Persistent Corpus Luteum Research Articles

Effect of PG-600 dose in cows with persistent corpus luteum treated with PGF2α and hCG

The purpose of this study was to determine the effect of administering a dose of gonadotropin (PG-600) in cows with persistent corpus luteum (PCL) which were treated with prostaglandin F2 α (PGF2α) and human chorionic gonadotropin (hCG). This study used 20, three-year-old cows diagnosed with PCL, had recently given birth, and were not pregnant. Cows were randomly allocated into groups T0, T1, T2 and T3. Cows in all groups were injected with PGF2α (25 mg/cow). Simultaneously, cows in groups T1, T2 and T3 were also injected with PG-600 at doses of 100, 150 and 200 IU/cow respectively. PGF2α and PG-600 were injected when PCL was identified. After that, cows showing signs of estrus were injected with hCG (100 IU/cow) and artificially inseminated 12-18 hours after the appearance of signs of estrus. AI was conducted using frozen thawed semen. Sixty days following AI, a pregnancy examination was conducted using USG. Estrus was detected in all treated cows, while the pregnancy rates varied. Compared with other groups, the groups given 100 and 150 IU PG-600 produced higher pregnancy rates. It could be concluded that the combination of 25 mg PGF2α, 150 IU PG-600, and 100 IU hCG was the most effective treatment to produce high pregnancy rates in cows with persistent corpus luteum.

RESEARCH ON THE DEVELOPMENT AND THERAPY OF PERSISTENT CORPUS LUTEUM IN COWS

"Pathological puerperium can mainly cause significant economic damage through the high losses that accumulate throughout the herd and especially through the high percentage of sterility and infecundity. According to literature data, it is shown that this infertility ratio can range quite widely between 5.0-30.0% of the total bull herd. A criterion in the occurrence of this gynecological disease is the feed, which can vary in terms of quantity and quality, the farming regime to which the cows are subjected and not especially environmental factors (lack of body hygiene, low temperatures during the winter season and prolonged housing). In both research years, the incidence of cows diagnosed with persistent corpus luteum was high at 7.1% in 2021 and 9.9% in 2022 in the non-lactating cows group and after a period of 90 days after parturition. In 2021 the persistent luteal corpus luteum (CLP) in April did not register any cases, the most significant values were recorded in the months: October (1.9%), November (2.5%), December (3.5%) and the highest level of (3.9%) being reached in January. In 2022 the recorded values of persistent corpus luteum were close compared to the first year of investigation, peaking in January at 3.1%. According to the number of lactations, in 2021, 19.3% females, in 1st lactation only 1.5% were diagnosed with this gynecological condition. Also, the highest level of 4.4% was recorded in cows in their 6th lactation and above."

The marked dextro-dominance causes accentuated morphophysiological variations in the female reproductive organs of the bat Molossus molossus

Molossus molossus is an insectivorous molossid bat that is important in the control of nocturnal insects. It is the nominal and the most representative species of the family. However, there are few studies about its reproduction. Thus, this study aimed to evaluate variations of its female reproductive organs during the different reproductive phases. Twenty adult females, divided into four sample groups (non-reproductive, early and advanced pregnancy and lactation), were submitted to morphological and morphometric analyses. Results show that the female reproductive system of M. molossus is composed of ovaries, a short bicornuate uterus, slightly convoluted uterine tubes and vagina. The system presents a distinct morphofunctional asymmetry, with a marked dextro-dominance. The right ovaries of all analyzed groups (NON, P1, P2, and LAC) showed follicles at different stages of development, a large number of interstitial glands and a small, but persistent corpus luteum. Ovulation is simple, unilateral and preferential, occurring exclusively in the right ovary. Follicular development in the left ovary usually does not pass the secondary stage. Implantation is fundic and preferential, occurring exclusively in the right uterine horn. The placenta is formed with two distinct chorioallantoic portions, one diffuse endotheliochorial, which covers the entire uterine cavity and regresses in the final stages of pregnancy, and the principal discoidal hemochorial portion, formed in the implantation site. The uterine cervix presents a pseudostratified epithelium, while the vagina has a little keratinized stratified epithelium, which does not accentually vary in the different reproductive stages, but can disrupt and shed in some cases.

