Performed Pearson Correlation Research Articles

The Spatiotemporal Characteristics and Driving Factors of Soil Degradation in the Black Soil Region of Northeast China

The Northeastern Black Soil Region in China is recognized as one of the three major black soil regions globally and is often regarded as a cornerstone of national food security. However, prolonged agricultural practices have led to increasingly severe soil degradation, and the mechanisms and driving factors behind the degradation of soil quality remain unclear. Therefore, this study examines the historical and current characteristics of soil quality, focusing on major influencing factors, such as the 70-year history of reclamation and climate change. By accessing different databases, reviewing the relevant literature, and performing Pearson correlation and redundancy analyses (RDA), this study investigated the variation patterns of significant soil quality indicators and their driving factors in the 0–20 cm soil layer along the latitudinal direction (Nenjiang, Beian, Hailun, and Harbin) in the typical black soil region of Northeast China. The main conclusions are as follows: the soil organic matter (SOM) content experienced a rapid decline in the 30 years preceding cultivation (1950~1980), with the greatest decline rate in the Beian area (about 1.10 g/kg per year). The SOM in the Beian, Hailun, and Harbin areas decreased from north to south, changing at rates of 9.40–21.67 g/kg/degree and 0.15–0.34 g/kg/m with latitude and elevation, respectively. Elevation impacts the annual rate of change in soil quality indicators through its influence on the annual mean maximum temperature (AMXT) and annual atmospheric pressure (AP). AMXT and AP exhibit a linear relationship with elevation, based on which regression models were established. The key factors influencing soil quality indicators in the black soil region include cultivation years (Y), annual mean maximum and minimum temperatures (AMXT and AMNT), annual relative humidity (ARH), and AP. An increase in chemical fertilizer application is among the critical factors affecting soil pH. Additionally, the extensive use of agricultural machinery can reduce soil porosity and cause water and salt accumulation, ultimately leading to a decline in soil pH. This study offers theoretical support for mitigating soil degradation in Northeast China’s black soil region, thereby contributing to national food security and promoting sustainable development.

Mechanisms of intestinal DNA damage and inflammation induced by ammonia nitrogen exposure in Litopenaeus vannamei

Ammonia nitrogen, a common aquaculture pollutant, harms crustaceans by causing intestinal inflammation, though its exact mechanisms are unclear. Thus, we exposed shrimp to 0, 2, 10 and 20 mg/L NH4Cl exposure for 0, 3, 6, 12, 24, 48, 72 h, and explored the intestinal stress, apoptosis, proliferation, inflammation and its histopathological changes. This research indicated that ammonia nitrogen exposure heightens plasma dopamine (DA), 5-hydroxytryptamine (5-HT), norepinephrine (NE), and acetylcholine (ACh) levels, alters gene expression of neurotransmitter receptors in the intestine, triggering the PLCCa2+ pathway and induces endoplasmic reticulum stress. Additionally, mitochondrial fission-related genes (Drp1, FIS1) significantly increase, the level of reactive oxygen species (ROS) was significantly elevated in the intestine, which induced DNA damage effects and initiated the DNA repair function, mainly through the base excision repair pathway, but with a low repair efficiency. By determining the expression of key genes of caspase-dependent and non-caspase-dependent apoptotic pathways, it was found that ammonia nitrogen exposure induced apoptosis in intestinal cells, proliferation key signaling pathways such as Wnt, EGFR and FOXO signaling showed an overall decrease after ammonia nitrogen exposure, combined with the gene expression of cell cycle proteins and proliferation markers, indicated that the proliferation of intestinal cells was inhibited. Performing pearson correlation analysis of intestinal cell damage, proliferation, and inflammatory factors, we hypothesized that ammonia nitrogen exposure induces intestinal endoplasmic reticulum stress and mitochondrial fission, induces elevated ROS, leads to DNA damage, and causes inflammation and damage in intestinal tissues by the underlying mechanism of promoting apoptosis and inhibiting proliferation.

Altered executive control network and default model network topology are linked to acute electronic cigarette use: A resting‐state fNIRS study

AbstractIn recent years, electronic cigarettes (e‐cigs) have gained popularity as stylish, safe, and effective smoking cessation aids, leading to widespread consumer acceptance. Although previous research has explored the acute effects of combustible cigarettes or nicotine replacement therapy on brain functional activities, studies on e‐cigs have been limited. Using fNIRS, we conducted graph theory analysis on the resting‐state functional connectivity of 61 male abstinent smokers both before and after vaping e‐cigs. And we performed Pearson correlation analysis to investigate the relationship between alterations in network metrics and changes in craving. E‐cig use resulted in increased degree centrality, nodal efficiency, and local efficiency within the executive control network (ECN), while causing a decrease in these properties within the default model network (DMN). These alterations were found to be correlated with reductions in craving, indicating a relationship between differing network topologies in the ECN and DMN and decreased craving. These findings suggest that the impact of e‐cig usage on network topologies observed in male smokers resembles the effects observed with traditional cigarettes and other forms of nicotine delivery, providing valuable insights into their addictive potential and effectiveness as aids for smoking cessation.

