ABSTRACT Introduction Soccer is a sport with intense rivalry, where physical strength is a prerequisite to ensure high level of playability. Objective Explore the application of functional training in soccer training and its influence on soccer players’ specific physical fitness. Methods 24 s and divided into experimental and control groups into an experimental group and a control group. The control group underwent the intervention according to the normal program, while the experimental group used a functional training program protocol. The subjects’ FMS scores and special fitness and ss were tested before and after the experiment, the results were statistically analyzed and discussed in the light of up-to-date scientific literature. Results Before the experiment, the overall functional action screening scores of the two groups of players were low, and there was a high risk of sports injuries. After 12 weeks of training intervention, the experimental and control groups’ FMS scores were significantly elevated, indicating that traditional physical and functional training can effectively improve functional movement screening performance. Conclusion The 12 weeks suggested functional training protocol can significantly improve the physical fitness of soccer players. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.