Aim. People with attractive facial appearance are more easily integrated in society and are considered to be more capable, more intelligent and more responsible. The aesthetic aspect is influenced by expression, by facial symmetry, facial contours and teeth colour. In dental restorations, the correct determination of teeth colour is a critical step with extreme importance for successful treatment. From this perspective the practitioner must have experience, must have the ability to distinguish colours and must train through repetitive visual practical exercises that can have an impact on the perception of dental aesthetics. Materials and method. The study followed comparatively the evolution of teeth colour determination and its impact on dental aesthetics perception. It was conducted on a group of fifth year students from the Faculty of Dental Medicine, Bucharest using the Tooth Training Box colour determination exercise that is based on the 3-D Master colour key principle. Results. Using training exercises to determine colour participants developed a better understanding of the physical principles behind the procedure but also a significant increase in accuracy of their measurements and confidence in their colour determinations. Conclusions. Using an aesthetic evaluation protocol that translates teeth colour in a universal language for both practitioners as well as dental technicians has a significant impact in aesthetic dentistry.