Growth Performance Research Articles

Exogenous diethyl aminoethyl hexanoate alleviates the damage caused by low-temperature stress in Phaseolus vulgaris L. seedlings through photosynthetic and antioxidant systems

BackgroundPhaseolus vulgaris is a warm-season crop sensitive to low temperatures, which can adversely affect its growth, yield, and market value. Exogenous growth regulators, such as diethyl aminoethyl hexanoate (DA-6), have shown potential in alleviating stress caused by adverse environmental conditions. However, the effects that DA-6 has on P. vulgaris plants subjected to low-temperature stress are not well understood. This study aimed to investigate the impact DA-6 has on the growth, photosynthesis, antioxidant system, and gene expression in cold-tolerant (YJ009763) and cold-sensitive (Baibulao) P. vulgaris seedlings under low-temperature stress.ResultsTo simulate low-temperature stress, P. vulgaris seedlings were exposed to 5 °C, and 25 mg/L DA-6 solution applied to their leaves. This study revealed that DA-6 significantly enhanced the growth and photosynthetic performance of P. vulgaris seedlings under low-temperature stress. Specifically, DA-6 increased chlorophyll content and photosynthetic rates, reducing stomatal limitation and enhancing carbon assimilation. It also improved the photosynthetic efficiency by boosting electron transport in the reaction center. The antioxidant enzyme activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) were markedly increased following DA-6 treatment. After 24 h of low-temperature stress, the cold-tolerant seedlings showed a 68.95% increase in POD activity, whereas the cold-sensitive seedlings displayed a 160.63% increase in SOD activity and an 85.56% increase in CAT activity. In addition, DA-6 significantly reduced the production rate of superoxide anion radical generation, with a 25.24% reduction in cold-tolerant seedlings and a 49.38% reduction in cold-sensitive seedlings. Under low-temperature stress, exogenous DA-6 could upregulate the relative expression of antioxidant enzyme-related genes, such as PvSOD and PvAPX. DA-6 also promoted the expression of key antioxidant genes, including PvMDHAR and PvDHAR2, which accelerated the ascorbate-glutathione cycle and mitigated oxidative stress.ConclusionExogenous application of DA-6 effectively alleviates low-temperature stress in P. vulgaris by enhancing photosynthetic capacity and regulating the antioxidant defense system. Cold-tolerant varieties exhibited a stronger response to DA-6, demonstrating a greater ability to withstand cold stress. These findings suggest that DA-6 treatment could serve as a promising approach for improving the resilience of P. vulgaris to low temperatures.

Aflatoxin B1: Challenges and Strategies for the Intestinal Microbiota and Intestinal Health of Monogastric Animals

The objective of this review is to investigate the impacts of aflatoxins, particularly aflatoxin B1 (AFB1), on intestinal microbiota, intestinal health, and growth performance in monogastric animals, primarily chickens and pigs, as well as dietary interventions to mitigate these effects. Aflatoxin B1 contamination in feeds disrupts intestinal microbiota, induces immune responses and oxidative damage, increases antioxidant activity, and impairs jejunal cell viability, barrier function, and morphology in the small intestine. These changes compromise nutrient digestion and reduce growth performance in animals. The negative impact of AFB1 on the % change in average daily gain (ΔADG) of chickens and pigs was estimated based on meta-analysis: ΔADG (%)chicken = −0.13 × AFB1 intake per body weight (ng/g·d) and ΔADG (%)pig = −0.74 × AFB1 intake per body weight (µg/kg·d), indicating that increasing AFB1 contamination linearly reduces the growth of animals. To mitigate the harmful impacts of AFB1, various dietary strategies have been effective. Mycotoxin-detoxifying agents include mycotoxin-adsorbing agents, such as clay and yeast cell wall compounds, binding to AFB1 and mycotoxin-biotransforming agents, such as specific strains of Bacillus subtilis and mycotoxin-degrading enzyme, degrading AFB1 into non-toxic metabolites such as aflatoxin D1. Multiple mycotoxin-detoxifying agents are often combined and used together to improve the intestinal health and growth of chickens and pigs fed AFB1-contaminated feeds. In summary, AFB1 negatively impacts intestinal microbiota, induces immune responses and oxidative stress, disrupts intestinal morphology, and impairs nutrient digestion in the small intestine, leading to reduced growth performance. Supplementing multi-component mycotoxin-detoxifying agents in feeds could effectively adsorb and degrade AFB1 co-contaminated with other mycotoxins prior to its absorption in the small intestine, preventing its negative impacts on the intestinal health and growth performance of chickens and pigs.

