Perennial Research Articles

Characterization of the complete chloroplast genome of Rhodiola sachalinensis and comparative analysis with its congeneric plants.

Rhodiola, belonging to the Crassulaceae family, is a perennial herbaceous plant genus. There are about 90 Rhodiola species worldwide, some of which have been reported to have medicinal properties. Rhodiola sachalinensis is a perennial medicinal herb within this genus and, in the present study, its chloroplast genome was sequenced, assembled, annotated and compared with 24 other Rhodiola species. The results obtained show that the chloroplast genome of R. sachalinensis is 151 595 bp long and has a CG content of 37.7%. The inverted repeats (IR) region of the Rhodiola chloroplast genome is the most conserved region, with the main differences being observed in the ycf1 and ndhF genes at the IRb-small single copy boundary, and rps19 and trnH genes at the IRa-large single copy boundary. Phylogenetic analysis showed that Rhodiola species form two major clades, and species with recorded medicinal properties, clustered together in one branch except for R. dumulosa. Within the genus, R. sachalinensis is most closely related to Rhodiola rosea, although comparative analyses showed that only R. sachalinensis and Rhodiola subopposita contained the psbZ gene, which encodes a highly conserved protein subunit of the Photosystem II core complex. Overall, the present study contributes to the understanding of the chloroplast genome of Rhodiola species, and provides a theoretical basis for the study of their genetic diversity and possible use as medicinal plants.

Clonal integration facilitates the expansion of Hydrocotyle vulgaris from a limited space to a larger area

AbstractClonal integration is an important ecological advantage of clonal plants. To ask whether clonal integration can help invasive plants escape space limitations, we tested the hypothesis that it can promote the growth of apical ramets when their connected basal ramets grow in limited space. We conducted a greenhouse experiment on the common perennial herb Hydrocotyle vulgaris. Clonal fragments consisting of pairs of connected ramets grew with basal ramets in three different sizes of pots (small, medium, and large) and apical ramets in large pots, and the connection between ramets was either severed or left intact. Pot size significantly affected the growth of basal ramets such that the biomass, number of leaves and flowers, and stolon length were in general greater in medium pots than in large and small pots when stolons were intact and were greater in medium and large pots than in small pots when stolons were severed. Furthermore, pot size interacted with severance to affect the performance of H. vulgaris. When the basal ramets grew in small pots, the intact stolon resulted in a significant promotion of apical ramet growth, but such positive effect was not found when the basal ramets grew in medium and large pots. Our results suggest that H. vulgaris is able to promote the growth of apical ramets to occupy the surrounding areas through clonal integration when the space where basal ramets grow is limited.

Panax notoginseng: panoramagram of phytochemical and pharmacological properties, biosynthesis, and regulation and production of ginsenosides

Abstract Panax notoginseng is a famous perennial herb widely used as materials of medicine and health-care food. Due to its various therapeutic effects, research work on P. notoginseng rapidly increased in recent years, urging a comprehensive review of the research progress of this important medicinal plant. Here, we summarized the latest studies on the representative bioactive constituents of P. notoginseng and their multiple pharmacological effects like cardiovascular protection, anti-tumor, and immunomodulatory activities. More importantly, we emphasized the biosynthesis and regulation of ginsenosides, which is the main bioactive ingredients of P. notoginseng. Key enzymes and transcription factors (TFs) involved in the biosynthesis of ginsenosides were reviewed, including diverse CYP450s, UGTs, bHLH, and ERF TFs. We also constructed a transcriptional regulatory network based on multi-omics data and predicted candidate transcription factors mediating the biosynthesis of ginsenosides. Finally, the current three major biotechnological approaches for ginsenoside production were highlighted. This review covered advances in the past decades, providing insights into quality evaluation and perspectives for the rational utilization and development of P. notoginseng resources. Modern omics technologies facilitate the exploration of the molecular mechanisms of ginsenoside biosynthesis, which is crucial to the breeding of novel P. notoginseng varieties. The identification of functional enzymes for biosynthesizing ginsenosides will lead to the formulation of potential strategies for the efficient and large-scale production of specific ginsenosides.

