Peri-urban Streams Research Articles

The impact of stormwater management strategies on the flow regime of a peri-urban catchment facing urbanisation and climate change: a distributed modelling study in Lyon, France

ABSTRACT The relative impact of urbanisation (urban sprawl), climate change (rainfall and evapotranspiration) and stormwater disconnection strategies (implementation of green infrastructure (GI)) on a peri-urban stream in the western suburbs of Lyon, France, was investigated using a distributed, physically based model (J2000P). Results showed that ambitious catchment-scale implementation of GI has the potential to mitigate some hydrological effects of urbanisation, especially a drastic reduction of combined sewer overflows. However, GI did not have a large impact on the stream flow regime, especially in the context of climate change, as the stream is dominated by non-urban contributions. Spatially distributed models have the capacity to inform river managers about the extent of implementation of GI needed to achieve given management goals and highlight some limitations of disconnection strategies. Indeed, while GI improved the resilience of sewer networks to global change, it was not sufficient to compensate for the effects of climate change on streamflow.

Aspectos reprodutivos do camarão Desmocaris trispinosa (Aurivillius, 1898) em um riacho suburbano de Abidjan (riacho Banco, Costa do Marfim)

Abstract Aim Characterize habitats and determine the reproductive parameters of the prawn Desmocaris trispinosa (Aurivillius, 1898) from Banco stream, a periurban stream of Abidjan (Côte d'Ivoire, West Africa). Methods Sampling was conducted monthly, from July 2020 to June 2021 at four stations. Environmental characteristics were measured before prawn sampling. The sampling was made with a dipnet during five-minute active fishing conducted by a single operator at each station. Results According to environmental variables, sampling stations were divided into three groups: almost undamaged (B4), slightly disturbed (B1) and disturbed (B2 and B3). In total, 467 specimens of D. trispinosa were captured in which 127 males, 275 females and 65 juveniles. Overall, the sex ratio (1:2.1) was in favour of females. Considering stations, the sex ratio was also in favour of females. But, only in the almost undamaged station (B4), the difference was significant (χ2 = 70.20; P<0.001). At this station, a variation of sex-ratio was observed in two seasons. In total, 116 ovigerous females were analysed on all stations. Fecundity varies from 5 to 16 oocytes with an average of 11.03 ± 2.18. First maturity size was estimated at 4.82 mm carapace length for the combined data. Females from the station free from human activities (B4) reached sexual maturity (4.23 mm) before those of the other stations (4.97 in B1 and 5.70 mm in B2). D. trispinosa from Banco stream reproduces continuously year-round with two peaks in the rainy season. The oocyte volume was negatively influenced by temperature and positively by dissolved oxygen. Conclusions D. trispinosa reproduces year-round in undisturbed stations and reaches sexual maturity faster at these stations compared to disturbed stations. The sex ratio was in favour of females. Consequently, this species would be naturally polygamous.

Data-Driven System Dynamics Model for Simulating Water Quantity and Quality in Peri-Urban Streams

Holistic water quality models to support decision-making in lowland catchments with competing stakeholder perspectives are still limited. To address this gap, an integrated system dynamics model for water quantity and quality (including stream temperature, dissolved oxygen, and macronutrients) was developed. Adaptable plug-n-play modules handle the complexity (sources, pathways) related to both urban and agricultural/natural land-use features. The model was applied in a data-rich catchment to uncover key insights into the dynamics governing water quality in a peri-urban stream. Performance indicators demonstrate the model successfully captured key water quantity/quality variations and interactions (with, e.g., Nash-Sutcliff Efficiency ranging from very good to satisfactory). Model simulation and sensitivity results could then highlight the influence of stream temperature variations and enhanced heterotrophic respiration in summer, causing low dissolved oxygen levels and potentially affecting ecological quality. Probabilistic uncertainty results combined with a rich dataset show high potential for ammonium uptake in the macrophyte-dominated reach. The results further suggest phosphorus remobilization from streambed sediment could become an important diffuse nutrient source should other sources (e.g., urban effluents) be mitigated. These findings are especially important for the design of green transition solutions, where single-objective management strategies may negatively impact aquatic ecosystems.

