Avocado Research Articles

Improved beekeeping practices, honey bee flora potential and flowering calendar in South Ethiopia.

In Ethiopia, improved hive technology dissemination was started before five-decades. However, the adoption of improved beekeeping technology is still very low. This study was conducted with the main objectives to evaluating improved beekeeping adoption level and honey yields of different hives and identification of major honey bee plants and flora calendar in the Gedeo zone, South Ethiopia. Three districts were selected purposively based on beekeeping potential and the number of improved hives own by beekeepers. The data was collected from 180 respondents using cross-sectional survey. The data was analyzed by using descriptive statistics such as mean, frequency and percentage and ANOVA. The result shown that the compositions of disseminated hives in the entire sampled respondents were 286, 476, 121 and 1494 Zander hive, Kenyan top bar hive (KTBH), Mud/Ethio-Ribrab hive (ERH) and Traditional hives respectively. Traditional beekeeping was the dominant system with 63% and intermediate followed by 25%, while modern beekeeping was only 12%. Based on overall mean honey yield, there was no significant difference (P = 0.244) between Zander and KTBH. However, the average honey yield of these improved hives were significantly (P<0.05) higher than Mud/ERH and Traditional hives. Gedeo zone had rich floral resource and diverse floral calendar. Hygenia abyssinica, Bidens ghedoensis, Erythrinia abyssinica, Eucalyptus species, Cordia africana, Coffee arabica, Vernonia species, Susbania susban and Persea americana were major honey bee flora in Gedeo zone. February-March was major honey harvesting season while May-July and October-December respectively were minor honey harvesting periods. Nevertheless, the majority of beekeepers have been practicing honey harvesting once a year from all hives due to lack of awareness and practical skills. Therefore, we recommend that the local government should focus on educating beekeepers to enable them utilizing exhaustively the opportunities of multi-floral season and improved hive technology to maximize honey yield in the area.

Discovery of a Biocontrol Strain Trichaptum laricinum: Its Metabolites and Antifungal Activity against Pathogenic Fungus Colletotrichum anthrisci.

Finding safe and environmentally friendly fungicides is one of the important strategies in modern pesticide research and development. In this work, the antipathogenic effects of the fungus Trichaptum laricinum against the anthracnose pathogen Colletotrichum anthrisci were studied. The EtOAc extract of T. laricinum showed remarkable antifungal activity against C. anthrisci with an inhibition rate of 50% at 256 μg/mL. Bioguided isolation of the cultural broth of T. laricinum produced four new drimane sesquiterpenes, trichalarins A-D (1-4), and six other metabolites (5-10). Their structures were established by extensive spectroscopic methods, quantum chemical calculations, and single-crystal X-ray diffraction. All compounds exhibited antifungal activity against C. anthrisci with minimum inhibitory concentrations (MICs) of 8-64 μg/mL in vitro. Further in vivo assay suggested that compounds 2, 6, and 9 could significantly inhibit C. anthrisci growth in avocado fruit with inhibition rates close to 80% at the concentration of 256 μg/mL, while compounds 2 and 6 had an inhibition rate over 90% at the concentration of 512 μg/mL. The EtOAc extract of T. laricinum had no inhibitory effect on Pinus massoniana seed germination and growth at the concentration of 2 mg/mL, showing good environmental friendliness. Thus, the fungus T. laricinum could be considered as an ideal biocontrol strain, and its metabolites provided a diverse material basis for the antibiotic agents.

Study of the Physical, Chemical, and Biologically Active Properties of Avocado Pulp (Persea americana), and Its Use in the Preparation of Some Functional Dairy Products

