Perfusion Rate Research Articles

Dynamics of Indicators of the Fetoplacental Complex during Ozone Therapy: a Prospective Comparative Study of 93 Women with Acute Serous Gestational Pyelonephritis

INTRODUCTION. The beneficial effect of the ozone therapy use in acute serous gestational pyelonephritis is well-known. However, in the scientific literature there is no information on the effect of medical ozone on the functional state of placental fetal blood flow with this pathology. AIM. To study the dynamics of fetoplacental complex parameters in women suffering from acute serous gestational pyelonephritis using ozone-oxygen mixture. MATERIAL AND METHODS. The studies were conducted on 93 pregnant women at the age of 28 (16-39) years old suffering from acute serous gestational pyelonephritis, gestational age was from 14 to 26 weeks, who were randomized into two groups: the first/study (n=46) group and the second/control (n=47) one. The control group of pregnant women took only the drug standard according to the Federal Clinical Guidelines. In the study group, drug therapy was supplemented by intravenous administration of an ozone-oxygen mixture. Both groups were evaluated in the dynamics of ultrasound examination of the fetoplacental complex, as well as uterine and placental blood flow Dopplerometry. DISCUSSION. The increase in the effectiveness of restorative treatment of women with acute serous gestational pyelonephritis including ozone therapy is due to an increase in the initially reduced diastolic blood perfusion rate; summation of the therapeutic effects of ozone and pharmacotherapy; enhancement of pharmacopotential value of the used medicinal preparation. CONCLUSION. Parenteral application of ozone-oxygen mixture in programs of restorative treatment of women with acute serous gestational pyelonephritis provides significant improvement of functional state of placental-uterine blood flow.

Tissue-engineered anterior segment eye cultures demonstrate hallmarks of conventional organ culture

BackgroundGlaucoma is a blinding disease largely caused by dysregulation of outflow through the trabecular meshwork (TM), resulting in elevated intraocular pressure (IOP). We hypothesized that transplanting TM cells into a decellularized, tissue-engineered anterior segment eye culture could restore the outflow structure and function.MethodsPorcine eyes were decellularized with freeze–thaw cycles and perfusion of surfactant. We seeded control scaffolds with CrFK cells transduced with lentiviral vectors to stably express eGFP and compared them to scaffolds seeded with primary TM cells as well as to normal, unaltered eyes. We tracked the repopulation behavior, performed IOP maintenance challenges, and analyzed the histology.ResultsTransplanted cells localized to the TM and progressively infiltrated the extracellular matrix, reaching a distribution comparable to normal, unaltered eyes. After a perfusion rate challenge to mimic a glaucomatous pressure elevation, transplanted and normal eyes reestablished a normal intraocular pressure (transplanted = 16.5 ± 0.9 mmHg, normal = 16.9 ± 0.9). However, eyes reseeded with eGFP-expressing CrFK cells could not regulate IOP, remaining high and unstable (27.0 ± 6.2 mmHg) instead.ConclusionTissue-engineered anterior segment scaffolds can serve as readily available, scalable ocular perfusion cultures. This could reduce dependency on scarce donor globes in outflow research and may allow engineering perfusion cultures with specific geno- and phenotypes.

Analytical and numerical analysis of the dual-pulse lag heat transfer in a three-dimensional tissue subjected to a moving multi-point laser beam

