Persea Americana Mill Research Articles

SPATIAL ANALYSIS OF ANTHRACNOSE IN AVOCADO (Persea americana Mill.) IN DONATO GUERRA, MEXICO

Anthracnose is a fungal disease caused by Colletotrichum spp. that affects the avocado (Persea americana Mill.) crop and causes significant economic losses in the farming sector. To focus control measures, it is important to understand the spatial distribution and the dynamics followed by the disease under field conditions. The use of methods derived from spatial statistics facilitates this task. The aim of this study was to determine the spatial behavior of anthracnose in Persea americana Mill. cv. Hass in Donato Guerra, a municipality in the State of Mexico, Mexico, using a geostatistical and spatial analysis by distance indices. Four hundred trees were selected and georeferenced in the municipal area. In order to measure the incidence, 48 fruits were selected from every tree. Using these data, the experimental semivariogram was estimated, and adjustments were made to models that explain the spatial arrangement. Maps were created using ordinary kriging, and the infection area was estimated. The maps generated show the presence of aggregation centers and a spatial distribution mostly fitting Gaussian and exponential models, with ranges fluctuating between 12 and 56 m, indicating spatial association between data. Likewise, the greatest percentage of infected areas was 98 %, while the lowest was 45 %. Geostatistics enables a precise understanding of the distribution patterns of diseases such as anthracnose in avocado-growing areas of the State of Mexico, which facilitates the implementation of integrated management programs with greater effectiveness.

Differential Efficacy of Storage Temperature and Postharvest Treatment on Shelf Life and Quality of Avocado Fruit (Persea americana Mill.).

Avocado fruit is a rich source of phytonutrients such as vitamins, minerals, carotenoids, carbohydrates, polyphenols and unsaturated fatty acids. However, due to its climacteric nature, fruits are highly susceptible to storage temperature, resulting in poor shelf life and reduced quality. In the present study avocado fruits (Accession CHES-HA-I/I) were stored at different low temperatures (5, 9 and 12°C with 90-95% relative humidity, RH) to identify optimum low temperature for cold storage. In a further experiment, avocado fruits were treated with 1-methylcyclopropene (1-MCP, 500ppb) and chitosan (0.5%) to extend the shelf life with better fruit quality. The results showed that storage temperatures had significant effect on physiological, biochemical and antioxidant activities of fruits. Lower physiological loss in weight (PLW), reduced respiration and ethylene production, and higher carbohydrates, protein and fat content were recorded in fruits stored at 9°C as compared to 12°C. Similarly, maximum antioxidant properties in terms of free radical scavenging activity (FRSA) and ferric reducing ability of plasma (FRAP) was found in avocado fruits stored at 9°C. It was also noticed that chilling injury was developed in fruits stored under 5°C. In addition, exogenous application of 1-MCP significantly reduced respiration and ethylene production rate at 9°C and extended the shelf life up to 42days with better fruit quality and more antioxidant activities. However, chitosan treated and control fruits had shelf life up to 28 and 21days respectively, with minimum nutritional content. From this study it is concluded that a storage temperature of 9°C and 1-MCP treatment significantly enhanced the shelf life of avocado fruits with better fruit quality as compared to other storage temperatures (5 and 12°C) and postharvest treatment (chitosan).

Teaching methods to grow (persea americana mill) obtained from seed in a family yard

Teaching methods to grow (persea americana mill) obtained from seed in a family yard

Study of the physicochemical properties of hass avocado oil encapsulated by complex coacervation

This study aimed to encapsulate Hass avocado (Persea americana Mill. cv. Hass) oil through complex coacervation using a gelatin:sodium alginate (NaAlg) mixture and assess the physicochemical properties of the resulting encapsulates. Turbidity, z-potential, and efficiency analyses indicated that the optimal conditions for oil encapsulation were a pH value of 4.0 and a gelatin:NaAlg solids ratio of 3:1. Coacervates formed with lower concentrations of solids exhibited superior morphologies compared to those obtained with higher concentrations. Encapsulation yield ranged from 34.7 to 86.8%, and the encapsulation efficiency ranged between 57.4 and 92.2%. Furthermore, the samples demonstrated low water activity (ranging from 0.01 to 0.36) and moisture content (2.18–9.25%), showcasing the high microbiological stability of the powder. Overall, the encapsulates displayed low hygroscopicity (<15%), suggesting enhanced technological stability for the product over time. Additionally, they exhibited good thermal stability, with degradation initiation temperatures surpassing 190 °C. This study highlights the technological potential of complex coacervation as a method to encapsulate Hass avocado oil. This approach offers a promising and viable alternative to use this material in various industrial applications.

