Harvest Period Research Articles

Soil Enzyme Responses and Crop Productivity Indices Assessment in Agricultural Soil Impacted with Heavy Metals

The present study assessed the productivity of cultivated garden vegetable among other ecological assessments in soil samples impacted with heavy metals. Assay of soil enzyme responses showed the activity of: catalase, peroxidase, lipase and ureasewith corresponding OD reading of 0.750, 2.05, 0.22 and 1.704 respectively. There were noticeable increases in the activity of ureaseandperoxidasewhilecatalaseandlipaseactivitywererelativelylowinthesampledsoil. Out of the 321g of the vegetable seedstested for viability, 45% representing 144.45g was used for the planting and further experiment. After three days of cultivation, germination process was recorded faster in the <i>T. oleifera </i>potted ridge while <i>Amaranthus </i>and <i>Solanum </i>sp seed took 3-4 day for break full dormancy. Determination of total chlorophyll a and b in the selected vegetables showed a correlative increase in chlorophyll a and b in soils contaminated with Zn, Cu and Fe in all the cultivated vegetables: T<i>. oleifera</i>, <i>Amaranthus </i>and <i>Solanum </i>sp, respectively. There are significant increase in total cholorphyl a (0.9mg/g) and b (0.8mg/g) contents from the results when compared with the control experiment as other soil contaminated with heavy metals such as: Pb, and As repressed the selected vegetables cultivated in the soil samples. However total chlorophyll a was seems lightly higher than cholophyl b in all the selected vegetable cultivated in soil. Analysis on the impact of heavy metals on the shootlength of the cultivated vegetables analysed for thirty-one days showed regressive increase in the shootlength of the cultivated vegetables as the period of harvest increases from 0-31 when compared with the control experiment. However Cd and As had the most estimated impact on the vegetables in all the cultivated soils and its impact progresses as the period of harvest increases. Dry matter weight contents of the cultivated vegetables cultivated in the polluted soils were analysed; also the same index was assessed in the vegetables from the unpolluted soils. There was a significant increase in the dry matter contents of the cultivated vegetables in soil polluted with Cu, Fe and Zn respectively. However, dry matter contents were seen progressively low in vegetables cultivated in soils polluted with Cd, As and Pb. When compared with the control experiment. The results from the present study have shown the vulnerability of agricultural soil and cultivated vegetables to effluent from industrial bias sources used for irrigation and its impact on agricultural productivity.

Breeding peculiarities of the manifestation of hemp fiberness signs

Aim. To establish the genetic features of the manifestation of signs of fibrousness and yield of stems, depending on growing conditions and harvesting periods. Methods. New methods were applied: "Method of evaluation of hemp plants for the presence of cannabinoids", "Method of evaluation of individual plants of monoecious hemp varieties by fiber content at the beginning of flowering", "Method of creation of hybrid breeding material of monoecious hemp". Results. It was established that the main conditions for obtaining the maximum amount of fiber and its quality indicators are the use of the late-ripening variety Hlukhivski 51, sowing with a feeding area of 15x5 cm, the diameter of the stems 6-8 mm, the sowing rate of 75 kg/ha (4.2 million pcs./ha) against the background of increased fertilization. Conclusions. The genetic features of the Hlukhivski 51 variety are high fibrousness and high yield of the stems, which are maximally manifested by observing the conditions of the feeding area of 15x5 cm, the sowing rate of 75 kg/ha (4.2 million pieces/ha) and the increased background of fertilization (N120P90K90). Expansion of the response of genotypes of late-ripening varieties to the level of dry biomass yield of 20 t/ha occurs with a feeding area of 50x5 and 50x10 cm and increased doses of N150P120K120 fertilizers. This can become the basis for the use of hemp for bioenergy purposes. When growing production crops for fiber in order to speed up harvesting, an important factor is the start of harvesting at an earlier time, and therefore we recommend growing two varieties of hemp: early-ripening (technical maturity occurs 2 weeks earlier than late-ripening varieties) and late-ripening varieties of hemp.