Using structural equation modeling to examine correlations between health-related physical fitness and cell health among Chinese college students

IntroductionCollege students’ physical fitness is likely to be directly related to their cells’ health. However, there is a lack of literature on whether the relationship between cell health and college students’ physical fitness is direct or indirect. This study used a structural equation modeling (SEM) approach to investigate the connection between cell health and college students’ physical fitness.MethodsThis cross-sectional study collected data from 838 volunteers (502 males and 336 females, average age of 18.74 ± 1.5 years) who were college students from the Shandong province of China in July 2023. Initially, we obtained anthropometric measurements and conducted physical fitness tests on the students. Then, we performed Pearson correlation analysis and principal component analysis to screen variables and explore potentially influencing factors. Finally, we examined associations between the variables and determined whether there were direct or indirect influences among factors using SEM.ResultsThe results revealed a significant correlation between the cell health factor and the muscle strength factor (path coefficient = 0.97; p < 0.001) as well as the fat obesity factor (path coefficient = -0.52; p < 0.001). The cardiovascular factor exhibited a weak correlation with the cell health factor (path coefficient = 0.11; p < 0.01). Moreover, the cardiovascular factor acted as a mediating variable between the muscle strength factor and the cell health factor, with a positive correlation observed between the muscle strength factor and the cell health factor (path coefficient = 0.40; p < 0.001).ConclusionThese findings suggest that cell health is indicative of muscle strength and cardiorespiratory fitness. Our findings demonstrate that assessing the cell health of college students can be a valuable method for evaluating their overall health.

Body Adiposity Index as a Predictor of Body Fat in an Oldest Old and Independent Cohort of Brazilian Older Adults.

Anthropometry and body mass index (BMI) do not assess body composition or its distribution in older adults; thus, individuals may have different fat percentages but similar BMI values. The body adiposity index (BAI) was recently proposed as a feasible and inexpensive method for estimating the percentage of body fat based on measurements of hip circumference and height. The present study evaluated whether BAI and BMI are useful alternatives to dual-energy X-ray absorptiometry (DXA), which is rarely used in clinical practice, for predicting body fat in independent long-lived older adults. In this cross-sectional study, we used DXA to calculate the percentage of body fat, which was compared with BAI and BMI values. We performed Pearson correlation analyses and used Cronbach's alpha, described by Bland and Altman, to compare the reliability between the indexes. Among 157 evaluated individuals (73.2% women, mean age 87±3.57 years), men had a lower percentage of total fat, as assessed by DXA, and lower BAI indices than women. The correlation between BAI and DXA was moderate (r=0.59 for men and r=0.67 for women, p&lt;0.001). We confirmed the reliability based on Cronbach's alpha coefficients of 0.67 in men and 0.77 in women. We also observed that the BAI was strongly positively correlated with BMI in both men and women. The BAI, used in combination with BMI, can be an alternative to DXA for the assessment of body fat in the oldest old in clinical practice, mainly women, and can be used to add information to BMI.

Transcriptome-wide analysis uncovers regulatory elements of the antennal transcriptome repertoire of bumblebee at different life stages.

Bumblebees are crucial pollinators, providing essential ecosystem services and global food production. The success of pollination services relies on the interaction between sensory organs and the environment. The antenna functions as a versatile multi-sensory organ, pivotal in mediating chemosensory/olfactory information, and governs adaptive responses to environmental changes. Despite an increasing number of RNA-sequencing studies on insect antenna, comprehensive antennal transcriptome studies at the different life stages were not elucidated systematically. Here, we quantified the expression profile and dynamics of coding/microRNA genes of larval head and antennal tissues from early- and late-stage pupa to the adult of Bombus terrestris as suitable model organism among pollinators. We further performed Pearson correlation analyses on the gene expression profiles of the antennal transcriptome from larval head tissue to adult stages, exploring both positive and negative expression trends. The positively correlated coding genes were primarily enriched in sensory perception of chemical stimuli, ion transport, transmembrane transport processes and olfactory receptor activity. Negatively correlated genes were mainly enriched in organic substance biosynthesis and regulatory mechanisms underlying larval body patterning and the formation of juvenile antennal structures. As post-transcriptional regulators, miR-1000-5p, miR-13b-3p, miR-263-5p and miR-252-5p showed positive correlations, whereas miR-315-5p, miR-92b-3p, miR-137-3p, miR-11-3p and miR-10-3p exhibited negative correlations in antennal tissue. Notably, based on the inverse expression relationship, positively and negatively correlated microRNA (miRNA)-mRNA target pairs revealed that differentially expressed miRNAs predictively targeted genes involved in antennal development, shaping antennal structures and regulating antenna-specific functions. Our data serve as a foundation for understanding stage-specific antennal transcriptomes and large-scale comparative analysis of transcriptomes in different insects.

