Scent Production Research Articles

Pollination‐related plant traits under environmental changes: Seasonal and daily mismatches produce temporal constraints

Abstract Pollination is a key tenet of ecosystem sustainability and food security, but it is threatened by climate change. While many studies investigated the response of plant‐pollination traits to temperature, few attempted multifactorial and integrative approaches with multiple floral traits. We determined which plant‐pollination traits were disrupted by environmental changes and the consequence for plant fitness. We focused on the thermogenic plant, Arum italicum, which relies on floral scent and heat production to attract its pollinators with a deceptive strategy. In a field mesocosm, we measured the response of floral phenology, thermogenesis traits, scent and plant fitness to experimental changes in shade level, water supply and soil temperature. In the laboratory, we exposed plants to extreme heat to determine the thermoregulation ability at high temperatures. Plant fitness decreased dramatically in response to soil warming and the level of shade. While both factors caused a lower number of inflorescences, soil warming was mostly responsible for the crash in number of fruits. The plant traits related to thermogenesis and scent were relatively resilient to the environmental changes in the mesocosm and were unlikely to produce the observed decrease in plant fitness. First, environmental changes only weakly modified the scent composition. Second, the appendix regulated its maximal temperature almost perfectly, while stamen regulated imperfectly. In the laboratory experiment, the appendix maintained a thermal gradient supposedly to improve scent emission while stamen became cooler than ambient air, possibly preventing the pollinators from overheating within the floral chamber. By contrast, the phenology of flowering was drastically modified. Flowers appeared earlier in the season in response to soil warming (by 39 days) or absence of shade (by 19 days). At the daily scale, flowering hour did not change across the season resulting in plants flowering before sunset late in the season and before the pollinator activity window (mostly nocturnal). The strong shift observed in the flowering period may induce a temporal mismatch with this pollinator both early and late in the season when the insects may not be active so early in the spring and during daytime, respectively, thereby altering plant fitness. Read the free Plain Language Summary for this article on the Journal blog.

Multi-omics analyzes of Rosa gigantea illuminate tea scent biosynthesis and release mechanisms.

Rose is an important ornamental crop cultivated globally for perfume production. However, our understanding of the mechanisms underlying scent production and molecular breeding for fragrance is hindered by the lack of a reference genome for tea roses. We present the first complete telomere-to-telomere (T2T) genome of Rosa gigantea, with high quality (QV > 60), including detailed characterization of the structural features of repetitive regions. The expansion of genes associated with phenylpropanoid biosynthesis may account for the unique tea scent. We uncover the release rhythm of aromatic volatile organic compounds and their gene regulatory networks through comparative genomics and time-ordered gene co-expression networks. Analyzes of eugenol homologs demonstrate how plants attract pollinators using specialized phenylpropanoids in specific tissues. This study highlights the conservation and utilization of genetic diversity from wild endangered species through multi-omics approaches, providing a scientific foundation for enhancing rose fragrance via de novo domestication.

Constructing Highly Efficient Catalysts for the 1‐Butene Epoxidation

AbstractEpoxides are high‐valued intermediates in the production of chemicals, pharmaceuticals, perfumes, and polymers. Given the growing demand for epoxides, it is imperative to develop more environmental friendly and sustainable routes instead of the chlorohydrin process. Notably, the direct utilization of hydrogen peroxide (H2O2) for the epoxidation reaction presents significant advantages from both environmental and economic perspectives. The review provides insights into both homogeneous and heterogeneous catalysts employed in the 1‐butene epoxidation using the green oxidant H2O2. Among the diverse range of catalysts, titanosilicate‐1 (TS‐1) has garnered extensive attention due to its exceptional selectivity and high oxygen atom utilization. The aim of this review is to illustrate various strategies for TS‐1 catalysts preparation that can lead to more versatile, higher‐performance, and greener epoxidation processes. Additionally, various potential approaches to enhance the catalyst performance of TS‐1 are highlighted, including (i) constructing specific coordination modes of Ti sites, (ii) regulating the microenvironment around Ti sites, and (iii) improving the accessibility of Ti sites. Furthermore, advances in molding TS‐1 catalysts are also introduced from the perspective of the industrialization. Finally, future research directions are discussed with emphasis on the application scope of TS‐1 to gain deeper insights into epoxidation process.