Spatial distribution of pharmaceuticals within the particulate phases of a peri-urban stream

Pharmaceutical products (PPs) are consumed worldwide and are continuously released into hydrological environments, but are not efficiently removed by sewage treatment plants. Their occurrence within the dissolved phase has been extensively studied, but only a few articles concern solid matrices. The mechanisms and extent of sorption depend on the properties of both the molecules (degradability, charge, hydrophobicity) and the matrices (clay content, organic matter content), making the spatio-temporal distribution of PPs in natural environments complex and poorly elucidated. To improve our understanding of PP distribution at a catchment scale, this study investigated different groups of molecules with varying solubility and charges, in water, suspended particulate matter, bed-load and pond sediments. The Egoutier stream, which collects the sewage effluents from two health institutions sewage effluents, is a good candidate for this investigation. Results indicate that PP occurrences in the different particulate compartments were mainly regulated by their wastewater occurrences and charges. Particulate phases all along the Egoutier stream were characterized by a limited clay content (i.e. less than 1%) and significant organic carbon content (i.e. between 0.3% and 18.0%) favouring non-specific adsorption. Therefore, neutral PPs, exhibiting higher discharge rates, persistence and hydrophobicities in comparison with cationic and anionic molecules, were the most abundant PPs in the particulate phases of this catchment. In bed-load sediments, global PP spatial distributions reflected discharge sites and sedimentary accumulation zones, mostly that of organic matter. Spatial distributions of the more hydrophobic and persistent PP in the particulate phases thus followed the stream sedimentary dynamic.

Evaluating the ecological status of mountain streams from a megacity (Mexico City) with diatoms: development and implementation of morphological and eDNA-based methods

In central Mexico, streams flow from mountain protected areas at high elevations, i.e. abive 2,400 m a.s.l., being exposed to different human activities along their course. The main human threats of these aquatic ecosystems include the presence of hydraulic infrastructure (e.g. canals and dams), the construction of bridges or roads, fish farming, agriculture, livestock, unregulated tourism or irregular human settlements, resulting in nutrient enrichment, organic pollution and low hydromorphological quality. Diatoms, widely recognized as valuable indicators of environmental conditions, offer a unique opportunity to assess the ecological quality of the streams in and around this megacity, as they respond directly and sensitively to specific physical, chemical and biological changes. To conduct ecological assessment, the composition of diatom communities has traditionally been characterized by morphological methods and most recently by environmental DNA (eDNA) metabarcoding. However, the diatom communities of only a few of these streams in the Basin of Mexico have been characterized morphologically at low resolution light microscopy (LM), and no eDNA-based studies have been conducted yet. In this context, our aims are to 1) implement morphological and eDNA metabarcoding methods for the identification and quantification of epilithic diatoms and to assess their seasonal and spatial response in three peri-urban mountain streams from the Basin of Mexico, and 2) develop a metric to assess and monitor the ecological quality in these streams. For this purpose, we characterized a) the physical, environmental and hydro-morphological conditions related to ecosystem quality, and b) the diatom diversity and community structure of nine sites per stream (27 sites). The sites include headwaters, as well as middle and lower reaches with different land-use and anthropologic pressures, and were investigated over three hydrological periods, i.e. post-rainy, dry-cold and dry-warm. Species identification was first conducted under LM and scanning electron microscopy (SEM), and is currently been done by eDNA metabarcoding of the V4 region of the 18S rRNA gene and a fragment of the chloroplast marker rbcL. Our study has resulted in 520 infrageneric taxa identified by LM and SEM, the largest diversity ever found for freshwater diatoms of Mexico. From this data, we found a clear environmental gradient from almost pristine conditions towards heavily polluted states as the streams enter the city. These changes are well reflected by variations in the diatom community structure, as revealed by the Diatom Ecological Quality Index (DEQI), newly developed to evaluate the ecological quality of the streams in and around this megacity.

Development of the Diatom Ecological Quality Index (DEQI) for peri-urban mountain streams in the Basin of Mexico.

In the Basin of Mexico, one of the most important economic regions in the country with over 22 million inhabitants, peri-urban streams have been transformed into sewers, drains, and pipes to avoid flooding or unsanitary conditions; the change affects not only the ecosystem's goods and services but also the aquatic communities that support the natural ecological processes. We aimed to develop a diatom-based diagnosis of the ecological quality of these aquatic ecosystems through the study of epilithic diatom response to regional environmental gradients. Samples of epilithic diatoms and water were collected in 45 sites representing 12 perennial streams, and multivariate analyses were performed on environmental and biological data. The ecological quality gradient to which diatoms responded was related to oxygen saturation, soluble reactive phosphorous, dissolved inorganic nitrogen, and hydromorphological quality. Three species groups were recognized according to their ecological preferences along CCA1 axis, indicators of high and low ecological quality, and tolerant species. By assigning an indicator value to each species group, we calculated the DEQI using the formula adapted from Pantle and Buck, indicating five different ecological quality classes. This index is proposed for complementing the ecological quality evaluation as a biological metric that responds to multiple regional stressors of the structure and function of these peri-urban streams in the Basin of Mexico.