Pan-cancer integrated analysis of ANKRD1 expression, prognostic value, and potential implications in cancer

There is substantial evidence demonstrating the crucial role of inflammation in oncogenesis. ANKRD1 has been identified as an anti-inflammatory factor and is related to tumor drug resistance. However, there have been no studies investigating the prognostic value and molecular function of ANKRD1 in pan-cancer. In this study, we utilized the TCGA, GTEx, GSCALite, ENCORI, CTRP, DAVID, AmiGO 2, and KEGG databases as well as R language, to explore and visualize the role of ANKRD1 in tumors. We employed the ROC curve to explore its diagnostic significance, while the Kaplan–Meier survival curve and Cox regression analysis were used to investigate its prognostic value. Additionally, we performed Pearson correlation analysis to evaluate the association between ANKRD1 expression and DNA methylation, immune cell infiltration, immune checkpoints, TMB, MSI, MMR, and GSVA. Our findings indicate that ANKRD1 expression is dysregulated in pan-cancer. The ROC curve revealed that ANKRD1 expression is highly sensitive and specific in diagnosing CHOL, LUAD, LUSC, PAAD, SKCM, and UCS (AUC > 85.0%, P < 0.001). Higher ANKRD1 expression was related to higher overall survival (OS) in LGG, but with lower OS in COAD and STAD (P < 0.001). Moreover, Cox regression and nomogram analyzes suggested that ANKRD1 is an independent factor for COAD, GBM, HNSC, and LUSC. Dysregulation of ANKRD1 expression in pan-cancer involves DNA methylation and microRNA regulation. Using the CTRP database, we discovered that ANKRD1 may influence the half-maximal inhibitory concentration (IC50) of several anti-tumor drugs. ANKRD1 expression showed significant correlations with immune cell infiltration (including cancer-associated fibroblast and M2 macrophages), immune checkpoints, TMB, MSI, and MMR. Furthermore, ANKRD1 is involved in various inflammatory and immune pathways in COAD, GBM, and LUSC, as well as cardiac functions in HNSC. In vitro experiments demonstrated that ANKRD1 promotes migration, and invasion activity, while inhibiting apoptosis in colorectal cancer cell lines (Caco2, SW480). In summary, ANKRD1 represents a potential prognostic biomarker and therapeutic target in human cancers, particularly in COAD.

Global Physical Activity, Active Commuting to School, and Sedentary Behavior Among Latin American Adolescents: Global School-Based Student Health Survey and the National School Health Survey.

This study aimed to compare the overall prevalence, stratified by sex and age group of global physical activity (GPA), active commuting to school (ACS), and sedentary behavior (SB) among adolescents from ten Latin American countries, and to assess the correlation of Development Index with the indicators. This research is grounded on data from the Global School-Based Student Health Survey (2009-2015) and the 2015 Brazilian National School Health Survey. The prevalence and 95% confidence intervals for GPA (≥5d/wk), ACS (≥1d/wk), and SB (>2h/d) were calculated using the chi-square test to compare the sexes (male; female) and age group (≤13y; 14y; 15y; ≥16y). We also performed Pearson correlation analysis with the Human Development Index. The prevalence of indicators ranged from 16.1% to 28.2% for GPA, from 56.7% to 71.2% for ACS, and from 20.7% to 62.6% for SB. Boys generally had a higher prevalence of GPA and ACS, and girls had a higher prevalence of SB. The prevalence of the indicators by age group varied between countries, with significant differences observed in some, depending on each indicator. A positive correlation was observed between Human Development Index, GPA, and SB. Health promotion policies must include guidelines that encourage and promote a more active and less sedentary lifestyle among young people in Latin America, considering specific groups, the local socioeconomic context, and differences between countries.

Developing Topics.