Preparation, characterization and heat release behavior of inclusion complexes formed between carvone or limonene and acyclic cucurbit[n]urils.

Carvone and limonene are naturally occurring monoterpenoids with unique aromas, making them valuable substances in synthetic fragrance production. However, their application is limited due to low stability and rapid volatilization. To address this challenge, host-guest complexes offer a promising solution. In this study, two acyclic cucurbit[n]urils were synthesized to form inclusion complexes with carvone and limonene, aiming to enhance their thermal stability and achieve excellent heat release properties. The binding behavior of the complexes was investigated using NMR, X-ray diffraction (XRD), Fourier transform infrared (FTIR) and molecular bonding analyses, confirming the formation of host-guest inclusion complexes. Our study successfully prepared four inclusion complexes (M1/CA, M2/CA, M1/LI, M2/LI) and characterized them using NMR, XRD and FTIR techniques. These complexes exhibited a 1:1 stoichiometric ratio, and their binding constants were determined through fluorescence titration. The thermal controlled release experiment shows that the degree of carvone and limonene release is different with a change of temperature, indicating that the inclusion complexes have good thermally controlled release performance, and the thermal release retention rate has a certain correlation with KS value. The larger the KS value, the higher the thermal release retention rate of the inclusion complexes, the lower the volatilization of the inclusion complexes, the longer the retention time and the better the thermal stability. This study presents a novel approach for developing carvone- and limonene-based fragrances, expanding their application potential in various industries. © 2024 Society of Chemical Industry.

Investigation of genes involved in scent and color production in Rosa damascena Mill

Rosa damascena Mill., commonly known as the King Flower, is a fragrant and important species of the Rosaceae family. It is widely used in the perfumery and pharmaceutical industries. The scent and color of the flowers are significant characteristics of this ornamental plant. This study aimed to investigate the relative expression of MYB1, CCD1, FLS, PAL, CER1, GT1, ANS and PAR genes under two growth stages (S1 and S2) in two morphs. The CCD1 gene pathway is highly correlated with the biosynthesis of volatile compounds. The results showed that the overexpression of MYB1, one of the important transcription factors in the production of fragrance and color, in the Hot pink morph of sample S2 increased the expression of PAR, PAL, FLS, RhGT1, CCD1, ANS, CER1, and GGPPS. The methyl jasmonate (MeJA) stimulant had a positive and cumulative effect on gene expression in most genes, such as FLS in ACC.26 of the S2 sample, RhGT1, MYB1, CCD1, PAR, ANS, CER1, and PAL in ACC.1. To further study, a comprehensive analysis was performed to evaluate the relationship between the principal volatile compounds and colors. Our data suggest that the rose with pink flowers had a higher accumulation content of flavonoids and anthocyanin. To separate essential oil compounds, GC/MS analysis identified 26 compounds in four samples. The highest amount of geraniol, one of the main components of damask rose, was found in the Hot pink flower, 23.54%, under the influence of the MeJA hormone.

Perancangan Kebijakan Persediaan e-Commerce dengan Pertimbangan Permintaan Probabilistik dan Performance Score

