Percentage Of Area Research Articles

Characterization, Bioactive Profiling and Evaluation of the Pharmacological Activities of Ethanol Extract of Lannea welwitschii (Hiern) Engl.

Introduction: Lannea welwitschii is a medicinal plant used in traditional medicine to treat various infectious diseases due to its plethora of bioactive compounds that possess various pharmacological activities. This study aimed to characterize and determine the phytochemical profile and the bioactive compounds responsible for their pharmacological activities. Methods: Phytochemical screening and Gas-Chromatography Mass Spectrometry (GC-MS) analysis were carried out on the ethanolic extract of L. welwitschii to determine the volatile compounds present. Characterization of the crude extract was carried out using Fourier Transform Infrared spectroscopy (FTIR), Scanning Electron Microscopy (SEM), X-ray diffraction (XRD) and Energy Dispersive X-ray spectroscopy (EDX). The antioxidant potential of the extract was evaluated using methods such as 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric-reducing antioxidant Potential (FRAP). The antibacterial activities were determined using the macro-broth dilution technique. Results: Secondary metabolites such as saponins, tannins, alkaloids, reducing sugars, and cardiac glycoside were identified. The GC-MS analysis showed the presence of 48 bioactive compounds, out of which 12 had peak area percentages ≥ 1%. Some of the bioactive compounds identified include 2-butoxy-ethanol, Dodecanoic acid, Stigmasterol, Isopropenyl, and n-hexadecanoic acid, which have been reported with different biological activities. FTIR analysis revealed the presence of various functional groups which showed major compounds in the plant extract. The morphological features showed the spherical shape of the plant extract with several aggregates, while the EDX analysis identified elements such as silicon, oxygen, and silver. The extract demonstrated inhibitory potential against some of the bacterial isolates. However, Bacillus pumilus, Klebsiella quasipneumoniae, Klebsiella aerogenes, and Staphylococcus aureus are more susceptible with MIC of 0.313 and 0.625 mg/ml respectively. Conclusion: The presence of secondary metabolites and bioactive compounds revealed by the characterization and phytochemical analysis provided enough evidence for the usage of the plant in the treatment of infectious diseases and thus might be considered a therapeutic agent.

Thornthwaite Moisture Index for the Triângulo Mineiro, Brazilian Cerrado Region, Under Climate Change

Climate change represents one of the main challenges of the 21st century for planning and sustainable development. However, little is known about how climate change can affect a region's climate zones. The objective was to evaluate probable changes in climatic zones using the Thornthwaite climate classification (1948). Historical series between 1981 and 2021 of rainfall and air temperature were used. The water balance was calculated from Thornthwaite and Mather. Thornthwaite's humidity index (1948) was used to classify localities according to their level of humidity and the scenarios RCP 2.6, RCP 4.5, RCP 6.0 and RCP 8.5 to analyze projections for the 21st century (period 2081–2100). The current characterization, with historical data, of the region's climate presented air temperature, rainfall and average potential evapotranspiration, respectively, of 22.4ºC, 1,318.8 mm and 1,123.74 mm, in addition to a water surplus of 391.04 mm and water deficit of 195.04 mm. The region currently has five climate indices, with a prevalence of more humid classes (B1, B2 and B3), corresponding to 62% of the territory. The results derived from the projections indicate reductions in climate classes and an increase in the area occupied by drier climates. For example, the percentage of area occupied by class C1 (dry subhumid) would increase from the current 8.4% to 69.68% in the RCP 8.5 scenario. The study of these change projections is important since profound consequences for the hydrology, ecology and social area of the region will take place, potentially harming agriculture, the region's main economic activity.

Country economic status is strongly associated with burn survival - validation of the (modified) ABSI

