Background. The results of revision anterior cruciate ligament reconstruction (ACLR) are usually worse than primary ACLR. The positive outcome of the revision ACLR is significantly influenced by the following factors: anatomical position of bone tunnels, correct of graft choice, preparation, and fixation method, as well as appropriate determination of indications for additional extra articular interventions. Aim of the study was to evaluate the results after a single-stage revision ACLR using a peroneus longus tendon (PLT) autograft and compare them with the results of primary ACLR performed using a standard technique using a hamstring tendons (HT). Methods. The comparison was carried out between the study group – "RACL-PLT" (n=29) – single-stage revision ACLR using an PLT autograft and the control group – "PACL–HT" (n=82) – primary ACLR according to a standard procedure using a HT autograft. Subjective and objective evaluation using the KOOS, IKDC, and Lysholm scales was performed, and the position of the central entry points into the primary and revision tunnels was also determined in the «RACL-PLT» group. Results. There were no statistically significant differences in the results of an objective assessment of knee joint stability between the groups (p0.999). Significantly higher results of subjective assessment on the Lysholm, KOOS and IKDC scales were obtained in the «PACL-HT» group (p 0.001). The position of the central entry points into the revision tunnels corresponded to the normative values in all cases. Conclusion. The analysis of the use PLT autograft in revision ACLR, as well as the achievement of the anatomical position of the revision tunnels, showed that the results of the subjective assessment of the revision and primary ACLR are significantly different, and the results of the objective assessment are comparable.