The novel diffusion-based Neurite Orientation and Dispersion Imaging (NODDI) model may provide complementary information to Diffusion Tensor Imaging (DTI) to increase sensitivity in characterizing tissue microarchitecture. We examined if one or a combination of both approaches can improve our understanding of at-risk tissue in and around white matter hyperintensities (WMH). From ADNI3, we identified individuals [n = 54, age = 78 ± 7, 46% F) with concurrent measures of multi-shell DTI, 3D FLAIR, and 3D MPRAGE. We segmented WMH from structural data using Lesion Segmentation Toolbox. We derived DTI (FA, MD) and NODDI parameters (NDI, ODI, Fiso) using QSIprep. We dilated up to 5 mm outside the WMH, applied an FA threshold of ≥ 0.2 to exclude gray matter, calculated DTI and NODDI parameters at 1 mm intervals within this region. We performed Pearson correlations between all parameters. Assuming normal white matter at 5 mm, we normalized these parameters with their respective values at 5 mm and plotted the spatial variation of each parameter in and outside the WMH. Normalized DTI and NODDI parameters representing spatial variations within the 5 mm region are shown in Fig 1A. Table 1 shows p-values comparing DTI and NODDI parameters between WMH the subsequent regions outside it. Compared to WMH, Fiso was significantly different at all distances. FA showed similar spatial characteristics with NDI and was negatively correlated with ODI. Fiso correlated very strongly with MD. In the WMHs and its immediate vicinity, low FA, NDI, ODI suggest lower cell density, while low Fiso and MD suggest low extracellular fluid volume. Together these observations may reflect cytotoxic edema, cell loss, and injury. Normal appearing white matter (3-5 mm) showed high FA, NDI, ODI, and low MD consistent with high cell density and normal extracellular water. In the transition between WMH vicinity and normal white matter, low FA, NDI, ODI, high MD and Fiso suggest cell loss only. This transition region could be at risk of converting to WMH. Future work will include longitudinal evaluation of WMH growth in the at-risk tissue. However, final validation of WMH pathology will need histological confirmation.

T-Cells Immune Cluster Analysis Using Cytof Identifies Unique Patient Subgroups with TP53 Mutation and Predicts Complete Remission (CR) in Patients with Acute Myeloid Leukemia (AML) Receiving Venetoclax Plus Hypomethylating Agent Therapy

