The study focused on the perception and experiences of internship supervisors in tertiary institutions, specifically, University of Education, Winneba (UEW), regarding internship supervision training they served as participants. Using a descriptive quantitative survey, a sample of 179 out of 315 faculty members were conveniently sampled for the study. The perception and experiences of respondents were measured using an adapted scale developed by De Vellis (2012). The data collected were analyzed descriptively and inferentially. The study revealed that internship supervisors had positive perception and experiences about internship supervision training. However, their perception and experiences were not influenced by their gender. Therefore, it was concluded that internship supervisors had positive perception and experiences about the need for internship supervision training. It was recommended that tertiary institutions in Ghana, specifically, UEW which is mandated to provide schools with quality professional teachers, make internship supervision training a core component of selecting supervisors to assess interns during internship so that the best and most qualified faculty member can be used for this vital activity.