This study aimed to assess the role of dipyrone, in association with flunixin meglumine (FLU), in promoting a more effective modulation of physiological stress indicators during the postoperative period in ewes submitted to surgical embryo collection. Ewes were allocated into either flunixin (G-FLU; n = 11) or flunixin and dipyrone (G-FLUDIP; n = 11) group after laparotomy: All ewes received 2 mg/kg FLU and the G-FLUDIP received additionally three doses of 50 mg/kg dipyrone intramuscularly immediately, 24 h, and 48 h after the surgery. Embryo recovery by laparotomy had an average duration of 24 ± 0.85 min. The G-FLUDIP-ewes had a shorter interval to eat compared to G-FLU-ewes (9.5 ± 0.3 vs. 12.4 ± 0.4 min; P = 0.001). Serum cortisol concentrations presented a tendency (P = 0.07) of treatment×time interaction. The interaction effect is immediately after surgery and the G-FLU-ewes had a higher concentration of cortisol compared to G-FLUDIP-ewes (P = 0.002). For glycemia, a treatment×time interaction was observed (P = 0.04), but overall, concentrations were similar between the treatments (P = 0.31). There was a treatment×time interaction in serum globulins (P = 0.04), in which G-FLU had greater time to increase those concentrations than G-FLUDIP. The G-FLUDIP-treated ewes showed a lower (P = 0.01) overall platelet count compared to G-FLU. A treatment effect was also observed in overall lymphocyte (P = 0.01) and monocyte counts (P = 0.03). In conclusion, the association of flunixin and dipyrone after surgical embryo collection may promote a more effective modulation of pain and inflammation in ewes since these animals had lesser stress in the initial 24-h period after the procedure. This association was shown to decrease cortisol levels, promote more effective control of thrombocytosis, reduce the acute inflammation recovery time in the ewes, and reduce the interval of time that it took the ewes to eat after the procedure. Thus, the association of both flunixin and dipyrone may be important to increase ewes’ welfare after surgical embryo collection.