum insecticide kills a wide range of pests, while pyrethroids last longer in the environment owing to low susceptibility to sunlight, and greater stability and efficacy than parent molecules. Humans can be exposed through inhalation, ingestion, and dermal routes. Indoor usage of an insecticide poses a serious risk to human health, especially to women, children, and stay-at-home people. Although pyrethrin and pyrethroid are generally considered safe, sustained skin or inhalation exposure or direct contact with open wounds results in higher toxicity to mammals. There is a paucity of data on the impact of pyrethrin and pyrethroid on overall pulmonary health. The respiratory system, from the nose, nasal passages, airways, and bronchi to the pulmonary alveoli, is vulnerable to environmental contaminants such as pesticides because of its anatomical location as well as being a highly blood profused organ. Under and over-functioning of the respiratory system triggers diverse pathologies such as serious infections, allergies, asthma, metastatic malignancies, and auto-immune conditions. While the association between workplace-related pesticide exposures and respiratory diseases and symptoms is well documented, it is important to understand the adverse health impact of pyrethrin and pyrethroid on the general population for awareness and also for better regulation and implementation of the law.

Changes in bumblebee queen gut microbiotas during and after overwintering diapause.

Bumblebees are key pollinators with gut microbiotas that support host health. After bumblebee queens undergo winter diapause, which occurs before spring colony establishment, their gut microbiotas are disturbed, but little is known about community dynamics during diapause itself. Queen gut microbiotas also help seed worker microbiotas, so it is important that they recover post-diapause to a typical community structure, a process that may be impeded by pesticide exposure. We examined how bumblebee queen gut microbiota community structure and metabolic potential shift during and after winter diapause, and whether post-diapause recovery is affected by pesticide exposure. To do so, we placed commercial Bombus impatiens queens into diapause, euthanizing them at 0, 2 and 4 months of diapause. Additionally, we allowed some queens to recover from diapause for 1 week before euthanasia, exposing half to the common herbicide glyphosate. Using whole-community, shotgun metagenomic sequencing, we found that core bee gut phylotypes dominated queen gut microbiotas before, during and after diapause, but that two phylotypes, Schmidhempelia and Snodgrassella, ceased to be detected during late diapause and recovery. Despite fluctuations in taxonomic community structure, metabolic potential remained constant through diapause and recovery. Also, glyphosate exposure did not affect post-diapause microbiota recovery. However, metagenomic assembly quality and our ability to detect microbial taxa and metabolic pathways declined alongside microbial abundance, which was substantially reduced during diapause. Our study offers new insights into how bumblebee queen gut microbiotas change taxonomically and functionally during a key life stage and provides guidance for future microbiota studies in diapausing bumblebees.

Impact of Organochlorine Pesticides Exposure on Histone Modification H3K9ac: Implications for Unexplained Recurrent Miscarriage.

Epigenetic alterations, changes in gene expression without DNA sequence modifications, are associated with various health disorders, including reproductive health issues. These alterations can be influenced by environmental factors such as pesticides. This study aimed to explore the relationship between exposure to Organochlorine Pesticides (OClPs) and the histone modification mark H3K9ac in the placenta and fetal tissue, in the context of unexplained recurrent miscarriage (URM). In the case-control study, serum samples from 73 women with URM and 30 healthy women were examined for the presence of OClPs, which include 2,4-DDT, 2,4-DDE, 4,4-DDT, 4,4-DDE, α-HCH, β-HCH, and γ-HCH, using gas chromatography. Western blot analysis was used to assess H3K9ac expression in placental and fetal tissues. In the URM group, significant increases were observed in the values of α-HCH, β-HCH, 2,4-DDE, and 4,4-DDE, as well as in the concentration of total OClPs (Ʃ3HCH, Ʃ2DDE, Ʃ2DDT, and Ʃ7OClP), compared to controls. While H3K9ac levels in fetal tissue showed no significant difference, a notable decrease was found in the placental tissue of the URM. In the placenta tissue of URM, logistic regression analysis also revealed a significant inverse correlation between the toxins α-HCH, 2,4-DDE, 4,4-DDE, 4,4-DDT, total OClPs, and reduced H3K9ac expression. Our findings suggest that OClPs exposure may contribute to URM by reducing H3K9ac expression in the placenta, potentially affecting placental growth and immune tolerance. This underscores the need for further investigation into the involved mechanisms and potential therapeutic interventions, and the importance of OClPs regulation for reproductive health protection.

Developmental approach of safety in ergonomics/human factors: insights of constructed safety in six work environments

This article brings together works on the concept of constructed safety in ergonomics, carried out over the last twenty-five years. Firstly, we situate this approach to safety in relation to previously developed existing models (e.g. regulated and managed safety) with regard to the development of activity-centred ergonomics. We then present six research actions in activity-centred ergonomics from a selection of different fields, from small companies to the industry of the future in an international group: public works, hospitals, aeronautical industry, railway transport, agriculture, and chemical industry, in order to describe constructed safety applications. The results highlight that constructed safety is respectively raised by mutual knowledge between workers and management, collective decision making, collective reflexive work on safety rules, spatiotemporal articulation of the different safety sources, knowledge integration on pesticide exposure situations by designers and regulation, social regulation sustaining risk understanding and safety aspect involving a diversity of actors (workers, preventionists, managers, local residents and public authorities). By focusing on the analysis of actual safety practices in real work and real exposure situations, constructed safety aims to account for the way in which safety is deployed on a daily basis to meet production and health objectives. This understanding contributes to the design of safe work systems in a developmental way and to propose an operating model of constructed safety.

Pesticide Exposure and Neurobehavioral Performance across various Agricultural Spray Seasons in Ecuador, and implementation of Agroecological Production Alternatives

Pesticide Exposure and Neurobehavioral Performance across various Agricultural Spray Seasons in Ecuador, and implementation of Agroecological Production Alternatives

Pesticide Exposure and Children’s Neurodevelopment at Five and Six Years in the ISA Birth Cohort Costa Rica

Pesticide Exposure and Children’s Neurodevelopment at Five and Six Years in the ISA Birth Cohort Costa Rica

Pesticide Exposure and Pregnancy Outcomes Among Women Living in the Brazilian Amazon – Preliminary Findings

Pesticide Exposure and Pregnancy Outcomes Among Women Living in the Brazilian Amazon – Preliminary Findings

Socio-sanitary vulnerability and pesticides exposure in horticultural work, Cordoba, Argentina.

Socio-sanitary vulnerability and pesticides exposure in horticultural work, Cordoba, Argentina.

Pesticide exposure during pregnancy and risk of early onset asthma and wheeze in the offspring

Pesticide exposure during pregnancy and risk of early onset asthma and wheeze in the offspring