To investigate how contextual or environmental factors may influence the athletic performance of female soccer players during competitions, this study aimed to assess the impact of match-related variables (playing surface; opponent levels; opponent result trends; match status, and match outcomes) on the athletic performance of professional female soccer athletes. Seventeen athletes (25.5 ± 4.3 years of age) from the same team competing in the Italian second division were divided into two groups according to their roles and athletic characteristics: Group A (central defenders and forwards) and Group B (right/left full-backs and midfielders). Total distance (TD) and sprint distance (SD); high-speed running (HSR), acceleration (ACC), and deceleration numbers (DEC); average and maximal heart rate (HR_avg; HR_max); and match-related factors were collected during the 22 regular season matches. A T-test and ANOVA were used to calculate the differences between groups in GPS and HR variables and the effects of match-related factors, respectively. Results showed higher running performance in the B group compared to the A group during matches. Increased ACC number was seen in matches played on artificial turf; winning was associated with decreased HR_max and increased DEC number. Athletes covered higher TD reaching lower HR_max when playing against lower-ranking opponents while matches lost/drawn resulted in higher HSR. High HRs (max and avg) were found when playing against a team with a positive performance trend. In summary, match-related factors and opponents' characteristics can affect athletic performance in female soccer athletes. These factors should be considered during in-seasonal training to personalize match preparation and optimize athlete performance.