Introduction The advent of venetoclax (VEN) based chemotherapy has revolutionized the treatment of elderly acute myeloid leukemia (AML), with improvement in remission rates from historical 10%-20% to 60%-70%. However, remission duration is short-lived in patients (pts) with high-risk AML with consequent dismal outcome. As our knowledge of genomic landscape of AML expands and more immunotherapies become available, exploration of the immune tumor microenvironment (TME) of AML may allow identification of subsets of pts that may benefit from immune based therapy. We conducted an investigator initiated prospective study to evaluate TME of pts receiving VEN plus hypomethylating agent (HMA; azacitidine or decitabine) combination. We hypothesize that these pts will cluster into immune-infiltrated vs. immune-depleted sub-types by high-definition immune cell profiling. Methods In 26 newly diagnosed AML pts, who were planned to receive VEN plus HMA therapy, we isolated bone marrow (BM) and peripheral blood (PB) derived mononuclear cells (MC) by density gradient centrifugation and cryopreserved. We performed CyTOF (cytometry time-of-flight) analysis to study their immune signatures. Thawed cells were labeled with metal-conjugated antibodies that bind CD33, CD196, CD19, CD127, CD11b, CD4, IgD, CD11c, CD14, CD56, CD123, TCRgd, CD185/CXCR5, CD45RA, CD27, CD28, CD66b, CD183 (CXCR3), CD161, CD45RO, CD197 (CCR7), CD8a, CD25, CD3, CD20, CD38, HLA-DR, CD194 (CCR4), CD57, CD16 and CD45. We performed a Rphenograph clustering on 25 paired BMMC and PBMC samples (one patient only had PBMC) to create 35 unique clusters for each ( Figure 1). Then we broke down the distribution of cluster by two different groupings: pts with TP53 mutation (m) and those who achieve complete remission. Statistical significance of clusters calculated using t-test. We performed Pearson correlation test on the paired BMMC and PBMC samples to assess correlation between BMMC and PBMC samples. Results Baseline characteristics The median age of the pts was 74 years (range, 53-88), and 17 (65%) pts were male. Ten (37%) pts had secondary AML, and 12 (46%) pts had ELN adverse risk disease (n=6; TP53m). There was no significant difference in median age (p= &amp;gt;0.99), proportion of pts with secondary AML (p= 0.64), acute myelomonocytic leukemia (p= 0.14) white blood cell count (WBC) ≥ 100 (10 9/L) (p= &amp;gt;0.99), and frequency of commonly occurring co-mutations ( ASXL1 [p=0.35]), FLT3 ITD [p=0.54], splicing [p=0.29], IDH1/2 [p=0.28], DNMT3A [p=0.29], NPM1 [p=0.29], TET2 [p=0.35]) in TP53m and TP53 wild-type group. Response 12/20 (60%) evaluable pts achieved complete remission with or without count recovery (CR/CRi). There was no significant difference in median age (p= 0.06), proportion of pts with secondary AML (p= 0.16), therapy related AML (p= 0.60) WBC ≥ 100 (10 9/L) (p= 0.40), and frequency of commonly occurring mutations ( TP53 [p=0.60], ASXL1 [p &amp;gt; 0.99]), FLT3 ITD [p=0.54], splicing [p &amp;gt; 0.99], IDH1/2 [p=0.60], DNMT3A [p=&amp;gt;0.99], NPM1 [p=0.61], TET2 [p &amp;gt;0.99]) in CR/CRi and no-CR/CRi group. CyTOF analysis and Rphenograph clustering on 25 paired BMMC and PBMC samples We found significant clustering with high expression of B-cell activated (cluster 2; p= 0.005), NK cell rich (cluster 1; p= 0.005, cluster 35; p= 0.0009), CD8 T-cells (cluster 31; p= 0.01) and CD4 effector cells (cluster 17; p= 0.005) phenotype among TP53m AML ( Table 1). Among pts who achieved CR/CRi, significant clustering with low expression of B-cell activated (cluster 29; p= 0.01), CD8 cytotoxic (cluster 14; p= 0.01) CD4 activated (cluster 21; p= 0.03) phenotypes observed. Correlation between BMMC and PBMC clustering Among clusters which showed significance for a particular phenotype, we performed Pearson correlation (R 2) and observed strong correlation between BMMC and PBMC samples clustering in cluster 1 (R 2 = 0.99), cluster 2 (R 2 = 0.95), cluster 17 (R 2 = 0.96), cluster 21 (R 2 = 0.99) and cluster 29 (R 2 = 0.93). Conclusion In this single institutional study using CyTOF analysis we have identified novel clustering with immune- infiltrated phenotype among pts with TP53m AML and conversely immune-depleted phenotype among AML pts who responded to VEN plus HMA. Moreover, we found strong correlation in CyTOF analysis conducted from BMMC and PBMC samples. Future studies with larger sample size needed to validate our findings.

Natural Radioactivity of Granitic Rocks and Their Health Risk around Ingesana Mountains, Sudan.

This work was carried out mainly to analyze radioactive elements 40 K, 232 Th, and 226 Ra and non-radioactive elements in some granitic rocks and study their health risk for humans and non-humans. Radioisotope activity was evaluated using a high-purity germanium (HPGe) detector, and various radiological hazard indices were calculated. We also measured some non-radioactive elements using x-ray fluorescence analysis and performed Pearson correlation analysis to examine the relationships between the radionuclides and the non-radioactive elements. Furthermore, we used the ERICA software to estimate the total dose rate per organism for some non-human biota. The means of 226 Ra, 232 Th, and 40 K are 25.13 ± 5.22 Bq kg -1 , 29.01 ± 6.95 Bq kg -1 , and 323.07 ± 97.83 Bq kg -1 , respectively. Hence, 226 Ra, 232 Th, and 40 K radioactivities were lower than the worldwide average limits. The 226 Ra, 232 Th, and 40 K levels were under the global average values. The hazardous radiometric variables were computed, and the findings observed that the mean absorbed dosage was 23.48 ± 7.36 nGy h -1 , the yearly effective dose rate was 29.82 ± 10.46 μSv y -1 , and the radium equivalent activity was 88.88 ± 18.64 Bq kg -1 . The average dose and yearly effective dose rates were lower than the global average absorbed dose rate of 60 nGy h -1 and the ICRP standard of 1.0 mSv y -1 . The results of Ra-equivalent activity were lower than the recommended maximum criterion for building materials limit of Ra-equivalent activity (370 Bq kg -1 ) proposed by UNSCEAR. Tests of Pearson correlation coefficients indicate a substantial positive relationship between 226 Ra and 232 Th and a modest correlation between 226 Ra and 40 K. Meanwhile, no association between 226 Ra and 40 K and non-radioactive components has been identified. Thorium-232 exhibits a moderately favorable connection with Ti, Fe, and Ni. The estimated total dose rate per organism for the studied non-human is less than the ambient dose rate. However, the total dose rate for small burrowing mammals and reptiles exceeds the background exposure rate. In accordance with the findings, granite rocks may be safe to use as construction materials and pose no threat to human health.