Artemia Nauplii Enriched with Soybean Lecithin Enhances Growth Performance, Intestine Morphology, and Desiccation Stress Resistance in Yellow Drum (Nibea albiflora) Larvae

The inherent deficiency of phospholipids in Artemia limits its nutritional value as live prey for marine fish larvae. In our previous study, we optimized a phospholipid enrichment method by incubating Artemia nauplii with 10 g of soybean lecithin per m3 of seawater for 12 h, significantly enhancing their phospholipid content. Purpose: The present study evaluated the impact of this enrichment on yellow drum (Nibea albiflora) larvae, focusing on growth performance, intestinal morphology, body composition, weaning success, and desiccation stress resistance. Methods: The larvae (12 days post-hatching, dph) were fed either soybean lecithin-enriched (SL group) or newly hatched (NH group) Artemia nauplii for 10 days. Results: By the end of the experiment, the SL group exhibited a markedly greater body weight and standard length compared to the NH group (p < 0.05). This growth improvement was due to enhanced intestinal morphology, characterized by a significantly higher mucosal fold height, microvillus density, and microvillus length (p < 0.05). Intestinal RNA sequencing identified 160 upregulated and 447 downregulated differentially expressed genes (DEGs) in the SL group compared to the NH group. Soybean lecithin enrichment reduced the expression of lipogenesis-related genes (fasn, scd, elovl1) while upregulating lipid catabolism genes (ppara, cpt1, cpt2), indicating increased lipid breakdown and energy production. After a 5-day weaning period onto a commercial microdiet, the SL group continued to show significantly superior growth performance. In an afterward desiccation stress test, larvae from the SL group demonstrated significantly higher survival rates, potentially due to the decreased expression of intestinal cytokine genes (ccl13, mhc1, mhc2) observed in the RNA-seq analysis. Conclusion: This study highlights that feeding soybean lecithin-enriched Artemia nauplii enhances growth performance and desiccation stress in yellow drum larvae by promoting lipid catabolism, improving intestinal structure, and regulating immune responses.

Dietary rosemary oil with/without zymogen forte improves water quality, growth hormones, immune-physiological response, stress resilience, and health status of Chelon ramada grown in groundwater

With freshwater resources becoming scarce worldwide, mariculture is a promising avenue to sustain aquaculture development, especially by incorporating brackish and saline groundwater (GW) use into fish farming. A 75-day rearing trial was conducted to evaluate fish growth, immune response, overall health, and water quality of Chelon ramada cultured in brackish GW and fed on a basal diet (BD) augmented with rosemary oil (RO) or RO + zymogen forte™ (ZF) as an anti-flatulent. Five treatments were administrated in triplicate: T1: fish-fed BD without additives (control group); T2: fish-fed BD + 0.5 g RO /kg diet; T3: fish-fed BD + 0.5 g RO and 1 g ZF /kg diet; T4: fish-fed BD + 1 g RO /kg diet; T5: fish-fed BD + 1 g RO and 1 g ZF /kg diet. Three hundred fish (8.51 ± 0.01 g/fish) were housed in 15 fiberglass tanks (1500-L tank). The results revealed significant improvements (P < 0.05) in growth performance, survival, growth hormone, and insulin-like growth factor-1. Additionally, there were decreases in the feed conversion ratio (FCR) and the levels of nitrogen by-products (NH4, NH3, and NO2) and pathogenic bacterial counts in the rearing water when fish were fed diets supplemented with RO and RO + ZF. Furthermore, significant reductions in the levels of plasma stress indicators (cortisol, creatinine, and glucose) were detected. In addition, there were significant enhancements observed in the levels of innate immune markers, such as white blood cells, total protein, albumin, and immunoglobulin. The complement system, specifically complement 3 and complement 4, also showed considerable improvements. Furthermore, there were increases in plasma heat shock proteins HSP70 and HSP90, as well as enhanced antioxidant activity. These gains were associated with healthier liver and intestines. The investigation demonstrated that adding 0.5–1 g RO / kg diet or RO + ZF to a C. ramada diet has many benefits, including reducing the levels of nitrogen by-product chemicals and pathogenic bacterial load in GW used in growth tanks. Furthermore, significant improvements were observed in the rates of growth and associated hormones, efficiency of feed utilization, blood indicators, immune function, condition of internal organs (namely the intestine and liver), and overall health of the fish.

Fish protein hydrolysates from rainbow trout processing in replacement of feed protein sources: Effects on growth performances, liver status and body composition of gilthead sea bream, Sparus aurata L., juveniles

Fish protein hydrolysates from rainbow trout processing in replacement of feed protein sources: Effects on growth performances, liver status and body composition of gilthead sea bream, <i>Sparus aurata</i> L., juveniles

Nutrient use and methane emissions in growing beef fed different protein sources and a pasture-based diet.

