Background: This study focuses on the development and validation of the Diversity, Equity, Inclusion, and Antiracism Staff Perceptions of College Climate (DEIA SPCC) Scale. The goal of this study was to create and validate a standardised tool to assess pharmacy staff perceptions of DEIA within their institutions, thereby aiding in the evaluation and advancement of DEIA initiatives in colleges of pharmacy. Methods: A 20-item survey covering four domains - diversity, equity, inclusion, and antiracism - was distributed to pharmacy staff through the American Association of Colleges of Pharmacy (AACP) directory. The survey's validity was assessed using factor analysis and reliability analysis. Results: There was a total of 135 staff member responses. Results demonstrated high reliability and consistency across all four domains, with the Cronbach’s alpha ranging between 0.764 to 0.951. Conclusion: The study concluded that the DEIA SPCC survey is a practical, validated tool for pharmacy institutions to assess and enhance their DEIA climate from the perspective of their staff, potentially extendable to other healthcare academic disciplines. Further research is suggested to validate the survey in broader contexts and explore the predictors of survey scores.