Patterns of constitutive and induced herbivore defence are complex, but share a common genetic basis in annual and perennial monkeyflower

Abstract Despite multiple ecological and evolutionary hypotheses that predict patterns of phenotypic relationships between plant growth, reproduction and constitutive and/or induced resistance to herbivores, these hypotheses do not make any predictions about the underlying molecular genetic mechanisms that mediate these relationships. We investigated how divergent plant life‐history strategies in the yellow monkeyflower and a life‐history altering locus, DIV1, influence plasticity of phytochemical herbivory resistance traits in response to attack by two herbivore species with different diet breadth. Life‐history strategy (annual vs. perennial) and the DIV1 locus significantly influenced levels of constitutive herbivory resistance, as well as resistance induction following both generalist and specialist herbivory. Perennial plants had higher total levels of univariate constitutive and induced defence than annuals, regardless of herbivore type. Annuals induced less in response to generalist herbivory than did perennials, while induction response was equivalent across the ecotypes for specialist herbivory. The effects of the DIV1 locus on levels of constitutive and induced defence were dependent on genetic background, the annual versus perennial haplotype of DIV1 and herbivore identity. The patterns of univariate induction due to DIV1 were non‐additive and did not always match expectations based on patterns of divergence for annual/perennial parents. For example, perennial plants had higher levels of constitutive and induced defence than did annuals, but when the annual DIV1 was present in the perennial genetic background induction response to herbivory was higher than for the perennial parent lines. Patterns for multivariate defence arsenals generally echoed those of univariate, with annual and perennial monkeyflowers and those with alternative versions of DIV1 differing significantly in constitutive and induced resistance. Like univariate resistance, induced multivariate defence arsenals were affected by herbivore identity. Our results highlight the complexity of the genetic mechanisms underlying plastic response to herbivory. While a genetic locus underlying substantial phenotypic variation in life‐history strategy and constitutive defence also influences defence plasticity, the induction response also depends on genetic background. This result demonstrates the potential for some degree of evolutionary independence between constitutive and induced defence, or induced defence and life‐history strategy, in monkeyflowers. Read the free Plain Language Summary for this article on the Journal blog.

Anthropogenic shrub encroachment has accelerated the degradation of desert steppe soil over the past four decades

Anthropogenic and natural shrub encroachment have similar ecological consequences on native grassland ecosystems. In fact, there is an accelerating trend toward anthropogenic shrub encroachment, as opposed to the century-long process of natural shrub encroachment. However, the soil quality during the transition of anthropogenic shrub encroachment into grasslands remains insufficiently understood. Here, we used a soil quality assessment method that utilized three datasets and two scoring methods to evaluate changes in soil quality during the anthropogenic transition from temperate desert grassland to shrubland. Our findings demonstrated that the soil quality index decreased with increasing shrub cover, from 0.49 in the desert grassland to 0.31 in the shrubland. Our final results revealed a gradual and significant decline of 36.73 % in soil quality during the transition from desert grassland to shrubland. Reduced soil moisture levels, nutrient availability, and microbial activity characterized this decline. Nearly four decades of anthropogenic shrub encroachment have exacerbated soil drought conditions while leading to a decrease in perennial herbaceous plants and an increase in bare ground cover; these factors can explain the observed decline in soil quality. These findings emphasize the importance of considering soil moisture availability and potential thresholds when implementing revegetation strategies in arid and semiarid regions.

Anthriscus sylvestris: An overview on Bioactive Compounds and Anticancer Mechanisms from a Traditional Medicinal Plant to Modern Investigation.

