In order to clarify the pollution characteristics and human health risks of PFASs pollutants in typical drinking water sources in Zhejiang Province, this study relies on ultra -performance liquid chromatography-mass spectrometry (UPLC-MS/MS) technology to analyze the pollution of 26 PFASs in 7 reservoirs in Zhejiang Province. The detected concentrations of PFASs were evaluated to further assess the human health risks. Total PFASs concentrations in the seven reservoirs ranged from 1.30 ng L−1–24.90 ng L−1. Among the 26 PFASs pollutants analyzed, PFOA and PFBA were the main PFASs pollutants, the detected concentrations of PFOA and PFBS ranging from 0.50 ng L−1–13.70 ng L−1 and 0 ng L−1–1.70 ng L−1, respectively. Then we evaluated 15 PFASs and calculated the results of the HQ value of the reproductive toxicity and hepatotoxicity of the total PFASs in this study ranged from 2.30 × 10–8 to 1.16 × 10–4 and 9 × 10–8 to 5.24 × 10–4 respectively, which were both lower than 0.01, indicating that there is no significant risk to the human body.