Effects of soil amendments and coverings on the kenaf yield and soil physicochemical properties in saline-alkali land

In order to investigate the effects of various treatments on the growth of kenaf in saline soil, this study employed a comprehensive experimental design incorporating a dual approach of soil amendment and mulching techniques. Soil treatment of saline and alkaline land, as well as biological material mulching treatment after sowing kenaf, were conducted. The growth dynamics of kenaf were assessed before the mid-term investigation, and the harvest period was examined. Soil physicochemical properties were measured and analysed at different periods. The results show that in the analysis of red sesame yield traits, the differences mainly appeared between soil treatments. There is no significant difference between the different mulching treatments within each soil treatment. In the mid-term study, significant differences were observed in kenaf plant height. Plant height was the most affected by soil conditioners, while other yield traits did not show significant variations. During harvest, only stem thickness and dry weight of a single plant did not show significant differences, whereas other yield traits did. The application of soil conditioners resulted in a 58.15% increase in dry hull yield compared to organic fertilizers and a 22.66% increase compared to regular fertilizers. Soil conditioners also led to higher levels of effective phosphorus, quick-acting potassium, organic matter, and total nitrogen throughout the kenaf growth period compared to organic and regular fertilizers. This is beneficial for enhancing soil quality and reducing soil alkalinity. Combined with the results of kenaf yield and soil physical and chemical properties analysis, it has been proven that the application of soil conditioner has the best effect, followed by the application of compound fertilizer, with organic fertilizer treatment being the least effective.

Enhancing the fruit yield and quality of pomegranate in a new niche area: Insights into site specific agronomic practices

The sustained higher profitability of a pomegranate orchard relies heavily on the production of a greater proportion of high-quality fruits, a goal achievable through the implementation of effective management practices. The objective of this study is to provide site-specific supplementary knowledge regarding the response of pomegranate plants to various management practices under arid conditions. With the aim of enhancing both marketable fruit yield and quality of pomegranate in arid regions, four separate and simultaneous experiments were conducted in the same orchard to evaluate (i) the response of planting materials derived from tissue culture, air layering, and cutting; (ii) the impact of crop (fruit) load; (iii) the response of crop regulation; and (iv) the application response of organo-mineral fertilizers containing potassium (OMF-K) and phosphorus (OMF-P). The results obtained for plant growth, fruit yield, and fruit quality did not exhibit significant differences among pomegranate plants raised through the three different methods of vegetative propagation. Striking results in terms of producing superior-grade fruits, higher marketable yield, and overall fruit quality were achieved with crop loads of 80 fruits per plant. Staggering the crop regulation from June to September effectively extended the harvesting season to four months, from the last week of November to March, with eventual benefit of reduced fruit cracking compared to the standard two-month harvesting period obtained from normal regulation. Soil application of the indigenously developed OMF-K in two equal splits, along with the recommended N and P through inorganic sources, significantly reduced fruit cracking (only 6.23%) compared to the recommended NPK through inorganic sources (26.9%), while maintaining similar physicochemical quality attributes.

Transcriptome and Metabolome Based Mechanisms Revealing the Accumulation and Transformation of Sugars and Fats in Pinus armandii Seed Kernels during the Harvesting Period.

Pinus armandii seed kernel is a nutrient-rich and widely consumed nut whose yield and quality are affected by, among other things, harvesting time and climatic conditions, which reduce economic benefits. To investigate the optimal harvesting period of P. armandii seed kernels, this study determined the nutrient composition and seed kernel morphology and analyzed the gene expression and metabolic differences of P. armandii seed kernels during the harvesting period by transcriptomics and metabolomics. The results revealed that during the maturation of P. armandii seed kernels, there was a significant increase in the width, thickness, and weight of the seed kernels, as well as a significant accumulation of sucrose, soluble sugars, proteins, starch, flavonoids, and polyphenols and a significant decrease in lipid content. In addition, transcriptomic and metabolomic analyses of P. armandii seed kernels during the harvesting period screened and identified 103 differential metabolites (DEMs) and 8899 differential genes (DEGs). Analysis of these DEMs and DEGs revealed that P. armandii seed kernel harvesting exhibited gene-metabolite differences in sugar- and lipid-related pathways. Among them, starch and sucrose metabolism, glycolysis, and gluconeogenesis were associated with the synthesis and catabolism of sugars, whereas fatty acid degradation, glyoxylate and dicarboxylic acid metabolism, and glycerophospholipid metabolism were associated with the synthesis and catabolism of lipids. Therefore, the present study hypothesized that these differences in genes and metabolites exhibited during the harvesting period of P. armandii seed kernels might be related to the accumulation and transformation of sugars and lipids. This study may provide a theoretical basis for determining the optimal harvesting time of P. armandii seed kernels, changes in the molecular mechanisms of nutrient accumulation, and quality directed breeding.