Anthriscus sylvestris (L.) Hoffm. Gen. is a biennial or perennial herb commonly found in China. It has a long history of use in traditional Chinese medicine to treat various ailments such as cough, gastric disorders, spleen deficiency, and limb weakness. Recently, its potential as an anticancer agent has gained considerable attention and has been the subject of extensive research focusing on extract efficacy, identification of active compounds, and proposed molecular mechanisms. Nevertheless, further high-quality research is still required to fully evaluate its potential as an anticancer drug. This review aims to comprehensively summarize the anticancer properties exhibited by the active components found in Anthriscus sylvestris. We conducted a comprehensive search, collation, and analysis of published articles on anticancer activity and active compounds of A. sylvestris using various databases that include, but are not limited to, PubMed, Web of Science, Science Direct and Google Scholar. The primary chemical composition of A. sylvestris consists of phenylpropanoids, flavonoids, steroids, fatty acids, and organic acids, showcasing an array of pharmacological activities like anticancer, antioxidant, anti-aging, and immunoregulatory properties. Thus, this review highlights the active compounds isolated from A. sylvestris extracts, which provide potential leads for the development of novel anticancer drugs and a better understanding of the plant's pharmacological effects, particularly its anticancer mechanism of action.

Salt stress alters the cell wall components and structure in Miscanthus sinensis stems.

Miscanthus is a perennial grass suitable for the production of lignocellulosic biomass on marginal lands. The effects of salt stress on Miscanthus cell wall composition and its consequences on biomass quality have nonetheless received relatively little attention. In this study, we investigated how exposure to moderate (100 mM NaCl) or severe (200 mM NaCl) saline growing conditions altered the composition of both primary and secondary cell wall components in the stems of 15 Miscanthus sinensis genotypes. The exposure to stress drastically impacted biomass yield and cell wall composition in terms of content and structural features. In general, the observed compositional changes were more pronounced under severe stress conditions and were more apparent in genotypes with a higher sensitivity towards stress. Besides a severely reduced cellulose content, salt stress led to increased pectin content, presumably in the form of highly branched rhamnogalacturonan type I. Although salt stress had a limited effect on the total lignin content, the acid-soluble lignin content was strongly increased in the most sensitive genotypes. This effect was also reflected in substantially altered lignin structures and led to a markedly reduced incorporation of syringyl subunits and p-coumaric acid moieties. Interestingly, plants that were allowed a recovery period after stress ultimately had a reduced lignin content compared to those continuously grown under control conditions. In addition, the salt stress-induced cell wall alterations contributed to an improved enzymatic saccharification efficiency.

Study of the influence of the extract of pipsissewa on cell cultures

The development of new diuretics of plant origin is an actual direction. Chimaphila umbellata (L.) is a perennial herb with diuretic, astringent, analgesic and other effects; and it can treat various conditions such as edema, dropsy, etc. Pipsissewa herb helps the removal of nitrogenous and chloride salts from the body due to the content of arbutin glycoside, tannins (up to 5 %). The aim. Evaluation of the effect of pipsissewa extract on L929 cell culture. Materials and methods. Cell line L929 (fibroblasts of mouse adipose tissue) was obtained in the low-temperature bank of the Institute of Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine. Cells were cultured in DMEM medium (Bio West, France) enriched with 10 % FBS (Lonza, Germany) with 1 % antibiotic-antimycotic (Bio West, France), in a CO2 incubator (Thermo Fisher Scientific, USA) at 37 ºС in an atmosphere with 5 % CO2. Determination of the minimum toxic concentration at which the cells remained alive was evaluated by morphological features (shape, monolayer integrity, adhesion to plastic). The study of the effect of pipsissewa extract on various cell functions was determined by the following methods: the ability to preserve morphological integrity - by the phase-contrast microscopy method, energy exchange - by the MTT test method, pinocytotic function - by the neutral red absorption method, migratory function - by the scratch test method, proliferative activity - by the doubling calculation method population Results. It is proposed to use concentrations of 0.05, 0.02, 0.01, 0.005 % of pipsissewa extract for further research. After carrying out the MTT reaction, the transition of MTT to formazan was confirmed microscopically in the negative control (native cells), at PE concentrations of 0.01 % and below, and the absence of a reaction in the positive control (cells killed by ethanol) at PE concentrations above 0.02 %. When recording the parameters of the NP absorption reaction, it was determined that PE at a concentration of 0.02 % and higher sharply suppresses pinocytotic activity, despite the partial preservation of cell adhesion, reducing the concentration by two times no longer affects mitochondria. A concentration of 0.01 % reduces proliferative activity, and at a concentration of 0.005 %, no difference with the control values ​​was found. Conclusions. When studying the assessment of the effect of pipsisewa extract on L929 cell culture, a toxic effect on these cells was established when added to the culture medium at a concentration above 0.01 %. The toxic effect had a threshold effect. Migratory and proliferative functions were the most sensitive, energy, pinocytosis and preservation of morphological integrity of cells were less sensitive