BackgroundPredicting burn-related mortality is vital for family counseling, triage, and resource allocation. Several of the burn-specific mortality prediction scores have been developed, including the Abbreviated Burn Severity Index (ABSI) in 1982. However, these scores are not tested for accuracy to support contemporary estimates of the global burden of burn injury. This study compares burn mortality across countries with varying economic levels, as indicated by the Human Development Index (HDI), to assess if a modified ABSI (mABSI) offers enhanced predictive accuracy.MethodsA retrospective study was performed, including over 90,000 patients from seven sources in five continents, including two burn registries and five referral burn centers. Data from 2015 to 2019 were collected, including age, gender, presence of inhalation injury, full-thickness burn, percentage of total body surface area, and outcome. The participating countries were classified based on the HDI and mortality was predicted using both the original and the modified ABSI.ResultsAfter removal of incomplete data, 74,460 sets remained for the analysis. Significant variations in population demographics, sample sizes, total body surface area (TBSA), and age distributions across the studied regions were noted. The modified ABSI demonstrated a smaller deviation from the 95% CI of the true survival probability than the original ABSI in countries with a very high or high HDI, indicating improved accuracy here. In these countries, the original ABSI overestimates mortality. Conversely, in countries with a middle or low HDI, the original ABSI remains more accurate, reflecting the significantly greater burn related mortality in countries with a low HDI.ConclusionIn burn patients, the probability of survival remains highly dependent on the level of development of a country. Our results support the use of the modified ABSI in countries with a (very) high HDI, yet in resource constraint settings, the original ABSI seems to provide more accurate predictions. These findings underscore the need for adaptive models that take into account socioeconomic variables, potentially guiding health policy adjustments and emphasizing the necessity of resource allocation and training in lower HDI countries. Such adaptations could enhance clinical outcomes and reduce disparities in burn care effectiveness globally.

Application of Machine Learning to Identify Influential Factors for Fecal Contamination of Shallow Groundwater

Understanding influential factors for fecal contamination in groundwater is critical for ensuring water safety and public health. The objective of this study is to identify key factors for fecal contamination of shallow tubewells using machine learning methods. Three methods, including recursive feature elimination (RFE) with XGBoost, Random Forest, and mutual information, were implemented to examine E. coli presence and concentration in 1495 tubewell water samples in Matlab, Bangladesh. For E. coli presence, climatic variables, including average rainfall and temperature over the 30, 15, and 7 days preceding sampling, as well as ambient temperature and rainfall on the sampling day, emerged as critical predictors. Land cover characteristics, such as the percentages of urban and agricultural areas within 100 m of a tubewell, were also significant. For E. coli concentration, land cover characteristics within 100 m, the number of hot and heavy-rain days in the 30 days preceding sampling, average rainfall and temperature in the 3 days preceding sampling, and ambient temperature on the sampling day were identified as key drivers. Random Forest and mutual information yielded results that were more similar to each other than to those of RFE with XGBoost. The findings highlight the interplay between climatic factors, land use, and population density in determining fecal contamination in shallow well water and demonstrate the power of machine learning algorithms in ranking these factors.

Protective mechanism of safflower yellow injection on myocardial ischemia-reperfusion injury in rats by activating NLRP3 inflammasome

ObjectivesThis study intended to explore whether the protective effect safflower yellow injection (SYI) on myocardial ischemia-reperfusion (I/R) injury in rats mediated of the NLRP3 inflammasome signaling.MethodsThe I/R model was prepared by ligating the left anterior descending coronary artery for 45 min and then releasing the blood flow for 150 min. 96 male Wistar rats were randomly divided into sham group, I/R group, Hebeishuang group (HBS), SYI high-dose group (I/R + SYI-H), SYI medium-dose group (I/R + SYI-M) and SYI low-dose group (I/R + SYI-L). Cell experiments were divided into normal control group (NC), Oxygen glucose deprivation/reoxygenation group (OGD/R), OGD/R + SYI group, OGD/R + SYI + Chloroquine group (OGD/R + SYI + CQ). The area of myocardial ischemia infarction and pathological changes were observed by the Tetrazolium method (TTC) and HE staining. Myocardial enzymes such as aspartate aminotransferase (AST), lactate dehydrogenase (LDH) and creatine kinase (CK) were measured by chemiluminescence (CL) method. The inflammatory factors levels of TNF-α, IL-1β, MCP-1, and IL-6 were detected by ELISA. The expressions of inflammatory-related proteins (Caspase-1, NLRP3, TLR4, NF-κB), autophagosome-related proteins (LC3-I, LC3-II,LC3-II/LC3-I), apoptosis-related proteins (Bax, Bcl-2, Caspase-3, Bcl-2/Bax) and autophagy-related proteins (p62/SQSTM1, PI3K, p-Akt, mTOR) were detected by Western-Blot. Cell morphology and cell viability were detected by transmission electron microscopy and CCK-8.ResultsIn vivo, compared with sham group, the percentage of myocardial infarction area was increased and myocardial tissue arrangement was disordered in I/R group. In addition, the activities of myocardial enzymes, the contents of inflammatory factors, the expressions of inflammatory-related proteins, autophagy-related proteins, autophagosome-related proteins, Bax and Caspase-3 were increased, while Bcl-2 and Bcl-2/Bax were decreased. SYI treatment reversed these trends, except for the expression of autophagosome-related proteins. In vitro, SYI decreased the contents of inflammatory factors and the expressions of inflammatory-related proteins, autophagy-related proteins and autophagosome-related proteins caused by OGD/R. However, the contents of inflammatory factors and the expression of inflammatory-related proteins, p62/SQSTM1 and mTOR were increased, while PI3K, p-AKT, LC3-II/LC3-I were significantly decreased in OGD/R + SYI + CQ group.ConclusionsSYI can promote myocardial tissue autophagy by regulating NLRP3, thereby attenuating the myocardial inflammatory response and protecting damaged myocardium in I/R rats.

