ABSTRACTThe dinoflagellate Amphidinium cryophilum sp. nov. is one of the few gymnodinians to be studied at the ultrastructural level. It resembles other dinoflagellates in the structure of the nucleus, trichocysts, storage materials, flagella, mitochondria, and microbodies. Other features of A. cryophilum less commonly observed in related organisms include a network of small interconnected vesicles, a system of large, peripheral vacuoles, chloroplasts bound by two rather than three membranes, an accumulation body, thylakoid‐associated plastoglobuli, a vesiculated nuclear envelope, a complex tubular pusule, striated flagellar collars, collared pits, and a peduncle. The occurrence of a peduncle, a structure implicated in phagotrophy, in this autotrophic organism is noteworthy. The ultrastructure of the peduncle of A. cryophilum differs significantly from that reported in another dinoflagellate.