This research conducted with the aim of manufacturing healthy products (yoghurt and local cream cheese) rich in bioactive compounds and healthy fatty acid using avocado fruit pulp. Avocado pulp used in the manufacture of yoghurt at two, four, and six percent, and the yoghurt stored at 4ﹾ C for seven days. Local cream cheese was handmade by replacing animal butter with 15% avocado pulp. The content of phenolic compounds in fresh avocado pulp determined using HPLC, where it was found to be rich in kaempferol 30.88 ppm, ferulic acid 30.25 ppm, apigenin 22.58 ppm, quercetin 18.98 ppm, and P-coumaric acid 12.66 ppm, as well as rich in some fatty acids: oleic 59.58%, palmitic 17.58%, and α-linolenic 18.05%. Compared to the control sample at the first day of storage, the avocado-fortified yoghurt led to an increase in antioxidant activity by 10.86%, 25.12%, and 34.63%, and in total phenolic content by 129.55%, 343.18%, and 404.55%. The yoghurt sample at four percent was the most accepted in texture, taste, flavor, and general acceptance, and the avocado added a buttery, astringent, and distinct taste to the avocado-fortified samples. Also, the manufactured of local cream cheese by avocado led to a decrease in moisture and fat, and an increase in acidity and total solids, and it was possible to obtain a low-fat functional food with high nutritional value. Results showed that supporting yoghurt with avocado had a positive effect on reducing the total number of microorganisms and was free of molds, yeasts, and coliform growths during storage.

CAN THE APPLICATION OF AGROFORESTRY AT MONOCULTURAL FOOD CROP AREAS SUPPORT MANUFACTURER CHEMICAL FERTILIZER REDUCTION PROGRAMS WITHOUT SACRIFICING FARMERS' INCOME?

In an agroforestry system, nutrients from the subsoil can be made available to food plants through the absorption process by trees with deep roots. However, shade from canopy tree plants can suppress photosynthesis and reduce food crop production and farmer income [INCM]. This antagonism needs to be evaluated when introducing agroforestry programs into monoculture food crop areas to reduce manufactured chemical fertilizers application. This research was carried out from June to August 2023 at Natar District, South Lampung Regency to determine the effect of tree strata (teak, acacia, and albizia), multi-proposed tree species or MPTS (Parkia speciosa, avocado, and jack fruit), shrubs (coffee and cacao), and ground cover (vegetables, peanut, and soybean) to [INCM]. Interviewing forty respondents who were still retaining one or more of these tree strata among food crops including upland rice, corn, and cassava. The OLS (ordinary least square) model was employed at 90% confidence level. To eliminate the model error, the variables ownership of productive assets (upland, cattle, sheep, and cock), fertilizer application (urea, ponska, KCl and liquid fertilizer), compost, and pesticide use were also incorporated in the model. The results suggest the significant effects: (1) that positive ones are acacia, teak, vegetables, peanuts, and, soybeans, respectively USD 86.4 (P=0.000); 88.3 (P=0.000); 109,5 (P=0.000); 114.6 (P =0.000); 145.1 (P=0.000); and (2) which negative were Albizia falcataria, Parkia speciosa, avocado, coffee, and cacao respectively of around USD 31,7 (P=0.002); 57.1 (P=0.000); 43.2 (P=0.003); 62,0 (P=0.000) and 96.37 (P=0.000).

Statistical physics analysis of the adsorption of reactive red 141 dye on residual avocado peel (Persea americana) chemically modified with H2SO4 and HNO3

The preparation and adsorption properties of avocado peels treated with H2SO4 and HNO3 for the removal of reactive red 141 dye are reported in this manuscript. Equilibrium adsorption of this organic dye on the two adsorbents was experimentally quantified at pH 6.5 and 298.15–328.15 K. An advanced physical model that assumed the formation of a finite number of layers of the adsorbed pollutant molecules on the adsorption sites was used to estimate the steric and energetic parameters of the dye adsorption mechanism. The modeling results showed dye molecular aggregation, in which each adsorption site of avocado peels treated with H2SO4 and HNO3 adsorbed several dye molecules simultaneously at different temperatures. The results also demonstrated that dye adsorption on avocado peels treated with these acids was exothermic. The estimated adsorption energies for the dye-treated biomass and dye-dye interactions indicated a physisorption associated with van der Waals forces and hydrogen bonding. The outcomes of this study contribute with insights into the adsorption mechanisms of dye molecules using treated avocado peels with the aim of developing sustainable water treatment methods according with a circular economy approach.