The course of the postpartum period in cows in the presence of concomitant pathology

The decrease in productivity in cows is directly related to the pathologies of the organs of the reproductive system that occur at the end of the transit period, therefore it is important to improve the methods of diagnosis and preventive therapy of cows in this period. The purpose of the work was to establish the forms of pathologies that occur in the transit period in cows, their interrelationship and the causes that cause them in terms of seasonal dynamics. When conducting research, morphological, clinical (examination, palpation), laboratory (bacteriological studies of the uterus), and statistical (statistical reliability) methods were used. The research was conducted in the limited liability company “Milk of the Fatherland” in 2 departments where unattached housing is used. The occurrence of subinvolution of the uterus against the background of litter retention and persistent corpus luteum was established by 9.5% more in the 1st department. A 43.5% correlation of the prevalence of delayed involution with endometritis was revealed. An increase, 12.8% in January and up to 14.1 in February, of cases of subinvolution of the uterus was established, while in May the similar indicator was 5.0%. The prevalence of persistent corpus luteum was established up to 16.8%, luteal cyst – up to 1.7%, ovarian follicular cyst – 2.1%. The presence of microflora in the uterine cavity (E. coli, Ps. aeruginosa and S. aureus – 49.8% S. aureus and E. coli – 33.5%, Pr. vulgaris and E. coli – 16.7%) provoked the occurrence inflammatory processes: chronic endometritis – 10.4%, vulvovaginitis – 18.1%, cervicitis – 21.4%. Research results can be used to develop new and improve existing methods of treatment of cows with pathology of genital organs in the transit period in farms of different forms of ownership

Evaluation of the effectiveness of treatment of cows with persistent ovarian corpus luteum

The article presents the results of studies on the use of the drug regostenol in persistent ovarian corpus luteum. The studies were carried out in JSC "PZ" Meliorator "of the Saratov region, where they noted the widespread obstetric and gynecological pathology in cows. Among these diseases, 43.91% are functional disorders of the ovaries. Persistent corpus luteum was recorded in 12.69% of animals. After the use of regostenol, 91.67% of cows showed the stage of excitation of the sexual cycle, and the time for the onset of the first sexual cycle came in 5.2 ± 0.03 days. In the control group of cows, no manifestations of sexual cyclicity occurred. Experimental studies have shown that after the use of regostenol in cows with a persistent corpus luteum, 75.0% of animals became pregnant with an insemination index of 1.8. Whereas in the control group, all animals remained infertile. The data obtained indicate the expediency of using the drug, since it allows achieving the manifestation of sexual cyclicity after prolonged anaphradesia and fertilization of most of the cows after insemination.

Імунобіологічна реактивність організму корів за дисфункції яєчників

It is known that the immune status of the female organism changes dynamically at all stages of both the physiological and pathological state of the reproductive system. The work presents the results of scientific research and analysis of immunological studies in cows with ovarian dysfunction. We studied the nature and peculiarities of the relationships between the indicators of immunity and sexual function of infertile cows before and after treatment. The purpose of the research was to find out the dynamics of some morphological and biochemical blood indicators and the immune status of the body in cows with functional disorders of the ovaries. Certain regularities of the immune status in cows with persistent corpus luteum of the ovary and hypofunction of the ovaries have been established. The results of our research confirmed and supplemented previously established data on changes in hematological and immunological indicators in cows with ovarian pathology. It was established that the development of this pathology was accompanied by an immunodeficient state of the body, disorders of the cellular and humoral links of immunity, and a decrease in indicators of non-specific protection. Ovarian dysfunction in cows mainly develops against the background of immunosuppression and deficiency of indicators of cellular protection of the body, which was supplemented by a decrease in the content of erythrocytes, hemoglobin and total protein in the blood. A significant increase in the content of “0” mononuclear cells in the blood of sick cows indicates a violation of the mechanisms of lymphocyte differentiation, which negatively affects the formation of the body’s immune response. The imbalance of individual lymphocyte populations indicates the need for immunocorrection in order to increase the content of B and T cells, which will, accordingly, lead to a decrease in the share of low-active “0”-lymphocytes, and will contribute to the restoration of the immune defense mechanisms of the cows’ body. The decrease in indicators of individual factors of immunobiological reactivity of the body of cows with gonadal pathology substantiates the need for the use of general stimulating drugs with an immunomodulating effect.