Julis, a local company focused on the production and sale of perfumes through e-commerce platforms, is one of the SMEs facing such challenges. Julis has encountered difficulties in managing its product inventory, leading to high inventory costs and low customer order fulfillment rates (fill rate). These issues risk hindering the company's growth and diminishing consumer trust. This research aims to design optimal inventory policies for Julis by considering probabilistic demand and the company's performance score. This performance score is a measure of performance that integrates two key criteria: profit, calculated based on total inventory costs, and fill rate. With the right inventory policies, Julis is expected to improve inventory efficiency, reduce costs—particularly ensuring inventory costs meet the target of less than 40% of the company's total revenue or income—and achieve a minimum fill rate target of 95%, which is considered crucial for maintaining customer satisfaction and supporting business growth.The approach used in this research is an inventory optimization model that combines probabilistic methods in determining Order Quantity (Q) and Reorder Point (ROP). This design aims to address demand uncertainty, which often poses a major challenge in inventory management within the e-commerce sector. Additionally, this research introduces the integration of inventory classification based on performance score, enabling the company to prioritize certain products based on relevant performance criteria. As a result, each product category can be managed more efficiently and strategically according to business priorities. The results of this research indicate that the implementation of the designed inventory policies can significantly improve Julis' performance score. Specifically, in terms of cost efficiency, these policies can reduce inventory costs and increasing the fill rate, which directly positively impacts the company's profitability. Furthermore, the integration of inventory classification based on performance score has also proven effective in helping Julis identify the most critical products to prioritize in its inventory management.

INOVASI GAMIFIKASI WEBSITE PERSY PERFUME SEBAGAI PENGALAMAN KONSUMEN DALAM PEMILIHAN PARFUM KEPRIBADIAN SECARA DIGITAL

This research will discuss how the gamification innovation of the Persy Perfume website in providing a consumer experience for digital personality perfume selection. The trend of using perfume products in Indonesia is increasing, marked by the emergence of many local perfume brands with globally competitive quality. However, product innovation is needed to increase consumer interest in using the product, one of the strategies that can be used is to provide a consumer experience in purchasing products. Persy Perfume is a local brand that uses the aroma of Indonesian spices as a unique selling product and elevates the local culture of wayang as a variant of each scent. Persy through its website provides a consumer experience through "Petualangan Persy Aroma" as a form of innovation in the form of personality test gamification to provide perfume scent recommendations that match the consumer's personality in digital purchases. This research is research with a qualitative descriptive method, to find out how gamification innovations in providing consumer experience to increase consumer interest in shopping for perfume digitally. The result found is that Persy Perfume has found a new innovation in providing a consumer experience through the " Petualangan Persy Aroma", this is an interesting innovation and a new way in the perfume industry to provide a digital consumer experience. By providing a new experience for consumers, this can invite interest from potential consumers to buy the product.

Autonomic Responses Associated with Olfactory Preferences of Fragrance Consumers: Skin Conductance, Respiration, and Heart Rate.

Assessing the olfactory preferences of consumers is an important aspect of fragrance product development and marketing. With the advancement of wearable device technologies, physiological signals hold great potential for evaluating olfactory preferences. However, there is currently a lack of relevant studies and specific explanatory procedures for preference assessment methods that are based on physiological signals. In response to this gap, a synchronous data acquisition system was established using the ErgoLAB multi-channel physiology instrument and olfactory experience tester. Thirty-three participants were recruited for the olfactory preference experiments, and three types of autonomic response data (skin conductance, respiration, and heart rate) were collected. The results of both individual and overall analyses indicated that olfactory preferences can lead to changes in skin conductance (SC), respiration (RESP), and heart rate (HR). The trends of change in both RESP and HR showed significant differences (with the HR being more easily distinguishable), while the SC did not exhibit significant differences across different olfactory perception preferences. Additionally, gender differences did not result in significant variations. Therefore, HR is more suitable for evaluating olfactory perception preferences, followed by RESP, while SC shows the least effect. Moreover, a logistic regression model with a high accuracy (84.1%) in predicting olfactory perception preferences was developed using the changes in the RESP and HR features. This study has significant implications for advancing the assessment of consumer olfactory preferences.

The Complexity of Volatile Terpene Biosynthesis in Roses: Particular Insights into β-Citronellol Production.