Relative influence of watershed and geomorphic features on nutrient and carbon fluxes in a pristine and moderately urbanized stream

Streams are important sites of elemental transformations due to the relatively high contact rates between flowing water and biogeochemically reactive sediments. Increased urbanization typically results in higher nutrient and carbon (C) inputs to streams from their watersheds and increased flow rates due to modification in channel form, reducing within stream net retention and increasing downstream exports. However, less is known on how moderate urbanization might influence the joint processing of C, nitrogen (N), and phosphorus (P) in streams or the relative influence of changes in watershed and stream features on their fluxes. In this study, we performed mass-balances of different C, N, and P species in multiple reaches with contrasting land use land cover and geomorphic features (pools, riffles, runs) to determine the effects of geomorphology versus human influence on elemental fluxes in a pristine and a semi-urban stream. N was the most responsive of all elements, where nitrate concentrations were 3.5-fold higher in the peri-urban stream. Dissolved organic carbon was only slightly higher in the peri-urban site whereas total P not significantly different between streams. In terms of fluxes, nitrate behaved differently between the streams with net retention occurring in the majority of the reaches of the pristine site, whereas net export was observed in all of the reaches of the semi-urban one. We found a decrease in nitrate concentrations with an increase in excess deuterium of the water (d-excess), an indicator of how overall water retention capacity of the watershed favored N loss. Within the stream, the presence of pools, and reduced channel slope, which also increase water retention time, again favored N loss. Overall, nitrate was the most sensitive nutrient to slight urbanization, where higher export to stream was influenced by land use, but where geomorphic features were more important in driving retention capacity.

Microbial Source-Tracking Reveals Origins of Fecal Contamination in a Recovering Watershed.

Fecal contamination of waterbodies due to poorly managed human and animal waste is a pervasive problem that can be particularly costly to address, especially if mitigation strategies are ineffective at sufficiently reducing the level of contamination. Identifying the most worrisome sources of contamination is particularly difficult in periurban streams with multiple land uses and requires the distinction of municipal, agricultural, domestic pet, and natural (i.e., wildlife) wastes. Microbial source-tracking (MST) methods that target host-specific members of the bacterial order Bacteroidales and others have been used worldwide to identify the origins of fecal contamination. We conducted a dry-weather study of Onondaga Creek, NY, where reducing fecal contamination has been approached mainly by mitigating combined sewer overflow events (CSOs). Over three sampling dates, we measured in-stream concentrations of fecal indicator bacteria; MST markers targeting human, ruminant, and canine sources; and various physical–chemical parameters to identify contaminants not attributable to CSOs or stormwater runoff. We observed that despite significant ruminant inputs upstream, these contaminants eventually decayed and/or were diluted out and that high levels of urban bacterial contamination are most likely due to failing infrastructure and/or illicit discharges independent of rain events. Similar dynamics may control other streams that transition from agricultural to urban areas with failing infrastructure.

Impact of Climate Variability and Landscape Patterns on Water Budget and Nutrient Loads in a Peri-urban Watershed: A Coupled Analysis Using Process-based Hydrological Model and Landscape Indices.

Nutrient discharge into peri-urban streams and reservoirs constitutes a significant pressure on environmental management, but quantitative assessment of non-point source pollution under climate variability in fast changing peri-urban watersheds is challenging. Soil and Water Assessment Tool (SWAT) was used to simulate water budget and nutrient loads for landscape patterns representing a 30-year progression of urbanization in a peri-urban watershed near Tianjin metropolis, China. A suite of landscape pattern indices was related to nitrogen (N) and phosphorous (P) loads under dry and wet climate using CANOCO redundancy analysis. The calibrated SWAT model was adequate to simulate runoff and nutrient loads for this peri-urban watershed, with Nash-Sutcliffe coefficient (NSE) and coefficient of determination (R2) > 0.70 and percentage bias (PBIAS) between -7 and +18 for calibration and validation periods. With the progression of urbanization, forest remained the main "sink" landscape while cultivated and urban lands remained the main "source" landscapes with the role of orchard and grassland being uncertain and changing with time. Compared to 1984, the landscape use pattern in 2013 increased nutrient discharge by 10%. Nutrient loads modelled under wet climate were 3-4 times higher than that under dry climate for the same landscape pattern. Results indicate that climate change could impose a far greater impact on runoff and nutrient discharge in a peri-urban watershed than landscape pattern change.