Early diagnosis of pseudopregnancy in goats by ultrasonography and response to treatment using cloprostenol sodium

Pseudopregnancy is a state of temporary infertility in goats characterised by the accumulation of sterile fluid in the uterus in the absence of a foetus with persistent corpus luteum. B-mode ultrasonographic examination of goats on day 60 post-breeding was conducted for differential diagnosis of pregnancy, non-pregnancy and pseudopregnancy. Pseudopregnant does diagnosed on day 60 (day 0) post-breeding (Group I, n=8) were treated with three doses of 125μg of cloprostenol sodium (i/m) 10 days apart and were bred by natural service on induced oestrus after the third dose of treatment. The response to treatment was evaluated and compared with normal cycling does (Group II, n=8) which were mated on spontaneous oestrus. The uterine wall thickness measured ultrasonographically on day 0 in group I and on day 60 post-breeding in pregnant does of group II were 0.63 ± 0.02 and 0.66±0.03 cm, respectively (P >0.05). Serum progesterone concentration estimated on day 0, day of the evacuation of uterine fluid, days 10 and 20 in group I and on day 60 post-breeding in group II were 2.73 ± 0.19, 0.78± 0.19, 1.67 ± 0.10, 1.72 ± 0.19 and 3.64± 0.16 ng/mL, respectively. A statistically significant difference was observed between day 0 in group I and day 60 in group II; within group I, between day 0 and the day of evacuation and between the day of evacuation and day 10. Pregnancy diagnosis on day 60 post-breeding revealed a conception rate of 50 and 75 per cent in groups I and II, respectively (P>0.05).

Factors Associated with the Odds of Pregnancy for Dairy Cattle after Treatment of Ovarian Disorders in Northern Vietnam

The study was carried out to assess factors related to the odds of pregnancy for dairy cattle after treating ovarian disorders in some Northern Vietnamese provinces. A total of 449 ovarian-disordered heifers and cows were given appropriate treatments for their diseases and were diagnosed by rectal palpation with pregnant status after artificial insemination. Data collected from ruminants were analyzed by applying Binary Logistics Procedure in SAS 9.0 and three methods were utilized to test the significant level of regressive coefficients related to the factors, which included various nutritional, environmental, and management factors (locations, parities, ages, body condition scores, housing types, mineral licking block diets, rubber bedding, and categories of disordered ovaries). Based on the odds ratio, cows and heifers in Ha-Noi zone had higher odds of pregnancy than in Vinh-Phuc province (odds ratio [OR] =1.97, p<0.05). Multiparous cows were likely to get a lower chance to conceive than heifers. The odds of pregnancy for dairy cattle decreased with the increase of age, and with each month increased of age, the odds of pregnancy decreased by 1.8%. The better housing condition the dairy cattle live in, the higher pregnancy rate they get. The pregnant opportunity of the dairy cattle kept in the concrete floor house is higher than that kept in rubber floor house (OR=4.03; p<0.0001). Adding mineral licking block in their diet affected the conception odds but did not affect BCS. When the appropriate treatment was used, dairy cattle with inactive ovaries were less likely to conceive than those with cystic ovarian disease and persistent corpus luteum.

Ultrasonographical and hormonal comparative between true and postpartum anestrus of cows in south of Iraq

Anestrus is one of the major reproductive problems. A total of 218 local breed cows were diagnosed as 105 cows with true anestrus and 113 cows with postpartum anestrus. The results indicated that most cases of postpartum anestrus were concentrated from December to May, while the true anestrus occurred from May to September. The most important causes that were diagnosed by ultrasound for true anestrus cases were inactivity of the ovaries and anovulatory follicles. A high percentage of postpartum anestrus cases were suffered from luteinizing cysts, ovarian inactivity. Hormonal assay for inactive ovaries in true and postpartum anestrus cases revealed a significant decrease (p&lt;0.05) in the levels of estrogen, progesterone, FSH and LH. While the hormonal results of postpartum anestrus cases revealed a significant decrease (p&lt;0.05) in the estrogen with a significant increase (p&lt;0.05) in the progesterone in case of luteal cyst, persistent corpus luteum and cystic corpus luteum which accompanied by a significant increase (p&lt;0.05) in the prolactin .The ultrasonographical features for true anestrus showed an increase in echo rates for ovaries. In postpartum anestrus cases the luteal cyst revealed 2.88-2.90cm follicle like structure. While the cases of persistent corpus luteum showed 1.15cm-1.75cm dense theca tissue.

