This paper deals with the physical layer security for multi-unmanned aerial vehicle (UAV) assisted transmission of integrated sensing and communication (ISAC) signals from a dual-function base station (BS) to the ground user. Here, an active eavesdropper (Eve) on the ground eavesdrops on the information transmitted by the UAV network to the user. We propose a Stackelberg game model for the game problem of information security transmission between UAV and Eve, with perfect or imperfect channel state information (CSI) and cost information. To deal with the Stackelberg game problem, the dual optimization theory and KKT conditions are used to solve it, then we prove the existence and uniqueness of Stackelberg equilibrium (SE). At the same time, considering that the influence of ISAC signal’s sensing of Eve’s location on the distance between Eve and the user, and thus on the utility function of both sides of the game, this paper uses the Cramer–Rao bound (CRB) to represent the accurate performance of location sensing. Then the relaxation method is used for the non-convex optimization problem, and convex optimization tools can be used subsequently. Finally we notice that both operations have their own optimal resolution strategy, it is difficult to reach agreement, therefore an overall algorithm is proposed to solve the whole problem iteratively. In the simulation results, the effect of power on game and location sensing, and the effect of the distance between Eve and the user on the utility function of both sides of the game are given. In addition, we give the relationship between the CRB threshold and the transmit power, and verify the convergence of the game and overall algorithm.