Vegetation condition has declined along many regulated river systems globally due to alteration of flow regimes via flow regulation. Understanding how plants respond to inundation is critical for managing regulated river flows to improve riparian vegetation condition. We experimentally tested the effects of inundation duration on the survival and growth of six tufted grass species commonly found in riparian zones in south-eastern Australia. We conducted three nursery-based experiments in late winter/early spring, corresponding with natural temperate flow peaks and managed flows, with inundation treatments on: (1) established plants, up to 35 days inundation; (2) seedlings, up to 25 days inundation; and (3) established plants, up to 53 days inundation including additional shaded treatments. Plant survival, height and biomass growth, and onset of flowering were recorded for established plants, and, for seedlings, survival only. Plant height and biomass growth declined with longer inundation duration across all species, although surprisingly few established plants died. Unexpectedly, grass seedlings were generally tolerant of inundation also, although there was some evidence of increased mortality for the longest treatment (25 days inundation). Shading did not result in increased mortality or reduced height growth of inundated plants. Inundation effects on the onset of flowering were modest and varied between species ranging from earlier to delayed onset. Our results suggest that tufted grasses are tolerant of cool-season inundation. Given that mortality of these species has been observed in the field and experimentally under shorter inundation periods in warmer conditions (late spring and summer), we suggest that seasonal timing of inundation is critical in determining plant responses to inundation.