Study of the influence of the extract of pipsissewa on cell cultures

The development of new diuretics of plant origin is an actual direction. Chimaphila umbellata (L.) is a perennial herb with diuretic, astringent, analgesic and other effects; and it can treat various conditions such as edema, dropsy, etc. Pipsissewa herb helps the removal of nitrogenous and chloride salts from the body due to the content of arbutin glycoside, tannins (up to 5 %). The aim. Evaluation of the effect of pipsissewa extract on L929 cell culture. Materials and methods. Cell line L929 (fibroblasts of mouse adipose tissue) was obtained in the low-temperature bank of the Institute of Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine. Cells were cultured in DMEM medium (Bio West, France) enriched with 10 % FBS (Lonza, Germany) with 1 % antibiotic-antimycotic (Bio West, France), in a CO2 incubator (Thermo Fisher Scientific, USA) at 37 ºС in an atmosphere with 5 % CO2. Determination of the minimum toxic concentration at which the cells remained alive was evaluated by morphological features (shape, monolayer integrity, adhesion to plastic). The study of the effect of pipsissewa extract on various cell functions was determined by the following methods: the ability to preserve morphological integrity - by the phase-contrast microscopy method, energy exchange - by the MTT test method, pinocytotic function - by the neutral red absorption method, migratory function - by the scratch test method, proliferative activity - by the doubling calculation method population Results. It is proposed to use concentrations of 0.05, 0.02, 0.01, 0.005 % of pipsissewa extract for further research. After carrying out the MTT reaction, the transition of MTT to formazan was confirmed microscopically in the negative control (native cells), at PE concentrations of 0.01 % and below, and the absence of a reaction in the positive control (cells killed by ethanol) at PE concentrations above 0.02 %. When recording the parameters of the NP absorption reaction, it was determined that PE at a concentration of 0.02 % and higher sharply suppresses pinocytotic activity, despite the partial preservation of cell adhesion, reducing the concentration by two times no longer affects mitochondria. A concentration of 0.01 % reduces proliferative activity, and at a concentration of 0.005 %, no difference with the control values ​​was found. Conclusions. When studying the assessment of the effect of pipsisewa extract on L929 cell culture, a toxic effect on these cells was established when added to the culture medium at a concentration above 0.01 %. The toxic effect had a threshold effect. Migratory and proliferative functions were the most sensitive, energy, pinocytosis and preservation of morphological integrity of cells were less sensitive

Production Cost of Biocarbon and Biocomposite, and Their Prospects in Sustainable Biobased Industries

This study evaluated the economic prospects of biocarbon and biocomposite in the automotive industry and bioeconomy. The production cost of biocarbon produced from Miscanthus (a perennial grass), biocarbon-reinforced polypropylene (PP) composite (hereafter referred to as biocomposite), and automotive components are determined. The production cost of biocomposite was compared with inorganic filler-reinforced polymer composite (a conventional composite, i.e., talc reinforced PP composite). The production cost of biocarbon and biocomposite is estimated to be $513.1/ton and between $3536.7–$3647.3/ton, respectively (all dollar figures are in Canadian dollars). On the other hand, the cost of the conventional composite is likely to be $3544.8/ton. However, the production cost of an automotive component can be reduced by 9–11% compared with the conventional component if the components are produced from biocomposite. Further, this study determined the net present values (NPV) of a biocarbon plant and a composite manufacturing plant. The NPV of a biocarbon plant ($42.9 million) and a composite manufacturing plant ($34.0–$34.8 million for biocomposite and $34.7 million for conventional composite) showed that both the biocarbon and composite manufacturing phases are economically attractive. We concluded that by taking an industrial symbiosis approach, the biocomposite industry can be financially more attractive and contribute more to the bioeconomy.