The fascinating scent of rose (Rosa genus) flowers has captivated human senses for centuries, making them one of the most popular and widely used floral fragrances. Despite much progress over the last decade, many biochemical pathways responsible for rose scents remain unclear. We analyzed the floral scent compositions from various rose varieties and selected the modern cultivar Rosa hybrida 'Double Delight' as a model system to unravel the formation of rose dominant volatile terpenes, which contribute substantially to the rose fragrance. Key genes involved in rose terpene biosynthesis were functionally characterized. Cytosolic geranyl diphosphate (GPP) generated by geranyl/farnesyl diphosphate synthase (G/FPPS1) catalysis, played a pivotal role in rose scent production, and terpene synthases (TPSs) in roses play an important role in the formation of most volatile terpenes, but not for geraniol, citral or β-citronellol. Subsequently, a series of enzymes, including geraniol dehydrogenase (GeDH), geranial reductase (GER), 12-oxophytodienoate reductase (OPR) and citronellal reductase (CAR), were characterized as involved in the transformation of geraniol to β-citronellol in roses through three successive steps. Interestingly, the β-citronellol biosynthesis pathway appears to be conserved in other horticultural plants like Lagerstroemia caudata and Paeonia lactiflora. Our findings provide valuable insights into the biosynthesis of rose volatile terpenoid compounds and offer essential gene resources for future breeding and molecular modification efforts.

Synthetic Endocrine Disruptors in Fragranced Products

Endocrine disruptors are molecules that can interfere with the proper functioning of the endocrine system and lead to harmful effects in living organisms. This review focuses on the impact of synthetic fragrances, which are commonly found in personal care and household products, on the endocrine system. The article discusses the different types of hormones in the body and how they interact with receptors to produce signals. It also explores how endocrine disruptors can interfere with hormone signaling and transport, leading to adverse effects in the body. This work underscores the crucial need for further research into the impact of synthetic fragrances on the endocrine system and the importance of using safer alternatives in personal care and household products.

Formulation and Evaluation of Solid Perfume based on Bees Wax

Solid perfume based on beeswax is a unique and natural fragrance product that provides a long-lasting scent while being gentle on the skin. This type of perfume is made from a combination of natural ingredients, including beeswax, carrier oils, and essential oils. The beeswax acts as a natural emulsifier, which helps to blend the other ingredients and create a solid, compact texture. The carrier oils and essential oils provide the fragrance, which is released slowly and subtly throughout the day. Solid perfume based on beeswax is an excellent alternative to traditional liquid perfumes, as it is more portable, easy to apply, and environmentally friendly. It is also a perfect choice for those with sensitive skin, it contains no alcohol or synthetic ingredients that can irritate the skin. To ensure the success of the presented collection, several quality factors have been established, which will be the same for all four perfumes in the collection, as well as the evaluation indexes of these factors. Quality factors have been classified as sensory, with factors such as the applicability of the product to the skin, and physicochemical, establishing the melting point and stability of the product.mm/second, melting point 90°C and pH value 4.

Inducing Aquilaria sinensis (Lour.) Spreng “Qinan” Agarwood Formation with Chemical Reagents

Agarwood is a highly sought-after resinous wood for use in medicine, incense, and perfume production. However, the process of agarwood formation is slow. In this study, the induction of agarwood formation in three-year-old Aquilaria sinensis “Qinan” trees through treatment with ethephon, hydrogen peroxide, sodium chloride, distilled water, and wounding was investigated. The results showed that the starch content significantly decreased and that the soluble sugar content significantly increased during the process of agarwood formation, with no significant difference between the different treatment groups. The net photosynthetic rate significantly decreased. Except for the sodium chloride treatment, the total antioxidant capacity and the contents of flavonoids and phenols significantly increased during the process of agarwood formation. The essential oil yield of the ethephon and hydrogen peroxide treatment groups was significantly higher than that of the other treatment groups, with the highest essential oil yield in the ethephon treatment group. Compared to the control group, salt treatment produced the lowest essential oil yield of agarwood. The results indicate that appropriate concentrations of ethephon and hydrogen peroxide can promote the formation of agarwood in A. sinensis “Qinan”.