An extensive algorithm based on both analytical and numerical solution methodologies is proposed to obtain transient temperature distributions in a three-dimensional living tissue subjected to a moving single-point and multi-point laser beam by considering metabolic heat generation and blood perfusion rate. Here, the dual-phase lag/Pennes equation is analytically solved by using the method of Fourier series and the Laplace transform. The ability to model single-point or multi-point laser beams as an arbitrary function of place and time is a significant advantage of the proposed analytical approach, which can be used to solve similar heat transfer problems in other living tissues. Besides, the related heat conduction problem is numerically solved based on the finite element method. The effects of laser beam transitional speed, laser power, and the number of laser points on the temperature distribution within the skin tissue are investigated. Moreover, the temperature distribution predicted by the dual-phase lag model is compared with that of the Pennes model under different working conditions. For the studied cases, it is observed that the maximum tissue temperature decreased about 63% by an increase of 6mm/s in the speed of the laser beam. An increase in the laser power from 0.8W/cm3 to 1.2W/cm3 results in a 28 °C increase in the maximum temperature of the skin tissue. It is observed that the maximum temperature predicted by the dual-phase lag model is always lower than that of the Pennes model and the temperature variations over time are sharper, while their results are entirely consistent over the simulation time. The obtained numerical results indicated that the dual-phase lag model is preferred in heating processes occurring at short intervals. Among the investigated parameters, the laser beam speed has the most considerable effect on the difference between the results of the Pennes and the dual-phase lag models.

Perfusion process for CHO cell producing monoclonal antibody: Comparison of methods to determine optimum cell specific perfusion rate

Over twenty-five years ago, perfusion was applied to mammalian cells to produce therapeutic proteins, mainly sensitive ones. Nowadays, other issues such as process intensification make perfusion attractive again. However, it is a complex process to set up compared to the classical fed-batch approach. In this paper, we compared three culture methods to determine the perfusion process parameters for a CHO cell line producing a monoclonal antibody. The push-to-low (PTL) and perfusion-without-bleeding (PWB) methods were performed in bioreactors equipped with a cell retention device, while the semi-perfusion (SP) method was performed in spin tubes. In our culture conditions, the PTL reference method, performed at steady state, indicated a minimum cell specific perfusion rate (CSPRmin) estimated at 100 pL.cell−1.day−1. The PWB and SP methods led to lower CSPRmin estimations related to lower cell metabolic fluxes. We therefore propose a three-step approach to determine the perfusion parameters: (i) SP screening method to estimate the optimum medium and temperature, then (ii) PWB run to determine both the growth rate and the maximum cell concentration and finally, (iii) the perfusion process can be operated according to the previously determined parameters, with a final adjustment of the CSPRmin.

Effect of Co-culture of mesenchymal stem cell and glomerulus endothelial cell to promote endothelialization under optimized perfusion flow rate in whole renal ECM scaffold.

In recent era, many researches on implantable bio-artificial organs has been increased owing to large gap between donors and receivers. Comprehensive organ based researches on perfusion culture for cell injury using different flow rate have not been conducted at the cellular level. The present study investigated the co-culture of rat glomerulus endothelial cell (rGEC) and rat bone marrow mesenchymal stem cells (rBMSC) to develop micro vascularization in the kidney scaffolds culturing by bioreactor system. To obtain kidney scaffold, extracted rat kidneys were decellularized by 1% sodium dodecyl sulfate (SDS), 1% triton X-100, and distilled water. Expanded rGECs were injected through decellularized kidney scaffold artery and cultured using bioreactor system. Vascular endothelial cells adhered and proliferated on the renal ECM scaffold in the bioreactor system for 3, 7 and 14 days. Static, 1​ml/min and 2​ml/min flow rates (FR) were tested and among them, 1​ml/min flow rate was selected based on cell viability, glomerulus character, inflammation/endothelialization proteins expression level. However, the flow injury was still existed on primary cell cultured at vessel in kidney scaffold. Therefore, co-culture of rGEC​+​rBMSC found suitable to possibly solve this problem and resulted increased cell proliferation and micro-vascularization in the glomerulus, reducing inflammation and cell death which induced by flow injury. The optimized perfusion rate under rGEC​+​rBMSC co-culture conditions resulted in enhanced endocellularization to make ECM derived implantable renal scaffold and might be useful as a way of treatment of the acute renal failure.

