-Daytime refuge selection, nest construction, and patterns of refuge use of cotton mice (Peromyscus gossypinus) and golden mice (Ochrotomys nuttalli) were studied in S-central Florida. Of 95 P. gossypinus refugia examined, 67 were burrows of the gopher tortoise (Gopherus polyphemus), 23 were ground holes, and five were hollow tree cavities. Of 77 0. nuttalli refugia examined, 75 were on the ground under leaf litter and two were aboveground in shrubs. Neither species differed in seasonal refuge selection. Peromyscus gossypinus built platform and spherical nests of saw palmetto (Serenoa repens) fibers, lichens and cotton, and 0. nuttalli built spherical nests of saw palmetto fibers, Spanish moss, leaves, twigs, grasses and cotton. Winter 0. nuttalli nests were significantly larger and heavier than summer nests. Individuals of both species used a similar number of refugia, and refuge fidelity was greater for both species during winter. The greater versatility of refuge selection in P. gossypinus may contribute to its ability to exploit a wider variety of habitats than 0. nuttalli. Based on the extreme differences in refuge selection observed between these species, it is unlikely that competition for daytime refugia occurred in this study.