Artemisia annua L. [family Asteraceae (Compositae)] is a well-known medicinal herb with a broad spectrum of applications in pharmaceuticals and cosmetics. The present study aimed to investigate the variation of essential oil (EO) composition from the plant aerial parts, harvested in rainy (AAREO) and winter (AAWEO) seasons. GC-MS analysis of AAREO and AAWEO led to the identification of 75 and 59 constituents, respectively. AAREO was dominated by camphor (14.1%), germacrene-D (9.0%), β-caryophyllene (8.7%), and eucalyptol, (4.2%) whereas AAWEO was abundant in camphor (17.9%), eucalyptol (14.6%) and artemisia alcohol (3.5%). Both EOs showed antifeedant activity against Spodoptera litura and nematocidal activity versus Meloidogyne incognita. The in silico test proved that the major EOs compounds showed a good score of docking with acetylcholinesterase (PDB ID: 2ACE) compared to carbofuran.