Designing Polymer-based Mucosa Membranes: Biomimicking

Mucoadhesion develops when a polymer adheres to the mucosal membrane through chemical or physical interactions. Adhesive materials are often employed in the manufacture of dosage forms for transmucosal drug administration via oral, nasal, esophageal, buccal and vaginal routes. This review covers some of the most prevalent synthetic methods for improving the mucoadhesive characteristics of polymeric materials. The buccal film and the oral dose form are two of these administration methods. Medication with a high blood perfusion rate can easily pass through the mucosal barrier of the mouth (oral mucosa). Drugs with low bioavailability and short half-life are simpler to administer. Buccal films, as opposed to traditional drug delivery systems, enable for the regulated and prolonged release of topical pharmaceuticals and are favoured over alternative approaches for delivering medications that are at risk of being lost because to the first pass effect, reduced permeability, enzyme degradation along with the gastrointestinal system's changing environment. Superior mucoadhesive qualities are found in hydrophilic polymers that have charged groups and/or non-ionic functional groups that can establish hydrogen bonds with mucosal surfaces. There are multiple ways for assessing the mucoadhesive characteristics of different dosage formulations. This review provides an overview of the properties of mucoadhesive and mucus gel, as well as the most commonly used procedures.

Mathematical modelling and simulation of three phase lag bio-heat transfer model during cancer treatment

We have developed a two-dimensional mathematical model that described the study of bio-heat transfer. Our model is an initial boundary value problem of partial differential equation. The solution consists of the three step procedure- (i) transformation of problem in dimensionless form (ii) by using finite differences, the problem converted into ordinary matrix differential equation (iii) applying Legendre wavelet Galerkin method, the problem is transferred into the generalised system of Sylvester equations which are solved by applying Bartels-Stewart Algorithm of generalised inverse. We have used this method to determine the temperature profile in three different boundary conditions. The consequence of boundary conditions on temperature profile are discussed in detail. The effect of phase lag due to heat flux, phase lag due to temperature gradient and phase lag due to thermal displacement have been observed. And, we have seen that temperature profile increases when the phase lags decreases. We have also observed the effect of blood perfusion rate and metabolic heat generation in specific. Results are validated with exact results in particular case.

Development of recombinant adenovirus vector herpes zoster vaccine production process using perfusion strategy with tangential flow filtration system

AbstractBACKGROUNDHerpes zoster (HZ) causes painful skin and nerve lesions, and its most frequent complication is postherpetic neuralgia. Currently, vaccination is recommended to decrease the incidence of HZ, and cost‐effective vaccine production strategies are required to produce low‐cost vaccines. Perfusion cultivation is now a common trend for increasing bioreactor productivity and overcoming nutritional limitations. The tangential flow filtration (TFF) system has proven to be suitable for perfusion cultures of mammalian cells. However, perfusion cultivation exponentially increases medium consumption, and a proper perfusion strategy should be developed to supply nutrition and save medium at the same time.RESULTSIn this study, an HEK 293 cell‐based recombinant adenovirus vector herpes zoster (rAdV‐HZ) vaccine production process was developed using a novel TFF perfusion system. First, the perfusion start time, perfusion rate, and glutamine concentration were investigated in shake flasks to increase the cell density with an economical perfusion strategy. Then, the key process parameters (cell concentration at infection, multiplicity of infection, and virus production media) of the rAdV‐HZ production process were optimized using the design of experiments approach, and the robust setpoint and design space of the key process parameters were obtained. Finally, the process parameters at the robust setpoint were successfully scaled up to a 7 L bioreactor with a novel TFF system, yielding an rAdV‐HZ titer of 1.7 × 1010 infectious units mL−1.CONCLUSIONThis study can serve as a baseline for developing an economical perfusion strategy for the large‐scale production of recombinant adenovirus vector‐based vaccines. © 2022 Society of Chemical Industry (SCI).