Spatiotemporal climate variability and food security implications in the Central Ethiopia Region

Studies focusing on the spatiotemporal distribution of climatic parameters and meteorological drought are of paramount significance for countries like Ethiopia, where climate change and variability cause major losses to rain-dependent agriculture. In this study, the National Meteorology Institute of Ethiopia provided an Enhanced National Climate Services (ENACTS) dataset at a spatial resolution of approximately 4 km by 4 km over 38 years (1981–2018) was used to study climate trends, spatiotemporal variability, and meteorological drought in the Central Ethiopia Region. Coefficient of variation (CV), Standardized Rainfall Anomaly (SRA), Standardized Precipitation Index (SPI), Mann-Kendall trend test, and Sen's slope were used for the analysis. The findings suggest that Belg rainfall (also known as "small-rain") varied greatly in space and time over the study area, with area-averaged CV of 29 % and pixel-level CVs ranging from 63 to 93 %. The average precipitation during Belg experienced a 15 % decrease from 2000 to 2019 compared to the preceding two decades, from 1981 to 1999. The maximum temperature has increased significantly during the Annual, Belg, and Bega seasons. The SPI and SRA showed that there have been multiple drought episodes with rising negative rainfall anomalies, with a drought occurring every 2.9 years during the Kiremt (also called "big rain", spanning from June to September) and Belg seasons. The growing negative rainfall anomaly, high CV, and highly significant increase in mean maximum temperature during the Belg season is concerning for food security and poverty eradication. The notable rise in rainfall during the June (sowing period) and November (harvesting period) also hurts crop production during the main cropping season. Thus, developing appropriate adaptation strategies and policies oriented toward climate-resilient agriculture is crucial to meet the global sustainable development goals (SDGs) and Africa Union's Agenda 2063.

Four decades in the vineyard: the impact of climate change on grapevine phenology and wine quality in northern Italy

The wine sector, among the most profitable agricultural segments, has been markedly affected by the ongoing climate change impacts, such as warmer climate conditions with higher frequency of extreme temperatures and a trend of decreasing precipitation. All this results in higher evaporative demand and therefore higher occurrence of water stress events leading to advancement of temperature-sensitive phenological stages (e.g., budburst and ripening). Such negative effects eventually affect berry development and quality, especially in historically valuable viticultural areas, forcing winegrowers to work within a compressed harvest period to maintain wine typicity. In this work we examined the relationship between environmental variables (air and soil temperature, relative humidity, precipitation, and solar radiation), phenology, berry, and wine quality for the two varieties (Chardonnay and Teroldego) in Trentino Alto-Adige/South Tyrol (Italy) over 36 years. Huglin Index (a bioclimatic heat index), growing degree days (measure of heat accumulation), and overall mean temperature showed linear increase (p < 0.001) in the last years, while no variations were recorded for precipitations. Despite no major effects being observed for phenological interval lengths, the onset of most of the phenological stages for both varieties had significantly (p < 0.001) advanced. However, i) early budburst pushed the budburst-flowering interphase by -1.2 days every two years toward putative colder periods with increased late frost probability and potential slower phenological progression towards flowering, and ii) early veraison shifted the veraison-ripening interphase by 0.25 day per year into warmer periods that oppositely impose faster phenological advancement. Hence, a substantial equilibrium in the seasonal growing length over years was maintained. Potential carry-over effects from the previous season were observed, particularly associated with heat requirements to unlock early phenological events, raising additional concerns on the additive effects of climate change to viticulture. Generally, white wine quality increased (p < 0.05) over the years, while red and sparkling wines remained unaffected. This was putatively related to accurate harvest date decision-making dictated by berry quality parameters: sugar-to-acidity ratio for Chardonnay and bunch sanitary status for Teroldego. Overall, this work provides evidence of the dynamics involved in climate change, and, to our knowledge, its overlooked effects on viticulture, thus providing new insights that can contribute to further developing adaptive strategies.

