Persistent Current Research Articles

Density-functional study of persistent currents in quantum rings

The density functional theory is used to study persistent currents in two-dimensional quantum rings containing several electrons. We find a series of magic numbers for the total angular momentum of electrons in the strong magnetic field and show that changes in the angular momentum of electrons lead to persistent current oscillations. We suggest an empirical expression for the period of persistent current in quantum rings and examine the effect of Coulomb interaction on the properties of persistent currents.

Study of thermomagnetic properties of the diatomic particle using hyperbolic function position dependent mass under the external hyperbolic magnetic and AB force

In this present work, we study the approximate solution of the Schrodinger equation with hyperbolic function position-dependent mass for a symmetrical Modified Poschl-Teller potential. We consider the system influenced by the external hyperbolic magnetic and Aharonov-Bohm (AB) forces. By using the Laplace transform method, the second-order differential equation of the Schrodinger equation is reduced to a first-order differential equation and so the eigenfunction and energy eigenvalues are obtained. The behavior of the bound state energy levels was demonstrated and analyzed using the computational method for various values of the potential parameter, mass parameter, external magnetic force, and AB force. Moreover, the thermomagnetic properties of the system were analyzed for H2, LiH, and HCl diatomic particles, involving the vibrational mean energy, vibrational free energy, vibrational entropy, specific heat capacity, magnetization, magnetic susceptibility, and persistent current.

Variational quantum eigensolver for SU(N) fermions

Variational quantum algorithms aim at harnessing the power of noisy intermediate-scale quantum (NISQ) computers, by using a classical optimizer to train a parameterized quantum circuit to solve tractable quantum problems. The variational quantum eigensolver (VQE) is one of the aforementioned algorithms designed to determine the ground-state of many-body Hamiltonians. Here, we apply the VQE to study the ground-state properties of N-component fermions. With such knowledge, we study the persistent current of interacting SU(N) fermions, which is employed to reliably map out the different quantum phases of the system. Our approach lays out the basis for a current-based quantum simulator of many-body systems that can be implemented on NISQ computers.

Adaptive Neural Network-Based Tracking Control of Underactuated Offshore Ship-to-Ship Crane Systems Subject to Unknown Wave Motions Disturbances

As a typical underactuated mechanical system, offshore ship-mounted cranes are widely used to carry out the tasks of transferring cargos from one ship to another in the marine environment. Different from land-fixed cranes as well as traditional harbor cranes, offshore ship-to-ship crane systems work in two noninertial (ship) frames, while the target locations of the cargos are also inevitably influenced by the movements of the target ship. Besides, various external disturbances, which are caused by persistent sea waves, sea winds, or currents, etc., bring much more challenges to the control task of offshore ship-to-ship crane systems. To properly address these practical problems, in this article, we propose an increased adaptive neural network (NN)-based anti-swing tracking control strategy for such coordinated offshore crane systems, with a ship-motion prediction algorithm suggested to generate the target trajectories for cargos, and an adaptive NN proposed to deal with complicated unknown wave-induced disturbances. Bounded tracking performance is also guaranteed through a complete Lyapunov-based stability analysis. To the best of our knowledge, without any simplification of the original nonlinear dynamics, this article provides a high-performance adaptive control approach to deal with the anti-interference tracking control problem for offshore ship-to-ship crane systems, which are subjected to trajectories uncertainties as well as unknown wave motions disturbances. Furthermore, comparative hardware experimental results are presented to demonstrate the efficiency of the proposed control method.

Design of a 60 kA Flux Pump for Fusion Toroidal Field Coils

High-temperature superconducting (HTS) magnets offer a novel pathway to magnetically confined fusion energy generation. Reactor-scale fusion devices require tens of kA of current to generate large toroidal fields for magnetic confinement. Operating HTS magnets at kA-current levels is difficult due to unavoidable joint resistances that require active driving of current. The large footprint and energy demand of a solid-state power supply and multi-kA current leads present significant constraints on the size, cost and efficiency of a fusion tokamak. An alternative approach is to use an HTS flux pump, which can generate and sustain persistent currents in HTS coils at the kA-levels required by fusion magnet systems. Here, we outline the design of a 60 kA transformer-rectifier flux pump for energizing and sustaining an 80 mH HTS fusion magnet. The flux pump is driven by an iron-core transformer with copper primary and HTS secondary windings. This is rectified by switching elements in the superconducting circuit into a uni-directional dc voltage output which charges the inductive load. To charge the load coil to 60 kA in 24 hours, an output dc voltage of 55 mV is needed. However, only 6 mV is required to maintain a long-term steady current of 60 kA. Such a device can significantly reduce the cost and footprint of future fusion reactor designs.

