Percentage Of Unsaturated Fatty Acids Research Articles

Fatty acid, triglyceride, and kinetic properties of milk fat fractions made by the combination of dry fractionation and short-path molecular distillation

In this study, we aimed to detect the physicochemical properties of distilled products (residue and distillate) obtained from anhydrous milk fat (AMF) and its dry fractionation products (liquid and solid fractions at 25°C (25 L and 25 S)). The results showed that the saturated fatty acids and low- and medium-molecular-weight triglycerides were easily accumulated in the distillate, and the percentage of unsaturated fatty acid and high-molecular-weight triglycerides in the residue were higher, and these components in 25 S and 25 L were influenced more significantly than those in the AMF. In addition, the distillate had larger melting ranges in comparison with the distilled substrate, while the melting ranges of residue was smaller. The triglycerides were presented as the mixture crystal forms (α, β', and β crystal) in 25 S, AMF, and their distilling products, and it was transformed gradually to a single form as the increasing of distilling temperature. Moreover, the accumulated pattern of triglycerides was double chain length in 25 S, AMF, and their distilling products. These results provide a new approach to obtain the MF fractions with different properties, and the findings of this study enrich the theoretical basis of MF separation in practical production.

Using Sweet Lupine Powder as a Healthy Supplement for the Production of Functional Chicken Burger in Jordan

Chicken meat has grown more popular in recent years because of its great nutritional content,. It has a high protein content as well as a low-fat content and a high percentage of unsaturated fatty acids (polyunsaturated), which have a beneficial effect on consumers' health. Sweet lupine is a leguminous bean of the Fabaceae family. In this study, different proportions of powdered sweet lupine (10, 20, and 35 percent of the mix) were utilized to complement the chicken burgers that were produced in Jordan. The sample enriched with 20% sweet lupine (LB20) was rated as the most popular. The protein level of sweet lupine was 1.3 times that of chicken meat, but the fat amount was similar. The ash concentration of sweet lupine was 2.5 times greater, whereas the fiber content was 55 times higher. The overall carbs content of sweet lupine was 75 times more than that of chicken meat. When total amino acids were calculated, it was approximately the same. The ratio of SFA/USFA assured such finding that was higher with 1.37-fold rather than that of un-supplemented one. The twenty percent addition of sweet lupine as a supplement to chicken burgers gave the best sensory properties and less oxidized fat as seen in when TBARS test.

G0S2 inhibits adipogenesis and unsaturated fatty acid biosynthesis by repressing the expression of SCD in chicken

SummaryFatty acids metabolic products and intramuscular fat (IMF) content determine meat quality in chickens. In this study, the mechanisms of G0/G1 switch gene 2 (G0S2) underlying modulation of chicken IMF content as well as fatty acid synthesis was investigated. Several advanced techniques including Gas Chromatography–Mass Spectrometer (GC–MS), RNA‐sequencing and lentiviral transfection were utilised. RNA‐sequencing showed that 2242 differentially expressed genes were significantly different, and many of these were related to lipid catabolism, such as stearoyl‐CoA desaturase 1 (SCD), acyl‐CoA synthetase bubblegum family member 2 (ACSBG2), and fatty acid desaturase 2 (FADS2), etc. Knocking down of G0S2 also affected the biological activities of signal transduction, catalytic activity, lipid metabolism, cell cycle and death, cell differentiation and immune response. GC–MS revealed that decreased lipid accumulation and lower unsaturated fatty acid percentage were observed in G0S2 knockdown adipocytes. Our results showed that lower expression of G0S2 decreased lipid accumulation mainly via down‐regulated SCD, then regulated mRNA levels of patatin‐like phospholipase domain containing 2 (PNPLA2), which was shown as a major triglyceride lipase in the liver and adipose tissue. Importantly, we further verified the regulation network of G0S2 in the thigh tissues of 12 Huangshan Black chickens, which was consistent with previous IMF results. Thus, our study highlights that G0S2 modulated chicken meat quality traits by the G0S2–SCD–PNPLA2 axis in lipid metabolism in vitro and in vivo. These findings are expected to provide a strategy from a genetic perspective for improving the meat quality.

