Periodic acid-Schiff Technique Research Articles

Some histochemical observations on periodic acid-amino-acid mechanisms.

THE periodic acid Schiff technique has been used histochemically primarily as a means of localizing the 1,2 glycol linkage in tissue sections1. It is known, however, that periodic acid is also capable of cleaving the 1,2 hydroxylamino linkage with the production of some aldehyde radicals, and although the exact reaction mechanism is not understood, it is believed by some to react in a somewhat similar manner with a number of amino-acids possessing structurally neither of these linkages. The amino-acids serine and threonine, as well as the “hydroxylysine” of Van Slyke, fall into the first category, whereas cystine, methionine, tryptophane, glycine, alanine, tyrosine, histadine, aspartic and glutamic acids fall into the latter group2. This investigation was initiated to study specifically this phase of the periodic acid Schiff mechanism, and although the results were not conclusive, it was felt that a brief description of the experiments might be of interest to others working in this particular field.

Osteogenesis imperfecta congenita: A connective tissue diathesis

<h2>Summary</h2> A case of osteogenesis imperfectain a newborn infant has been reported. The failure of reticulum to differentiate into mature collagen has been demonstrated in the corium, sclera, and cornea. The presence of a material in the bones which stains intensely with the periodic acid-Schiff technique and which may be an immature form of bone matrix has been described.