Anti-invasive effects of minoxidil on human breast cancer cells: combination with ranolazine

A plethora of ion channels have been shown to be involved systemically in the pathophysiology of cancer and ion channel blockers can produce anti-metastatic effects. However, although ion channels are known to frequently function in concerted action, little is known about possible combined effects of ion channel modulators on metastatic cell behaviour. Here, we investigated functional consequences of pharmacologically modulating ATP-gated potassium (KATP) channel and voltage-gated sodium channel (VGSC) activities individually and in combination. Two triple-negative human breast cancer cell lines were used: MDA-MB-231 and MDA-MB-468, the latter mainly for comparison. Most experiments were carried out on hypoxic cells. Electrophysiological effects were studied by whole-cell patch clamp recording. Minoxidil (a KATP channel opener) and ranolazine (a blocker of the VGSC persistent current) had no effect on cell viability and proliferation, alone or in combination. In contrast, invasion was significantly reduced in a dose-dependent manner by clinical concentrations of minoxidil and ranolazine. Combining the two drugs produced significant additive effects at concentrations as low as 0.625 μM ranolazine and 2.5 μM minoxidil. Electrophysiologically, acute application of minoxidil shifted VGSC steady-state inactivation to more hyperpolarised potentials and slowed recovery from inactivation, consistent with inhibition of VGSC activation. We concluded (i) that clinically relevant doses of minoxidil and ranolazine individually could inhibit cellular invasiveness dose dependently and (ii) that their combination was additionally effective. Accordingly, ranolazine, minoxidil and their combination may be repurposed as novel anti-metastatic agents.

Effects of reciprocal inhibition and whole-body relaxation on persistent inward currents estimated by two different methods.

Persistent inward currents (PICs) are crucial for initiation, acceleration, and maintenance of motoneuron firing. As PICs are highly sensitive to synaptic inhibition and facilitated by serotonin and noradrenaline, we hypothesised that both reciprocal inhibition (RI) induced by antagonist nerve stimulation and whole‐body relaxation (WBR) would reduce PICs in humans. To test this, we estimated PICs using the well‐established paired motor unit (MU) technique. High‐density surface electromyograms were recorded from gastrocnemius medialis during voluntary, isometric 20‐s ramp, plantarflexor contractions and decomposed into MU discharges to calculate delta frequency (ΔF). Moreover, another technique (VibStim), which evokes involuntary contractions proposed to result from PIC activation, was used. Plantarflexion torque and soleus activity were recorded during 33‐s Achilles tendon vibration and simultaneous 20‐Hz bouts of neuromuscular electrical stimulation (NMES) of triceps surae. ΔF was decreased by RI (n = 15, 5 females) and WBR (n = 15, 7 females). In VibStim, torque during vibration at the end of NMES and sustained post‐vibration torque were reduced by WBR (n = 19, 10 females), while other variables remained unchanged. All VibStim variables remained unaltered in RI (n = 20, 10 females). Analysis of multiple human MUs in this study demonstrates the ability of local, focused inhibition to attenuate the effects of PICs on motoneuron output during voluntary motor control. Moreover, it shows the potential to reduce PICs through non‐pharmacological, neuromodulatory interventions such as WBR. The absence of a consistent effect in VibStim might be explained by a floor effect resulting from low‐magnitude involuntary torque combined with the negative effects of the interventions.Key points Spinal motoneurons transmit signals to skeletal muscles to regulate their contraction. Motoneuron firing partly depends on their intrinsic properties such as the strength of persistent (long‐lasting) inward currents (PICs) that make motoneurons more responsive to excitatory input.In this study, we demonstrate that both reciprocal inhibition onto motoneurons and whole‐body relaxation reduce the contribution of PICs to human motoneuron firing. This was observed through analysis of the firing of single motor units during voluntary contractions.However, an alternative technique that involves tendon vibration and neuromuscular electrical stimulation to evoke involuntary contractions showed less effect. Thus, it remains unclear whether this alternative technique can be used to estimate PICs under all physiological conditions.These results improve our understanding of the mechanisms of PIC depression in human motoneurons. Potentially, non‐pharmacological interventions such as electrical stimulation or relaxation could attenuate unwanted PIC‐induced muscle contractions in conditions characterised by motoneuron hyperexcitability.