Cortical activity and spatiotemporal parameters during gait termination and walking: A preliminary study

Gait termination requires an interaction between the biomechanical and neuromuscular systems to arrest forward momentum. Currently, the biomechanical characteristics of gait termination have been demonstrated; however, the neural mechanism of gait termination remains unclear. This study aimed to investigate cortical activity during gait termination using functional near-infrared spectroscopy (fNIRS). Thirteen healthy younger adults (mean age:24.0 ± 1.7) participated in this study. All participants performed three experimental sessions: planned gait termination (PGT), unplanned gait termination (UGT), and walking. Each experimental session comprised a block paradigm design (three cycles; 20 s resting, 45 s task). Cortical activity in the dorsolateral prefrontal cortex (DLPFC), supplementary motor area (SMA), and primary motor cortex (M1) and spatiotemporal parameters were measured. We compared the cortical activities and spatiotemporal parameters among PGT, UGT, and walking sessions. In addition, we performed Pearson correlations between hemodynamic responses and spatiotemporal parameters. The PGT was activated in the right DLPFC, whereas the UGT and walking were activated in the left SMA (p < 0.05). Comparing cortical activation between sessions, both the PGT and UGT showed significantly higher activation in the right DLPFC than during walking (p < 0.05). There were no significant differences in cortical activity between PGT and UGT (p > 0.05). In addition, the gait termination time revealed moderate positive correlation with hemodynamic responses in the right DLPFC (p < 0.05). Our results indicate that the right DLPFC is associated with gait termination, regardless of gait termination type. Our findings provide the potential implication that the hemodynamic response in the right DLPFC would be a biomarker to evaluate the ability of gait termination.

Glucose metabolism in posterior cingulate cortex has supplementary value to predict the progression of cognitively unimpaired to dementia due to Alzheimer's disease: an exploratory study of 18F-FDG-PET.

Amyloid-β (Aβ) and tau are important biomarkers to predict the progression of cognitively unimpaired (CU) to dementia due to Alzheimer's disease (AD), according to the diagnosis framework from the US National Institute on Aging and the Alzheimer's Association (NIA-AA). However, it is clinically difficult to predict those subjects who were already with Aβ positive (A +) or tau positive (T +). As a typical characteristic of neurodegeneration in the diagnosis framework, the hypometabolism of the posterior cingulate cortex (PCC) has significant clinical value in the early prediction and prevention of AD. In this paper, we proposed the glucose metabolism in the PCC as a biomarker supplement to Aβ and tau biomarkers. First, we calculated the standard uptake value ratio (SUVR) of PCC based on fluorodeoxyglucose positron emission computed tomography (FDG PET) imaging. Secondly, we performed Kaplan-Meier (KM) survival analyses to explore the predictive performance of PCC SUVR, and the hazard ratio (HR) was calculated. Finally, we performed Pearson correlation analyses to explore the physiological significance of PCC SUVR. As a result, the PCC SUVR showed a consistent downward trend along the AD continuum. KM analyses showed better predictive performance when we combined PCC SUVR with cerebro-spinal fluid (CSF) Aβ42 (from HR = 2.56 to 3.00 within 5years; from HR = 2.76 to 4.20 within 10years) and ptau-181 (from 2.83 to 3.91 within 5years; from HR = 2.32 to 4.17 within 10years). There was a slight correlation between Aβ42/Aβ40 and PCC SUVR (r = 0.14, p = 0.02). In addition, several cognition scales were also correlated to PCC SUVR (from r = -0.407 to 0.383, p < 0.05). Our results showed that glucose metabolism in PCC may be a potential biomarker supplement to the Aβ and tau biomarkers to predict the progression of CU to AD.