Advancements and Challenges in Personalized Therapy for BRAF-Mutant Melanoma: A Comprehensive Review.

Over the past several decades, advancements in the treatment of BRAF-mutant melanoma have led to the development of BRAF inhibitors, BRAF/MEK inhibitor combinations, anti-PD-1 therapy, and anti-CTLA4 therapy. Although these therapies have shown substantial efficacy in clinical trials, their sustained effectiveness is often challenged by the tumor microenvironment, which is a highly heterogeneous and complex milieu of immunosuppressive cells that affect tumor progression. The era of personalized medicine holds substantial promise for the tailoring of treatments to individual genetic profiles. However, tumor heterogeneity and immune evasion mechanisms contribute to the resistance to immunotherapy. Despite these challenges, tumor-infiltrating lymphocyte (TIL) therapy, as exemplified by lifileucel, has demonstrated notable efficacy against BRAF V600-mutant melanoma. Additionally, early response biomarkers, such as COX-2 and MMP2, along with FDG-PET imaging, offer the potential to improve personalized immunotherapy by predicting patient responses and determining the optimal treatment duration. Future efforts should focus on reducing the T-cell harvesting periods and costs associated with TIL therapy to enhance efficiency and accessibility.

Effects of Cultivar Factors on Fermentation Characteristics and Volatile Organic Components of Strawberry Wine.

Strawberry wine production is a considerable approach to solve the problem of the Chinese concentrated harvesting period and the short shelf life of strawberries, but the appropriative strawberry cultivars for fermentation are still undecided. In this study, the strawberry juice and wines of four typical strawberry cultivars named Akihime (ZJ), Sweet Charlie (TCL), Snow White (BX), and Tongzhougongzhu (TZ) were thoroughly characterized for their physicochemical indicators, bioactive compounds, and volatile organic components (VOCs) to determine the optimal strawberry cultivars for winemaking. The results showed that there were significant differences in the total sugar content, pH, total acid, and other physicochemical indexes in the strawberry juice of different cultivars, which further affected the physicochemical indexes of fermented strawberry wine. Moreover, the content of polyphenols, total flavonoids, vitamin C, and color varied among the four strawberry cultivars. A total of 42 VOCs were detected in the strawberry juice and wines using headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry (HS-SPME-GC-MS), and 3-methyl-1-butanol, linalool, trans-2-pinanol, hexanoic acid, and hexanoic acid ethyl ester were the differential VOCs to identify the strawberry wine samples of different cultivars. Overall, strawberry cultivar ZJ had a relatively high VOC and bioactive compound content, indicating that it is the most suitable cultivar for strawberry wine fermentation. In addition to determining the relatively superior fermentation characteristics of cultivar ZJ, the results may provide a theoretical basis for the raw material quality control and quality improvement of strawberry wine.

Mitigating the effects of extreme weather on crop yields: insights from farm management strategies in the Netherlands

Abstract Weather extremes can drive substantial crop losses. Farm level management strategies play a critical role in mitigating the impacts of and consequences for farmer livelihoods and food security. While the impacts of extreme weather on crop yields are well documented in recent studies, these predominantly focused on expansive geographical scales and commonly overlooked the critical role of management practices in modulating the dynamics of weather-crop sensitivities. In our study, a unique data-set is used that explores the timely relationship between extreme weather and crop yields at farm level in the Netherlands. We cover 10 types of crops and elucidate the role of soil types, irrigation and nutrient application in modulating the relationship between extreme weather and crops. We show substantial impacts from drought during the growing- and harvesting period and excessive precipitation during the planting- and growing period. Severe droughts show significant (p≤0.05) reductions in yield for all crops, and lead to yield reductions up to 24 percent relative to average yields during the growing period. Meanwhile, eight crops show significant reductions in yield due to severe water excess during the planting period, with yield reductions up to 18 percent. Soils such as sand or loess amplify the negative impact of drought on crop yield, while softening the impact of excessive precipitation. Irrigation and to a lesser extent nutrient application are shown to moderately decrease the impact of extreme weather on crop yield. Our findings contribute valuable insights to guide local adaptation priorities which are critical given the projected increase in the intensity and frequency of extreme weather under climate change.