On the Localization of Persistent Currents Due to Trapped Magnetic Flux at the Stacking Faults of Graphite at Room Temperature

Granular superconductivity at high temperatures in graphite can emerge at certain two-dimensional (2D) stacking faults (SFs) between regions with twisted (around the c-axis) or untwisted crystalline regions with Bernal (ABA…) and/or rhombohedral (ABCABCA…) stacking order. One way to observe experimentally such 2D superconductivity is to measure the frozen magnetic flux produced by a permanent current loop that remains after removing an external magnetic field applied normal to the SFs. Magnetic force microscopy was used to localize and characterize such a permanent current path found in one natural graphite sample out of ∼50 measured graphite samples of different origins. The position of the current path drifts with time and roughly follows a logarithmic time dependence similar to the one for flux creep in type II superconductors. We demonstrate that a ≃10 nm deep scratch on the sample surface at the position of the current path causes a change in its location. A further scratch was enough to irreversibly destroy the remanent state of the sample at room temperature. Our studies clarify some of the reasons for the difficulties of finding a trapped flux in a remanent state at room temperature in graphite samples with SFs.

Gold-gold luminosity increase in RHIC for a beam energy scan with colliding beam energies extending below the nominal injection energy

The Beam Energy Scan phase II (BES-II), performed in the Relativistic Heavy Ion Collider (RHIC) from 2019 to 2021, explored the phase transition between quark-gluon plasma and hadronic gas. BES-II exceeded the goal of a fourfold increase in the average luminosity over that achieved during Beam Energy Scan phase I (BES-I), at five gold beam energies: 9.8, 7.3, 5.75, 4.59, and 3.85 GeV/nucleon. This was accomplished by addressing several beam dynamics effects, including intrabeam scattering, beam-beam, space charge, beam instability, and field errors induced by superconducting magnet persistent currents. Some of these effects are especially detrimental at low energies. BES-II achievements are presented, and the measures taken to improve RHIC performance are described. These measures span the whole RHIC complex, including ion beam sources, injectors, beam lifetime improvements in RHIC, and operation with the world’s first bunched beam Low Energy RHIC electron Cooler (LEReC).12 MoreReceived 11 November 2021Accepted 8 April 2022DOI:https://doi.org/10.1103/PhysRevAccelBeams.25.051001Published by the American Physical Society under the terms of the Creative Commons Attribution 4.0 International license. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI.Published by the American Physical SocietyPhysics Subject Headings (PhySH)Research AreasBeam coolingBeam instabilitiesLattices in beam opticsLow-energy multiple-particle dynamicsNonlinear beam dynamicsSpace charge in beamsAccelerators & Beams

Another Unique Feature of the Nociceptor-specific Sodium Channel NaV1.9

The voltage-gated Na+ channel NaV1.9 remains an important pain target because of its preferential expression in nociceptors, its involvement in rodent models of nociception, and the association between gain- or loss-of-function NaV1.9 mutations with the presence or absence of pain in humans. At room temperature, NaV1.9 has a low threshold for activation, carrying a persistent current that may influence the resting membrane potential, action potential threshold, and burst firing. We recently demonstrated that in the absence of extracellular Na+, NaV1.9 may also contribute to a transient outward current. Here, we used whole cell patch clamp recordings of human and rat dorsal root ganglion neurons to further characterize this outward current. We found that this current had pharmacological and biophysical properties consistent with a role for NaV1.9: insensitivity to K+, Na+, Cl-, and TRP channel blockers, sensitivity to internally-administered Na+ channel blocker QX-314, and an NaV1.9-like voltage-dependence of inactivation, steady-state inactivation, recovery from inactivation. Interestingly, in contrast to TTX- and A-803467-sensitive currents, the transient outward current persisted in the absence of intracellular Na+, suggesting that NaV1.9 is less selective than other voltage-gated Na+ channels in human and rat sensory neurons. Consistent with this suggestion, the reversal potential for persistent (NaV1.9) inward currents was left shifted relative to that of TTX- and A-803467-sensitive currents. Furthermore, the outward current persisted in the presence of even relatively large intracellular cations (Cs+ and TEA+). Our results suggest NaV1.9 is a relatively non-selective ion channel. Together, these observations have at least two important implications. First, NaV1.9-mediated currents may contaminate voltage-clamp recordings of voltage-gated K+, Ca2+, and Cl- channels in sensory neurons. Second, NaV1.9 may have a more significant impact on the action potential waveform than previously appreciated, including the attenuating the action potential overshoot. Grant support from NIH R01NS122784, NIH 4UH3TR003090.