Metabolome and Transcriptome Unveil the Correlated Metabolites and Transcripts with 2-acetyl-1-pyrroline in Fragrant Rice.

Fragrance is a valuable trait in rice varieties, with its aroma significantly influencing consumer preference. In this study, we conducted comprehensive metabolome and transcriptome analyses to elucidate the genetic and biochemical basis of fragrance in the Shangsixiangnuo (SSXN) variety, a fragrant indica rice cultivated in Guangxi, China. Through sensory evaluation and genetic analysis, we confirmed SSXN as strongly fragrant, with an 806 bp deletion in the BADH2 gene associated with fragrance production. In the metabolome analysis, a total of 238, 233, 105 and 60 metabolic compounds exhibited significant changes at the seedling (S), reproductive (R), filling (F), and maturation (M) stages, respectively. We identified four compounds that exhibited significant changes in SSXN across all four development stages. Our analyses revealed a significant upregulation of 2-acetyl-1-pyrroline (2AP), the well-studied aromatic compound, in SSXN compared to the non-fragrant variety. Additionally, correlation analysis identified several metabolites strongly associated with 2AP, including ethanone, 1-(1H-pyrrol-2-yl)-, 1H-pyrrole, and pyrrole. Furthermore, Weighted Gene Co-expression Network Analysis (WGCNA) analysis highlighted the magenta and yellow modules as particularly enriched in aroma-related metabolites, providing insights into the complex aromatic compounds underlying the fragrance of rice. In the transcriptome analysis, a total of 5582, 5506, 4965, and 4599 differential expressed genes (DEGs) were identified across the four developmental stages, with a notable enrichment of the common pathway amino sugar and nucleotide sugar metabolism in all stages. In our correlation analysis between metabolome and transcriptome data, the top three connected metabolites, phenol-, 3-amino-, and 2AP, along with ethanone, 1-(1H-pyrrol-2-yl)-, exhibited strong associations with transcripts, highlighting their potential roles in fragrance biosynthesis. Additionally, the downregulated expression of the P4H4 gene, encoding a procollagen-proline dioxygenase that specifically targets proline, in SSXN suggests its involvement in proline metabolism and potentially in aroma formation pathways. Overall, our study provides comprehensive insights into the genetic and biochemical mechanisms underlying fragrance production in rice, laying the foundation for further research aimed at enhancing fragrance quality in rice breeding programs.

Fabrication of bio-based polymer microcapsule containing fragrances via interfacial photo-initiated crosslinking

Home fragrance (F) products such as laundry detergent and fabric softener use fragrance or essential oils as key components. Typically, the fragrance is enclosed within a polymer. The shell enhances the longevity of the scent due to its durability. However, there is growing environmental concern about utilizing fossil polymers, which decompose slowly and harm the ecology and food chain. Biopolymers are also highly expected to be used as alternative polymers to prepare environmentally friendly microcapsules. This research aims to use poly cinnamyl methacrylate (PCMA) as a biopolymer to prepare microcapsules encapsulated fragrance via interfacial photo-initiated crosslinking. The CMA monomer was prepared by the esterification of cinnamyl alcohol with methacrylic anhydride and synthesized via radical polymerization to form PCMA with a molecular weight of 6,400 g/mol. The PCMA and PCMA/F particles were obtained when the PCMA and PCMA/F droplets dispersed in water were UV-irradiated at 60 W for 8h, giving PCMA and PCMA/F particles contained the highest crosslinking (60–75%). Both PCMA and PCMA/F particles were spherical and smooth outer surfaces where their particle sizes of ∼2 and 7 µm for PCMA and PCMA/F particles were obtained, respectively. The larger size of PCMA/F particles was due to encapsulation, where the encapsulation efficiency of fragrance in PCMA/F particles was approximately 100%. Moreover, the fragrances remaining in the microcapsule particles after 40 days of storage were over 90%. This technique was simple, had high potential microencapsulation, and was environmentally friendly, so the developed microcapsules would express great potential for textiles and laundry formulations.