This study investigated the effects of different protein sources on feed intake, nutrient, and energy utilization, growth performance, and enteric methane (CH4) emissions in growing beef cattle, also evaluated against a pasture-based diet. Thirty-two Holstein × Angus growing beef were allocated to four dietary treatments: a total mixed ration (TMR) including solvent-extracted soybean meal as the main protein source (SB; n = 8), TMR with local brewers' spent grains (BSG; n = 8), TMR with local field beans (BNS; n = 8), and a diet consisting solely of fresh-cut Italian ryegrass (GRA; n = 8). Every four weeks, animals were moved to digestibility stalls within respiration chambers to measure nutrient intakes, energy and nitrogen (N) utilization, and enteric CH4 emissions. Feed intake (Calan gates), nutrient intakes, and CH4 emissions (GreenFeed) were also measured when animals were group-housed. In respiratory chambers, enteric CH4 yield per kg of dry matter intake (DMI), per kg of organic matter intake (OMI), and per kg body weight were lower (P < 0.05) for GRA. Feces and urine energy outputs were higher (P = 0.007 and P < 0.001, respectively) for GRA steers than concentrate-fed steers. Urinary nitrogen output (UNO, P = 0.026), manure (feces+urine) nitrogen output (MNO, P = 0.034), UNO/nitrogen intake (P = 0.002), and MNO/nitrogen intake (P = 0.006) were higher for GRA. During group-housing periods, CH4 emissions, measured by GreenFeed, were similar to those measured in chambers. Similar CH4 yield between treatments, expressed per kg digestible DMI and digestible OMI, may indicate that the lower diet digestibility was likely the reason for the reduced enteric CH4 emissions in pasture-based diets. The higher energy output and nitrogen losses, and the reduced nitrogen utilization for steers fed the fresh-cut ryegrass diet indicate less efficient energy and nitrogen utilization, which can be considered environmentally undesirable. The lower growth rates in the pasture-based system should also be accounted for when this is adopted for reducing production costs.

Effects of dietary oregano essential oil supplementation on carcass traits, muscle fiber structure, oxidative stability, meat quality, and regulatory mechanisms in Holstein steers.

Dietary supplementation for beef cattle, using natural plant extracts, such as oregano essential oil (OEO), has proven effective in enhancing growth performance, beef production quantity and quality, and ensuring food safety. However, the precise mechanisms underlying these effects remain unclear. This study investigated the impact of OEO on carcass traits, muscle fiber structure, meat quality, oxidative status, flavor compounds, and gene regulatory mechanisms in the longissimus thoracis (LT) muscles of beef cattle. Eighteen steers were randomly assigned to two groups (n = 9 per group) and fed either a control diet (CK) or the same diet supplemented with 20 g of OEO per head per day for 300 days. Oregano essential oil supplementation improved the body weight, carcass weight, meat production, area and diameter of fiber, ether extract, and water-holding power of muscle. Increasing catalase (CAT), peroxidases (POD), glutathione peroxidase (GSH-Px) and by decreasing lipid droplets (LDs) reduced muscle lipid oxidation. However, the color (L*, a*, b*, C* and H°) and the flavor compounds of muscle were affected adversely by OEO. The transcriptome and metabolome indicated the OEO group enriched fat synthesis, proteo-metabolism, antioxidants, and growth significantly. Five key genes (SH2B2, CD209, LOC504773, C1QC, and HMOX) and ten downregulated metabolites (deoxyguanosine, d-melezitose, maltotriose, raffinose, melatonin, quinic acid, orotic acid, hydrocinnamic acid, 2-methylsuccinic acid, and pyridoxal 5'-phosphate) were identified as key biomarkers. These interacted to positively influence the growth, oxidative status, and meat quality of steers positively. These findings suggest that OEO, as a natural bioactive compound, can serve as an additive for beef cattle, with a daily dose of 20 g per steer improving growth and meat quality, although it may affect muscle color and flavor negatively. © 2025 Society of Chemical Industry.