Comparison of Antioxidant Activity of Methanol Extract of Young and Old Leaves of Avocado (Persea americana Mill.)

Avocado plants (Persea americana Mill.) are widely grown in tropical and subtropical regions. Avocado leaves contain natural antioxidants for traditional medicine. This study aims to determine the total phenolic content, total flavonoids, and antioxidant activity values of young and old avocado leaves, and analyze the relationship between total phenol content and total flavonoids to the antioxidant activity value of avocado leaf methanol extract (Persea americana Mill.). The macerated avocado leaf extract was quantitatively tested for antioxidant activity using the DPPH method and the total phenolic and flavonoid contents were calculated using the Follin-Ciocalteu and Follin-Ciocalteu methods, respectively.The results showed that the highest total phenolic content of old avocado leaves was 1.682,27mg GAE/100g dry weight. The highest total flavonoid content of avocado old leaves was 3.858,96mg QE/100g dry weight. Antioxidant activity of methanol extract of young avocado leaves has a value of IC50 321.32 ± 80.43 ppm and avocado old leaves amounted to 288.54 ± 33.06 ppm. Total flavonoid content was more influential on antioxidant activity, with a correlation coefficient of 0.856

Effect of blanching conditions and solar drying on selected nutritional, anti-nutritional and bioactive components of formulated avocado seed powder

Avocado (Persea americana Mill) seeds are mainly considered as waste by-products of avocado fruit processing in many countries, including Tanzania despite their potential health and economic benefits. The present work investigated the effect of hot water blanching and solar drying treatments on nutritional, bioactive, and cyanogenic glycosides of avocado seed samples in an attempt to develop quality avocado seed powders (ASPs) by using solar drying technology. Results showed that total ash, crude protein, crude fiber, total phenolic, proanthocyanidins, and cyanogenic glycosides contents showed a significant reduction in avocado seed powders with respect to increased blanching temperature and time. On the other hand, crude fat and total carotenoids contents, showed a decline with respect to increased temperature only. Blanching treatment at 85 °C for 10 minutes retained a significant amount of total ash (86.9%), crude fat (100%), crude protein (78.8%), crude fiber (91.6%), total carotenoids (43.6%) and total phenol contents (47.0%) in developed avocado seed powder. Cyanogenic glycosides were highly reduced at 95 °C for 20 minutes (81.1%) than at 85 °C for 10 minutes (58.5%). However, the level of cyanogenic glycosides in all avocado seed powders are far below the lethal dose (36 mg/100 g) of hydrocyanic acid. The significant high losses of nutrients and phytochemicals was observed at a blanching treatment of 95 °C for 20 minutes. This could be due to prolonged leaching of soluble compounds to hot water and degradation of heat-sensitive bioactive compounds. The aforementioned results revealed that, different temperatures and times during hot water blanching significantly influence the quality of formulated avocado seed powder. Therefore, hot water blanching treatment at 85 °C for 10 minutes and solar drying technology could be employed to produce a nutrient-rich and palatable avocado seed powder that can find its use in normal food consumption and food processing industries, particularly in low income settings. Key words: Avocado seed, avocado seed powder, solar drying, hot water blanching, utilization

Modelling pollination maps in agroecosystems of a Chilean biodiversity hotspot

ABSTRACT Wild pollinators play a crucial role in maintaining healthy ecosystems and sustaining agricultural productivity. However, their survival is at risk due to habitat loss and land use changes, exacerbating the ongoing biodiversity crisis, and jeopardising global food security. We assessed the contribution of native vegetation cover to pollination and avocado crop yield by modelling the abundance of native bees and the pollination services they provide in agroecosystems of the Aconcagua River basin in Central Chile. This region stands as a critical biodiversity hotspot, currently confronted with the challenge of land use change, especially due to the expansion of avocado (Persea americana Mill.) monocultures. Our findings revealed a clear relationship between native bee abundance, pollination services, and avocado yield as a function of natural vegetation cover surrounding the crops. Areas with a higher proportion of native vegetation exhibited the most abundant supply of pollination services, while within avocado crops show lower levels of pollination support. A spatial mismatch between supply and demand for pollination revealed by our results indicates that agricultural expansion threatens native pollinators and agricultural yield production in this region. The development of pollination maps has been proposed as a strategic tool to guide land-use planning decisions within agricultural landscapes. Prioritizing the conservation of native vegetation or adopting biodiversity-friendly practices can help mitigate the effects of agricultural expansion on wild pollinators. These strategies are vital for safeguarding biodiversity, ensuring food security, and mitigating the far-reaching impacts of the current biodiversity crisis.

