An Ad hoc network is a collection of wireless mobile hosts forming a temporary network without the aid of any centralized administration or standard support services. MANET can be defined using unstable network infrastructure, self-organizing network topology and independent node mobility. This becomes obtainable due to their routing techniques; in other terms, routing is a backbone for MANET. However, due to network load routing performance of MANET is degraded thus, some optimization on network routing strategy is required. In this paper, we introduce a new technique by using the concept of Genetic algorithm (GA) with AODV Protocol to make routing decision in computer network. The goal of this paper is to find the optimal path between the source and destination nodes and increased the QoS and Throughput. We implemented and compare this a new technique with the traditional AODV, and we shows that the new technique is better performance than the traditional AODV.