Diuretic Activity of Water Fraction of Avocado (Persea Americana Mill.) Leaves Extract in Rat

Currently available diuretics are not completely free from side effects such as electrolyte imbalance and metabolic alterations. Avocado (Persea americana Mill.) contained many type of bioactive substances that related to diuretics. This study aimed to reveal and verify the effect of water fraction of avocado leaves extract against output of rat urine. Three different doses of leaf extract of avocado prepared i.e., 91.125mg/kg BW, 182.25 mg/kg BW and 364.5 mg/kg BW. The urinary parameters assessed were the urine volume hourly for 6 hours, urine color density and urine pH. The results showed water fraction of avocado at the dose of 182.25 and 364.5 mg/kg significantly increase urine volume in comparison to the negative control. Urine color density scores varied but did not have a dependent manner that follows the dose levels of diuretics given. However all treatments showed no statistical difference in the urine pH. Thus, it can be conclude that the water fraction of avocado leaves extract potentially for diuretic herb without make significant changes on pH.

Effectiveness of Sodium Alginate and Carnauba Wax Nanoemulsions with Lemongrass Essential Oil on the quality of ‘Hass’ Avocado Fruit from early, middle, and late harvest season during prolonged cold storage

Avocado (Persea Americana Mill.) has gained popularity as a widely produced and consumed fruit worldwide, raising concerns about its storage and transportation. The avocado, being a climacteric fruit sensitive to chilling injury, faces challenges that affect its shelf life and commercial viability. This research delves into the effectiveness of edible sodium alginate 2 % (weight/weight, w/w) (SA) and carnauba wax 1 % (w/w) (CW) coatings, both independently and in combination with lemongrass essential oil, sodium alginate 2 % (w/w) + lemongrass essential oil 1.25 % (SALG) and carnauba wax 1 % + lemongrass essential oil 1.25 % (w/w) CWLG) as postharvest treatments. Uncoated avocados were used as control (CT). The nanocoating-treatments aim to preserve the quality of 'Hass' avocados harvested during the early, middle, and late season. After treating the fruits, some from each treatment group were kept at 21 ± 1 ºC for 7 days (shelf life). The rest were stored at 5 ºC and 90 % RH for 15, 30, and 45 days before being submitted also to shelf life. In each sampling date, quality parameters measured included: firmness, color (L*, hue), weight loss, gray pulp symptoms, ethylene production and pulp electrolyte leakage. Also, fatty acids (FA), hydrogen peroxide (H2O2), malondialdehyde (MDA), α-farnesene (Farn) and conjugated trienols (Ctrols) were quantified in avocado pulp. Fruit quality analysis showed that all coatings improve quality and reduction of gray pulp development, especially CW and the incorporation of lemongrass in both matrices. Therefore, these edible coatings can be an alternative to improve preservation of avocado fruit quality through cold storage and subsequent shelf-life, which potentially reduce fruit waste.

Potensi Buah Alpukat (Persea americana Mill.) sebagai Media Alternatif Pertumbuhan Jamur Fusarium sp.

Potensi Buah Alpukat (Persea americana Mill.) sebagai Media Alternatif Pertumbuhan Jamur Fusarium sp.

Identification of Phytophthora cinnamomi CRN effectors and their roles in manipulating cell death during Persea americana infection

The oomycete Phytophthora cinnamomi is a devastating plant pathogen with a notably broad host range. It is the causal agent of Phytophthora root rot (PRR), arguably the most economically important yield-limiting disease in Persea americana (avocado). Despite this, our understanding of the mechanisms P. cinnamomi employs to infect and successfully colonize avocado remains limited, particularly regarding the pathogen’s ability to maintain its biotrophic and necrotrophic lifestyles during infection. The pathogen utilises a large repertoire of effector proteins which function in facilitating and establishing disease in susceptible host plants. Crinkling and necrosis effectors (CRN/Crinklers) are suspected to manipulate cell death to aid in maintenance of the pathogens biotrophic and necrotrophic lifestyles during different stages of infection. The current study identified 25 P. cinnamomi CRN effectors from the GKB4 genome using an HMM profile and assigned putative function to them as either cell death inducers or suppressors. Function was assigned to 10 PcinCRNs by analysing their RNA-seq expression profiles, relatedness to other functionally characterised Phytophthora CRNs and tertiary protein predictions. The full-length coding sequences for these PcinCRNs were confirmed by Sanger sequencing, six of which were found to have two divergent alleles. The presence of alleles indicates that the proteins encoded may perform contradicting functions in cell death manipulation, or function in different host plant species. Overall, this study provides a foundation for future research on P. cinnamomi infection and cell death manipulation mechanisms.