Nutritional and Anti-oxidative Potentials of Ethanolic Extract of Avocado Pulp (Persea americana Mill) On Caffeine Induced Oxidative Stress on Wistar Rats

Nutritional and Anti-oxidative Potentials of Ethanolic Extract of Avocado Pulp (Persea americana Mill) On Caffeine Induced Oxidative Stress on Wistar Rats

Ecostructure and Endemicity of Plant Species in Lowland Plantation Typology (Hortipark) Karang Sidemen Village, West Nusa Tenggara

The richness of local resources and the ecosystem potential of a village, one of which is the uniqueness of the lowland plantation ecosystem (hortipark), is the main key in supporting the success of sustainable rural development. The main focus of the research is to reveal the ecostructure and endemicity of plant biodiversity in the lowland plantation typology of Karang Sidemen Village, NTB. Data collection in the field used a systematic sampling design with random start method for 12 research plots. The results of observations found 1116 individuals, 29 tribes, 59 genera and 59 plant species at all levels of habitus, and there were 20 plant species endemic to Lesser Sunda. The highest INP tree is Ceiba pentandra (L.) Gaertn. (111.79%), stake rate of Tectona grandis Lf (136.97%), stake rate of Persea americana Mill. (82.86%), Dendrocnide stimulant (Lf) Chew seedling rate (130.48%), Musa × paradisiaca L. shrub rate (81.28%), Panicum brevifolium L. herb rate (32.53%), Chloranthus erectus (Buch-Ham) shrub rate) Wall. (46.64%), the level of the fern Macrothelypteris torresiana (71.22%) and the level of the liana Centrosema pubescens Benth (96.99%). The concentration of horizontal structures is in diameter classes 2 and 4 while the concentration of vertical structures is in class 1. The H' Index range has values ​​in the low - medium category with the highest H' being at the livestock level of 2.64. The E' index is in the medium - high category with the highest E' at the shrub level of 1.00. The R1 index is in the low - medium range, with the highest R1 at the livestock level of 3.45. The distribution of plant species is mostly clustered, especially at the herb level. Based on the Raunkiaer frequency class law, it is known that species in the lowland hortipark plantation ecosystem of Karang Sidemen Village are included in the type of artificial ecosystem with the highest frequency in class A (1-20%).

Antibacterial activity of avocado extract (Persea americana Mill.) against aminoglycoside-resistant Klebsiella pneumoniae strains

Klebsiella pneumoniae is an important etiological agent of nosocomial infections. This study focuses on the antibacterial activity of Persea Americana extract against K. pneumoniae strains containing aminoglycoside genes. Broth microdilution technique was used to examine the antibacterial activity of P. americana extract on K. pneumoniae isolates. The phenolic components of its leaves were analysed by the HPLC-DAD system. The binding potential of the major phytochemical component of the extract, chlorogenic acid, to the KsgA was investigated by molecular docking. The stability of the resulting enzyme-ligand complexes was also assessed through molecular dynamics simulation. The result obtained showed that P. americana extract had antibacterial activity on aminoglycoside resistant strains. The binding potential of chlorogenic acid (main phenolic compound) to KsgA was found to be higher than the binding of gentamicin. Since chlorogenic acid had lower binding energy and formed higher number of H-bonding as well as other interactions in the docking analysis. The higher binding potential of the major component with the enzyme that was involved in resistance development against aminoglycoside indicated its possible role in overturning the condition. Moreover, the molecular dynamics simulation study revealed that chlorogenic acid formed a complex with relatively higher stability. The higher binding potential of the major component with higher stability implied that the computational results confirmed the experimental findings.