Impacto de la contaminación orgánica sobre el fitoplancton de un arroyo de la llanura pampeana

Fil: Padulles, Maria Luz. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biologia Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biologia Experimental y Aplicada; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Ciudad Universitaria. Instituto de Ecologia, Genetica y Evolucion de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecologia, Genetica y Evolucion de Buenos Aires; Argentina

Dynamics of toxicity within different compartments of a peri-urban river subject to combined sewer overflow discharges

Combined Sewer Overflows (CSO) in small peri-urban streams and rivers are potentially toxic for their biocenosis. Improving the management of CSO discharges requires better knowledge of their dynamics and toxicity. In view to characterizing this toxicity, we sampled the different compartments (benthic and hyporheic zone) of a peri-urban stream located near the city of Lyon in France. The samples were taken at different distances from a CSO and at three period characteristic of different hydrological conditions. Their toxic effects were assessed by bioassays on the dissolved fraction (D. magna, V. fisheri and B. calyciflorus bioassays) and on the particle fraction (V. fisheri and H. incongruens bioassays). The results highlighted significant toxicity of the particulate fraction for the benthic and hyporheic samples, in particular downstream of the CSO, but with high spatio-temporal variability. This variability can first be attributed to the variability of CSO discharge sampling as a function of season and rainfall, and the dynamics of polluted particles (trapping of transported particles in infiltration zones, mobilization during floods). These parameters play a fundamental role in the distribution of pollutants according to the geomorphology of stream facies. Regarding dissolved pollutants, the chemical exchanges taking place at the “water-sediment” interface trigger the transfer of pollutants from one phase to another, after which the dispersion of these pollutants is governed by hydraulic flows. Finally, critical zones and periods are identified for the peri-urban river toxicity studied: benthic sediments under mean flow downstream; hyporheic sediments after a storm event downstream, close to the CSO. Recommendations are made on the basis of the knowledge obtained to optimize the management of these discharges.

Characterization of Chironomidae (Diptera) surface-floating pupal exuviae sample sort time from coastal tropical aquatic systems.

Many studies either ignore chironomids or only identify specimens to subfamily or tribe due to the associated difficulty and high cost with processing and identifying larvae. An efficient form of sampling chironomids involves collections of surface-floating pupal exuviae (SFPE). SFPE sample sorting has been shown to be more time efficient than traditional dip-net methods in temperate urban and peri-urban streams. However, no published studies have tested the time efficiency of SFPE sample sorting from coastal tropical aquatic systems. We calculated sort times for SFPE samples collected from a coastal tropical stream and an estuary and used multiple linear regression analysis to quantify the relationship between sample sort time and number of specimens, average body length of specimens, and dry weight of sample residue. The average amount of time required to sort very small samples was 69.3min, while moderate samples averaged 85.6min and large samples averaged 153.5min. However, on average, small samples were nine times more time consuming per specimen than large samples. Additionally, dry weight of small-sized residue and the number of specimens contributed significantly to sort time. Therefore, we recommend collecting larger samples, which can be achieved by sampling for 20min over 200-m reaches for stream sites and 500- to 1,000-m reaches for riverine and estuarine sites. Also, we suggest collecting during periods of low wave action and disturbance by boat wake to reduce the amount of sample residue. This research will enhance project planning and budgeting of future studies using the SFPE method to monitor coastal tropical aquatic systems.

Hydrological evaluation of a peri-urban stream and its impact on ecosystem services potential