Voluntary agricultural insurance in the Republic of Kazakhstan based on the example of perennial plantings

The goal is to assess the potential of voluntary agricultural insurance in Kazakhstan using the example of perennial crops, determine demand and market readiness, and identify difficulties faced by agricultural producers and insurance companies. Methods - to solve the problems, content analysis method was used, which is aimed at studying problems related to state support of agro-industrial complex, data, statistical indicators were considered as information base, as well as review of literature on the topic, legislative acts and state policy in the field of agriculture and agricultural insurance, experience of economically developed countries were studied. The results show the possibility of the presence of agricultural risks and uncertainties with focus on insurance of perennial crops, prospects and barriers that impede its development in conditions of sharply continental climate, scarcity of water resources and unstable economy. Conclusions - voluntary insurance protection is an effective tool for mitigating financial losses in agricultural production in Kazakhstan. The author notes the need to increase financial resources and strengthen consulting work focused on understanding the benefits of agricultural insurance by business entities; creating effective mechanisms for distributing insurance payments and developing affordable insurance products that take into account the needs of the farmers. The most flexible insurance conditions, minimum list of documents for concluding an agreement, transparent methods for establishing the amount of insurance compensation and settling losses, increasing investment attractiveness, and using international experience taking into account the needs of Kazakhstani agricultural producers are required. The role of insurance services as one of the most common methods of reducing farmers' losses from natural and climatic changes, will increase.

Advancing the dominance of winter annuals under changing rainfall patterns in a temperate desert of Central Asia

Understanding how climate change-induced alterations in rainfall patterns impact the composition and structure of plant diversity is crucial. Examining variations in plant life forms is vital for comprehending ecosystem dynamics. This study analyzes plant life form diversity, rainfall variability, and the impact of different rainfall patterns on plant diversity over 15 years in the Gurbantünggüt Desert, a temperate desert in Central Asia. The study identified 96 plant species in the area, comprising 27 winter annuals and 33 perennial herbs. Over the past 15 years, species diversity has remained stable, while diversity among plant life forms has varied significantly. Winter annuals consistently dominated, showing a significant linear increase in importance values (slope=1.135). Conversely, perennial shrubs like Haloxylon ammodendron and Haloxylon persicum exhibited a marked declining trend. Over the past 15 years, the occurrence of minor (<1 mm) and moderate (1–5 mm) rainfall during the spring and autumn seasons initially rose and subsequently fell. Concurrently, heavy rainfall events (≥5 mm) in spring have waned, showing notable yearly fluctuations. The frequency of heavy autumnal downpours has remained steady, with consistent interannual variations. Minor rainfall events primarily influenced winter annuals and perennial herbs during the spring and autumn, whereas heavy rainfall mainly affected perennial shrubs during these seasons. Our investigations suggest that winter annuals, despite their expanding presence, are ineffective in their ecological functions such as dune stabilization and reducing aeolian erosion in the peak summer months. In contrast, perennial shrubs like H. ammodendron and H. persicum, crucial for these roles, are markedly declining. The variable plant life forms might lead to modifications in the ecological roles of the region, thereby rendering it imperative to persistently observe this desert and proactively formulate conservation strategies for the vegetation.

Study of physical and mechanical properties of plant fruits on the example of walnut