THE IMPACT OF GENERATION X, Y, Z, AND DEMOGRAPHICS TRAITS IN THE RELATIONSHIP BETWEEN BRAND COMPONENTS OF CONSPICUOUS CONSUMPTION AND THIRD-PARTY ENDORSEMENT

The identification of the buying habits of the X, Y and Z generations is a topic that is still being studied and understood by companies and marketing professionals. In developing countries, conspicuous consumption varies according to the different motivations that drive the purchasing behaviour of consumers. Therefore, the aim of this study is to reveal the relationship among the demographic traits of generation X, Y and Z, the brand component and third-party endorsement in conspicuous consumption towards branded perfume products. A structural model was constructed to identify the put-forward effects and hypothesis tests were developed to measure the relationships. The research was conducted among consumers of generation X, Y, and Z in Turkey who use branded perfume products and follow at least one social media influencer. The convenience sampling method was used and benefited from the SPSS for the descriptive analysis and the Smart PLS 4.0 program for the first-order Structural Equation Modeling and mediating affect analysis of the proposed research model. The results of the research found that third-party endorsement has positively and significantly impacts conspicuous consumption and brand components. The brand components have a positive and significant effect on conspicuous consumption. Although third-party endorsement had a significant impact on demographic traits of Generation X, Y, and Z conspicuous consumption; brand components had no significant effect on Generation X, and Z conspicuous consumption. Brand components have a mediating effect on Generation Y's conspicuous consumption and demographic traits of Generation Z.

Brand Awareness Mediation: Analysis of Online Customer Reviews and Influencer Marketing on Local Perfume Purchase Decisions in E-Commerce

This research aims to determine the influence of online customer reviews and influencer marketing mediated by brand awareness on purchasing decisions for local perfumes in e-commerce. Quantitative methods were used in this research with a sampling technique using a purposive sampling method. The population in this study involved Gen Z and millennials with an age range of 17-40 years who were perfume customers in the city and district of Cirebon. The sample used was 150 respondents who met the research sample criteria. Data analysis in the research was processed using structural equation modeling (SEM-PLS) using the SmartPLS 4.1.0 application. Based on the results of the analysis of validity tests, reliability tests, discriminant validity, r-square tests, and path coefficients, results were obtained which stated that there was a positive and significant influence of each exogenous variable, namely Online Customer Reviews, Influencer Marketing on purchasing decisions. Furthermore, Brand Awareness can be a positive mediator in the correlation between Online Customer Reviews and Influencer Marketing which simultaneously influences Purchase Decision variables for local perfume products in e-commerce.

ANALYSIS OF HAZARDOUS COMPOUNDS IN REFILLABLE ROLL-ON PERFUMES CIRCULATING IN GORONTALO CITY USING GC-MS

This study aims to analyze the potentially hazardous compounds in refillable roll-on perfumes. The analytical method employed was GC-MS. The results from GC-MS analysis revealed hazardous compounds including Alpha-Pinene with concentrations in samples A-E = 0.0048 ppm, 0.0035 ppm, 0.0105 ppm, 0.0038 ppm, 0.0034 ppm; Dipropylene glycol with concentrations in samples A-E = 0.0322 ppm, 0.0162 ppm, 0.0692 ppm, 0.0493 ppm, 0.0465 ppm; Limonene with concentrations in samples A-E = 0.0705 ppm, 0.0373 ppm, 0.0222 ppm, 0.1462 ppm; Linalool with concentrations in samples A-E = 0.0092 ppm, 0.0568 ppm, 0.0146 ppm, 0.1298 ppm, 0.1233 ppm; Linalyl Acetate with concentrations in samples A-E = 0.1207 ppm, 0.0719 ppm, 0.0413 ppm, 0.1418 ppm, 0.1285 ppm. The levels of these compounds in the samples are below exposure thresholds, suggesting their current use is considered safe. However, stringent government oversight is necessary for refillable perfume products available in Gorontalo City.