Characterizing zoo-housed Bactrian camel (Camelus bactrianus) reproduction using gonadal steroid metabolite analysis in feces

Millions of people globally depend on camelids, which demands an increased knowledge of their reproduction. We used zoo-housed Bactrian camels (Camelus bactrianus) to better understand camelid reproductive physiology. Our specific objectives were to: 1) validate the use of fecal hormone metabolite analysis to characterize camel reproductive physiology during sexual maturity and pregnancy; and 2) determine the influence of season on male and female reproduction. We collected fecal samples from 1 male and 3 females housed at Lincoln Park Zoo (Chicago, IL, USA) 1 to 2 times per week for 3.5 years. Extracted hormones were analyzed using enzyme immunoassays for progestogen (FPM), estrogen (FEM), and androgen (FAM) metabolite concentrations. One female sexually matured during our study as evidenced by increased FEM baseline. Results demonstrated seasonal effects on male androgen production with FAMs higher (P < 0.05) January to June (mean ± SEM: 664.6 ± 22.6 ng/g wet feces), compared to July to December (401.6 ± 17.5 ng/g wet feces). One female experienced a persistent corpus luteum, a reproductive abnormality, which was identified by prolonged elevated FPM. FPMs increased during pregnancy for two females (452.9 ± 24.9 and 294.4 ± 19.8 ng/g wet feces) with a gestation of 404 d and 442 d, respectively. The third female never conceived. The FEMs varied (P < 0.05) during the year with no clear seasonal patterns (monthly mean range: 213.1–371.0 ng/g wet feces). Fecal hormone metabolite analysis is a validated method for assessing male seasonality and female pregnancy in the Bactrian camel and can for their management and conservation in zoos and the wild.

Immunocontraception of male and female giraffes using the GnRH vaccine Improvac®.

The aim of this study was to develop protocols for contraception in both sexes of giraffes (Giraffa camelopardalis) by using the GnRH vaccine Improvac®. We evaluated the success of immunization by analyzing fecal reproductive hormone metabolites in female (n = 20) and male (n = 9) giraffes. Endocrine analysis provided the basis for the successful immunization protocol, as well as for assessing long‐term effects. Reliable reduction of fecal steroid metabolites to baseline levels in female giraffes was achieved with three, and in males with four or five injections at 4‐week intervals. Effective booster injections were administered at 2‐month intervals in the first year of treatment and at three to 4‐month intervals in the following years. In addition to endocrine analysis, we determined vaccination efficacy in bulls by assessing testicular atrophy. Long‐term (>2 years) use in females was often accompanied by prolonged periods of persistent corpus luteum activity, although normal cycles were not observed. Problems might occur with reversibility, because in a few males and females, even after more than 2 years since treatment had been stopped, fecal hormone metabolites have not returned to pretreatment levels. The results are somewhat ambiguous, as reproduction can be suppressed by use of Improvac®, but the question of reversibility remains unsolved.

Fertility restoration of racing mare with persistent corpus luteum.