Influencing Mechanism of Precipitation Pollution Intensity in Qinhuai River Basin Based on Random Forest

Analyzing the distribution characteristics of precipitation pollution intensity in the basin and identifying the main factors affecting the precipitation pollution intensity are the important basis for realizing the accurate management of diffused pollution. Based on the surface water quality data from four typical sections of the main stream of Qinhuai River Basin and rainfall data collected from 2021 to 2022, the distribution characteristics of precipitation pollution intensity in the basin were analyzed, and representative natural and social factors were selected to construct models of the precipitation pollution intensity of ammonia nitrogen （NH4+-N）, permanganate index, and total phosphorus （TP） based on random forest algorithm. Additionally, the main driving factors of precipitation pollution intensity were identified, and the influencing mechanism was analyzed. The results showed that rainfall was the main driving factor for the water quality exceeding the standard in the Qinhuai River Basin, and the proportion of rainfall events within 3 days of when the water quality of the sections in the study area exceeded the standard was between 61.4% and 97.4%. The precipitation pollution intensity of NH4+-N and TP showed a gradual increase from upstream to downstream. NH4+-N was the primary pollutant in the midstream Qiqiaoweng section and the downstream Sanchahekou section, with the precipitation pollution intensity ranging from 0.12 to 2.98 and from 0.31 to 3.84, respectively. The precipitation pollution intensity of permanganate index in the upstream Yangqiao section was the largest, ranging from 0.41 to 1.35, and the precipitation pollution intensity of the Jiangjundadao section, which is located on the channel of diverted water, was at a lower level compared with that of the other three sections. Water diversion was an important influencing factor for the reduction of precipitation pollution intensity of NH4+-N and permanganate index （P<0.01）； the proportion of built-up area was significantly positively correlated with the precipitation pollution intensity of NH4+-N and TP； the proportion of cultivated area was significantly positively correlated with the precipitation pollution intensity of permanganate index, and the proportion of forest land area was significantly negatively correlated with the precipitation pollution intensity of these three indicators. Under the conditions of light rain, moderate rain, and heavy rain, the rainfall was significantly positively correlated with the precipitation pollution intensity of each indicator, and the precipitation pollution intensity remained high under the condition of torrential rain. The post-rain pollution of NH4+-N and TP mainly originated from the middle and lower reaches of the study area, which was significantly affected by regional characteristics such as the percentage of built-up area and water diversion （P<0.01）, and the post-rain pollution of permanganate index mainly originated from the upstream area with a wide distribution of cultivated land area and was significantly affected by flow （P<0.01）.

Comparison of Synovialization and Clinical Outcomes between Anterior Cruciate Ligament Anteromedial Bundle Augmentation and Double-Bundle Anterior Cruciate Ligament Reconstruction.

This study aimed to compare clinical outcomes and second-look arthroscopic findings between double-bundle anterior cruciate ligament (ACL) reconstruction for complete ACL rupture and anteromedial (AM) bundle augmentation with preservation of the posterolateral (PL) bundle in isolated AM bundle rupture. Between 2014 and 2021, we retrospectively analyzed 95 ACL reconstructions with at least 2 years of follow-up. Patients undergoing primary ACL reconstruction from January 2014 to June 2021 were included in the study. Double-bundle ACL reconstruction (group 1) was performed in the cases involving male patients, high activity levels, age below 40 years, or when there was a patient preference for double-bundle reconstruction. In contrast, for partial ACL ruptures with an intact PL bundle confirmed on preoperative magnetic resonance imaging, AM augmentation was performed (group 2). Preoperative and final follow-up clinical outcomes, along with synovial coverage during second-look arthroscopy, were compared between group 1 (65 cases) and group 2 (30 cases). Statistical analysis included correlation coefficients between the groups. The Lysholm score, Tegner activity scores, and International Knee Documentation Committee (IKDC) knee evaluation form score at final follow-up were 81.8 (±5.5), 7.2 (±0.9), and 79.4 (±6.7), respectively, in group 1, and they were 88.0 (±6.4, p = 0.038), 7.9 (±0.5, p = 0.042), and 86.2 (±5.4, p = 0.081), respectively, in group 2. For group 1, the Lachman test (p = 0.093) and the pivot shift test (p = 0.176) at the final follow-up showed no laxity in 57 and 59 knees, and there was plus one laxity in 8 and 6 knees. For group 2, there were no laxity in 27 and 28 knees, and plus one laxity in 3 and 2 knees. Synovial coverage area percentages of the grafted tendon were 74.3 (±10.7) and 86.9 (±7.3, p = 0.002) in groups and group 2, respectively, with superior synovial coverage observed in group 2. The AM bundle augmentation with preservation of the PL bundle in ACL injury yielded better synovial coverage and clinical scores compared with double-bundle ACL reconstruction for complete ACL rupture.