Supplementation with Complex Phytonutrients Enhances Rumen Barrier Function and Growth Performance of Lambs by Regulating Rumen Microbiome and Metabolome

Complex phytonutrients (CPS) have attracted extensive interest due to their anti-inflammatory effects. This investigation focused on the impact of CPS on rumen health in lambs on high-concentrate diets, emphasizing growth performance, ruminal fermentation, epithelial barrier integrity, ruminal metabolism, and microbial communities. A total of 54 lambs, 3 months old and with a 30.42 ± 0.54 kg body weight, were randomly assigned to three treatment groups, with six replicates per treatment and three lambs per replicate. The lambs received a basal diet (the ratio of concentrate to forage was 75:25) without CPS supplementation (CON) or with the inclusion of 2.5 g/kg (CPS2.5) or 5.0 g/kg CPS (CPS5.0) for a total of 60 days. The CPS groups exhibited increased growth performance and improved rumen fermentation parameters. Mechanistically, CPS enhanced rumen epithelial barrier function, thereby lowering inflammation and inhibiting the overactivation of the JNK/p38 MAPK signaling pathway, and the effect of CPS5.0 was better than that of CPS2.5. Notably, CPS5.0 could optimize the composition of rumen microbiota and increase the levels of Ursolic acid and other metabolites. The strong associations between rumen bacteria and health-related indicators and differential metabolites were further highlighted. Our findings suggest that adding CPS to lambs’ diets has widespread positive impacts, including improved growth performance, reduced inflammation and mRNA relative expression of apoptosis-related genes, enhanced barrier function, and beneficial changes in the rumen microbiome and metabolite profiles.

Utilizing Invasive Pterygoplichthys pardalis as a Sustainable Fish Meal Substitute and Euphorbia hirta Extract Supplement: Effects on Growth Performance, Organosomatic Indices, Hematological Profiles, and Serum Biochemistry in Chinese Bullfrogs (Hoplobatrachus chinensis)

This research examined the efficacy of substituting commercial fish meal (CFM) with Pterygoplichthys pardalis meal (PPM) in Hoplobatrachus chinensis diets, with and without Euphorbia hirta extract (EHE) supplementation. The study utilized six dietary treatments: a control diet (0% PPM, no EHE) and five experimental diets with varying PPM levels (0%+, 25%+, 50%+, 75%+, and 100%+), each fortified with 300 mg/kg EHE. The experiment spanned 90 days. The analysis revealed that PPM exhibited superior amino acid profiles compared to CFM, both in quality and quantity, while CFM demonstrated higher fatty acid content. The growth metrics showed a significant decline only in the group receiving 100% PPM replacement with EHE supplementation. Most organosomatic indices remained consistent across the treatments, with the exception of intraperitoneal fat, which decreased in all EHE-supplemented groups. Blood parameters, including white blood cells, red blood cells, and hematocrit, along with serum proteins (total protein, globulin, and albumin), displayed an upward trend in all EHE-supplemented groups. The 50%+ and 75%+ PPM replacement groups exhibited significantly elevated serum glucose levels (p &lt; 0.05). Liver enzymes (alanine transaminase and aspartate transaminase) showed no significant variations among the treatments. The results indicate that PPM can serve as an effective replacement for up to 75% of CFM in H. chinensis feed, without compromising their growth performance. Moreover, supplementing with EHE helps to enhance essential biochemical indices in the body, without adversely affecting liver function. This investigation offers valuable perspectives on the development of sustainable aquaculture feed and the potential application of invasive fish species in aquatic animal nutrition.

Core microbe Bifidobacterium in the hindgut of calves improves the growth phenotype of young hosts by regulating microbial functions and host metabolism

BackgroundThe growth and health of young ruminants are regulated by their gut microbiome, which can have lifelong consequences. Compared with subjective grouping, phenotypic clustering might be a more comprehensive approach to revealing the relationship between calf growth state and core gut microbes. However, the identification of beneficial gut bacteria and its internal mechanisms of shaping host phenotype differentiation remains unclear.ResultsIn this study, calves were divided into two clusters, cluster1 and cluster2, based on 29 phenotypic indicators using cluster analysis. Calves in cluster2 showed better growth performance, including higher body weight (BW), average daily gain (ADG), and dry matter intake (DMI), as well as better serum indicators with a high level of total superoxide dismutase (T-SOD), interleukin-6 (IL-6), and insulin-like growth factor-1 (IGF-1) compared to those in cluster1. Multi-omics was used to detect microbial features among calves in different phenotypic clusters. Distinct differences were observed between the two clustered gut microbiomes, including microbial diversity and composition. The close relationships between growth performance, blood metabolites, and microbiome were also confirmed. In cluster2, Bifidobacterium members were the dominant contributors to microbial metabolic functions with a higher abundance. Furthermore, pathways involved in carbohydrate degradation, glycolysis, and biosynthesis of propionate and proteins were active, while methane production was inhibited. In addition, the diversity and richness of hindgut resistome in cluster2 were lower than those in cluster1. The isolation and culture of Bifidobacterium strain, as well as the mice experiment, indicated that B. longum 1109 from calf feces in cluster2 could promote the growth of young hosts, enhance their blood immunity and antioxidation, and improve the development of hindgut.ConclusionsIn summary, cluster analysis has proved to be a feasible and reliable approach for identifying phenotypic subgroups of calves, prompting further exploration of host-microbiome interactions. Bifidobacterium as a core microbe in the hindgut of calves may play a crucial probiotic role in host phenotypic differentiation. This study enhances our comprehension of how gut core microbe shapes the host phenotype and provides new insights into the manipulation of beneficial gut colonizers to improve the growth performance and productivity of young ruminants.EbRLLe7F-yvVZ5Dd-FAxmeVideo