Influence of aging and body location on the thermal performance of firefighter's clothing exposed to radiant heat source

This paper explains the effect of three different radiant heat exposures (10 kW/m2, 25 kW/m2 and 35 kW/m2) on the skin of different age-grouped fire-fighters at their different body location. A multi-layer fire-retardant garment (outer-shell, moisture barrier, and thermal-liner), an air-gap between fabric and skin, and the human-skin itself are considered in the present numerical investigation. Pennes bio heat transfer model which includes varied skin thickness, blood perfusion rate, metabolism rate, and other thermo physical properties of the skin based on their age and body-locations have been included in this study. Skin temperature obtained from the present numerical model is validated with the experimental result. The influence of different age and body segments of the firefighters on the temperature distribution as well as the thermal damage in the tissue is analyzed. The results demonstrate that the age of the firefighters adversely affects the performance of the protective gear since the protection time is comparatively alleviated for elderly firefighters. Source temperature significantly affects the burn times. For firefighters in the middle and older age groups, the time required for a first-degree burn is reduced by up to 68.89% and 73.59%, respectively, when the source temperature is increased from 780 K to 980 K. Torso is the least affected body segment at high heat exposure followed by legs and arms. The outcomes of this study can be used to create firefighter protection gear that better shields different body parts and firemen of different ages.

Pulmonary Oxygen Exchange in a Rhythmically Expanding–Contracting Alveolus–Capillary Model

Pulmonary gas exchanges are vital to human health, and disruptions to this process have been associated with many respiratory diseases. Previous gas exchange studies have predominately relied on whole-body testing and theoretical analysis with 1D or static models. However, pulmonary gas exchanges are inherently a dynamic process in 3D spaces with instantaneous interactions between air, blood, and tissue. This study aimed to develop a computational model for oxygen exchange that considered all factors mentioned above. Therefore, an integrated alveolus–membrane–capillary geometry was developed with prescribed rhythmic expansion/contraction. Airflow ventilation, blood perfusion, and oxygen diffusion were simulated using COMSOL. The temporal and spatial distribution of blood flow and oxygen within the capillaries were simulated under varying breathing depths and cardiac outputs. The results showed highly nonuniform blood flow distributions in the capillary network, while the rhythmic oscillation further increased this nonuniformity, leading to stagnant blood flow in the distal vessels. A static alveolus–capillary geometry underestimated perfusion by 11% for normal respirations, and the deviation grew with breathing depth. The rhythmic motion caused a phase lag in the blood flow. The blood PO2 reached equilibrium with the alveolar air after traveling 1/5–1/3 of the capillary network. The time to reach this equilibrium was significantly influenced by the air–blood barrier diffusivity, while it was only slightly affected by the perfusion rate. The computational platform in this study could be instrumental in obtaining refined knowledge of pulmonary O2 exchanges.

Numerical analysis of thermal response on a non-linear model of multi-layer skin under heating and cooling processes

Based on the non-linear model of multi-layer skin, the present study is performed under heating and cooling processes with linear and non-linear generalized boundary conditions. The numerical outcome is obtained utilizing Runge Kutta (4,5) along with the finite difference scheme and the accuracy of this scheme is shown graphically by comparing it with an accurate analytical outcome in a special case. When the value of γ increases, the skin temperature is gradually higher in the case of a linear boundary condition and gradually lower in the case of a non-linear boundary condition with respect to linear boundary condition at epidermis-dermis (ED) interface. During cooling, the heat effect has slightly quick vanished in a non-linear boundary condition than in a linear boundary condition. The effect of a non-linear boundary condition gradually decreases as the value of heat transfer coefficient rises, then it reflecting the nature of the first kind of linear boundary condition. The exponential blood perfusion rate has a slightly quick vanishes of heat effect in comparison to constant and linear blood perfusion rate. To investigate the conduct of the temperature distribution in multi-layer skin, all the impacts are depicted graphically.

Automated thermal imaging monitors the local response to cervical cancer brachytherapy.