Background and Aim:Persistent corpus luteum (PCL) causes anestrus in mares. This study aimed to determine the effect of intrauterine prostaglandin F2α (PGF2α) treatment on PCL of racing mares to restore fertility.Materials and Methods:Twelve racing mares suspected with PCL were diagnosed using transrectal palpation and confirmed by serum progesterone (P4) concentration measurement. PGF2α was infused intrauterine, followed by serum collection at 24, 48, and 72 h after. Estrous symptoms were monitored, and mating was conducted on day 3 of estrus with an earlier injection of 8.4 μg gonadotropin-releasing hormone twice a day. Transrectal palpation was performed on days 21-30 to observe the corpus luteum. Pregnancy diagnosis was performed rectally on 40-45 days post-mating and confirmed using Doppler ultrasound scanning.Results:Eleven of the 12 mares had PCL. There was a dramatic reduction in the P4 concentration following PGF2α treatment of mares with PCL. All mares exhibited estrus 2.6±0.55 days post-treatment with a P4 concentration of 0.12±0.12 ng/mL. Rectal palpation and P4 concentration on 21-30 days after estrous onset showed that all mares were ovulating. The evaluation of P4 concentration on days 40-45 post-mating showed that all mares were still in the luteal phase. However, the pregnancy rate was only 54.5% based on rectal palpation and Doppler ultrasound scanning.Conclusion:Treatment of PCL in racing mares with an intrauterine infusion of PGF2α restored the estrous cycle and induced ovulation and pregnancy.

Angka kebuntingan sapi perah dengan korpus luteum persisten setelah pemberian kombinasi prostaglandin F2α dan gonadotropin

This case study aims to determine the effect of a combination of prostaglandin F2α (PGF2α) with Gonadotropin (PG-600), and human chorionic gonadotropin (hCG) on the estrus and pregnancy rates in Holstein Friesian (HF) cows with persistent corpus luteum (PCL). A total of 15 Holstein Friesian (HF) dairy cows aged 3-5 years with body condition score (BCS) 5-7 (scale 1-9) diagnosed with PCL, were injected with 7.5 mg PGF2α intra-vulvar submucosa. Cows were then divided into three treatment groups. Group P0 was not given the combination treatment, while P1 group was injected with a combination of 300 IU PG-600 and 150 IU hCG, meanwhile P2 group was was injected with a combination of 300 IU PG-600 and 300 IU hCG intra-muscularly. The PGF2α and PG-600 injections were carried out on the same day PCL was diagnosed. Meanwhile, the injection of hCG was carried out at the same time as the AI. The Pregnancy examination was performed 90 days after AI through rectal palpation. The results showed that all treated cows were estrus observed with the fastest onset of estrus was the P2 group, that was significantly different (p &lt;0.05) compared to the P0 and P1 groups. All treated cows were diagnosed as pregnant. It can be concluded that the administration of PGF2α or its combination with gonadotropins in HF cows with PCL could result in 100% estrus and pregnancy rates.

Some Factors Associated with Ovarian Disorders of Dairy Cattle in Northern Vietnam

This research’s objectives were to assess the risk factors associated with ovarian disorders in dairy cattle in Hanoi Capital, Ha Nam Province, Vinh Phuc Province, and Moc Chau District in Son La Province. The ovarian diseases were evaluated by rectal palpation combined with information collected based on the questionnaire and surveys. Records of feeding management and ovarian disorders were comprising of persistent corpus luteum (PCL), cystic ovarian disease (COD), inactive ovary (IO) from 818 females, including heifers and cows from the first to the eighth parities. A multinomial logistic regression procedure in SAS9.0 was applied for data analysis. The results have shown that the changes of region, age classes, housing condition, and body condition scores were associated with the odds of suffering from dairy cattle’s ovarian disorder categories. The cows got the odds of suffering from PCL in the old age or fatter condition, and IO at the younger or angular cows. When cattle were confined in simple houses or laid on rubber bedding, they suffered from IO, but they tended to get the odds of suffering from PCL when the cattle were raised in modern houses or laid on concrete bedding. In conclusion, region, parity, body condition score, housing type, and bedding material affect ovarian disorders in dairy cattle in northern Vietnam.

NEW EFFECTIVE METHODS OF TREATMENT OF PERSISTENT INFERTILITY IN COWS (CORPUSLUTEUMPERSISTENS)

This article examines the persistent corpus luteum, the causes of infertility among cows, quantitative levels of steroid hormones in the serum of experimental cows, experimental cows were fertilized twice at 12-hour intervals using the manoservical method, and 3 months after insemination, cows were examined by commission-rectal studies. The application of symptomatic and operative therapies in the treatment of persistent corpus luteum, and the results of experiments, discussions were noted.