The Status of Beekeeping in Simada District, Amhara, Ethiopia, with Its Challenges and Opportunities

The study was designed to assess the existing beekeeping practices, constraints, and potential of honeybee production in Simada district. The study was carried out in four proportionally selected kebeles of highland, midland, and lowland agro-ecology. Accordingly, a total sample size of 146 beekeepers, depending on their potential, was interviewed using a structured and semi-structured questionnaire. A semi-structured questionnaire, field observation, and focal group discussion were employed to collect primary data. Descriptive statistics such as mean, frequency, and standard deviation were used to analyze the data. The majority of beekeepers in the district are mail-headed, and the majority can read and write. Beekeepers practiced three hive types but mostly used traditional hives. The majority of honeybee colonies are found in midland agro-ecology, but they are not significantly different (P<0.05). About 57.5% of beekeepers obtain their colony through buying, and their colony increases through reproductive swarming. Beekeepers construct both traditional and top-bar hives from the surrounding available material. Frame hives were obtained from GOs on a credit basis. Beekeepers indicated that the majority of honey was harvested in October and November. The second minor harvesting period was from May to June, which depends on the nature of the yearly rainfall conditions. As the respondent's described, they stored honey below one year in a plastic jar, clay jar, and plastic sack when plenty of products were obtained and for medicinal value, unless they used honey during harvesting as a source of income. Predators and pests are major constraints on honey bee production, followed by pesticides and herbicides in the study area. Other identified beekeeping constraints were shown in relative order of importance: drought, death of colony, lack of water, migration, and disease are some of the problems that hinder productivity. Honeybees required feed supplementation during the dry season; about 28% of beekeepers fed their colonies with higher supplements made from February to April. The commonly used supplements were peas and bean flour (Shiro), barley flour (Besso), sugar, honey, and others, including Niger.

Effects of Harvest Time on Medicinal Qualities of Hemp

Harvest time impacts the physicochemical properties of hemp. This study investigated the relationship between harvest time on growth parameters, physiological parameter, and color, in addition to Cannabidiol (CBD), Tetrahydrocannabinol (THC) and Cannabigerol (CBG) concentrations. Siskiyou cannabis was harvested at 5 different stages after flowering 50% at week five to week nine. The rational explanation for stages on growth parameters, physiological parameters, color, and total physiochemical properties was found, while total color changes ranged from brightness (L*) decreased gradually from 66.06 at 5 weeks to a minimum of 16.14 at 9 weeks of flowering. On the contrary, the redness (a*) and yellowness (b*) increased from –0.65 and 8.95 at 5 weeks to their peak values of 19.99 and 34.24 at 9 weeks, respectively. The hue also decreased from 110.03 at 5 weeks to a minimum of 80.14 at 9 weeks, with samples being significantly (p <0.05) different. Tetrahydrocannabinol (THC) was higher than 1 % at week 5, which was lower than their Cannabidiol (CBD) concentrations, reaching 14.35% at week 8. Cannabigerol (CBG) in dried samples reached 2.01% at week 7. The average dry weight of inflorescence per plant peaked at 36.75 g in week 8 and week 9 respectively. Significant differences in Crop Growth Rate (CGR) were noted across the harvesting periods, notably 17.13 and 16.70 g cm⁻² day⁻¹ at weeks 8 and 9, respectively, representing the highest dry weight accumulation per unit area. This increase in dry weight accumulation indicates higher efficiency. Finally, the Harvest Index (HI) showed notable discrepancies among the post-flowering harvesting times, with the greatest total dry weight observed at 0.357 and 0.345 at weeks 8 and 9, respectively. These findings could be of industrial relevance for improving post-harvest processes while maintaining the quality of this regulated crop.