Ovicidal Effect of the Ethanolic Extract of Moringa oleifera Leaf on Oligonychus punicae (Trombidiformes: Tetranychidae) Eggs1

Abstract Mexico is the leader in avocado (Persea americana Miller [Lauraceae]) production, with the United States being the principal consumer of avocados produced there. The avocado crop is susceptible to attacks by insect and mite pests, including the avocado brown mite, Oligonychus punicae (Hirst) (Acari: Tetranychidae), which is a serious economic pest of the crop in Mexico. This study evaluated the effects of different concentrations (0.1, 0.5, 1, 5, 10, 15, and 20% [v/v]) of the ethanolic extract from Moringa oleifera Lamarck (Moringaceae) leaves against O. punicae eggs and its residual effect on the density of larvae and nymphs emerging from treated eggs. Egg mortality ranged from 0.0 at the 0.1% concentration to 70.48% at the 20% concentration, compared with the control group. Egg mortality increased as extract concentrations increased. The density of larvae and nymphs that emerged from the treated eggs was significantly lower than the density in the control, indicating that both the number of living larvae and nymphs responded to extract concentration. Our results support the suggestion that the ethanolic extract of M. oleifera leaf has ovicidal and residual activity against O. punicae and can be incorporated into an integrated mite management program to control avocado brown mite populations. Further studies are needed to assess the efficiency and efficacy of M. oleifera extract against arthropod pests and natural enemies under greenhouse and field conditions.

In Silico Study of Avocado Leaves (Persea americana Mill.) as Inhibitor of Calcium Oxalate Urolithiasis

In Silico Study of Avocado Leaves (Persea americana Mill.) as Inhibitor of Calcium Oxalate Urolithiasis

Effect of shade tree on nutrient concentration in coffee (Coffea arabica L.) leaves

Abstract Leaf tissue analysis is a measurement of the levels of elements contained in plant leaves. In this case, the leaves can describe the amount of absorption of a nutrient because most of the assimilation results are collected in them. The nutrients that can be absorbed by plant roots are the amount of nutrients available in the soil. The use of shade trees with different types of sunlight absorption intensity causes the conditions for leaf nutrient absorption in plants to also vary. This research was needed to determine the effect of shade type on the nutrient concentration of nitrogen, (N), phosphorus (P), and potassium (K) in the leaves of Arabica coffee plants. This research was carried out at the Koperasi Ribang Gayo Musara, Pantan Musara Village, Pegasing Subdistrict, Central Aceh District, Aceh, and the Agricultural Instrument Standardization Agency (BSIP) Aceh. This research used a non-factorial Randomized Block Design with five treatments where each treatment 4 replications. Treatments consisted of types of shade plants on coffee plants, namely, control/no shade, small lamtoro shade (Leucaena leucocephala ssp. leucocephala), big lamtoro shade (Leucaena leucocephala subsp. glabrata), banana shade (Musa paradisiaca L.), avocado shade (Persea americana Mill.). The results showed that shade tree type significantly affect potassium (K) nutrient levels in coffee plant leaves. Coffee plant shaded by big lamtoro had better N, P and K nutrient levels compared to small lamtoro, banana, avocado, and control.

Antibacterial Potential of Ethanolic Extract of Avocado Leaves (Persea americana mill.) against Clinical Isolate of Klebsiella pneumoniae and Proteus mirabilis

Multidrug resistance is a global health problem that is closely related to microbial resistance. Klebsiella pneumonia and Proteus mirabilis are opportunistic bacteria that cause urinary tract infections, especially in the use of catheters in hospitals in inpatient care. Handling it using commercial antibiotics can increase the effect of antibacterial resistance if the use is irrational and neglected. Avocado leaves have been reported to have antibacterial, antidiabetic and antioxidant activity. The purpose of this study was to observe into the antibacterial activity of avocado leaves ethanol extract against K. pneumoniae and P. mirabilis. The minimum inhibitory concentration (MIC) was determined using the Kirby-Bauer method, the minimum bactericidal concentration (MBC) was determined using the streaking method of the inhibition zone formed, and the mechanism of bacterial membrane leakage cell was measured using UV-VIS spectrophotometry at wavelengths of 260 and 280 nm. The MIC value was found to be 3.125 mg/mL in both bacteria, with an inhibition zone of 6.43 ± 0.06 mm (K. pneumoniae) and 6.73 ± 0.15 mm (P. mirabilis). The MBC value at 200 mg/mL with a percent reduction of 98.35 % (K. pneumoniae) and at 100 mg/mL with a percent reduction of 98.06 % (P. mirabilis). The supernatant of a membrane leakage cell (DNA and protein) was absorbed at wavelengths of 260 and 280 nm. It can be concluded that the ethanolic extract of avocado leaves has antibacterial potential against clinical isolates of K. pneumoniae and P. mirabilis. HIGHLIGHTS Avocado leaves, which are typically discarded, can be repurposed as a potent antibacterial agent in traditional medicine Demonstrates a potent minimum inhibitory concentration with a minimum bactericidal concentration value that can effectively suppress bacterial growth Point number 2 is substantiated by the utilization of DNA and protein cell leakage tests, as evidenced by absorbance measurements at wavelengths of 260 and 280 nm Flavonoid secondary metabolite have been successfully identified as one of the active components using the TLC analysis method GRAPHICAL ABSTRACT