Phytochemical profile and in vitro evaluation of cassava (Manihot esculenta Crantz) foliage as ruminant feed with/without green banana flour

Cassava (Manihot esculenta Crantz) is a crucial crop in tropics and subtropics, primarily cultivated for its tuber. However, its foliage is rich in protein and can supply essential elements for ruminants. The objective of this study was to evaluate the phytochemical compounds by Gas chromatography-MS (GC-MS) and the main phenolic by High Pressure Liquid Chromatography (HPLC) present in cassava foliage, along with the fermentation pattern using a semi-automated gas production (GP) system. The in vitro evaluation was carried out for four diets formulated as follows: T1 (alfalfa: grass hay at ratio of 30: 70); T2 (alfalfa: grass hay: banana flour 30:60:10); T3 and T4 (replaced alfalfa in T1 and T2 with cassava foliage, respectively). The addition of green banana flour aimed to increase the diets’ energy. The GC-MS results indicated that cassava foliage showed a large number of valuable bioactive components, with the biflavonoid isoginkgetin representing the major component at 25.33% of total peak area percentage. The HPLC analysis declared that rutin, gallic acid, and ferulic acid were the main phenolic compounds presented in cassava foliage ethanolic extract. The accumulative gas after 24 h of incubation was significantly lower with cassava diets compared to alfalfa diets, being 119.3 versus 130.1 ml/g DM, respectively. The degradation of both organic matter and neutral detergent fiber was significantly higher with alfalfa compared to cassava diets, while there was no significant difference between alfalfa and cassava diets on final pH, ammonia concentration and protozoal count. Banana flour inclusion, regardless of the forage type, decreased the accumulative gas after 24 h of incubation with about 9% compared with no banana addition. The use of cassava foliage in ruminant diets considered a promising protein source with valuable bioactive components that could have a positive effect on animal health and production.

New Approach in the Interpretation of Complex Triple-negative Breast Cancer Immunohistochemistry Specimens Processed With VENTANA PD-L1 (SP142) Assay.

Triple-negative breast cancer (TNBC) is challenging to treat because of its lack of specific molecular targets. The IMMUNOPEG study aimed to evaluate a novel structured method for interpreting TNBC immunohistochemistry specimens processed with VENTANA PD-L1 (SP142) assay. The study involved 10 pathologists who evaluated 50 different immunohistochemistry specimens of TNBC with programmed death ligand 1 (PD-L1) expression considered challenging and that were previously evaluated by the scientific committee, using the NAVIFY Digital Pathology platform. Initially, the overall percent agreement (OPA) was 74%, with a negative percent agreement (NPA) of 68.2% for samples classified as negative, and a positive percent agreement (PPA) of 94.5% for positive samples. After training on the method, the OPA improved significantly to 81.6%, with the NPA increasing to 80.5% and the PPA decreasing to 85.5%. The mean percentage of the tumor area occupied by PD-L1-stained immune cells decreased from 2.5% to 1.6% post-training, approaching to the scientific committee's consensus of 1.029%. The study found that the pathologists' confidence in their assessments increased significantly when using the structured method, which was found to be easy to use by 9 out of 10 pathologists. All pathologists agreed that the structured method was useful for assessing PD-L1 expression. The study suggests that this method has potential value in interpreting challenging cases of PD-L1 immunohistochemistry (IHC) in TNBC. Further refinement and a training protocol may be necessary to enhance the method's efficiency. The potential for generalizing this structured method to other IHC procedures and pathologies warrants additional research.

The Effects of Nicotine on Re-endothelialization, Inflammation, and Neoatherosclerosis After Drug-Eluting Stent Implantation in a Porcine Model.