Malignant tumors have high metabolic and perfusion rates, which result in a unique temperature distribution as compared to healthy tissues. Here, we sought to characterize the thermal response of the cervix following brachytherapy in women with advanced cervical carcinoma. Six patients underwent imaging with a thermal camera before a brachytherapy treatment session and after a 7-day follow-up period. A designated algorithm was used to calculate and store the texture parameters of the examined tissues across all time points. We used supervised machine learning classification methods (K Nearest Neighbors and Support Vector Machine) and unsupervised machine learning classification (K-means). Our algorithms demonstrated a 100% detection rate for physiological changes in cervical tumors before and after brachytherapy. Thus, we showed that thermal imaging combined with advanced feature extraction could potentially be used to detect tissue-specific changes in the cervix in response to local brachytherapy for cervical cancer.

A Computational Model for Temperature Monitoring During Human Liver Treatment by Nd:YaG Laser Interstitial Thermal Therapy (LITT)

Describing heat transfer in biological organs is absolutely challenging because it is involved with many complex phenomena. Therefore, understanding the optical and thermal properties of living system during external irradiation sources such as laser interstitial thermal therapy (LITT) are too important for therapeutic purposes, especially for hyperthermia treatments. The purpose of this study was to determine a proper laser power and irradiation time for LITT applicator to irradiate liver tissue during hyperthermia treatment. For this aim, bioheat equation in one-dimensional spherical coordinate is solved by Green function method to simulate temperature distribution and rate of damage around irradiated target and how thermal and optical properties such as laser power, laser exposure time, and blood perfusion rate affect the rate of temperature distribution. Guiding equations according to the suggested boundary conditions are written and solved by MATLAB software. The outcomes show that increasing laser exposure time and power increase the temperature, especially at the nearest distance from the center of diffusion. Accordingly, a decrease in blood perfusion rate leads to decrease temperature distribution. The findings show that the model is useful to help the physicians to monitor the amount of heat diffusion by laser power during the treatment to protect healthy cells.

Why Do Antibiotics Fail? A Veterinary Perspective

In both human and veterinary medicine, the failure of apparently appropriate antimicrobial therapy is a common and often exasperating clinical problem. Many factors are associated with the failure of antibiotic therapy, including an incorrect diagnosis of infectious disease, selection of the inappropriate antibiotic, and incorrect dosage. To achieve the best results, bacteriological diagnostics in the laboratory should be made. Even though the in-vitro antimicrobial susceptibility testing guides the potentially suitable antimicrobials, the in-vitro susceptibility obtained is not always the best in-vivo. The clinician should be aware of other factors, including biofilm-forming bacteria, physicochemical conditions at the site of infection (such as perfusion rate, oxygen partial pressure, and pH value), or immunosuppression of the patient that can lead to failure of the treatment. This review summarized the main factors associated with antibiotic failure in a veterinarian practice. In a world where animal and human resistance to an antibiotic is rising every year, rational and efficient use of antibiotic therapy is of utmost importance. It is essential to continue with the education of veterinary practitioners in all aspects of antimicrobial resistance and treatment to improve future treatments and have a more rational use of antibiotics to reduce antibiotic resistance in animals and humans.

Soluble epoxide hydrolase inhibitor, t-AUCB, improves salivary gland function by ameliorating endothelial injury