An Acyl Carrier Protein Gene Affects Fatty Acid Synthesis and Growth of Hermetia illucens

Acyl carrier protein (ACP) is an acyl carrier in fatty acid synthesis and is an important cofactor of fatty acid synthetase. Little is known about ACP in insects and how this protein may modulate the composition and storage of fatty acids. We used an RNAi-assisted strategy to study the potential function of ACP in Hermetia illucens (Diptera: Stratiomyidae). We identified a HiACP gene with a cDNA length of 501 bp and a classical conserved region of DSLD. This gene was highly expressed in the egg and late larval instars and was most abundant in the midgut and fat bodies of larvae. Injection of dsACP significantly inhibited the expression level of HiACP and further regulated the fatty acid synthesis in treated H. illucens larvae. The composition of saturated fatty acids was reduced, and the percentage of unsaturated fatty acids (UFAs) was increased. After interfering with HiACP, the cumulative mortality of H. illucens increased to 68.00% (p < 0.05). H. illucens growth was greatly influenced. The development duration increased to 5.5 days, the average final body weights of larvae and pupae were decreased by 44.85 mg and 14.59 mg, respectively, and the average body lengths of larvae and pupae were significantly shortened by 3.09 mm and 3.82 mm, respectively. The adult eclosion rate and the oviposition of adult females were also severely influenced. These results demonstrated that HiACP regulates fatty acid content and influences multiple biological processes of H. illucens.

Protective Properties of Copper-Loaded Chitosan Nanoparticles against Soybean Pathogens Pseudomonas savastanoi pv. glycinea and Curtobacterium flaccumfaciens pv. flaccumfaciens.

Soybeans are a valuable food product, containing 40% protein and a large percentage of unsaturated fatty acids ranging from 17 to 23%. Pseudomonas savastanoi pv. glycinea (Psg) and Curtobacterium flaccumfaciens pv. flaccumfaciens (Cff) are harmful bacterial pathogens of soybean. The bacterial resistance of soybean pathogens to existing pesticides and environmental concerns requires new approaches to control bacterial diseases. Chitosan is a biodegradable, biocompatible and low-toxicity biopolymer with antimicrobial activity that is promising for use in agriculture. In this work, a chitosan hydrolysate and its nanoparticles with copper were obtained and characterized. The antimicrobial activity of the samples against Psg and Cff was studied using the agar diffusion method, and the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined. The samples of chitosan and copper-loaded chitosan nanoparticles (Cu2+ChiNPs) significantly inhibited bacterial growth and were not phytotoxic at the concentrations of the MIC and MBC values. The protective properties of chitosan hydrolysate and copper-loaded chitosan nanoparticles against soybean bacterial diseases were tested on plants in an artificial infection. It was demonstrated that the Cu2+ChiNPs were the most effective against Psg and Cff. Treatment of pre-infected leaves and seeds demonstrated that the biological efficiencies of (Cu2+ChiNPs) were 71% and 51% for Psg and Cff, respectively. Copper-loaded chitosan nanoparticles are promising as an alternative treatment for bacterial blight and bacterial tan spot and wilt in soybean.

The Effects of Different LED Lights on the Main Nutritional Compositions of Isochrysis zhanjiangensis

Light irradiation plays an important role in the growth of microalgae and their nutrient composition. To elucidate the effect of different LED lights (red, orange, green, blue, purple, simulated sunlight, and white) on the main nutritional compositions of Isochrysis zhanjiangensis, the growth, fatty acid composition, soluble carbohydrate, and soluble protein contents were studied. The results show that I. zhanjiangensis grew the fastest under blue light (p = 0.044). In terms of fatty acid composition, the percentage of myristic acid (C14:0) decreased significantly (p = 0.021) under blue light and simulated sunlight, but the percentages of palmitic acid (C16:0) (p = 0.032) and stearic acid (C18:0) (p = 0.037) significantly increased. The percentage of docosahexaenoic acid (C22:6n-3) increased under orange light (p = 0.021), and the percentage of total unsaturated fatty acids increased under blue light (p = 0.008). The protein content significantly increased under simulated sunlight (p = 0.025), while the carbohydrate content did not vary much (p &gt; 0.05) under different lights. These findings provide useful guidance for optimizing the cultivation conditions of I. zhanjiangensis to improve its nutritional value and yield.