Cigarette smoking is a major risk factor for atherosclerosis. Nicotine, a crucial constituent of tobacco, contributes to atherosclerosis development and progression. However, evidence of the association between nicotine and neointima formation is limited. We aimed to evaluate whether nicotine enhances neointimal hyperplasia in the native epicardial coronary arteries of pigs after percutaneous coronary intervention (PCI) with drug-eluting stents (DES). After coronary angiography (CAG) and quantitative coronary angiography (QCA), we implanted 20 DES into 20 pigs allocated to 2 groups: no-nicotine (n=10) and nicotine (n=10) groups. Post-PCI CAG and QCA were performed immediately. Follow-up CAG, QCA, optical coherence tomography (OCT), and histopathological analyses were performed 2 months post-PCI. Despite intergroup similarities in the baseline QCA findings, OCT analysis showed that the nicotine group had a smaller mean stent and lumen areas, a larger mean neointimal area, greater percent area stenosis, and higher peri-strut fibrin and inflammation scores than the no-nicotine group. In immunofluorescence analysis, the nicotine group displayed higher expression of CD68 and α-smooth muscle actin but lower CD31 expression than the no-nicotine group. Nicotine inhibited re-endothelialization and promoted inflammation and NIH after PCI with DES in a porcine model.

Association of Insulin Resistance With Radiographic Lung Abnormalities and Incident Lung Disease: The Framingham Offspring Study.

Insulin resistance (IR) may be a risk factor for lung disease, but objective evidence is limited. We sought to define the relationship of longitudinal IR with radiographic imaging outcomes and examiner-identified incident lung disease in the Framingham Offspring Study. Participants without baseline lung disease underwent repeated measurements of fasting insulin and glucose levels over an average period of 13.6 years, from which time-weighted average HOMA-IR was calculated. Each participant then underwent a cardiac gated whole-lung computed tomography scan, which was analyzed for the presence of emphysema, interstitial lung abnormalities (ILAs), and quantitative airway features. Incident lung disease was determined by a study examiner. The relationship of HOMA-IR to these outcomes was estimated in models adjusted for demographics, BMI, and lifetime smoking. A total of 875 participants with longitudinal IR data and outcomes were identified. Their mean age was 51.5 years, and BMI was 26.7 kg/m2. HOMA-IR was temporally unstable, with a within-person SD approximately two-thirds of the between-person SD. In adjusted models, a 1 SD increase in log(HOMA-IR) z score was associated with higher odds of qualitative emphysema (odds ratio [OR] 1.33; 95% CI 1.04-1.70), ILAs (OR 1.35; 95% CI 1.05-1.74), and modest increases in airway wall thickness and wall area percentage. These radiographic findings were corroborated by a positive association of HOMA-IR with incident lung disease. IR is associated with radiographic lung abnormalities and incident lung disease. Deeper phenotyping is necessary to define mechanisms of IR-associated lung injury.

Identifying priority conservation areas for threatened amphibian habitats in the Yangtze river Delta region to maintain regional biodiversity.

The complex life cycle traits of amphibians make them especially sensitive to environmental change, and their ongoing conservation requires the maintenance of suitable habitat that accounts for such life cycle characteristics which may impacted by local environmental dynamics arising from climate change and human disturbance. Many existing studies on amphibian habitats disregard this important issue, leading to uncertainty in managing critical habitats. The application of appropriate conservation practices is therefore constrained by the fact that the major factors influencing amphibian habitats, and their spatio-temporal dynamics at different life stages, are poorly understood. In responding to this notable shortcoming, we applied a Bayesian Belief Network model and geographical detector method to identify spatial and seasonal changes in threatened amphibian habitats of the Yangtze River Delta region (YRDR). Our results reveal the relative importance of different environmental factors contributing to spatio-temporal change of threatened amphibian habitats in YRDR. The results highlight the spatially heterogenous nature of amphibian habitats and the impacts of urbanization, as evidenced by quantitative metrics of percentage area of built-up land and distance to built-up land. Habitat conservation priority analyses also indicate the importance of prioritizing habitat restoration efforts in severely degraded areas. Analysis of seasonality indicates that amphibians face particularly severe challenges during their autumn hibernation period (Slope=-0.002). In revealing seasonal variations in the level of threat to amphibians at different life stages across different habitats, this study fills an important knowledge gap and therefore supports regional amphibian habitat conservation and restoration efforts. The results of the study further imply that current conservation strategies need to comprehensively account for seasonal changes, life-cycle stages, and spatial heterogeneity.