Correlations among chromosome numbers, nuclear DNA contents and climatic variables of South American Cactaceae in a phylogenetic context

Abstract Cactaceae are American members of arid and semi-arid regions and are also present in tropical forests and temperate climates. The taxonomy is complex owing to large morphological variability, and polyploidy is a significant mechanism in cactus speciation associated with geographical and environmental range expansions. In South America, Cactaceae have a wide distributional range and provide an informative model in which to examine the correlation between ploidy levels and genome sizes with geographical and bioclimatic variables. We tested whether ploidy levels and DNA contents are related to elevation and the 19 WorldClim variables, and we mapped chromosome numbers and amounts of DNA on a molecular phylogeny to interpret their evolution. We performed Pearson correlation tests between the response variables and each climatic variable and used a multivariate analysis to assess the pattern of variation among variables and chromosome numbers. Our data suggest that polyploidy had independent origins within each subfamily. Chromosome numbers were related to variables associated with temperature, whereas DNA contents were related mostly to precipitation. A negative correlation was observed between the amount of DNA in one chromosome set and the increase in the ploidy level. Based on the results, the small genome can be considered ecologically important as an adaptation to higher temperatures and droughts.

Insights into abusive workplace behavior.

Insights into abusive workplace behavior.

Assessment of Anxiety among People with Various Physical Activity Levels during Lockdown in Karachi, Pakistan.

Background The purpose of this study is to make it easier to counsel the general public on how to promote their psychological health and better lifestyle by boosting their physical activity in the event of more devastating future waves of coronavirus disease 2019. Coronavirus disease is a viral infection first reported in Wuhan, China, which led to a worldwide pandemic and lockdown. In addition to physical health, the uncertainty of situation and fear of infection have further affected the mental health of the individuals. Lockdown has also halted the physical activity of people further adding into the stress and anxiety. Methods A sample size of 376 was required, but a convenient sampling technique collected 400 responses due to overwhelming Participation. A well-structured questionnaire was used for data collection, which contained sections such as a demographics section. We used the International Physical Activity Questionnaire scale to measure various physical activity levels and the generalized anxiety disorder scale to measure anxiety levels, because of their considerate validity and feasibility. Later both of our variables were compared to find out the correlation of anxiety levels with different physical activity levels. Results Mean Anxiety and its prevalence rates in the sample were 7.32 ± 5.767 and 33%. On performing Pearson correlation, increasing age was found to be a risk factor for Anxiety. One-way analysis of variance showed a significant difference between Physical Activity and Anxiety. In the post-hoc Tukey test, a considerable difference in anxiety level was found between people with no physical activity and vigorous physical activity. Conclusion During the lockdown period, the mean anxiety was found to be 7.0 ± 5.56 with prevalence rate of 33% among the people with no, low, moderate or vigorous physically active levels was found to be associated with better perceived mental health status which suggests the need to promote physically active style to cope with anxiety and awareness sessions to be conducted especially among those who could not maintain a physically active lifestyle. Also, among the general population e-Health programs could provide an appropriate way to promote a physically active lifestyle.

The Influence of Psychosocial Factors on Disability and Expected Improvement Before Surgery for Adult Traumatic Brachial Plexus Injury

Psychosocial factors influence pain and recovery after extremity trauma and may be targets for early intervention. This may be of particular interest for patients with adult traumatic brachial plexus injury (BPI), given the broad and devastating impact of the injury. We hypothesized that there would be an association between depressive symptoms, anxiety, and pain interference with preoperative disability and expectations for improvement after BPI surgery. We enrolled 34 patients into a prospective multicenter cohort study for those undergoing surgery for adult traumatic BPI. Before surgery, participants completed Patient-Reported Outcome Measurement Information System scales for pain interference, anxiety, and depressive symptoms, and a validated BPI-specific measure of disability and expected improvement. We performed Pearson correlation analysis between pain interference, anxiety symptoms, and depressive symptoms with (A) disability and (B) expected improvement. We created separate linear regression models for (A) disability and (B) expected improvement including adjustment for severity of plexus injury, age, sex, and race. Among 34 patients, there was a moderate, statistically significant, correlation between preoperative depressive symptoms and higher disability. This remained significant in a linear regression model adjusted for severity of plexus injury, age, sex, and race. There was no association between severity of plexus injury and disability. Depressive symptoms also were moderately, but significantly, correlated with higher expected improvement. This remained significant in a linear regression model adjusted for severity of plexus injury, age, sex, and race. Depressive symptoms are associated with greater disability and higher expected improvement before BPI surgery. Screening for depressive symptoms can help BPI teams identify patients who would benefit from early referral to mental health specialists and tailor appropriate expectations counseling for functional recovery. We did not find an association between severity of BPI and patient-reported disability, suggesting either that the scale may lack validity or that the sample is biased. Prognostic II.

The Effect of Work Stressors on RN Exhaustion: The Role of Perceived Organizational Support.

