Event Abstract Back to Event PRRXl1 Homeodomain Transcription Factor Binds to its Own Promoter in Vivo Filipe A. Monteiro1, C. Reguenga1, Francois Guillemot2, D. S. Castro2 and Deolinda Lima1* 1 University of Porto, Laboratory of Molecular Cell Biology, Faculty of Medicine of Porto and Morphophysiology of the somatosensory System, Instituto de Biologia Celular e Molecular, Portugal 2 National Institute for Medical Research, Mill Hill, Division of Molecular Neurobiology, United Kingdom Prrxl1 is a paired-like homeodomain (HD) transcription factor involved in the development/maintenance of both peripheral and spinal cord dorsal horn nociceptive neurons, dedicated to the processing of pain input. Contrary to the broad knowledge on the defects displayed at the cellular level by the absence of Prrxl1, very little is known about the transcriptional network downstream this factor. Dragon, a member of the repulsive guidance molecule-related family that is co-expressed with Prrxl1 in embryonic DRG and spinal cord, is so far the only well established Prrxl1 target gene. Here we show, by the use of in silico analysis, that the transcription factor binding sites on Prrxl1 proximal promoter present a conserved HD putative binding site, suggesting the existence of an auto-regulatory mechanism controlling Prrxl1 expression. In vitro DNA pull- down assays using ND7/23 neuronal cell line overexpressing Prrxl1 show that Prrxl1 interacts with its own promoter. This same interaction was confirmed in vivo by chromatin immunoprecipitation (ChIP) performed on mouse dorsal spinal cord tissue at embryonic age 14.5 (E14.5). Gain-of-function experiments in ND7/23 cells reveal differential autoregulatory expression by two Prrxl1 isoforms. Transcriptional autoregulation of Prrxl1 is in line with its highly restricted expression pattern. Conference: 11th Meeting of the Portuguese Society for Neuroscience, Braga, Portugal, 4 Jun - 6 Jun, 2009. Presentation Type: Poster Presentation Topic: Abstracts Citation: Monteiro FA, Reguenga C, Guillemot F, Castro DS and Lima D (2009). PRRXl1 Homeodomain Transcription Factor Binds to its Own Promoter in Vivo. Front. Neurosci. Conference Abstract: 11th Meeting of the Portuguese Society for Neuroscience. doi: 10.3389/conf.neuro.01.2009.11.133 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 11 Aug 2009; Published Online: 11 Aug 2009. * Correspondence: Deolinda Lima, University of Porto, Laboratory of Molecular Cell Biology, Faculty of Medicine of Porto and Morphophysiology of the somatosensory System, Instituto de Biologia Celular e Molecular, Porto, Portugal, limad@med.up.pt Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Filipe A Monteiro C. Reguenga Francois Guillemot D. S Castro Deolinda Lima Google Filipe A Monteiro C. Reguenga Francois Guillemot D. S Castro Deolinda Lima Google Scholar Filipe A Monteiro C. Reguenga Francois Guillemot D. S Castro Deolinda Lima PubMed Filipe A Monteiro C. Reguenga Francois Guillemot D. S Castro Deolinda Lima Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.