Cytology of smears of prints with symptomatic forms of infertility in cows

Many works of domestic and foreign scientists are devoted to the study of etiological factors, pathogenesis, as well as the development of effective methods of diagnosis and therapy for symptomatic infertility of cows, however, insufficient attention is paid to cytological examination of the genital organs.So the goal of our work was to study the cytology of vaginal mucus at the symptomatic form of infertility of cows. The article presents the cytological composition of vaginal mucus from the mucous membrane of the caudal section of the cervix, the lateral surface of the vagina and the dorsal wall of the vaginal vestibule. Research was carried out with 5 groups of cows 4–5 years old cows 6 animals in each, weighing 500–600 kg, with an average productivity of 7500 kg of milk per lactation. The tests were cows were on the 18–24 day after insemination and during the sexual cycle braking, research of the joint course of uterus and hypotension of the ovarian, the presence of persistent corpus luteum and subclinical chronic endometritis, cysts and persistent corpus luteum of the ovarian body. For sub-clinical chronic endometritis, basal cells (32–41 %) are found in wet prep with vaginal mucosa, which are arranged in layers, parabasal (22–6 %) and superficial (5–21 %), but more interstitial (37–66 %), and bare neutrophil nuclei. According to hypofunction of ovaries in vaginal smears, neutrophils, vacuoles in interstitial cell nuclei (44–56 %), basophilic cells (30–43 %), superficial cells (20–21 %), parabasal cells (3–6 %). Basal cell hypofusions were (3–43%), parabasal (3–6 %), interstitial (44–56 %), superficial (20–21 %), and bilateral (12–26 %) (4–5 %) (36–55 %) and (30–33 %). It should be noted that for the functional state of the body, the cells from the cervix are flat. In addition, the drug contains single white blood cells with light cytoplasm without signs of phagocytosis. They are structurally similar to cells of vaginal smear. During the esrtus, the smear is dominated by interstitial and surface cells and white cells, and in the follicular proliferative phase, the number of surface cells increases, while the ovulator phase is characterized by the pronounced maturation of the cells, they are flat and spaced apart.

Morphophysiological variations of the female reproductive organs of the vespertilionid bat Myotis nigricans during its different reproductive phases

Myotis nigricans is an important species of insectivorous bat from the Vespertilionidae family, which is endemic of the Neotropics. Despite its wide distribution, no detailed study of the reproduction is found for this species. Thus, the aim of the present study was to evaluate and characterize morphological and morphometric variations of the female reproductive organs of M. nigricans during its different reproductive phases. Twenty adult females were collected, divided into four (4) sample groups, according to the reproductive status (nonreproductive, initial and advanced pregnancy and lactating), and submitted to anatomical, histological and morphometric analyses. The results demonstrated that the female reproductive organs of M. nigricans were composed of the ovaries, the uterus, the uterine tubes and the vagina, in addition to the mammary glands. The ovaries present a great number of interstitial glands and a small but persistent corpus luteum. Ovulation is simple, unilateral and non-preferential, possibly alternated in successive ovulations and may be sequential with the occurrence of postpartum estrus. The uterus is bicornuate, implantation is fundic and the placenta is chorioallantoic, discoidal and hemochorial. The vaginal epithelium is weakly keratinized and shows great disorganization during the periods of pregnancy and lactation.

A randomized controlled clinical trial on the effect of acupuncture therapy in dairy cows affected by pyometra