Effects of Chinese Herbal Medicines on Growth Performance, Antioxidant Capacity, and Liver and Intestinal Health of Hybrid Snakehead (Channa maculata ♀ × Channa. argus ♂)

Chinese herbal medicines have become a new green feed additive in the aquaculture industry. The aim of this study is to investigate the effects of traditional Chinese herbal medicines (Isatidis radix, Forsythia suspensa, and Schisandra chinensis) on the growth performance, antioxidant capacity, and intestinal microbiota of hybrid snakehead (Channa maculata ♀ × Channa argus ♂). A total of 600 fish (mean weight: 15.85 ± 0.15 g) were randomly assigned to five groups, including the control group (CG), I. radix extract group (IRE), F. suspensa extract group (FSE), S. chinensis extract group (SCE), and the Chinese herbal medicine mixture group (CHMM; a mixture of extracts of I. radix, F. suspensa, and S. chinensis at the ratio of 1:1:1) for 6 weeks. The results show that the IRE-supplemented diet improved the survival rate (SR), feed efficiency ratio (FE), and condition factor (CF) compared to others. Compared to the control group, the activity of superoxide dismutase (SOD) in plasma and intestine was significantly increased in the FSE and CHMM groups, whereas the content of malondialdehyde (MDA) in plasma and liver was significantly reduced in the SCE group. A 16s rRNA analysis indicates that dietary supplementation with FSE significantly promoted the proliferation of Fusobacteriota, while IRE supplementation increased the alpha diversity of intestinal bacteria. In conclusion, the addition of I. radix to the diet of hybrid snakehead improves growth, antioxidant capacity, and liver and intestine health, and modulates the intestinal microbiota of snakehead positively.

Probiotic administration aggravates dextran sulfate sodium salt-induced inflammation and intestinal epithelium disruption in weaned pig

BackgroundA. muciniphila (AKK) has attracted extensive research interest as a potential next-generation probiotics, but its role in intestinal pathology is remains unclear. Herein, this study was conducted to investigate the effects of A. muciniphila DSM 22,959 on growth performance, intestinal barrier function, microecology and inflammatory response of weaned piglets stimulated by dextran sulfate sodium salt (DSS).MethodTwenty-four Duroc × Landrace × Yorkshire (DLY) weaned piglets used for a 2 × 2 factorial arrangement of treatments were divided into four groups with six piglets in each group. From 1 to 15 d, the CA and DA groups were orally fed with 1.0 × 1011 colony-forming units A. muciniphila per day, while the CON and DCON groups were received gastric infusion of anaerobic sterile saline per day. The pigs were orally challenged (DCON, DA) or not (CON, CA) with DSS from day 9 to the end of the experiment and slaughtered on day 16.ResultsPresence of A. muciniphila in DSS-challenged weaned pigs resulted in numerically increased diarrhea rate, blood neutrophilic granulocyte, serum C-reactive protein and immunoglobulin M levels, and numerically reduced final weight, average daily feed intake and average daily gain. The decrease in intestinal villus height, villous height: crypt depth ratio and digestibility was accompanied by lower expression of ZO1, ZO2, Claudin1, DMT1, CAT1, SGLT1 and PBD114 genes, as well as decreased enzyme activities of intestinal alkaline phosphatase, lactase, sucrase and maltase of piglets in DA group compared to piglets in DCON group. The abundance of Bifdobacterium, Lactobacillus, A. muciniphila, Ruminococcus gnavus was numerically higher in digesta of pigs in DA group than those in DCON group. The inflammatory responses of piglets were dramatically changed by the simultaneous presence of A. muciniphila and DSS: expression level of IL17A, IL17F, IL23, RORγt, Stat3 was elevated in DA pigs compared to the other pig groups.ConclusionsOur result showed that the oral A. muciniphila aggravates DSS-induced health damage of weaned piglet, which may attribute to the deteriorating intestinal morphology, dysbiosis of microbiota and inflammatory response disorders.