Comparison of Anteroposterior Vs. Axial X-rays for Assessing Bone Loss in Recurrent Shoulder Dislocation

Background: Recurrent shoulder dislocation frequently causes the glenoid and humeral head to gradually lose bone, which decreases joint stability and raises the risk of another dislocation. Treating bone loss accurately is crucial, especially when it comes to surgical procedures. The diagnostic precision of axial and anteroposterior (AP) X-rays in identifying and measuring bone loss in patients with repeated shoulder dislocations was examined in this study. Methods: 30 individuals who experienced recurrent shoulder dislocations had axial and anteroposterior (AP) radiography. The humeral head bone loss was measured in millimeters, whereas the glenoid bone loss was expressed as a percentage of the total glenoid surface area. The images were evaluated by two separate radiologists who determined the images' sensitivity, specificity, and accuracy for both modalities. The Intraclass Correlation Coefficient (ICC) was utilized to assess inter-observer reliability, and Receiver Operating Characteristic (ROC) curve analysis was employed to assess overall diagnostic performance. Results: The sensitivity of axial X-rays was found to be much higher (85%) than that of AP X-rays (70%; p < 0.05). Additionally, axial X-rays demonstrated higher accuracy (83% vs.68%) and specificity (80% vs. 65%). Using Axial X-rays, the mean glenoid bone loss was 16.5%, whereas using AP X-rays, it was 14.2%. For axial X-rays, the humeral head bone loss was 5.6 mm, but for AP X-rays, it was 4.8 mm. Compared to AP X-rays, the ICC for inter-observer reliability was greater for axial X-rays (0.88 and 0.85). Axial X-rays performed better diagnostically than AP X-rays (AUC = 0.71), according to ROC analysis. However, Gold standard investigation for bone loss in recurrent sholder dislocation is always a CT scan. Conclusion: When it comes to identifying and measuring bone loss in repeated shoulder dislocations, axial X-rays provide better diagnostic accuracy than AP X-rays, which is why they are the preferred imaging modality for preoperative evaluation.

THE CITY AND ITS SPATIAL TRANSFORMATIONS: A DIACHRONIC AND QUANTITATIVE ANALYSIS OF THE URBAN SPRAWL PROCESS. CASE STUDY OF TÉBESSA (ALGERIA)

Urban sprawl is a significant phenomenon affecting many cities, including the city of Tebessa in Algeria. This spatial dynamic, characterized by often uncontrolled expansion of the urban fabric towards the peripheries, generates profound transformations in the morphology and functioning of cities. This article proposes an in-depth analysis of this process through a methodology combining diachronic analysis and spatial quantitative analysis. The objective is to model the percentage of built-up area as a function of distance from the historic core, using a logistic function. This approach highlights the transition between the compact urban fabric of the center and the dispersed periphery, thus offering a measure of urban dilution. The study reveals that Tebessa has undergone significant spatial transformation since independence, transitioning from a compact urban morphology to a more extensive urban sprawl. The results demonstrate a gradual decrease in building density radiating outward from the center, illustrating the phenomenon of spatial dilution. Although urban expansion continues, its pace tends to stabilize. This research underscores the utility of the logistic model for understanding and forecasting urban growth dynamics, offering a new perspective on the spatial evolution of cities.

Diagnostic accuracy of pancreatic fibrosis grading with artificial intelligence: a single-center experience

Background: Chronic pancreatitis is a long-term fibro-inflammatory disease of the pancreas that worsens the quality of life of patients and is associated with life-threatening complications. Chronic pancreatitis is based on fibro-inflammatory changes in the tissue of the pancreas. The necessity of a more accurate identification of early fibrosis is related to discrepancies in assessments done with conventional visual scales. Aim: To evaluate the diagnostic accuracy of existing scales for visual assessment of the degree of pancreatic fibrosis compared to the techniques of whole-slide images (WSI) digital analysis. Methods: The morphometric analysis of the assessment of pancreatic fibrosis grade was carried out in the postoperative material from 118 patients with the use of two assessment semi-quantitative visual scales (those proposed by G. Klöppel B. Maillet in 1991 and by O. V. Paklina et al. in 2011), as well as with the use of the digital image marking in the ASAP software and an artificial intelligence algorithm in the QuPath software. Both intralobular and perilobular fibrosis grades were assessed, as well as their integrative index (II). Results: The assessment of pancreatic fibrosis with a 12-point rating system (II) according to Klöppel Maillett showed the following distribution: low grade fibrosis (LF), 55.9% of the cases, moderate fibrosis (MF), 25.4%, severe fibrosis (SF), 16.1%, no fibrosis (NF), 2.5% of the cases. According to the Paklina et al. scale, the results were as follows: LF, 66.1%, MF, 15.2%, SF, 12.7%, and NF, 5.9% of the cases. The comparison of the classical systems revealed a mismatch in the fibrosis grade in 27.9% of the cases; this resulted in the use of more objective methods for the WSI digital analysis. When comparing the average percentage of fibrosis area detected with ASAP with the fibrosis level according to Klöppel Maillett, the data were distributed as follows: with LF, the mean fibrosis area by ASAP was 6.9%, with MF, the fibrosis area was 30%, and with SF, 65%. Similarly, when counting cells in QuPath the following values were obtained: LF, 8.2%, MF, 27.9%, SF, 69.3%. The use of the WSI digital image analysis showed a higher concordance between them (R = 0.937) relative to the comparison between the classical rating scales (R = 0.811). When comparing the methods for assessment of fibrosis for the accurate diagnosis of chronic pancreatitis with ROC analysis, the best result was shown by the cell counting method with QuPath (AUC = 0.943). The method of area calculation with ASAP showed the AUC of 0.910, the classical Klöppel Maillett method demonstrated the AUC of 0.879 and the assessment according to Paklina et al. showed the AUC of 0.808. Conclusion: the WSI digital analysis has higher and significant concordance and diagnostic accuracy; therefore it can be used in clinical practice.