Occurrence pattern of diseases and pests in strawberry greenhouse during harvest period

Occurrence pattern of diseases and pests in strawberry greenhouse during harvest period

Monitoring and zoning soybean maturity using UAV remote sensing

Timely and accurate determination of the maturity status during the soybean harvest period is crucial for devising strategic harvesting plans, significantly enhancing the intelligent management of soybean production and minimizing losses. To explore the maturation dynamics of soybeans and pinpoint the precise maturity stage for optimal harvesting, we collected Unmanned Aerial Vehicle (UAV) remote sensing data, along with physical and chemical parameters, during the Beginning maturity(R7)-Full maturity(R8) stages from 2021 to 2022. We analyzed the variations in these parameters throughout the maturation period. Employing UAV multispectral technology and correlation analysis algorithms, we established a relationship between the spectral and physicochemical parameters during the maturation period, which led to the formulation of a soybean maturity evaluation index. Furthermore, we incorporated the concept of precision management zoning. In line with actual field production requirements, this index, combined with real-time moisture content, established a standard for grading soybean maturity. Additionally, our study examined the impact of spatial scale on maturity assessments by conducting a theoretical analysis of regionalized variables and analyzing the spatial correlations and variability of soybean maturity across different field plots. We determined the optimal theoretical model for various sampling distances, thereby establishing feasible zoning ranges. Comparative analysis of zoning algorithms, including Hierarchical Clustering (HC), K-Means, and its optimized versions K-Means++ and Mini Batch K-Means, was conducted using the partition performance index as the criterion. This analysis identified the most suitable algorithm for assessing soybean field maturity, which was subsequently verified through field tests. Results indicated that soybean leaf chlorophyll content and photosynthesis decreased sharply initially, then stabilized at a low rate. Water content in both soybean plants and seeds decreased rapidly before slowing, with plant dehydration rates exceeding those of the seeds. Green Normalized Difference Vegetation Index(GNDVI) showed a positive correlation with various physiological indicators, and soybean maturity was classified into three levels using the unit gridding management method, considering actual moisture content. Spatial correlation analysis of maturity variations in small and large test fields revealed Moran indices ranging from 0.78 to 0.95 and 0.70–0.90, respectively. Optimal detection ranges were determined to be 2.00–30.00 m for small fields and 2.00–67.00 m for large fields. The zoning categories for small and large fields were three and four, respectively. Comparative studies showed that the Mini Batch K-Means algorithm, an optimization of K-Means, achieved Calinski-Harabasz Index(CH) scores and silhouette coefficients of 10521.97, 109508.27, and 0.57, 0.54, respectively, indicating comparable zoning effectiveness to K-Means and K-Means++ and superiority over the HC algorithm. The zoning speeds were 12.50 s and 191.68 s, respectively, underscoring its efficiency in maturity zoning. Furthermore, zoning results corresponded well with actual soybean maturity. Terrain had little impact on soybean maturity, which was more susceptible to slope aspect. This study provides a theoretical foundation and practical guidelines for applying UAV remote sensing technology in determining soybean maturity and optimal harvesting periods, thereby reducing potential harvest losses.

Addressing local sparsity in massive agricultural machinery trajectories: A BiLSTM-GRU approach

Trajectory data acquired from GNSS (Global Navigation Satellite System) terminals on agricultural machinery are crucial for identifying agricultural machinery operation modes, evaluating agricultural machinery operational efficiency and exploring agricultural machinery trans-regional harvesting operation characteristics. However, GNSS terminals often experience signal delays due to factors such as weather conditions and environmental obstructions. These delays result in irregular time intervals between trajectory points, leading to local sparsity within the trajectory data, which subsequently reduces the accuracy of applications and analyses based on agricultural machinery trajectories. To address this issue, we propose a novel approach that leverages Bidirectional Long Short-Term Memory (BiLSTM) and Gated Recurrent Unit (GRU) networks, along with an attention mechanism, to mitigate the problem of local trajectory sparsity, and experiments were conducted using agricultural machinary trajectory data collected during the 2023 wheat harvest period. The results demonstrate the efficiency of our approach by successfully resolving the local sparsity of agricultural machinery trajectories. Moreover, each newly added trajectory point contains all original attributes (e.g., speed and direction). When integrated into state-of-the-art algorithms (e.g., DT, DBSCAN + rules, GCN) for identifying machinery operation modes, our method improves accuracies by 21.83 %, 26.86 %, and 1.17 %, respectively. Our approach effectively addresses the issue of local trajectory sparsity, thus providing assistance for applications and studies based on massive agricultural machinery trajectories.