Latitudinal variation in leaf functional traits of an invasive salt-marsh plant and its relationship with growth performance

Latitudinal variation in leaf functional traits of an invasive salt-marsh plant and its relationship with growth performance

Multi-omics dataset of individual variations in growth performance of large yellow croaker

Large yellow croaker (Larimichthys crocea) is a highly economically important marine fish species in China. However, substantial individual variations in growth performance have emerged as a limiting factor for the sustainable development of the large yellow croaker industry. Gut microbiota plays a crucial role in fish growth and development by regulating metabolic processes. To explore these dynamics, we employed metagenomics, transcriptomics, and untargeted metabolomics to comprehensively analyze the structure of the intestinal microbiome and its relationship with intestinal metabolism and host gene expression. We constructed association models for “gut microbiota–differentially expressed genes”, “differentially expressed genes–metabolites,” and “gut microbiota–metabolites.” Sequencing data and LC–MS/MS raw data have been deposited in NCBI and MetaboLights databases for public access. Our findings offer critical insights into the molecular mechanisms underlying growth variations in L. crocea and provide valuable data for the selective breeding of improved strains.

Impact of Mating Methods and Semen Preservation on Reproductive and Growth Performances in Palestinian Assaf Sheep

As global demand for sheep products increases, improving reproductive efficiency and lamb growth performance has become a priority for sheep farmers. Artificial insemination (AI) offers several advantages over natural mating, including improved genetic selection and disease control. This study contributes to understanding the impact of different mating methods and semen preservation techniques on reproductive performance and growth traits in Palestinian Assaf sheep under local breeding conditions. The research included 123 adult Assaf ewes from two farms, which were hormonally synchronized and divided into three groups for different reproductive biotechnologies. Group 1 (G1) comprised 50 ewes inseminated with frozen semen straws, while Group 2 (G2) had 37 ewes inseminated with fresh semen from genetically superior rams. Both G1 and G2 used 0.5 mL straws containing 250 million sperm, with insemination occurring 48 h post sponge removal and eCG hormone injection. Group 3 (G3) consisted of 36 ewes that underwent natural mating at a ratio of 1 ram to 6 ewes. The study revealed that insemination methods significantly affected fertility rates (45.4, 61.1, and 71.9% for G1, G2, and G3, respectively; p &lt; 0.05). Artificial insemination notably enhanced lamb performance indicators (p &lt; 0.05), although it did not significantly influence prolific lambing (p &gt; 0.05). Prolific lambing significantly impacted birth weight, weaning weight, and average daily gain at 60 days across all groups (p &lt; 0.05), but not the average daily gain or final weight at 180 days (p &gt; 0.05). The management system significantly affected birth and weaning weights at 60 days (p &lt; 0.05), but not the final weight at 180 days or prolific lambing (p &gt; 0.05). Lamb sex significantly influenced average daily gains at 60 and 180 days, as well as final weight at 180 days (p &lt; 0.05). The findings suggest that, while artificial insemination with semen from elite rams may reduce fertility rates, it significantly improves lamb performance rates. In conclusion, our study demonstrates that, while natural mating offers higher fertility rates, AI with fresh semen can be a valuable tool for improving lamb growth performance in Palestinian Assaf sheep. These findings provide valuable insights for sheep farmers in the region to optimize reproductive strategies and enhance lamb production

Effects of Bile Acids on Growth Performance, Hepatopancreatic Antioxidant Capacity, Intestinal Immune-Related Gene Expression, and Gut Microbiota of Penaeus vannamei

This study aimed to examine the impact of varying concentrations of bile acids (BA) added to the feed on several aspects of Penaeus vannamei. The purity of BA was 25.29%, and its main components were 5.74% chenodeoxycholic acid, 6.27% allocholic acid, 3.20% cholic acid, 5.79% hyodeoxycholic acid, and 2.31% hyocholic acid. The experiment was designed with four groups: CT, BA1, BA2, and BA3, where BA were added to the shrimp basal diet at concentrations of 0.0 mg/kg, 0.1 mg/kg, 1.0 mg/kg, and 10.0 mg/kg, respectively. After 60 days of farming P. vannamei (initial body weight: 1.21 ± 0.05 g), the results showed that BA supplementation significantly improved growth performance, and BA2 group was the most significant, which increased the final weight (FBW) by 18.6%, weight gain rate (WGR) by 19.5%, and survival rate (SR) by 5.8% compared with the CT group (p &lt; 0.05). Additionally, the activities of trypsin and lipase in gut tissue were significantly increased (p &lt; 0.05). Furthermore, BA supplementation increased the activity of antioxidant-related enzymes in the hepatopancreas and enhanced the mRNA expression levels of gut-associated immune genes. In addition, the supplementation of 0.1 mg/kg BA significantly altered the gut microbial composition, reducing the proportion of harmful Proteobacteria while enhancing the relative abundance of beneficial microorganisms such as Firmicutes and Bacteroides. In conclusion, 1.0 mg/kg and 10.0 mg/kg BA supplementation significantly improved the growth performance, digestive capacity, and antioxidant capacity of shrimp, among which 1.0 mg/kg supplementation had the most significant effect and improved the intestinal microbial composition of shrimp.