Enhanced Osseointegration of Titanium Alloy Bone Implants Coated With Carob-calcium Hydroxide Nanoparticles: A Comparative Study.

This study aimed to evaluate the osseointegration properties of titanium bone implants coated with carob-mediated calcium hydroxide nanoparticles biomechanically, radiographically, and histologically on rabbit tibias. Forty coated and forty uncoated titanium alloy bone implants were inserted into 20 New Zealand rabbits; each tibia received 2 implants. The rabbits were sacrificed after 4 or 8 weeks, and samples were retrieved for biomechanical evaluation through removal torque test to assess the bond between implant and bone, radiographic evaluation through microcomputed tomography analysis to compare the bone-to-implant contact percentage and bone volume of the peri-implant area, scanning electron microscopic and histologic evaluation through hematoxylin and eosin stain. Statistical analyses between pairs of means were conducted using the independent t test. Multiple comparisons between the study groups across time intervals were performed using a 2-way analysis of variance. P-values ≤0.05 were considered statistically significant. Higher removal torque values (P<0.01) were needed to remove the coated implants when biomechanically tested. In addition, the microcomputed tomography evaluation revealed a higher bone-to-implant contact percentage (P<0.05) in favor of coated implants. In addition, the 4-week healing interval of the coated implants showed no significant difference when compared with the 8-week healing interval of the uncoated implants biomechanically and radiographically, suggesting early osseointegration. The findings of this study imply that coating titanium implants with carob-mediated calcium hydroxide nanoparticles improved and fastened osseointegration and bone ingrowth, resulting in early osteogenesis and shortening the treatment time.

Screening of blackgram (Vigna mungo) genotypes for resistance to cercospora leaf spot and yield performance in Nepal

This study screened 12 black gram (Vigna mungo L.) genotypes for resistance to Cercospora leaf spot (CLS) caused by Cercospora canescens. Conducted at the research plot of the Mid-West Academy and Research Institute, Tulsipur, Dang, the experiment utilized a randomized complete block design with three replications from September 2022 to January 2023. Disease severity was evaluated at 40, 47, and 54 days after sowing (DAS), with scoring based on the percentage of infected leaf area at 7-day intervals. Key disease metrics—incidence, severity, mean area under the disease progress curve (AUDPC), and yield—were calculated. Results indicated no significant difference in CLS incidence among genotypes. However, significant differences in disease severity were noted at 40, 47, and 54 DAS, with AUDPC values differing across genotypes. Based on mean AUDPC values, 10 genotypes were categorized as moderately resistant, while BLG 0066-1-1 and BLG 0035-1 were moderately susceptible. BLG 0035-1 had the highest AUDPC (324.1), and BLG 0069-1 had the lowest (175). Yield varied significantly, with the highest yield (799 kg/ha) recorded for BLG 0068-2 and the lowest (495 kg/ha) for BLG 0066-1. These findings support breeding efforts targeting CLS resistance and enhanced yield in black gram.

Subchondral bone marrow adipose tissue lipolysis regulates bone formation in hand osteoarthritis.