Diuretic Activity of Water Fraction of Avocado (Persea Americana Mill.) Leaves Extract in Rat

Currently available diuretics are not completely free from side effects such as electrolyte imbalance and metabolic alterations. Avocado (Persea americana Mill.) contained many type of bioactive substances that related to diuretics. This study aimed to reveal and verify the effect of water fraction of avocado leaves extract against output of rat urine. Three different doses of leaf extract of avocado prepared i.e., 91.125mg/kg BW, 182.25 mg/kg BW and 364.5 mg/kg BW. The urinary parameters assessed were the urine volume hourly for 6 hours, urine color density and urine pH. The results showed water fraction of avocado at the dose of 182.25 and 364.5 mg/kg significantly increase urine volume in comparison to the negative control. Urine color density scores varied but did not have a dependent manner that follows the dose levels of diuretics given. However all treatments showed no statistical difference in the urine pH. Thus, it can be conclude that the water fraction of avocado leaves extract potentially for diuretic herb without make significant changes on pH.

Effectiveness of Sodium Alginate and Carnauba Wax Nanoemulsions with Lemongrass Essential Oil on the quality of ‘Hass’ Avocado Fruit from early, middle, and late harvest season during prolonged cold storage

Avocado (Persea Americana Mill.) has gained popularity as a widely produced and consumed fruit worldwide, raising concerns about its storage and transportation. The avocado, being a climacteric fruit sensitive to chilling injury, faces challenges that affect its shelf life and commercial viability. This research delves into the effectiveness of edible sodium alginate 2 % (weight/weight, w/w) (SA) and carnauba wax 1 % (w/w) (CW) coatings, both independently and in combination with lemongrass essential oil, sodium alginate 2 % (w/w) + lemongrass essential oil 1.25 % (SALG) and carnauba wax 1 % + lemongrass essential oil 1.25 % (w/w) CWLG) as postharvest treatments. Uncoated avocados were used as control (CT). The nanocoating-treatments aim to preserve the quality of 'Hass' avocados harvested during the early, middle, and late season. After treating the fruits, some from each treatment group were kept at 21 ± 1 ºC for 7 days (shelf life). The rest were stored at 5 ºC and 90 % RH for 15, 30, and 45 days before being submitted also to shelf life. In each sampling date, quality parameters measured included: firmness, color (L*, hue), weight loss, gray pulp symptoms, ethylene production and pulp electrolyte leakage. Also, fatty acids (FA), hydrogen peroxide (H2O2), malondialdehyde (MDA), α-farnesene (Farn) and conjugated trienols (Ctrols) were quantified in avocado pulp. Fruit quality analysis showed that all coatings improve quality and reduction of gray pulp development, especially CW and the incorporation of lemongrass in both matrices. Therefore, these edible coatings can be an alternative to improve preservation of avocado fruit quality through cold storage and subsequent shelf-life, which potentially reduce fruit waste.

Potensi Buah Alpukat (Persea americana Mill.) sebagai Media Alternatif Pertumbuhan Jamur Fusarium sp.

Potensi Buah Alpukat (Persea americana Mill.) sebagai Media Alternatif Pertumbuhan Jamur Fusarium sp.