Pyometra (PYO) is a reproductive disease characterized by the accumulation of purulent or mucopurulent material within the uterine lumen, in the presence of an active corpus luteum (CL). As the CL continues secreting progesterone, PYO would develop following endometritis. Due to prohibited use of artificial luteolytic hormones in US certified organic dairies, conventional therapies consisting of administration of prostaglandin F2α, are not applicable. The objective of this study was to evaluate the efficacy of two acupuncture procedures on the treatment of persistent CL in cows with PYO. We hypothesized that acupuncture would reduce CL diameter and serum progesterone (P4) concentrations, leading to regression of PYO. Holstein cows with PYO, at an USDA certified organic dairy farm in Northern Colorado, were enrolled in a randomized controlled clinical trial and assigned to 1 of 3 treatments: (1) control pyometra (CP; no treatment; n = 17); (2) electroacupuncture (EAP; n = 15); and (3) laser acupuncture (LAP; n = 15). Each cow received three 9-min (EAP) or 20-min (LAP) acupuncture sessions in alternate days. All study cows had blood samples collected for determination of serum progesterone concentration at d0, and at d2, d4, d11, d18, and d25 after first treatment. The ovaries were scanned by transrectal ultrasonography at -3d, d0, d2, d4, d11, and d18 to determine the diameter of the CL. The effect of treatment in the outcome variables was evaluated by ANOVA and by repeated measures analyses, accounting for baseline data (CL diameter and progesterone serum concentration). Average ± SE change in CL diameter from d0 to d18 were 0.94 ± 1.0 mm, 0 ± 1.0 mm, and − 0.33 ± 1.0 mm for CP, EAP, and LAP, respectively. The repeated measures analysis indicated no significant differences for CL diameter among groups. None of the study cows had serum progesterone values <1 ng/ml by the end of the monitoring period (d25) and average ± SE change from d0 to d25 were − 4.0 ± 1.97 ng/ml, −0.76 ± 2.1 ng/ml, and 3.24 ± 1.9 ng/ml for CP, EAP, and LAP, respectively. The repeated measures analysis indicated no significant differences for serum progesterone concentrations among groups. On farm cow records reviewed 150 d after treatment indicated that 2 cows in EAP and 1 cow in LAP conceived 38 d, 68 d, and 38 d, after treatment completion. In conclusion, acupuncture was not an effective treatment for persistent CL in cows with PYO, during the monitoring period.

Pregnancy Rate and Reproductive Disorders Examination of Inseminated Brahman Cross Cows by Rectal Palpation and Ultrasonography

Pregnancy is a condition when a fetus began to develop in the uterus of the cow. Early pregnancy detection becomes important as it would determine the success of breeding management as well as to achieve the best productivity. This study aimed to evaluate and observe the pregnancy rate and reproductive disorders of Brahman Cross (BX) cows and compare the results to the Ultrasonography (USG) observation. The materials of the study were 80 BX cows selected through a purposive sampling method with the following criteria: aged 2-3 years, weighed 300-500 kg, has at least calved once and had the body condition score (BCS) at 4-7 (in 1-9 scale). This study was conducted as experimental research, with 40 BX cows were artificially inseminated with Ongole crossbred (PO) semen at 0-2 h interval and the other 40 BX cows were at 8-10 h interval from the estrous detection. All of the BX cows were firstly synchronized with PGF2α (LUTALYSE) and then inseminated with PO semen (>40% motility) as according to the Indonesian standard. The pregnancy rate of the BX cows was observed through rectal palpation and 17β-estradiol hormone observation and further examined for the reproductive disorders by using USG. The obtained data were then analyzed descriptively and the results showed that 0-2 h insemination had a higher success of pregnancy (32.5%) compared to the 8-10 h interval (27.5%). Moreover, the highest 17β-estradiol in the inseminated BX cows was at 5,765 ng/ml, while the lowest was at is 0.41 ng/ml. The USG observation showed that 6 BX cows (7%) in this study had a follicular cyst, 2 BX cows (2.25%) had persistent corpus luteum and 1 BX cows (1.25%) had ovarian hypofunction.

Fetal Mummification in Domestic Animals: A Critical Review

Fetal mummification is the unusual and most important gestational disorder in domestic animals with the highest incidence in multiparous and polytocous species like swine (up to 4%). The fetal mummification is different in monotocous and polytocous species. In cattle and buffalo, it is commonly observed between 4th to 8th month of gestation. The fetal death without abortion, persistent corpus luteum, and progesterone influence collectively lead to the formation of the mummified fetus. The two notable forms of mummification in domestic animals include haematic and papyraceous types. The etiology is multifactorial and different conditions are held responsible for the occurrence of fetal mummification in each species. The common causes include genetic or chromosomal abnormalities, placental defects, infectious agents, abnormal hormonal concentrations, and drugs. Transrectal palpation, radiography, and ultrasonography are useful clinical tools for the diagnosis of mummification. The treatment protocols and management strategies differ between the species. The prevention of fetal mummification is possible by practicing good hygiene and sanitation, periodically following the standard vaccination protocols, and adopting the recommended reproductive monitoring programmes.