Determination of Some Antioxidant Activity Values in Wines of Vitis vinifera L. Karalahna, Karasakız and Çavuş Grape Varieties Produced in Bozcaada

Vinifera linné subsp., whose traditional names are Karalahna, Çavuş and Karasakız, in Çanakkale Bozcaada region, which has witnessed different cultures and periods for centuries due to its strategic location. It is known that wines made from grapes belonging to vinifera species have been consumed by local people for years. Wine is a traditional fermented beverage rich in phenolic substances and antioxidant properties, where product is kept in red wine with its skins, especially during maceration stage, due to production process. Phenolic content and antioxidant properties vary depending on grape variety, and these properties are important for human health. In this study, 2,2-diphenyl-1-picryl-hydrazyl (red wine), çavuş (white wine) and karasakız (red wine) wines produced from karalahna, karasakız (kuntra) and çavuş grapes of the 2020-2021 harvest period were examined. DPPH• free radical scavenging activity, total phenolic substance (Folin-Ciocalteu), Fe+3 reducing power, total flavonoid, anthocyanin, condensed tannin properties were examined.

Effect of harvesting time on root yield and nutritional composition of orange-fleshed sweet potato [Ipomoea batatas (L.) Lam] varieties in East Hararghe

In eastern Ethiopia, sweet potato is a vital food and nutrition security crop; moreover, orange-fleshed sweet potato (OFSP) varieties are rich in beta-carotene content and have the potential to alleviate chronic Vitamin A malnutrition in the region. However, the unavailability of adaptable varieties and lack of information on production and post-harvest handling practices have limited its production and utilization in eastern Ethiopia. The research was conducted to identify the proper harvesting stage of OFSP varieties for optimum yield and nutritional compositions at Rare and Babile research stations of Haramaya University during the main rainy season of 2022. Three varieties (Alamura, Kabode, and Bakule) and four harvesting periods (120, 150, 180, and 210 days after planting (DAP) in factorial combinations were evaluated in randomized complete block design with three replications. Data were collected for growth, yield, and physicochemical composition-related parameters. Combined analysis of variance revealed the interaction effect of harvesting time and varieties had a significant (p < 0.05) effect on yield, yield-related parameters, and physicochemical components. Alamura variety produced comparable above-ground biomass (28.99 t ha−1) and the highest marketable root yield of 36.40 t ha−1 at 150 DAP, with dry matter content of 33.01 and 30.58 % at 150 and 120 DAP, respectively. Harvesting Alamura at 150 DAP also had the highest ꞵ-carotene, zinc, and iron contents of 11809 μg/100 g, 3.79, and 14.47 mg/100 g, respectively. It was concluded that growing the Alamura variety and harvesting at 150 DAP was better for obtaining higher root yield with good nutritional compositions in the study area.

Minimisation of losses of consumer quality during storage of cherry fruits under the influence of hydrocooling and protective composition