Avocado (Persea americana Mill.) Leaf Extract for Diabetic Ulcer Treatment In Vivo

Avocado (Persea americana Mill.) Leaf Extract for Diabetic Ulcer Treatment In Vivo

Bioaccessibility of bioactive compounds present in Persea americana Mill. seed ingredient during oral-gastric digestion with antibacterial capacity against Helicobacter pylori

Ethnopharmacological relevanceIn ancient Mexican cultures, the Persea americana Mill seed has been used against gastrointestinal diseases, due to high concentrations of bioactive compounds. According to Traditional Mexican Medicine, P. americana seed aqueous infusion is used against roundworms, intestinal worms, parasites, and gastrointestinal problems, in a dose taken over three or four days. In addition, Mexican Society of Natural History indicates the traditional use of P. americana seed powder as an antiparasitic, and antibacterial. On the other hand, Helicobacter pylori infection is a factor associated with the development of gastric disease, peptic ulcers as well as some types of gastric lymphomas and gastric cancer in humans; in this way is necessary scientific evidence about P. americana seed effect in gastrointestinal disease. Aim of the studyThe work aimed to evaluate bioactive compounds bioaccessibility and antimicrobial potential against Helicobacter pylori during oral-gastric digestion in vitro of food ingredient from Persea americana Mill. seed and elucidate the possible action mechanism using in silico tools. Materials and methodsInitially, P. americana seed oil and aqueous extract of P. americana seed were obtained using ultrasound and maceration respectively, and the food ingredient from P. americana seed was obtained. The samples underwent oral-gastric digestions by the INFOGEST method, to continue identifying and quantifying the bioactive compounds by HPLC-DAD and GC-MS. The anti-Helicobacter pylori activity determination were used fourteen Helicobacter pylori clinical strains and reference strains by Susceptibility testing by Minimal Inhibition Concentration, Kinetics of Growth Inhibition of H. pylori, Urease Inhibitory Kinetic. Finally, to elucidate a possible action mechanism used in silico tools (Software AutoDock 4.2.6 and BioVia Discovery v.19.1.0.1.18287). ResultsThe lipophilic fraction of P. americana seed detected oleic acid, linoleic acid, and avocadenofuran compounds, and the phenolic fraction showed the presence of catechin, rutin, ellagic, and chlorogenic acid, among others. Phenolic compounds conformational changes during oral-gastric digestion due to mechanical and acid hydrolysis, while lipophilic compounds showed a 20% increase in the gastric phase. Persea americana Mill. seed ingredient (3.08 μg/mL) showed total in vitro inhibition of clinical and reference strains of H. pylori, likewise, the lipophilic fraction had a lower inhibition concentration (2.59 μg/mL) regardless of the strains. Among the mechanisms found in silico, inhibition of target proteins such as CagA, BabA, and MUC5 were observed, as virulence factors involving adherence and bacterial pathogenicity. ConclusionsThis research provides evidence that food ingredient from P. americana seed has antimicrobial in vitro potential against H. pylori clinical strains, through phenolic and mainly lipophilic compounds, opening new scientific evidence that supports the P. americana seed's traditional use.

Ethanolic extract of Persea americana Mill. (Lauraceae) seeds induced antiestrogenic effects in young female Wistar rats.

The ethanol extract of Persea americana seeds was found to inhibit the development of estrogen-dependent conditions in female Wistar rats, suggesting the ability of its secondary metabolites to interact with estrogen receptors (ERs), either as partial agonists or as antagonists. To test this hypothesis, the abovementioned extract was assessed for its ability to mimic and/or antagonize estradiol effects. Two experiments were conducted in ovariectomized (OVX) rats: (1) animals were treated with estradiol valerate (E2V; 1 mg/kg) or P.americana at doses of 25 and 50 mg/kg; (2) animals were treated with E2V alone (0.75 mg/kg) or in combination with P.americana at the abovementioned doses. Treatments were given orally for 3days and animals were sacrificed for biochemical and histological analyses of the uterus and vagina. When administered alone, P.americana did not change the histomorphology of both organs (uterus and vagina). In combination with E2V, P.americana decreased uterine weight [30 % decrease (p<0.001) at 25 mg/kg and 24 % (p<0.01) at 50 mg/kg] and epithelium height (37 % decrease). This was associated with decreased estradiol levels (at least 86 % decrease, p<0.001) in the uterus. Similarly, vagina epithelium height decreased by at least 34 % (p<0.05) when E2V was co-administered with P.americana. The seed extract of P.americana contains ER antagonist secondary metabolites accounting for its ability to inhibit the development of estrogen-dependent conditions in female rats.