Bone marrow adipose tissue (BMAT) is emerging as an important regulator of bone formation and energy metabolism. Lipolysis of BMAT releases glycerol and fatty acid substrates that are catabolized by osteoblasts. Here, we investigated whether BMAT lipolysis is involved in subchondral bone formation in hand osteoarthritis (OA). Subchondral BMAT lipolysis and bone marrow adipocyte (BMAd) morphology were studied in clinical specimens of carpo-metacarpal (CMC1) and distal interphalangeal joint (DIP) OA. BMAd size, osteoblast numbers and expression of lipolysis enzymes (ATGL, phospho-HSL, MGLL) were compared between regions of low and high bone formation. Free fatty acids, glycerol and bone biomarkers were measured in osteochondral explants. Subchondral BMAd size was positively correlated with BMI (r = 0.60, [0.082,0.87]) and reduced in regions of high bone formation (-1149 µm2, [-1977,-726.2]). Osteoblast numbers were negatively correlated with BMAd size (r = -0.48, [-0.73,-0.12]). All lipolysis enzymes were expressed in both in BMAds and activated osteoblasts and the area percentages of ATGL (+2.26% [0.19,3.47]), phospho-HSL (+1.57% [0.31,6.48]) and MGLL (+4.04% [1.09,5.69]) were increased in regions of high bone formation. Secreted glycerol levels, but not free fatty acids, were correlated with bone formation markers pro-collagen type I (rho = 0.90) and alkaline phosphatase (rho = 0.78). Our findings reveal a previously unrecognized role of BMAT lipolysis in regulating bone formation in hand OA, which may be modulated by BMI.

Simplifying complexity: the novel degradation index for urban stream landscapes-case of Tekirdag.

This study aims to examine the degradation process of urban stream landscapes in Tekirdağ by utilizing a newly developed degradation index (DI) that effectively defines degradation. The DI considers the vital factors that affect urban stream landscapes, making it possible to determine the level of degradation and resulting environmental quality. This index aims to provide valuable insight into the degradation of urban stream landscapes with a minimal amount of independent variables and training samples. The key enhancements of the DI include its clear computation of degradation value, ease of replication, and overall objectivity. The results indicate that the most substantial change has been observed on impermeable surfaces between 2000 and 2020, resulting in a 5.18% increase in impervious surfaces. Furthermore, there has been a decrease by 4.67% in agricultural lands, highlighting a pronounced shift towards impervious surfaces. The total percentage of areas categorized as high and very high degradation categories increased by 1.54% over the study period. Additionally, the area classified as high degradation expanded from 135.91 to 375.42 hectares between 2000 and 2020. Notably, there was no land classified as very high degradation in 2000, whereas in 2020, it reached 38.15 hectares. The DI has been proven to provide better representational information on how human activity affects ecosystems compared to both the Human Influence Index and the Human Footprint Index which has been used for this purpose. As a valuable tool for urban planning strategies, the DI can provide decision-makers with a more precise depiction of degradation, aiding in the preservation of sustainable urban stream landscapes, particularly in rapidly urbanizing areas.

Coronary calcium score and emphysema extent on different CT radiation dose protocols in lung cancer screening.

To assess the consistency of automated measurements of coronary artery calcification (CAC) burden and emphysema extent on computed tomography (CT) images acquired with different radiation dose protocols in a lung cancer screening (LCS) population. The patient cohort comprised 361 consecutive screenees who underwent a low-dose CT (LDCT) scan and an ultra-low-dose CT (ULDCT) scan at an incident screening round. Exclusion criteria for CAC measurements were software failure and previous history of CVD, including coronary stenting, whereas for emphysema assessment, software failure only. CT images were retrospectively analyzed by a fully automated AI software for CAC scoring, using three predefined Agatston score categories (0-99, 100-399, and ≥ 400), and emphysema quantification, using the percentage of low attenuation areas (%LAA). Demographic and clinical data were obtained from the written questionnaire completed by each participant at the first visit. Agreement for CAC and %LAA categories was measured by the k-Cohen Index with Fleiss-Cohen weights (Kw) and Intraclass Correlation Coefficient (ICC) with 95% Confidence Interval (CI). An overlap of CAC strata was observed in 275/327 (84%) volunteers, with an almost perfect agreement (Kw = 0.86, 95% CI 0.82-0.90; ICC = 0.86, 95% CI 0.79-0.90), while an overlap of %LAA strata was found in 204/356 (57%) volunteers, with a moderate agreement (Kw = 0.57, 95% CI 0.51-0.63; ICC = 0.57, 95% CI 0.21-0.75). Automated CAC quantification on ULDCT seems feasible, showing similar results to those obtained on LDCT, while the quantification of emphysema tended to be overestimated on ULDCT images. Question Evidence demonstrating that coronary artery calcification and emphysema can be automatedly quantified on ultra-low-dose chest CT is still awaited. Findings Coronary artery calcification and emphysema measurements were similar among different CT radiation dose protocols; their automated quantification is feasible on ultra-low-dose CT. Clinical relevance Ultra-low-dose CT-based LCS might offer an opportunity to improve the secondary prevention of cardiovascular and respiratory diseases through automated quantification of both CAC burden and emphysema extent.