Perennial plantations that are zoned in Ukraine allow to obtain high-quality fruits (nuts), which are widely used in the perfumery, pharmaceutical, oil, confectionery and other industries, as well as in the production of feed and feed additives. The known technologies of mass processing of walnut kernels mostly work well with crops that have a spherical or close to spherical external shape. The simplest, but least productive, are primitive manual devices for peeling walnut shells. Despite the simplicity of their design, these devices are inefficient and unsuitable for adjusting their operation to different varieties and physical and mechanical properties of biological fruits. The analysis of the known designs of walnut shell destruction machines and mechanisms showed the need to study the physical and mechanical properties of biological fruits (walnuts), to develop technical recommendations for the design of impact mechanisms to improve the quality of shell destruction and the possibility of their application in mass production technologies. A research methodology was developed, a laboratory setup was manufactured, and experiments were conducted. To determine the strength characteristics, the author designed and manufactured a device. It consists of a lever mechanism and a deforming device. The dimensions and load measurements were carried out according to well-known methods of physical mechanics. The statistical analysis of the results showed the need for in-depth scientific research of the geometric parameters of the design of the working bodies of machines for destroying the walnut shell in the mechanisms of fruit peeling machines, taking into account the quality indicators performance of works with certain conditions of their implementation. We conclude that according to the results of the experiments, there is a fairly high dependence between the geometric dimensions of the walnut fruit at the level of 0.72, 0.57 and 0.49, respectively. The weight of the fruit also depends on its dimensional characteristics, which is logical at the level of 0.6–0.75. The force of critical static fracture has a weak correlation with the dimensional characteristics at the level of 0.3. And it depends little on the plane of its application.

Effect of Harvest Time on Growth and Bioactive Compounds in Salvia miltiorrhiza.

Danshen (Salvia miltiorrhiza) is a perennial medicinal plant belonging to the Lamiaceae family. It is adapted to a wide range of soil pH with the potential to serve as an alternative crop in the United States. To enhance its cultivation and economic viability, it is crucial to develop production practices that maximize bioactive compound yields for danshen. The objective of this study was to investigate the effects of different harvest times on plant growth and subsequent yields of bioactive components of danshen. Three harvest times were selected (60, 120, or 180 days after transplanting [DAT]). In general, plants harvested at 180 DAT had higher plant growth index (PGI), shoot number, shoot weight, root number, maximum root length, maximum root diameter, and root weight compared to plants harvested at 60 or 120 DAT. However, plants harvested at 60 or 120 DAT had higher SPAD (Soil Plant Analysis Development) values. Plants harvested at 120 or 180 DAT had a higher content of tanshinone I, tanshinone IIA, cryptotanshinone, and salvianolic acid B compared to those harvested at 60 DAT. This study provides insights for optimizing the time of harvest of danshen to maximize plant growth and bioactive compound production.

Functional validation of AaCaM3 response to high temperature stress in Amorphophallus albus

Amorphophallus is a perennial monocotyledonous herbaceous plant native to the southwestern region of China, widely used in various fields such as food processing, biomedicine and chemical agriculture. However, Amorphophallus is a typical thermolabile plant, and the continuous high temperature in summer have seriously affected the growth, development and economic yield of Amorphophallus in recent years. Calmodulin (CaM), a Ca2+ sensor ubiquitous in eukaryotes, is the most important multifunctional receptor protein in plant cells, which affects plant stress resistance by participating in the activities of a variety of signaling molecules. In this study, the key gene AaCaM3 for the Ca2+-CaM regulatory pathway was obtained from A. albus, the sequence analysis confirmed that it is a typical calmodulin. The qRT-PCR results demonstrated that with the passage of heat treatment time, the expression of AaCaM3 was significantly upregulated in A. albus leaves. Subcellular localization analysis revealed that AaCaM3 localized on the cytoplasm and nucleus. Meanwhile, heterologous transformation experiments have shown that AaCaM3 can significantly improve the heat tolerance of Arabidopsis under heat stress. The promoter region of AaCaM3 was sequenced 1,338 bp by FPNI-PCR and GUS staining assay showed that the promoter of AaCaM3 was a high-temperature inducible promoter. Yeast one-hybrid analysis and Luciferase activity reporting system analysis showed that the AaCaM3 promoter may interact with AaHSFA1, AaHSFA2c, AaHSP70, AaDREB2a and AaDREB2b. In conclusion, this study provides new ideas for further improving the signal transduction network of high-temperature stress in Amorphophallus.