Profitability of coffee-based agroforestry system to support farmers’ income in Toba Regency, North Sumatra Province, Indonesia

Indonesia is a country with the application of agroforestry systems on community land. People who live in Toba Regency, North Sumatra have long applied coffee-based agroforestry systems and have become a hereditary tradition. The purpose of this study is to determine the profitability of coffee-based agroforestry land and economic value for farmers so that it can be developed further. Coordinate points of land distribution, taken with GPS, conducted interviews, profitability analysis and income analysis to obtain farmer land data. Mapping the distribution of farmers’ coffee-based agroforestry land was mapped with ArcGIS 10.8. Four respondent farmers implemented coffee-based agroforestry (Coffea arabica) with a planting distance of 2 m x 2.5 m combined with Gmelina arborea (white teak), Eucalyptus sp. (eucalyptus), Cinnamomum verum (cinnamon), Pinus merkusii (pine), Psidium guajava (guava), Durio zibethinus (durian), Persea americana (avocado), and others. Based on the profitability analysis, all four farmers’ lands are feasible for further development. The economic value of land depends on the area of land managed by the farmer, where farmers with a land area of 2 Ha have the largest income during 10 years of land management, namely Mr. Bisken with an income of IDR 162,719,500. Utilization of Multi-Purpose Trees Species (MPTS) or plantation and industrial crops can increase the economic value and profitability of farmers’ land.

The use of the Cartographer (Green Atlas) for determining physiological changes in avocado trees and consequent timing of orchard management toward fruit robustness

The use of the Cartographer (Green Atlas) for determining physiological changes in avocado trees and consequent timing of orchard management toward fruit robustness

Avocado (Persea americana Mill.) Leaf Extract for Diabetic Ulcer Treatment In Vivo

Avocado (Persea americana Mill.) Leaf Extract for Diabetic Ulcer Treatment In Vivo

Nutrient content in avocado fruits: Effect of climate, water management, and flowering type

Avocado is a fruit (Persea americana), that has increased its consumption worldwide. Mexico is the main producer and exporter of avocado in the world, representing more than 30% of the world harvest, of which 73.2% of the total avocado production in Mexico was concentrated in the state of Michoacán. In the state of Michoacán, there is a constant production of avocado fruit throughout the year, but this production is affected by the variation in climate, water management and flowering type, putting agricultural production and sustainable development at risk. The objective of this study was to evaluate the climatic effect, water management, and flowering type on fruit nutrient content in six orchards in the state of Michoacan. Six orchards were evaluated in the towns of Basilia, Cheranguerán, Tecario, Araparicuaro, Peribán and Patamburu in the state of Michoacan, which represent 90% of the climate types (TC), predominant in the state (ACW1, ACW2 and CW1)., with 100% of the water regime (irrigated and seasonal, RH) and 97% of the type of fruit produced (loca and normal, FF). The dry matter contents and total mineral content of the fruit were evaluated. The arrangement of treatments was a factorial [A= water regime (RH); B= type of climate (TC); C= floral flow (FF)], distributed completely randomly with 20 repetitions taking each tree as a repetition. The results indicated that the dry matter content is affected by TC, but not by RH and FF, FF affects the nutritional content more than TC and RH. The type of climate is the factor with the least impact on the mineral content of the fruit. In floral flow, the total nutritional content was significant in four elements with higher values for N, Cl, and Zn in crazy fruit and Na in normal fruit.