The need for consumption of fruit products, in particular sweet cherries and cherries, is growing every year in Ukraine. This is driven by national traditions, stakeholder needs, and the population's demand for healthy eating. Cherries are a nutritious fruit that contains such functional ingredients as essential amino acids, vitamins, minerals, dietary fiber, etc. Unfortunately, in terms of long-term storage, cherries quickly lose their quality and have a short harvest and processing period. To increase the shelf life of products, it is advisable to develop and improve methods of storage and processing of cherries. Optimisation of chilling methods for cherries before storage increases the shelf life of fresh fruit and the suitability for fresh consumption. Improving the methods of chilling cherries will help to increase the demand for its fruits by all stakeholders in the European market. The aim of the research was to establish the optimal concentration of acetic and lactic organic acids, which are included in the protective composition for processing cherry fruits, and to determine the level of daily losses of cherry fruits during hydrocooling while maintaining the global optimum point. To establish an effective cooling method, fruit losses caused by diseases of microbiological origin and physiological disorders were determined. During storage, the effect of the concentrations of the protective composition on the indicators of weight loss and the average level of daily fruit loss was studied. A regression model was built and the optimal values of the indicators were determined by stages: a regression model was built on the basis of experimental data and the global optimum point was determined on the basis of the model and a confidence interval for the optimum was built. According to the results of the research, the lowest rate of daily fruit losses at the level of 0,117%-0,121% was recorded during hydrocooling of cherry fruits using organic compositions of lactic and acetic acids in the ratio of their components of 1,25-2,25:1,50-2,25 and 1,75-2,25% with the amount of acetic acid at 1,25%, respectively. The regression analysis showed the optimal value of the average level of daily losses during storage of cherry fruits of the model variety ‘Vstrecha’ – 0,106% at the value of factors x1=2,217 and x2=1,966.

Extraction, identification and mass production of arbuscular mycorrhizal fungi (AMF) from faba bean (Vicia faba L.) rhizosphere soils using maize (Zea mays L.) as a host plant

Ethiopia is the second-largest grower of faba bean in the world next to China. The crop is highly useful with its edible seed serving as an essential protein complement of the Ethiopian diet, especially for those who cannot afford animal protein. Even though faba bean is mycotrophic to Arbuscular Mycorrhizal fungi (AMF), the different genera and species that are associated with the crop are not determined in yet at the maturity time of the crop (harvesting period). Sixteen faba bean rhizospheric soils were collected to isolate and identify AMF. Spores were extracted using the wet-sieving and decanting method. The Glomus genera was the most dominant, followed by the Acaulospora and Gigaspora genera. The highest spore load per 100 g of soil was observed in the sample that contained the lowest soil phosphorus. Furthermore, an inverse relationship between the spore load and soil phosphorus was observed. Three treatments were considered for mass multiplication of AMF, viz, Treatment (1) Glomus aggregatum, treatment (2) Glomus sp.BZ, and treatment (3) Glomus sp.AZ. However, the highest number of AMF's spore and root colonization was seen in treatment 3 with significant difference (P < 0.05) from the others. In conclusion, AMF constituted an important component of the faba bean rhizosphere during its harvesting period (dry season) and its multiplication using maize favored the viability and infectivity of the fungi.

Do nanoparticles and colloids replenish soil phosphorus in the rhizosphere of winter wheat?

The rhizosphere is generally depleted in nutrients, but as a hotspot of microbial activity it fosters crop P uptake. We hypothesized that P contents of water extractable nanoparticles (<0.1 μm) and small sized colloids (<0.45 μm) differ between non-rhizosphere and rhizosphere soil. To test this hypothesis, rhizosphere and non-rhizosphere soils (Luvisol and Cambisol) were sampled at harvest period of winter wheat near Selhausen (Germany). Microaggregate and colloidal fractions in the size range of 53–250 μm, 20–53 μm, 0.45–20 μm, and <0.45 μm were separated by wet-sieving and centrifugation. Subsequently, the colloids <0.45 μm were further isolated in 0.66–20 nm, 20–100 nm and 100–450 nm fractions using asymmetric flow field flow fractionation (AF4) and directly analyzed by online coupled organic carbon detector (OCD) and inductively coupled plasma mass spectrometry (ICP-MS) for element composition. No significant differences (p > 0.05) were measured between rhizosphere and non-rhizosphere soil P contents of microaggregate fractions. The rhizosphere soil, however, showed ∼26 % depletion of average P content in the 0.66–20 nm fraction, which went along with an enrichment of P content of the 100–450 nm fraction by a factor of two. Apparently, P uptake by plants results in a redistribution of P in the rhizosphere, with small nanoparticles providing available P to plants while excess residual P is bound to fine colloids.