Biodiversity of plants in suburban meadows to create urban lawns with wildflowers

The creation of natural lawns is a means of optimizing costs for maintaining green infrastructure in the city by including natural biodiversity in lawns, which will increase the stability of plant communities in lawns and the quality of ecosystem services. Purpose. Determine the predominant species of plant groups of natural phytocenoses, which are further appropriate for planting in semi-natural lawns to ensure the stability of territories.. Methods. The Ramensky method was employed to determine the quantitative composition of natural lawns in the study areas, while the Raunkiær classification was utilized to identify persistent and perennial plant species. Results. The geobotanical investigation enabled the identification of dominant species in the study areas, providing insights into the phytocoenological composition of natural lawns for the city of Kyiv. This served as the foundation for developing recommendations for a plant catalogue tailored to the creation of such lawns in Kyiv. The grass stand at Lysa Hora exhibited the highest stability, with a significant presence of native species. Adventitious species were less dominant in this area, likely due to their conditional pathogenicity. Conversely, Trukhaniv Island displayed dominance by typical species, albeit with the occasional presence of azonal species. Mount Shchekavytsia showed competition between azonal and typical species. Surveys of recreational areas, including Lysa Hora, Mount Shchekavytsia, and Trukhaniv Island, underscored the presence of native, azonal, and adventive species. The study identified common native hemicryptophytes, including filamentous fescue, grey fescue, and creeping wheatgrass. A developed plant catalogue is poised to enhance ecosystem regulation services, such as microclimate regulation, water and wind erosion control, biodiversity maintenance, and social services through increased territorial attractiveness. Conclusions. When creating a natural lawn, the best species are considered to be indigenous, which allows for the sustainability of plant communities. The study identified the following native species that will successfully complement lawn coverings in the city: gray and filamentous fescue, ground fescue, creeping thyme, green strawberry, field carnation, and oak sage. The recommendations can be applied in other cities of Ukraine with similar physical and geographical conditions.

Прогноз развития процессов деградации на эрозионно опасных склонах черноземов обыкновенных Ростовской области

The decline in soil fertility in recent decades has led to a number of problems in agriculture. The spread of degradation processes forces the use of modern methods of modeling and forecasting erosion. The purpose of this work was to make a long-term forecast of the degradation processes of ordinary chernozems on sloping lands in crop rotations of various designs and using various agricultural technologies using automated neural networks. The research was conducted in a multifactorial long-term experiment, which is located on the slope of the Bolshoy Log beam, in 1990-2022. The experience was laid down in 1986 in the system of contour and landscape organization of the slope area with a steepness of up to 3.5-4°. In the experiment, crop rotations with a different ratio of the proportion of pure steam and perennial grasses in the structure of crops, as well as two tillage systems, were studied. As a result of the research, a forecast was made for a hundred years ahead using automated neural networks, which revealed a general trend of attenuation of erosion processes on slopes, but with varying degrees of intensity, both in crop rotations of various designs and when using various agricultural technologies for cultivating crops. Cultivation of crops in crop rotations with a different proportion from 20 to 40 % of perennial grasses in the structure of sown areas reduces the runoff of meltwater and stormwater by 22.9-24.9 %, washing away the soil by 26.4-28.6 %, and in some cases completely, under exist-ing conditions. The use of soil protection treatment will reduce the runoff of meltwater and stormwater by 39.2-72.0 %, and flushing by 41.5 %.

Comprehensive Review of Cyclamen: Development, Bioactive Properties, and Therapeutic Applications

Plants are being researched as potential sources of novel drugs, which has led to a recent acceleration in the discovery of new bioactive compounds. Research on tissue culture technology for the synthesis and processing of plant compounds has skyrocketed, surpassing all expectations. These plants can be bought either raw or as extracts, where some of the chemicals are extracted by mashing the plant in water, alcohol, or another solvent. The use of herbal medicine may open new chances for reducing the onset of infections and treating different diseases including cancer. A perennial plant that blooms in the winter, Cyclamen, is one of the most widely used potted flowers in many nations. Alkaloids, flavonoids, phenols, tannins, saponins, sterols, and glycosides are the main active components of Cyclamen. Analgesic, cytotoxic, antioxidant, antimicrobial, and anti-inflammatory properties have all been demonstrated as potential effects of various extracts of Cyclamen tubers. However, the use of this medicinal plant in official medicine will require further research in the areas of pharmacology. Furthermore, it is necessary to create standard operating procedures for a crude herbal medication. In this regard, this review aims to highlight the key characteristics of the Cyclamen plant, such as its various parts, species, stages of development, and geographic range; pinpoint its intriguing bioactivities, its antioxidant, anti-inflammatory, and its anti-cancerous effects; and ascertain its potential medicinal uses and the main future perspectives.