Bioaccessibility of bioactive compounds present in Persea americana Mill. seed ingredient during oral-gastric digestion with antibacterial capacity against Helicobacter pylori

Ethnopharmacological relevanceIn ancient Mexican cultures, the Persea americana Mill seed has been used against gastrointestinal diseases, due to high concentrations of bioactive compounds. According to Traditional Mexican Medicine, P. americana seed aqueous infusion is used against roundworms, intestinal worms, parasites, and gastrointestinal problems, in a dose taken over three or four days. In addition, Mexican Society of Natural History indicates the traditional use of P. americana seed powder as an antiparasitic, and antibacterial. On the other hand, Helicobacter pylori infection is a factor associated with the development of gastric disease, peptic ulcers as well as some types of gastric lymphomas and gastric cancer in humans; in this way is necessary scientific evidence about P. americana seed effect in gastrointestinal disease. Aim of the studyThe work aimed to evaluate bioactive compounds bioaccessibility and antimicrobial potential against Helicobacter pylori during oral-gastric digestion in vitro of food ingredient from Persea americana Mill. seed and elucidate the possible action mechanism using in silico tools. Materials and methodsInitially, P. americana seed oil and aqueous extract of P. americana seed were obtained using ultrasound and maceration respectively, and the food ingredient from P. americana seed was obtained. The samples underwent oral-gastric digestions by the INFOGEST method, to continue identifying and quantifying the bioactive compounds by HPLC-DAD and GC-MS. The anti-Helicobacter pylori activity determination were used fourteen Helicobacter pylori clinical strains and reference strains by Susceptibility testing by Minimal Inhibition Concentration, Kinetics of Growth Inhibition of H. pylori, Urease Inhibitory Kinetic. Finally, to elucidate a possible action mechanism used in silico tools (Software AutoDock 4.2.6 and BioVia Discovery v.19.1.0.1.18287). ResultsThe lipophilic fraction of P. americana seed detected oleic acid, linoleic acid, and avocadenofuran compounds, and the phenolic fraction showed the presence of catechin, rutin, ellagic, and chlorogenic acid, among others. Phenolic compounds conformational changes during oral-gastric digestion due to mechanical and acid hydrolysis, while lipophilic compounds showed a 20% increase in the gastric phase. Persea americana Mill. seed ingredient (3.08 μg/mL) showed total in vitro inhibition of clinical and reference strains of H. pylori, likewise, the lipophilic fraction had a lower inhibition concentration (2.59 μg/mL) regardless of the strains. Among the mechanisms found in silico, inhibition of target proteins such as CagA, BabA, and MUC5 were observed, as virulence factors involving adherence and bacterial pathogenicity. ConclusionsThis research provides evidence that food ingredient from P. americana seed has antimicrobial in vitro potential against H. pylori clinical strains, through phenolic and mainly lipophilic compounds, opening new scientific evidence that supports the P. americana seed's traditional use.

Ethanolic extract of Persea americana Mill. (Lauraceae) seeds induced antiestrogenic effects in young female Wistar rats.

The ethanol extract of Persea americana seeds was found to inhibit the development of estrogen-dependent conditions in female Wistar rats, suggesting the ability of its secondary metabolites to interact with estrogen receptors (ERs), either as partial agonists or as antagonists. To test this hypothesis, the abovementioned extract was assessed for its ability to mimic and/or antagonize estradiol effects. Two experiments were conducted in ovariectomized (OVX) rats: (1) animals were treated with estradiol valerate (E2V; 1 mg/kg) or P.americana at doses of 25 and 50 mg/kg; (2) animals were treated with E2V alone (0.75 mg/kg) or in combination with P.americana at the abovementioned doses. Treatments were given orally for 3days and animals were sacrificed for biochemical and histological analyses of the uterus and vagina. When administered alone, P.americana did not change the histomorphology of both organs (uterus and vagina). In combination with E2V, P.americana decreased uterine weight [30 % decrease (p<0.001) at 25 mg/kg and 24 % (p<0.01) at 50 mg/kg] and epithelium height (37 % decrease). This was associated with decreased estradiol levels (at least 86 % decrease, p<0.001) in the uterus. Similarly, vagina epithelium height decreased by at least 34 % (p<0.05) when E2V was co-administered with P.americana. The seed extract of P.americana contains ER antagonist secondary metabolites accounting for its ability to inhibit the development of estrogen-dependent conditions in female rats.