RNA Interference Via shRNA Decreases the Number of Transcripts of Scn8a in Glutamatergic Interneurons in the preBötC

Rhythmic neural activity that drives the inspiratory phase of the breathing cycle in mammals emanates from glutamatergic interneurons of the pre‐Bötzinger Complex (preBötC). A longstanding question in neuroscience is: what are the underlying mechanisms for inspiratory rhythmogenesis? One proposed mechanism involves the persistent sodium current (INaP), which is the ionic mechanism that underlies the voltage‐dependent bursting‐pacemaker activity of neurons whose rhythmic burst discharges are putatively obligatory for inspiratory rhythm generation. Specifically in brain tissues, the Scn8a gene codes for Nav1.6 ion channels, and those ion channels give rise to INaP.Different genetic tools can help us to understand the role of specific molecules in the generation of motor behaviors, such as breathing. The short hairpin RNA (shRNA) is an artificial RNA molecule, delivered through adeno‐associated virus (AAV), that can inhibit gene expression and promote long‐term knock‐down of the targeted gene.Here we administered Cre‐dependent shRNA to knock‐down Scn8a (thus Nav1.6) in Cre‐expressing glutamatergic neurons in preBötC and quantified mRNA per glutamatergic neuron. We used adult Vglut2Cre mice (10‐14 weeks old) and injected 50 nL of AAVs carrying the shRNA. We used in situ hybridization (RNAscope® Fluorescent Assay) to label mRNA of Scn8a and to identify transduced glutamatergic neurons in the preBötC for RNA quantification at 2 and 6 weeks after injection. Samples were visualized under the Four‐Channel, Upright Confocal Microscope Body Based on Cerna® System (ThorLabs), and images were acquired using the ThorImage®LS Data Acquisition Software, at 40x magnification. The amount of RNA per cell was quantified using the open‐source software Quantitative Pathology(QuPath). We obtained the mean of the number of transcripts per cell ± the standard deviation (SD), and unpaired t‐test was used to evaluate the statistical difference between the shRNA injected mice and their control group (mice whose viral vector contained a non‐targeting shRNA sequence).Our results show that at 2 weeks, the control group had 27.5±9.5 RNA/neuron, whereas the shRNA‐injected had 12.8±3.5, which represents a 53% of decrease in the number of transcripts (P<0.0001). A similar pattern was observed after 6 weeks of the injection in a different cohort, where control animals showed 35.4±6.1 RNA/cells, and the shRNA group only had 24.1±5.2, representing 32% drop in the transcripts for that group (P<0.0001). This initial protocol allowed us to verify that the gene expression has been knocked down at critical time points, encouraging further studies using shRNA to evaluate whether attenuation of Nav1.6 expression in rhythmogenic preBötC neurons impairs breathing, which could resolve the longstanding question regarding the putatively rhythmogenic role of INaP.

5‐HT2 receptor antagonism reduces human motoneuron output to antidromic activation but not to stimulation of corticospinal axons

The intrinsic electrical properties of motoneurons strongly affect motoneuron excitability to fast‐acting excitatory ionotropic inputs. Serotonin (5‐HT) is a neurochemical that alters the intrinsic properties of motoneurons, whereby animal models and in vitro experiments indicate that 5‐HT increases motoneuron excitability by activating 5‐HT2 receptors on the somato‐dendritic compartment. In the current study, we examined how antagonism of the 5‐HT2 receptor affects motoneuron excitability in humans. We hypothesised that motoneuron excitability would be reduced. The 5‐HT2 antagonist cyproheptadine was administered to 10 healthy participants in a double‐blinded, placebo‐controlled, crossover trial. Electrical cervicomedullary stimulation was used to deliver a synchronised excitatory volley to motoneurons to elicit cervicomedullary motor evoked potentials (CMEPs) in the surface electromyography (EMG) signal of the resting biceps brachii. Likewise, electrical peripheral nerve stimulation was used to generate antidromic spikes in motoneurons and cause recurrent discharges, which were recorded with surface EMG as F‐waves in a resting hand muscle. Compared with placebo, we found that 5‐HT2 antagonism reduced the amplitude and persistence of F‐waves but did not affect CMEP amplitude. 5‐HT2 antagonism also reduced maximal contraction strength. The reduced recurrent discharge of motoneurons with 5‐HT2 antagonism suggests that 5‐HT2 receptors modulate the electrical properties of the initial segment or soma to promote excitability. Conversely, as cyproheptadine did not affect motoneuron excitability to brief synaptic input, but affected maximal contractions requiring sustained input, it seems likely that the 5‐HT2‐mediated amplification of synaptic input at motoneuron dendrites is functionally significant only when excitatory input activates persistent inward currents.

