The study was focused on the effectiveness of Centella asiatica extract in microbial reduction and retarding browning formation of the samples obtained from fresh-cut fruits and vegetables (FCFV) during room temperature displayed (RTD) and chilled storage (CS). The microbial and color analyses were done in hour-basis (room temperature displayed) and daily basis (chilled storage). Candida albicans (6.77 log CFU/mL), Bacillus cereus (6.34 log CFU/mL), Escherichia coli O157:H7 (6.46 log CFU/mL) and Aspergillus niger (5.34 log CFU/mL) were specifically inoculated in apple, potato, lettuce, and pineapple, respectively. In room temperature displayed samples, the population of survival microorganisms ranged from 4.44 to 6.90 log CFU/g. In chilled stored samples, only A. niger was survived in chilled pineapple (3.00 log CFU/g) when storage end. Browning indexes in room temperature displayed and chilled stored samples ranged from 8.06 to 9.26 and 5.00 to 12.68, respectively. In short, low temperature and sufficient extract-treated periods may effectively inhibit microbial growth and browning index to prevent quality deterioration of fresh-cut fruits and vegetables.