Rivers are aquatic systems with a unidirectional flow. These systems are highly diverse habitats that support a great variety of organisms, which vary in shape and function, and sustain a diverse range of hydrological ecosystem services (HESs). The HESs provided by rivers varies based on complex hydro-geomorphological dynamics and their relationship with the functional processes of the basin. Land use changes in transition zones, where ecosystem functions are compromised, affect the basin, especially basins close to or on the periphery of urban areas. Such is the case for Mexico City, where 60 m3 of water is consumed per second, 30% of which is imported from outside sources.The rivers of the Magdalena–Eslava sub-basin are among the few remaining surficial water sources in Mexico City. These rivers are located in an area classified as a Soil Conservation Zone, which has been intensely managed for decades. The aims of this paper are (1) to perform a hydrological evaluation of two urban streams and identify their relationship with the provision of hydrological ecosystem services via (i) a hydraulic balance analysis, (ii) a hydro-geomorphological characterization of each stream, (iii) an estimate of present and potential hydraulic erosion, (iv) the determination of physicochemical and bacteriological parameters and (v) a description of macroinvertebrates, macroalgae and their habitats in order to (2) identify the impacts of socio-economic dynamics on the responses of this rural-urban lotic system. Our results show that water flow, forest cover and hydro-geomorphologic heterogeneity are key to sustaining ecosystem functioning, especially in the high and middle sections of the basin. The highest potential provision of water for direct use was recorded in the sub-basin’s middle section; however, the stream channels in that section have lost their natural water flow due to a water management infrastructure built to regulate flow during the rainy season. This intervention can be viewed as a regulation of HESs as water management infrastructure alters the transport of sediment and reduces available natural habitat. The provision of quality water in the lower area of the sub-basin has been seriously compromised by the establishment of illegal urban settlements. A relationship between biologically diverse ecological traits and their response capabilities was established and can be considered an indicator of current HES potential. Therefore, this sub-basin may constitute an example of good management and maximizing potential HESs in an urban-rural setting based on improved management strategies that could be applied in other developing nations.

Diet and feeding selectivity of a benthivorous fish in streams: responses to the effects of urbanization

This study evaluated the diet and feeding selectivity of the catfish Imparfinis mirini in streams with different degrees of urbanization and the effect of rainfall on the availability of prey. The diet was based especially on Chironomidae and Trichoptera. Significant spatial differences in diet were found between the streams; the diet of the fish was similar in the rural and peri-urban streams, and differed from that in the urban stream. Seasonality was an unimportant factor affecting the species' diet, which did not differ significantly between the rainy and dry periods in any of the streams. Fish from the urban stream fed more according to what was available in the environment, while fish from the peri-urban and rural streams showed higher degrees of selectivity. The results indicate that environmental conditions influenced the diet and prey selection of this species in response to the differences in diversity and abundance of the food organisms. They indicate that trophic studies of benthivorous fishes, such as I. mirini, may represent an alternative way to assess human effects on streams.

Nitrogen Changes between Rural and Peri-Urban Stream Subsurface Waters (Yzeron Stream, France)

Urbanization subjects stream and groundwater to increased loads of organic nitrogen, nitrate, and ammonium. Therefore, studying nitrogen species at the urban stream-aquifer interface is important for water resource management. We report here results on water 18O/16O ratios and on nitrogen species in stream subsurface waters upstream and downstream of several combined sewer overflows (CSOs) in a rural area and peri-urban area, respectively. Water 18O/16O ratios were measured to trace the mixing of subsurface waters with stream channel water. Organic nitrogen (ON) and carbon (OC) slightly increased between rural and peri-urban environments in the cold season, but not in the warm season. The highest nitrate levels were observed in rural subsurface waters in the cold season. The lowest nitrate levels were found in peri-urban subsurface waters in the warm season; they corresponded to slow exchange of subsurface waters with channel water.

Age differential response of Hyalella curvispina to a cadmium pulse: Influence of sediment particle size

In Argentina periurban streams frequently receive agricultural, livestock and industrial discharges. Heavy metals have been found in the water column and sediments of numerous water bodies of the pampean region, at levels above the limits established for aquatic life protection. This study aimed to evaluate the effect of a contaminant pulse of cadmium discharged into a water-sediment system of different particle sizes, by means of laboratory tests using juveniles and adults of Hyalella curvispina, a native amphipod. We found that the substrate particle size was a determining factor in the toxicity of cadmium and that the adults of H. curvispina were more sensitive than juveniles. We also observed a temporal difference between the two ages for the same type of sediment. Given the nature of the sediments of regional water bodies, it is expected that a discharge of cadmium, even at concentrations as low as those tested here, will affect the survival of native amphipods.

Multi-Level Approach of the Ecotoxicological Impact of a Combined Sewer Overflow on a Peri-Urban Stream

In periurban zones, urban wet weather discharges have been recognized as the most significant vector of pollution in aquatic environments. The discharge of this water without treatment into the aquatic environment could present an ecotoxicological risk for biocenosis. The aim of the INVASION project is to assess the potential ecotoxicological impact of a combined sewer overflow (CSO) on a peri-urban stream. A comparative study between upstream and downstream areas of the CSO allowed observing significant effects of this overflow on the river. We studied three layers of stream: surface water, benthic layer and hyporheic layer. To characterize the potential ecotoxicological risk of water and sediments, we used a battery of 4 bioassays: Daphnia magna, Vibrio fischeri, Brachionus calyciflorus and Heterocypris incongruens. In parallel, we measured the physico-chemical parameters: ammonium (NH4+), chromium (Cr), copper (Cu) and lead (Pb). An ecological risk is greatest for the hyporheic zone in downstream river, particularly for the solid phase. These results corroborated with the physico-chemical data obtained.