Persistent Currents in Rings of Ultracold Fermionic Atoms.

We have produced persistent currents of ultracold fermionic atoms trapped in a ring, with lifetimes greater than 10sec in the strongly interacting regime. These currents remain stable well into the BCS regime at sufficiently low temperature. We drive a circulating BCS superfluid into the normal phase and back by changing the interaction strength and find that the probability for quantized superflow to reappear is remarkably insensitive to the time spent in the normal phase and the minimum interaction strength. After ruling out spontaneous current formation for our experimental conditions, we argue that the reappearance of superflow is due to weak damping of normal currents in this limit. These results establish that ultracold fermionic atoms with tunable interactions can be used to create matter-wave circuits similar to those previously created with weakly interacting bosonic atoms.

Quantum interference in asymmetric superconducting nanowire loops

Macroscopic phase coherence in superconductors enables quantum interference and phase manipulation at realistic device length scales. Numerous superconducting electronic devices are based on the modulation of the supercurrent in superconducting loops. While the overall behavior of symmetric superconducting loops has been studied, the effects of asymmetries in such devices remain under-explored and poorly understood. Here we report on an experimental and theoretical study of the flux modulation of the persistent current in a doubly connected asymmetric aluminum nanowire loop. A model considering the length and electronic cross-section asymmetries in the loop provides a quantitative account of the observations. Comparison with experiments give essential parameters such as persistent and critical currents as well as the amount of asymmetry which can provide feedback into the design of superconducting quantum devices.

Poly-dipeptides produced from C9orf72 hexanucleotide repeats cause selective motor neuron hyperexcitability in ALS

SignificanceThe GGGGCC hexanucleotide repeat expansion in the chromosome 9 open reading frame 72 (C9orf72) gene is the most common genetic cause of amyotrophic lateral sclerosis (ALS). Despite myriad studies on the toxic effects of poly-dipeptides produced from the C9orf72 repeats, the mechanisms underlying the selective hyperexcitability of motor cortex that characterizes the early stages of C9orf72 ALS patients remain elusive. Here, we show that the proline-arginine poly-dipeptides cause hyperexcitability in cortical motor neurons by increasing persistent sodium currents conducted by the Nav1.2/β4 sodium channel complex, which is highly expressed in the motor cortex. These findings provide the basis for understanding how the C9orf72 mutation causes motor neuron hyperactivation that can lead to the motor neuron death in C9orf72 ALS.

Temperature-Dependent Periodicity of the Persistent Current in Strongly Interacting Systems.

The persistent current in small isolated rings enclosing magnetic flux is the current circulating in equilibrium in the absence of an external excitation. While initially studied in superconducting and normal metals, recently, atomic persistent currents have been generated in ultracold gases spurring a new wave of theoretical investigations. Nevertheless, our understanding of the persistent currents in interacting systems is far from complete, especially at finite temperatures. Here we consider the fermionic one-dimensional Hubbard model and show that in the strong-interacting limit, the current can change its flux period and sign (diamagnetic or paramagnetic) as a function of temperature, features that cannot be explained within the single-particle or Luttinger liquid techniques. Also, the magnitude of the current can counterintuitively increase with temperature, in addition to presenting different rates of decay depending on the polarization of the system. Our work highlights the properties of the strongly interacting multicomponent systems that are missed by conventional approximation techniques, but can be important for the interpretation of experiments on persistent currents in ultracold gases.

Stabilization and control of persistent current magnets using variable inductance

Ultra-stable, tunable magnetic fields are desirable for a wide range of applications in medical imaging, electron microscopy, quantum science, and atomic physics. Superconducting magnets operated in persistent current mode, with device current flowing in a closed superconducting loop disconnected from a power source, are a common approach for applications with the most stringent requirements on temporal field stability. We present a method for active control of this persistent current by means of dynamic inductance change within the superconducting circuit. For a first realization of this general technique, we consider a variable superconducting inductor placed in series with the main magnet. The inductor acts as a dynamic flux storage device capable of transferring flux to or from the main magnet through inductance change. This allows for fine and fast adjustments of the persistent current without the use of thermal switches that limit the speed and accuracy of many present-day methods. With first experiments employing this technique, we demonstrate stabilization of a 1.95 T Nb–Ti round lens for electron microscopy against decay resulting from residual losses in the superconducting circuit, and more generally show flexibility for precise control over the magnitude and waveform of the persistent current.