Rabbit Meat—Production, Consumption and Consumers’ Attitudes and Behavior

Rabbit meat could play an important role in health, the rural economy, and sustainable development. Rabbit meat has excellent nutritional features, such as high protein content, low-fat content, and a high percentage of unsaturated fatty acids, low cholesterol and sodium levels. In addition, rabbit meat production contributes to maintaining economic activities in rural marginal areas. However, the consumption of rabbit meat is still limited due to several factors such as the higher cost of commercial food that is slowing down rabbits’ breeding. Socio-demographic characteristics, attitudes, and nationality of consumers influence the demand and consumption of rabbit meat. The social and economic changes of the past years are leading to an increased interest in rabbit meat products with convenience characteristics. Consumers are also increasingly paying attention to animal husbandry methods for health concerns and ethical reasons. This paper presents an overview of rabbit meat focusing on production, nutritional composition, consumers’ preferences, and marketing. The review proposes strategies that, coupled with information campaigns could improve consumers’ knowledge of the positive characteristics of rabbit meat, which would contribute to the market development.

Gas chromatography/mass spectrometry with chemometric methods for the determination of fatty acid profiles in herbal oils.

The conventional method for analyzing fatty acid is gas chromatography (GC) with polar capillary columns. However, owing to the high cost and the sensitivity of these columns to the presence of water and oxygen, these columns are not the best choice for separation. Also, analyzing long-chain acids (>C28) with polar columns is impossible. On the other hand, complete separation with nonpolar columns is not possible for some saturated, unsaturated and long-chain fatty acids. Therefore, in this study, with the help of chemometric methods, a method was developed using GC/mass spectrometry (MS) with a nonpolar column to resolve the peaks to completely separate and accurately identify and quantify fatty acids. Using this method, the fatty acid profiles of the seed oils of Sesamum indicum L, Nigella sativa, Pimpinella anisum, Linum asitatissimum L, Silybum marianum and Amygdalus communis L. var. Amara and var. Dulcis were identified. Through applying the multivariate curve resolution method after GC/MS, the C-18 fatty acids such as α-linolenic, linoleic, oleic and stearic acids were separated and quantified. Also, the total percentages of identified fatty acids increased by 1-6% after resolving overlapping peaks. Finally, the obtained percentages of saturated and unsaturated fatty acids were confirmed by reference reports.

Undesirable consequences of the metallic nanoparticles action on the properties and functioning of Escherichia coli, Bacillus cereus and Staphylococcus epidermidis membranes

Controversial and inconsistent findings on the toxicity of metallic nanoparticles (NPs) against many bacteria are common in recorded studies; therefore, further advanced experimental work is needed to elucidate the mechanisms underlying nanotoxicity. This study deciphered the direct effects of Ag-NPs, Cu-NPs, ZnO-NPs and TiO2-NPs on membrane permeability, cytoplasmic leakage, ATP level, ATPase activity and fatty acid profiling of Escherichia coli, Bacillus cereus and Staphylococcus epidermidis as model microorganisms. A multifaceted analysis of all collected results indicated the different influences of individual NPs on the measured parameters depending on their type and concentration. Predominantly, membrane permeability was correlated with increased cytoplasmic leakage, reduced total ATP levels and ATPase activity. The established fatty acid profiles were unique and concerned various changes in the percentages of hydroxyl, cyclopropane, branched and unsaturated fatty acids. Decisively, E. coli was more susceptible to changes in measured parameters than B. cereus and S. epidermidis. Also, it was established that ZnO-NPs and Cu-NPs had a major differentiating impact on studied parameters. Additionally, bacterial cell imaging using scanning electron microscopy elucidated different NPs distributions on the cell surface. The presented results are believed to provide novel, valuable and accumulated knowledge in the understanding of NPs action on bacterial membranes.

Linoleic acid enrichment of cheese by okara flour and Geotrichum candidum overexpressing Δ12 fatty acid desaturase.