Analysis of the electrophoretic profiles of the protein extracts of the species Manihot esculenta (Crantz, 1975), Persea Americana (Mill., 1768) and Actinidia delicious (A. Chevalier., 1984), and their association with the latex-fruit syndrome for searching for potential allergens

Determine the electrophoretic profiles of the extracts of Manihot esculenta, Actinidia Deliciosa and Persea Americana and their possible relationship with Latex-Fruit Syndrome. Protein extracts of M. esculenta, P. Americana and A. Deliciosa were prepared through the processes of maceration and solvent extraction from plant samples. In the case of the avocado, a prior extraction by soxhlet was carried out to eliminate the fat. The extracts were vacuum filtered, dialyzed and finally lyophilized. Separation of proteins based on molecular weight was performed by SDS PAGE electrophoresis. The electrophoretic profiles obtained were compared with the allergenic proteins previously identified in the latex extract, in order to determine a possible relationship with Latex-Fruit Syndrome, depending on the molecular weight. The extracts of M. esculenta and P. Americana showed a wide range of protein fractions with molecular weights varying from 10 to 250 KD, finding that the region with the highest concentration of bands was between 20 and 89 KD, (60 and 65%), respectively. A 20-band profile was obtained for the M. esculenta extract (Figure 1), with seven bands sharing similar weights with the latex allergens (Hev b 1, Hev b 2, Hev b3, Hev b 4, Hev b 5, Hev b 6.03, Hev b 8 and Hev b 10) (3-5). For the P. Americana extract, 20 bands were also observed (Figure 2), seven of which presented approximate weights to the Latex allergens (Hev b 1, Hev b 2 Hev b 4 Hev b 6.01 Hev b 6.03 Hev b 8 , Hev b 10 Hev b 11 Hev b 14). The Kiwi extract showed two bands of 19.1 and 22.9 KD, with weights close to latex proteins (figure 3), (Hev b 3 and Hev b 6.01), and allergens (Act d 2 and Act d 6), reported in the literature for this fruit. When analyzing the relationship between the separated protein fractions and the latex allergens described in the literature, a possible association of 35% was found for the extracts of M. esculenta and P. Americana, and 10% for A. Delicious, with great relevance being the association found with the allergens Hev b 4, Hev b 2, Hev 8 and Hev b 11, which are involved in Latex-Fruit Syndrome. The electrophoretic profiles of the prepared extracts were determined and compared with the Latex allergens. This information generates a contribution for the development of new research and advances in the standardization of these extracts on a large scale and for their future use in diagnostic tests.

Ajuste osmótico y rendimiento de dos variedades de palta (Persea americana), Hass y Fuerte, con sistema de riego por goteo, en zona andina del Perú

The cultivation of avocado (Persea Americana) is not only essential from an economic point of view, but also plays a crucial role in the nutrition, health and well-being of populations around the world. The study was carried out in Ayacucho -Peru (latitude: 12° 53' 57'' S, longitude: 74° 16' 55'' W and altitude: 2596 masl), with the objective of evaluating the effect of the use of the technical drip irrigation system on the production and yield of two varieties of avocado, Hass and Fuerte. The study was based on the use of an irrigation system with programming by treatment with avocado plants installed in plots of 500 m² in 6 sectors, with a density of 5 x 5 m, whose age was between 5 to 16 years, of the which were considered 12 plants (6 plants with drip irrigation and 6 plants as a control with gravity irrigation). Irrigation was carried out right at the flowering and production stage, guaranteeing adequate water supply in periods of water deficit, between February and June 2022. The experimental design was a complete randomized block, with six blocks of 20 plants each. The harvest carried out reported yields of 67.96 ± 8.8 t/ha under technical irrigation and 26.42 ± 4.93 t/ha under gravity irrigation, an increase of 157% with respect to the latter. As for the varieties, the Fuerte avocado had the highest yield 49.38 ± 23.06 t/ha compared to the Hass variety (45.0 ± 21.14 t/ha), the maximum yield occurred with drip irrigation for the Fuerte variety (71.09 ± 8.38 t/ha), this is explained by the energy expenditure in the osmotic adjustment potential which drastically decreases the yield potential with traditional gravity irrigation, obtaining a minimum yield in Hass of 25.18 ± 4.44 t/ha.

A telomere-to-telomere gap-free reference genome assembly of avocado provides useful resources for identifying genes related to fatty acid biosynthesis and disease resistance.

