From the view point of the environmental protection and convenience for transportation, a bicycle is one of the effective forms of the personal mobility vehicles. It is known that a small wheel bicycle running at low speed has less upright stability. In this study, steer-by-wire system is used to steer and stabilize a small wheel bicycle. In a bicycle with Steer-by-wire system, the mechanical connection between handlebar and steer-assembly is replaced by electronic connection with actuators, sensors and a controller. The system can enhance the upright stability of a bicycle by a control system. This study aims to enhance the upright stability of a small wheel bicycle at low speed. Firstly, we designed the mechanism of steer-by-wire system. The system has tracking torque and stabilizing torque. The former reduces the difference between handle and steer angle to a minimum. The later makes a bicycle upright when it leans. Secondly, we made the experimental model of the small wheel bicycle with steer-by-wire system. Finally, we conducted a running experiment to evaluate the tracking performance of steer-by-wire system. From the time history response of the handle and steer angle, the good performance was confirmed.