Urban catchment hydrology overwhelms reach scale effects of riparian vegetation on organic matter dynamics

1. Urbanisation severely affects stream hydrology, biotic integrity and water quality, but relatively little is known about effects on organic matter dynamics. Coarse particulate organic matter (CPOM) is a source of energy and nutrients in aquatic systems, and its availability has implications for ecosystem productivity and aquatic communities. In undisturbed environments, allochthonous inputs from riparian zones provide critical energy subsidies, but the extent to which this occurs in urbanised streams is poorly understood. 2. We investigated CPOM inputs, standing stocks, retention rates and retention mechanisms in urban and peri-urban streams in Melbourne, Australia. Six streams were chosen along a gradient of catchment urbanisation, with the presence of reach scale riparian canopy cover as a second factor. CPOM retention was assessed at baseflow via replicate releases of marked Eucalyptus leaves where the retention distance and mechanism were recorded. CPOM and small wood (>1 cm diameter) storage were measured via cores and direct counts, respectively, while lateral and horizontal CPOM inputs were assessed using riparian litter traps. Stream discharge, velocity, depth and width were also measured. 3. CPOM inputs were not correlated with urbanisation, but were significantly higher in ‘closed’ canopy reaches. Urbanisation and riparian cover altered CPOM retention mechanisms, but not retention distances. Urban streams showed greater retention by rocks; while in less urban streams, retention by small wood was considerably higher. CPOM and small wood storage were significantly lower in more urban streams, but we found only a weak effect of riparian cover. 4. These findings suggest that while riparian vegetation increases CPOM inputs and has modest/weak effects on storage, catchment scale urbanisation decreases organic matter availability. Using an organic matter budget approach, it appears likely that the increased frequency and magnitude of high flows associated with catchment urbanisation exerts an overriding influence on organic matter availability. 5. We conclude that to maintain both organic matter inputs and storage, the restoration and protection of streams in urban or rapidly urbanising environments relies on the management of both riparian vegetation and catchment hydrology.

Natural impacted freshwaters: in situ use of alginate immobilized algae to the assessment of algal response

The objective of this study was to investigate the feasibility of an in situ phytotoxicity test using alginate-immobilized algae for 60 days, in the assessment of water quality in an impacted small peri-urban stream. After laboratory optimization of algae immobilization/de-immobilization processes, the performance of immobilized/de-immobilized algae was compared to the performance of free algae in terms of specific algal growth and sensitivity. This was done by comparing 72 h EC50 values obtained with zinc and the pesticides clomazone and carbofuran. The results showed a similar performance, which allow us to conclude that immobilization for 60 days do not cause any significant alteration in algae physiology. In the field, immobilized algae were exposed at different times (2, 4 and 7 days) to water samples in both disturbed and undisturbed sites. Both laboratory and field experiments indicated that alginate-immobilized algae for 60 days were sufficiently sensitive for use in the in situ assessment of water quality.

Tegumentary leishmaniasis outbreak in Bella Vista City, Corrientes, Argentina during 2003

Bella Vista City, Corrientes, Argentina, reported an epidemic outbreak of tegumentary leishmaniasis during 2003. The mean age of the 31 cases was 25.0 +/- 13.7 years old, with a sex ratio male:female 1.8, and without mucosal involvement. They clustered in two contiguous neighbourhoods, 96% in the periurban border and 4% in the peripheral outskirts. The transmission peak was estimated to have occurred during April 2003. Four species (3608 sand flies) were captured in nine sites: Lutzomyia neivai (90.1%), Lu. pessoai (8.9%), Lu. migonei (0.8 %), and Brumptomyia avellari (0.2 %). The outskirts/rural capture ratio of Lu. neivai was up to 3, and the outskirts/periurban up to 200. Therefore, the 'urban' transmission in this southernmost known focus is still an ecotone-border associated risk. The changes in human distribution or activities, patches of the secondary vegetation, periurban streams, rainfall of the previous year, and river period floods could all contribute to 'urban' outbreaks in the region. Tegumentary leishmaniasis risk should be assessed for any project that involves changes in land use throughout an endemic area.