Transcriptome analysis revealed the role of moderate exogenous methyl jasmonate treatments in enhancing the metabolic pathway of L-borneol in the Blumea balsamifera.

Blumea balsamifera L. (Ainaxiang) DC. is a perennial herb of the compositae family. It is also the primary source of natural borneol. Endo-borneol, the principal medical active element in B. balsamifera, is anti-inflammatory, antioxidant, and analgesic; enhances medicine absorption; refreshes; and is used as a spice and in cosmetic. Industrialization of B. balsamifera is limited by its low L-borneol concentration. Thus, understanding the accumulation pattern of the secondary metabolite endo-borneol and its synthesis process in secondary metabolism is critical for increasing B. balsamifera active ingredient content and cultivation efficiency. In this work, B. balsamifera was treated with varying concentrations (1.00 and 10.00 mmol/L) of methyl jasmonate (MeJA) as an exogenous foliar activator. The physiological parameters and L-borneol concentration were then assessed. Transcriptome sequencing of B. balsamifera-induced leaves was used to identify key genes for monoterpene synthesis. The treatment effect of 1 mmol/L MeJA was the best, and the leaves of all three leaf positions accumulated the highest L-borneol after 120h, correspondingly 3.043 mg·g-1 FW, 3.346 mg·g-1 FW, and 2.044 mg·g-1 FW, with significant differences from the control. The main assembly produced 509,285 transcripts with min and max lengths of 201 and 23,172, respectively. DEG analysis employing volcano blots revealed 593, 224, 612, 2,405, 1,353, and 921 upregulated genes and 4, 123, 573, 1,745, 766, and 763 downregulated genes in the treatments D1_1vsCK, D1_10vsCK, D2_1vsCK, D2_10vsCK, D5_1vsCK, and D5_10vsCK. Interestingly, when exposed to MeJA treatments, the MEP pathway's unigenes express themselves more than those of the MVA route. Finally, when treated with 1 mmol/L, the genes DXR, DXS, and GPS showed increased expression over time. At the same time, a 10 mmol/L therapy resulted in elevated levels of ispH and GGPS. Our preliminary research indicates that exogenous phytohormones can raise the level of L borneol in B. balsamifera (L.) DC when given in the appropriate amounts. The most significant discovery made while analyzing the effects of different hormones and concentrations on B. balsamifera (L.) DC was the effect of 1 mmol/L MeJA treatment.

The polyphyletic Caucasus-centred Campanula subg. Scapiflorae (Campanulaceae) revisited with a newly circumscribed C. sect. Tridentatae for its core clade.

Campanula L. is among the genera with the highest number of endemics in the Caucasus ecoregion. A group of attractive alpine and subalpine perennial rosette plants with short single-flowered stems centred in the Caucasus has been treated as Campanulasubg.Scapiflorae or at other ranks, with considerably varying circumscription and classification. Molecular phylogenetic analysis of three plastid DNA regions (trnK/matK, petD, rpl16) of a strongly extended sampling, comprising 23 of the 27 commonly accepted taxa (85%) with 330 accessions built on and guided by the results of our previous study of the group, confirmed the polyphyly of C.subg.Scapiflorae in any of its circumscriptions. The core clade of the group comprises exclusively endemics and near-endemics of the Caucasus and is treated here as C.sect.Tridentatae in a revised circumscription. The phylogenetic relationships of the disparate other elements of the Scapiflorae group are outlined.