Effect of Gardeniae Fructus Powder on Growth Performance, Antioxidant Capacity, Intestinal Barrier Function, and Colonic Microbiota of Weaned Piglets

The present study aimed to explore the effect of GF powder on the growth performance, diarrhea rate, antioxidant and immune capacity, and intestinal health of weaned piglets. A total of 144 weaned piglets (8.29 ± 0.11 kg) at 21 d old were randomly assigned to four groups, with each treatment consisting of six replicate pens, with six piglets per pen, and each pen containing three barrows and three gilts. The piglets were fed a basal diet supplement with 0%, 0.4%, 0.6%, and 0.8% GF powder (n = 36). Our results indicated that compared with the basal diet, the F/G and diarrhea rate were remarkably decreased in the 0.8% GF group (p &lt; 0.05). Serum biochemical parameters showed that supplementation with GF significantly increased the content of HDL-C (0.6 and 0.8% levels), IL-6 (0.8% level), IL-10 (0.4, 0.6, and 0.8% levels), Ig G (0.4% level), and Ig A (0.8% level) compared with the basal diet (p &lt; 0.05). The index of antioxidant capacity showed that compared with a basal diet, supplementation with GF significantly decreased serum MDA content (0.4% and 0.8% levels) and jejunal and ileal MDA content (0.4%, 0.6%, and 0.8% levels) (p &lt; 0.05). Additionally, compared with the basal diet, supplementation with GF significantly increased serum and ileal T-AOC content (0.4%, 0.6%, and 0.8% levels), serum T-SOD content (0.4% and 0.8% levels), ileal T-SOD content (0.4%, 0.6%, and 0.8% levels), CAT content (0.4%, 0.6%, and 0.8% levels), and jejunal GSH-Px content (0.8% level) (p &lt; 0.05). The results of gene expression indicate that compared with the basal diet, supplementation with GF significantly increased Nrf 2 (0.4% level), NQO (0.4% level), SOD 1 (0.4% and 0.8% levels), and GCLC (0.4% level) and GCLM (0.8% level) abundance in jejunal mucosa; supplementation with GF significantly increased Nrf 2 (0.4%, 0.6%, and 0.8% levels), HO-1 (0.4% level), NQO (0.8% level), SOD 1 (0.4% and 0.8% levels), and GCLC (0.4% level) and GCLM (0.8% level) abundance in ileal mucosa (p &lt; 0.05). Ulteriorly, the present results indicate that supplementation with GF at the 0.8% level significantly increased the villus height in the jejunum and ileum as well as the villus/crypt ratio in the ileum compared with the basal diet (p &lt; 0.05). Compared with the basal diet, 0.4% GF significantly increased Occludin gene expression in ileal mucosa (p &lt; 0.05), 0.6% GF significantly increased ZO-1, Claudin-1, and Occludin gene expression in jejunal mucosa (p &lt; 0.05), and 0.8% GF significantly increased ZO-1 and Occludin gene expression in jejunal mucosa along with Occludin expression in ileal mucosa (p &lt; 0.05). Furthermore, colonic microbiota composition showed that Shannon, observed species, and Chao 1 indices were significantly increased in the 0.8% GF group compared with the basal diet (p &lt; 0.05). At the phylum level, in comparison with the basal diet, the relative abundance of Firmicutes significantly decreased in the 0.4%, 0.6%, and 0.8% GF groups, and Bacteroidetes increased in the 0.8% GF group (p &lt; 0.05). At the genus level, compared with the basal diet, 0.6% and 0.8% GF significantly increased Prevotella abundance, and 0.6% GF significantly decreased Coprococcus abundance (p &lt; 0.05). At the species level, compared with the basal diet, 0.8% GF significantly increased Prevotella copri abundance, and 0.4%, 0.6%, and 0.8% GF significantly decreased Blautia obeum abundance (p &lt; 0.05). In summary, a dietary supplement with 0.8% Gardeniae Fructus powder significantly decreased the F/G and diarrhea rate and improved antioxidant capacity and intestinal barrier function, which may be associated with the improvement of the relative abundance of Prevotella copri. These findings indicate that Gardeniae Fructus powder may be used as a feed additive in swine weaning.