AimsInhibitor of soluble epoxide hydrolase (t-AUCB) has been used in the experimental therapy of hypertension. This study aimed to investigate whether the secretion of submandibular glands (SMGs) altered in renal hypertensive rats, and to explore whether t-AUCB could improve the salivary secretion. Main methods2-kidney 1-clip Sprague-Dawley rats were used as renal hypertensive animals. t-AUCB treatment was given for 1 week after 8 weeks modeling. Blood pressure, blood perfusion and the secretion of SMGs, and endothelium-dependent relaxation of external maxillary artery were measured to investigate the effects of t-AUCB on the vascular tone and the secretion of SMGs in renal hypertensive rats. SMGs were collected for histological evaluation and the internal arteries were dissected for primary endothelial cells culture. Key findingsThe blood perfusion and flow rate of SMGs in the renal hypertensive rats were significantly lower than those in the controls. Endothelium-dependent relaxation of the external maxillary artery and AMPK/Akt/eNOS signaling was impaired in hypertensive rats. The glandular morphology and the concentration of salivary ions did not change obviously. t-AUCB treatment ameliorated the secretion of SMGs, the blood perfusion, and the dysfunction of endothelium-dependent relaxation of the external maxillary artery by activating the AMPK/Akt/eNOS pathway in hypertensive rats. Significancet-AUCB increases the blood perfusion through ameliorating dysfunction of endothelium-dependent relaxation of SMGs arteries and thus improves the hyposecretion of SMGs in hypertensive rats.

Efficacy of Human Embryonic Stem Cells Compared to Adipose Tissue-Derived Human Mesenchymal Stem/Stromal Cells for Repair of Murine Post-Stenotic Kidneys.

Clinical translation of mesenchymal stem/stromal cell (MSC) therapy has been impeded by the heterogenous nature and limited replicative potential of adult-derived MSCs. Human embryonic stem cell-derived MSCs (hESC-MSCs) that differentiate from immortal cell lines are phenotypically uniform and have shown promise in-vitro and in many disease models. Similarly, adipose tissue-derived MSCs (MSC(AT)) possess potent reparative properties. How these two cell types compare in efficacy, however, remains unknown. We randomly assigned mice to six groups (n = 7-8 each) that underwent unilateral RAS or a sham procedure (3 groups each). Two weeks post-operation, each mouse was administered either vehicle, MSC(AT)s, or hESC-MSCs (5 × 105 cells) into the aorta. Mice were scanned with micro-MRI to determine renal hemodynamics two weeks later and kidneys then harvested. hESC-MSCs and MSC(AT)s were similarly effective at lowering systolic blood pressure. However, MSC(AT)s more robustly increased renal perfusion, oxygenation, and glomerular filtration rate in the post-stenotic kidney, and more effectively mitigated tubular injury, fibrosis, and vascular remodeling. These observations suggest that MSC(AT) are more effective than hESC-MSC in ameliorating kidney dysfunction and tissue injury distal to RAS. Our findings highlight the importance of tissue source in selection of MSCs for therapeutic purposes and underscore the utility of cell-based therapy for kidney disease.

Numerical simulation of the skin tissue subjected to hyperthermia treatment using a nonlinear DPL model

This article deals with the study of simulation-based mathematical modeling of tissue using the non-linear dual-phase-lag bioheat transfer (DPLBHT) model for hyperthermia treatment of tumor or cancer cells with Gaussian distribution type heat source. The metabolic heat source as an exponential variation, blood perfusion rate as a linear variation and thermal conductivity as a linear variation are considered to be temperature-dependent, resulted in a more accurate prediction of the non-linear DPLBHT model. At initial time, τq is more effective than τt in the targeted region. When the infected or cancerous cells increases in width then for effective treatment the value of the antenna constant could be decreased and the value of probe region can be changed according to the tumor location in the skin tissue. When the value of γ, Wbo, a1 and a2 increases, the temperature profile in the target region decreases. The numerical solution is obtained by hybrid scheme using Runge Kutta (4,5) and finite difference technique. This technique is compared for accuracy with an exact analytical result in a particular case as shown in figures and table and found in good compliance. The effect of lagging, metabolic heat source, blood perfusion rate, coefficient of thermal conductivity, external heat source parameter and the other associated parameter are studied in detail and the results are presented graphically.

Metformin-induced alterations in gills of the freshwater fish Astyanax lacustris (Lütken, 1875) detected by histological and scanning electron microscopy.