Persistent current by a static non-Hermitian ratchet

We propose a scheme to generate a persistent current in driven-dissipative systems which can be described by the generalized Gross-Pitaevskii (GP) equation. Our proposal consists of fabricating a rachet-potential shape of the loss-rate profile, which simultaneously breaks the time-reversal symmetry and the parity-inversion symmetry. Unlike existing schemes to generate a current using a rachet potential in Hermitian systems, no dynamic drive is needed. The basic physics of our scheme is discussed by a simple discrete driven-dissipative GP model, and the results are also verified by a realistic continuous model. Furthermore, we demonstrate the experimental feasibility of our scheme to generate the persistent current in exciton-polariton condensates in a semiconductor microcavity.

Creep effects on the Campbell response in type-II superconductors

Applying the strong pinning formalism to the mixed state of a type II superconductor, we study the effect of thermal fluctuations (or creep) on the penetration of an ac magnetic field as quantified by the so-called Campbell length $\lambda_\textrm{C}$. Within strong pinning theory, vortices get pinned by individual defects, with the jumps in the pinning energy ($\Delta e_\mathrm{pin}$) and force ($\Delta f_\mathrm{pin}$) between bistable pinned and free states quantifying the pinning process. We find that the evolution of the Campbell length $\lambda_{\rm C}(t)$ as a function of time $t$ is the result of two competing effects, the change in the force jumps $\Delta f_\mathrm{pin}(t)$ and a change in the trapping area $S_\mathrm{trap}(t)$ of vortices; the latter describes the area around the defect where a nearby vortex gets and remains trapped. Contrary to naive expectation, we find that during the decay of the critical state in a zero-field cooled (ZFC) experiment, the Campbell length $\lambda_{\rm C}(t)$ is usually nonmonotonic, first decreasing with time $t$ and then increasing for long waiting times. Field cooled (FC) experiments exhibit hysteretic effects in $\lambda_\textrm{C}$; relaxation then turns out to be predominantly monotonic, but its magnitude and direction depends on the specific phase of the cooling--heating cycle. Furthermore, when approaching equilibrium, the Campbell length relaxes to a finite value, different from the persistent current which vanishes at long waiting times $t$, e.g., above the irreversibility line. Finally, measuring the Campbell length $\lambda_\textrm{C}(t)$ for different states, zero-field cooled, field cooled, and relaxed, as a function of different waiting times $t$ and temperatures $T,$ allows to spectroscopyse the pinning potential of the defects.

Cholinergic modulation of persistent inward currents is mediated by activating muscarinic receptors of serotonergic neurons in the brainstem of ePet-EYFP mice.

Persistent inward currents (PICs) play important roles in regulating neural excitability. Results from our previous studies showed that serotonergic (5-HT) neurons of the brainstem expressed PICs. However, little is known about cholinergic (ACh) modulation of PICs in the 5-HT neurons. The whole-cell patch-clamp recordings were performed in the brainstem slices of ePet-EYFP mice to investigate the electrophysiological properties of PICs with cholinergic modulation. PICs in 5-HT neurons were activated at -51.4 ± 3.7mV with the amplitude of -171.6 ± 48.9 pA (n = 71). Bath application of 20-25μM ACh increased the amplitude by 79.1 ± 42.5 pA (n = 23, p < 0.001) and hyperpolarized the onset voltage by 2.2 ± 2.7mV (n = 23, p < 0.01) and half-maximal activation by 3.6 ± 2.7mV (n = 6, p < 0.01). Muscarine mimicked the effects of ACh on PICs, while bath application of nicotine (15-20μM) did not induce substantial change in the PICs (n = 9). Muscarine enhanced the amplitude of PICs by 100.0 ± 27.4 pA (n = 28, p < 0.001) and lowered the onset voltage by 2.8 ± 1.2mV (n = 28, p < 0.001) and the half-maximal activation by 2.9 ± 1.4mV. ACh-induced increase of amplitude and hyperpolarization of onset voltage were blocked by 3-5μM atropine. Furthermore, the muscarine-induced enhancement of the PICs was antagonized by 5μM 4-DAMP, the antagonist of M3 receptor, while the antagonists of M1 (Telenzepine, 5μM) and M5 (VU6008667, 5μM) receptors did not significantly affect the PIC enhancement. This study suggested that ACh potentiated PICs in 5-HT neurons of the brainstem by activating muscarinic M3 receptor.