16S rRNA and metabolomics reveal the key microbes and key metabolites that regulate diarrhea in Holstein male calves

IntroductionDiarrhea is a prevalent disease among calves, which significantly hinders their growth and development, thereby impacting farm productivity and revenue. This study aimed to investigate the impact of diarrhea on calf growth.MethodsHolstein male calves with similar birth weight (39.5 ± 4.2 kg) were included in this study, and key parameters such as fecal score, diarrhea incidence, and growth performance from birth to weaning were measured. Rectal fecal samples from both diarrheic (n = 24) and healthy calves (n = 24) aged 1–4 weeks were analyzed using 16S rRNA gene sequencing and untargeted metabolomics.ResultsOur findings indicated a high prevalence of diarrhea among calves between 1–4 weeks of age on pasture, which led to a marked decrease in growth performance, including average daily gain. At the genus level, the relative abundance of GCA-900066575 in one-week-old diarrheic calves was significantly higher; Escherichia-Shigella and Pseudoflavonifractor were more abundant in two-week-old calves; while Tyzzerella and Lachnospiraceae_UCG-004 increased significantly in four-week-old calves, and correlated negatively with average daily gain, suggesting that these bacteria may promote the occurrence of diarrhea. Correlation analysis revealed that fecal metabolites such as arachidonic acid, cis-vaccenic acid, oleic acid, choline, creatinine, and others were significantly negatively correlated with calf growth performance and were significantly increased in diarrheic calves. WGNCA identified that dark magenta module metabolites were significantly associated with diarrhea traits from 1–4 weeks. Thirteen metabolites, including glycerophospholipids (such as 1-stearoyl-2-hydroxy-sn-glycero-3-phosphoethanolamine), fatty acids (such as dodecanoic acid), and arachidonic acid, were positively correlated with GCA-900066575, Escherichia-shigella, Tyzzerella, and Clostridium_butyricum, but negatively correlated with UBA1819, Lachnoclostridium_sp_YL32, and Clostridium_scindens.DiscussionTherefore, GCA-900066575, Escherichia-shigella, Lachnospiraceae_UCG-004, and Tyzzerella are likely key bacterial genera causing diarrhea in calves, while arachidonic acid, glycerol phospholipids, and fatty acids are critical metabolites associated with this condition. These alterations in the fecal microbiota and metabolite composition were found to be the principal contributors to growth retardation in diarrheic calves.

Comparative Study of Rapeseed Meal and Fermented Rapeseed Meal Replacing Soybean Meal in Diet on Growth Performance, Nutrient Digestibility and Economics in Broiler Chicken

Background: The escalating costs of soybean meal (SBM), coupled with environmental and socioeconomic considerations, necessitate exploration into alternative protein sources. Due to consistent increase in feed cost of SBM it was the need of hour to explore the alternatives for SBM. The present experiment was designed to evaluate the comparative study of rapeseed meal (RSM) and fermented rapeseed meal (FRSM) replacing soybean meal in diet on performance of broilers. Methods: Four hundred twenty, day old Vencobb-430Y straight run broiler chicks were randomly distributed into seven treatment groups with four replicates in each having fifteen chicks. The seven treatments were control group (T1), basal diet with 5% RSM (T2), basal diet with 10% RSM (T3), basal diet with 15% RSM (T4), basal diet with 5% FRSM (T)5, basal diet with 10% FRSM (T6), basal diet with 15% FRSM (T7). Result: The live body weight and cumulative weight gain at the end of 6th week significantly (P less than 0.01) decreased in T4 (15% RSM) and T7 (15% FRSM) as compared to control group T1. At 6th week, significantly (P less than 0.01) better cumulative feed conversion ratio was recorded in groups T1, T2, T3, T5 and T6 as compared to groups T4 and T7. There were non-significant differences for dry matter digestibility (%), nitrogen retention (%), ether extract digestibility (%) and crude fibre digestibility (%) in all groups. The highest net profit per kg live body weight was observed in treatment group T5 followed by groups T6, T3, T2, T1, T7 and T4. Thus, it was concluded that the inclusion of RSM and FRSM up to 10% level with replacing soybean meal do not have any adverse effect on growth performance, nutrient digestibility and improved economics of broiler production. Moreover, the inclusion of 5% FRSM with replacing soybean meal in diet has more economically profitable without affecting production performance in broilers.

The Growth Performance and Linear Body Measurements of Mongrel Rabbits in a Tropical Environment

The Growth Performance and Linear Body Measurements of Mongrel Rabbits in a Tropical Environment