Air Pollution and Ethical Consumption Behavior Due to Fragrances

This paper presents a comprehensive review of literature focusing on the detrimental effects of fragrances on air quality. While previous studies traditionally attributed air pollution to sources like automobile emissions or industrial discharge, this study delves into the impact of consumer products, particularly fragrances, on both indoor and outdoor air pollution. Through a systematic review of existing research utilizing databases such as Web of Science, Google Scholar, and Scopus, the study synthesizes findings regarding the composition of fragrances, regulatory practices related to ingredient labeling, and public awareness regarding the link between air pollution and fragrances. The findings indicate that the ingredients used in perfumes can contribute to air pollution and respiratory ailments, underscoring the need for stringent regulations. Despite this, there is a lack of comprehensive labeling requirements for perfume products worldwide, which may contribute to limited consumer awareness regarding the association between air quality and fragrances. Given the intimate relationship between air pollution and human well-being, this study underscores the importance of exercising caution in the use of household consumer products such as perfumes and air fresheners to mitigate their impact on air quality.

Petunia PHYTOCHROME INTERACTING FACTOR 4/5 transcriptionally activates key regulators of floral scent

Floral scent emission of petunia flowers is regulated by light conditions, circadian rhythms, ambient temperature and the phytohormones GA and ethylene, but the mechanisms underlying sensitivity to these factors remain obscure. PHYTOCHROME INTERACTING FACTORs (PIFs) have been well studied as components of the regulatory machinery for numerous physiological processes. Acting redundantly, they serve as transmitters of light, circadian, metabolic, thermal and hormonal signals. Here we identified and characterized the phylogenetics of petunia PIF family members (PhPIFs). PhPIF4/5 was revealed as a positive regulator of floral scent: TRV-based transient suppression of PhPIF4/5 in petunia petals reduced emission of volatiles, whereas transient overexpression increased scent emission. The mechanism of PhPIF4/5-mediated regulation of volatile production includes activation of the expression of genes encoding biosynthetic enzymes and a key positive regulator of the pathway, EMISSION OF BENZENOIDS II (EOBII). The PIF-binding motif on the EOBII promoter (G-box) was shown to be needed for this activation. As PhPIF4/5 homologues are sensors of dawn and expression of EOBII also peaks at dawn, the prior is proposed to be part of the diurnal control of the volatile biosynthetic machinery. PhPIF4/5 was also found to transcriptionally activate PhDELLAs; a similar positive effect of PIFs on DELLA expression was further confirmed in Arabidopsis seedlings. The PhPIF4/5–PhDELLAs feedback is proposed to fine-tune GA signaling for regulation of floral scent production.

Production of Body Perfume Using Lemongrass Essential Oil

The aim of this study is the extraction of essential oil from lemongrass and its utilization in the production of body perfume. The lemongrass samples used in the study were collected from Eke-Awka market Anambra State, Nigeria and were oven dried, grinded into powder and the oil extraction was carried out using soxhlet extractor with Normal hexane as solvent in the extraction process while AOAC, 2010 method of Analysis was employed in the determination of the physiochemical properties of the oil. The result obtained from the analysis showed that orange peel has a light yellow oil with percentage yield of 1.67% pH of 5.5, refractive index of 1.0321, specific gravity of 0.94, Saponification value of 527.35mgKOH/g, peroxide value of 17mgKOH/kg, iodine value of 20.20gI/100g, and acid value of 33.34mgKOH. 100ml of lemongrass essential oil extracted were used in the production of perfume while 100ml of Methanol and 100ml of the Fixatives were added to the mixture (to improve the longevity of the perfume), and finally the solution were shaken, allowed to stand for 48hrs and poured into different 50ml spray bottles. The result of the comparative analysis of the lemongrass oil body perfume and commercial body perfume showed the ph as 6.8 and 6.1, and the lemongrass oil body perfume lasted for 8hrs when sprayed. Lemongrass oil body perfume competes favorably with commercial available body perfume.