Avocado (Persea americana Mill.) is an economically valuable plant because of the high fatty acid content and unique flavor of its fruits. Its fatty acid content, especially the relatively high unsaturated fatty acid content, provides significant health benefits. We herein present a telomere-to-telomere gapless genome assembly (841.6Mb) of West Indian avocado. The genome contains 40 629 predicted protein-coding genes. Repeat sequences account for 57.9% of the genome. Notably, all telomeres, centromeres, and a nucleolar organizing region are included in this genome. Fragments from these three regions were observed via fluorescence in situ hybridization. We identified 376 potential disease resistance-related nucleotide-binding leucine-rich repeat genes. These genes, which are typically clustered on chromosomes, may be derived from gene duplication events. Five NLR genes (Pa11g0262, Pa02g4855, Pa07g3139, Pa07g0383, and Pa02g3196) were highly expressed in leaves, stems, and fruits, indicating they may be involved in avocado disease responses in multiple tissues. We also identified 128 genes associated with fatty acid biosynthesis and analyzed their expression patterns in leaves, stems, and fruits. Pa02g0113, which encodes one of 11 stearoyl-acyl carrier protein desaturases mediating C18 unsaturated fatty acid synthesis, was more highly expressed in the leaves than in the stems and fruits. These findings provide valuable insights that enhance our understanding of fatty acid biosynthesis in avocado.

Nutrimental, physicochemical and sensory properties of creole avocado fruits from Parras de la Fuente, Coahuila

The city of Parras de la Fuente, Coahuila is characterized by having criollo avocado trees with different morphological properties. However, very little information is available on their fruit nutritional and sensory characteristics. The objective of this work was to perform the bromatological, mineral, phytochemical and sensory characterization of 7 avocado landraces. The proximal analysis was carried out using standardized tests by the AOAC (2019), while the phytochemical content was determined using HPLC/MS equipment. Subsequently, the samples were evaluated using a hedonic test. The bromatological analysis showed significant differences in the total of the samples for the different response variables, which indicates the great genetic diversity among the sampled trees. The mineral that were determined in the highest concentration were Potassium, Magnesium and Calcium. Thirty-eight different phytochemical compounds were identified in the fruits, with a greater presence of flavones. While the sensory analysis showed a significant difference among the avocado landraces for the evaluated attributes. The flavor and texture attributes were the most important for the consumer preference. Different environmental factors (soil, climate, altitude, latitude, maturity) modify the nutritional composition of the avocado and its organoleptic characteristics. The results of this work may help propagation, fruit diversification and development of companies focused on this fruit in Parras de la Fuente, Coahuila.

TEST OF RESISTANCE OF ALPUKAT (Persea americana mill) FRUIT EXTRACT ON THE GROWTH OF THE Streptococcus mutans

Introduction: Streptococcus mutans is one of the normal flora living in the oral cavity, but in excessive amounts is the main causative agent of dental caries. The active compounds of alkaloids, saponins and quinones in avocado pulp extract is an antibacterial alternative that can inhibit the growth of Streptococcus mutans in the oral cavity. The aim of this research was to determine the inhibitory power that contain in avocado pulp against Streptococcus mutans . Materials and Methods: This study used 4 concentrations namely 25%, 50%, 75% and 100% with each repetition 4 times. Avocado pulp extraction was carried out by maceration method using 96% ethanol solvent. Inhibition zone testing was carried out using the Kirby baurer method. Results and Discussions: The zone of inhibition was determined using the Kruskal wallis test, which showed that there were significant differences in various concentrations against the growth of Streptococcus mutans bacteria. Avocado fruit pulp extract is able to inhibit the growth of Streptococcus mutans bacteria because avocado fruit pulp extract has active compounds that cause antibacterial activity. Active compounds of avocado fruit flesh that are efficacious as antibacterials are alkaloids, saponins and quinones. Conclusion: Avocado pulp extract has been proven to have an antibacterial effect against Streptococcus mutans with an effective concentration level of 100%. Increasing the concentration of avocado pulp extract was also shown to affect the diameter of Streptococcus mutans inhibition zone