This study examines the effect of work environment stressors on RNs' exhaustion through perceived organizational support (POS). Drawing on conservation of resources theory and POS theory, POS is theorized to act as a buffer or mediator between lack of COVID-related support and communication (LCSC), 3 role stressors (role overload, role ambiguity, nonparticipation), bullying, and exhaustion. A cross-sectional survey design was used. A total of 169 questionnaires (16.6% response rate) were analyzed, performing Pearson correlation, mediation, moderation, and hierarchical robust regression tests. The 3 role stressors were strongly related to bullying. Support was found for an interactive relationship between LCSC and POS at different levels of bullying. Role overload, role ambiguity, and bullying had positive direct effects on exhaustion, whereas POS had negative direct and indirect effects. Cognitive skills training at individual level coupled with organizational-based stress interventions and leadership development programs could reduce exhaustion.

Evaluation of Sleep Quality of Anesthesiologists Working in Turkey

Objective: Sleep is one of the basic daily activities that are important for an individual with physical, mental, social, and intellectual needs to be in good physical and mental health. Although many definitions are made, sleep; is a complex and physiological event affected by pathophysiological, physical, psychological, and environmental factors. Anesthesiologists often work in closed environments and with long working hours, which can cause severe, chronic sleep loss and sleep disruption. Sleeplessness and low sleep quality can cause decreased attention during the day, impaired judgment, and delays in decision-making. This study, it is aimed to determine the sleep quality of Anesthesiology and Reanimation specialists working in Turkey. Methods: The study included all anesthesiologists whose e-mail addresses were registered with the Turkish Society of Anesthesiology and Reanimation and who worked in Turkey. Survey questions, which the participants can answer electronically, were sent to the participant's e-mail addresses. To increase the number of participants, a reminder email was sent three weeks after the first email. The data were expressed as a number, percentage, mean, and standard deviation and the correlation between the parameters will be evaluated at a 95% confidence interval by performing Pearson correlation analysis; p&amp;lt; 0.05 was accepted as significant. Results: Due to seven people with sleep disorders being excluded from the study, 210 out of 217 people who answered the questionnaires sent by email to the anesthetists who are members of the Turkish Society of Anesthesiology and Reanimation were included in the study. Of the respondents who worked in the following fields: 30% (n = 63) State Hospital, 33.3% (n = 70) University Hospital, 17.1% (n = 36) Ministry of Health Affiliate Hospital, 19.5% (n = 41) Private Hospital, and 73.3% (n = 154) at the Operating Room, 10% (n = 21) at the Intensive Care, 15.2% (n = 32) at the Operating Room + Intensive Care, and 1.4% (n = 3) at other departments, 48.4% (n = 105) were male, 51.6% (n = 112) were female, with a mean age of 41.5±7.6 years. The average hourly shift for the participants, 61% of whom were on the night shift, was 50 hours per month, and 25% were working more than 45 hours per week. Conclusion: The sleep quality of anesthesiologists is poor, which causes daytime dysfunction. It is thought that the reason for poor sleep quality, in general, is the adverse effects of their duties and responsibilities, working environment, and operating conditions on sleep quality.

Comprehensive analysis of prognosis of patients with GBM based on 4 m6A-related lncRNAs and immune cell infiltration

ObjectiveTo investigate the immune cell infiltration status in glioblastoma multiforme (GBM) and construct a novel prognostic risk model that can predict patients’ prognosis. MethodsThe Cancer Genome Atlas (TCGA) database was used to obtain RNA-sequence information and relevant clinical data. We performed Pearson correlation, univariate Cox regression to screen m6A-related prognostic lncRNA. GMB patients’ samples were separated into different clusters through the ConsensusClusterPlus package. The risk score model was established through LASSO regression analysis. Besides, KEGG pathway enrichment analysis was implemented. CIBERSORT algorithm was used to analyze the difference of 22 types of immune cell infiltration in different cluster of GBM patient. Cox regression analyses were used to verify the independence of the model and correlation analysis was performed to demonstrate the link between our model and clinical characteristics of GBM patients. Experiments were used to validate the differential expression of the model lncRNA in patients with different prognosis. Results17 lncRNA related to prognosis were screened from 1021 m6A-related lncRNAs. Further, four m6A-related lncRNAs that were significantly correlated with GBM prognosis were selected to establish our prognostic risk model, which had excellent accuracy and can independently predict the prognosis of GBM patients. The infiltration fractions of T regulatory cells, T cells CD4 memory activated and neutrophils were positively associated with risk score, which suggested a significant relationship between the model and tumor immune microenvironment. ConclusionThe m6A-related RNA risk model offered potential for identifying biomarkers of therapy and predicting prognosis of GBM patients.