The antidiabetic drug metformin is widely prescribed and found in different concentrations in the environment around the world, raising concern about potential impacts on aquatic life. Analyses of the effects of exposure of biological models to aquatic contaminants are important for assessing pollution effects on fish health. The gills of fishes represent primary targets of disturbance by pollutants, mainly because of the large surface of the respiratory epithelium and the high perfusion rate, which both help the entry of pollutants into this tissue. In this context, the aim of this work was to use gill histological analyses biomarkers to evaluate the toxicity of metformin on aquatic environmental systems, by means of chronic exposure for 90 days of Astyanax lacustris (lambari), an ecologically important neotropical species that can be used as an environmental bioindicator. Histopathological analyses were performed using Light and Scanning Electron Microscopy. The main changes were lamellar fusion, telangiectasia hyperplasia and disappearance of microridges. The morphological changes observed possibly interfere with the gill physiology, indicating an unfavorable situation to the presence of metformin in the water, pointing to a concern that metformin may pose a risk to Astyanax lacustris and likely to other fish species, compromising the dynamics of the aquatic ecosystem as a whole. Graphical abstract.

Targeting pulmonary vascular endothelial cells for the treatment of respiratory diseases.

Pulmonary vascular endothelial cells (VECs) are the main damaged cells in the pathogenesis of various respiratory diseases and they mediate the development and regulation of the diseases. Effective intervention targeting pulmonary VECs is of great significance for the treatment of respiratory diseases. A variety of cell markers are expressed on the surface of VECs, some of which can be specifically combined with the drugs or carriers modified by corresponding ligands such as ICAM-1, PECAM-1, and P-selectin, to achieve effective delivery of drugs in lung tissues. In addition, the great endothelial surface area of the pulmonary vessels, the “first pass effect” of venous blood in lung tissues, and the high volume and relatively slow blood perfusion rate of pulmonary capillaries further promote the drug distribution in lung tissues. This review summarizes the representative markers at the onset of respiratory diseases, drug delivery systems designed to target these markers and their therapeutic effects.

Procedural Efficiency, Efficacy, and Safety of High-Power, Short-Duration Radiofrequency Ablation Delivered by STSF Catheter for Paroxysmal Atrial Fibrillation.

Objectives To investigate the procedural efficiency, efficacy, and safety of high-power, short-term radiofrequency ablation delivered by the SmartTouch Surround Flow (STSF) catheter for paroxysmal atrial fibrillation (AF). Methods We retrospectively analyzed a total of 72 patients who were admitted with paroxysmal AF, and who underwent radiofrequency catheter ablation (RFCA) for the first time. Of these patients, 36 cases underwent low-power, long-duration (LPLD, (30–35 W/20–40 s) pulmonary vein isolation (PVI) delivered by an SmartTouch (ST) catheter (control group), and the other 36 cases underwent high-power, short-duration (HPSD, (45–50 W/10–20 s) PVI delivered by a STSF catheter (study group). The baseline data, duration of PVI, procedural time, fluoroscopy time, the rate of first-pass isolation, irrigation perfusion, eschar and steam pop occurrences, intraoperative complications, and the rate of stable sinus rhythm maintenance following a blanking period of three months were analyzed between the two groups. Results The isolation time of bilateral PVI and procedural time in the study group were markedly less than in controls (p < 0.01). The rate of first-pass isolation in the study group was significantly higher than in the control group (95.8% vs. 84.7%, p = 0.023), while the fluid perfusion in the study group was approximately 20% less than that in the control group (767 ± 171 vs. 966 ± 227 ml, p < 0.001). We observed no severe complications in any patients. The rate of freedom from AF recurrences following a blanking period of three months showed a tendency to be higher than in controls (93.9% vs. 87.1%, p = 0.348). Conclusions The HPSD strategy delivered by the STSF catheter was superior to conventional LPLD ablation through the ST catheter with respect to efficiency, acute procedural effectiveness, short-term safety, and the risk of heart failure in patients with paroxysmal AF.