Mold-ripened cheeses have low levels of unsaturated fatty acids (UFAs). Geotrichum candidum is an adjunct culture for the development of Geotrichum-ripened cheese but has a low ability to produce high levels of UFAs. Δ12 fatty acid desaturase (FADS12) is a pivotal enzyme that converts oleic acid (OA) to linoleic acid (LA) and plays a vital role in UFA biosynthesis. By investigating FADS12 catalytic activity from various species with OA substrates, we found that FADS12 from Mucor circinelloides (McFADS12) had the highest catalytic activity for OA. In the current study, a plasmid harboring McFADS12 was constructed and overexpressed in G. candidum. Our results showed that LA production increased to 31.1 ± 1.4% in engineered G. candidum - three times higher than that in wild-type G. candidum. To enhance LA production, an exogenous substrate (OA) was supplemented, and the yield of LA was increased to 154 ± 6 mg L-1 in engineered G. candidum. Engineered G. candidum was used as an adjunct culture for Geotrichum-ripened cheese production. The LA level reached 74.3 ± 5.4 g kg-1 cheese, whereas the level of saturated fatty acids (SFAs) decreased by 9.9 ± 0.5%. In addition, the soybean byproduct (okara) was introduced into the engineered G. candidum growth and the level of LA increased to 126 ± 4 g kg-1 cheese and the percentage of UFAs:SFAs increased from 0.8:1 to 1.3:1. This study offers a suitable technology for converting SFAs to UFAs in Geotrichum-ripened cheeses and provides a novel trend for converting soybean waste into a value-added product. © 2022 Society of Chemical Industry.

Dietary metabolizable energy and crude protein levels affect pectoral muscle composition and gut microbiota in native growing chickens

The experiment aimed to study effects of dietary metabolizable energy (ME) and crude protein (CP) levels alone and in interaction on performance, pectoral muscle composition and gut microbiota in native growing chickens. A total of 648 10-wks-old Beijing-You Chicken (BYC) female chickens were randomly allocated to 9 groups with 6 replicates per group and 12 chickens per replicate, and the chickens were fed with a 3×3 factorial diets (3 levels of dietary ME: 11.31 MJ/kg, 11.51 MJ/kg, 11.71 MJ/kg; and 3 levels of dietary CP: 14%, 15%, 16%). The results showed that dietary ME and CP levels didn't affect average feed intake (AFI), body weight gain, feed gain ratio (P > 0.05), but ME level significantly affected the AFI (P < 0.05); mortality rate of 11.31 MJ/kg group was the highest (P < 0.05). Dietary ME, CP levels, and the interaction significantly affected pectoral CP and crude fat (CF) content of the growing chickens (P < 0.01). Dietary CP level had opposite effects on pectoral CP and CF content (P < 0.01). The 16% CP increased the pectoral CF content, which may have a negative impact on meat flavor. Dietary ME level affected 11 types of pectoral free amino acids (FAA) contents, including aspartic acid, L-threonine (P < 0.05), also amino acid classification, for example, total amino acid (TAA) and essential amino acid (EAA) content (P < 0.05). The 11.51 MJ/kg group had the highest TAA, EAA, delicious amino acid (DAA) content and EAA percentage (P < 0.05), while 11.31 MJ/kg group had the lowest bitter amino acid (BAA) content and BAA percentage and the highest fresh and sweet amino acid (FSAA) percentage (P < 0.05). Dietary CP level significantly affected glutamine and tyrosine content (P < 0.05). The interaction of dietary ME and CP level affected C20:3n6 content, saturated fatty acid (SFA), and unsaturated fatty acid (UFA) percentage (P < 0.05). The CP level significantly affected SFA percentage (P < 0.05). The 16% CP level increased the diversity of gut microbiota, but at the same time increased the relative abundance of Proteobacteria (P < 0.05), which is a sign of microbiota disorder. The increase of dietary ME level resulted in a gradual decrease in the diversity and relative abundance of gut microbiota. In conclusion, the present study suggested that the medium dietary ME (11.51 MJ/kg) and low CP (14-15%) levels can be helpful for enhancing pectoral muscle composition, increase meat quality such as flavor and nutritional value, and benefit for gut microbiota in native growing chickens.

Effects of Feeding Regimes and Postmortem Aging on Meat Quality, Fatty Acid Composition, and Volatile Flavor of Longissimus Thoracis Muscle in Sunit Sheep.

The effects of different feeding regimes on antioxidant activity, meat quality, fatty acid composition, lipid oxidation, and volatile matter production in the longissimus thoracis (LT) of Sunit sheep at 0, 24, 48, 72, and 96 h postmortem were investigated. The results showed that the activity of antioxidant enzymes, tenderness, water retention, and percentage of unsaturated fatty acids were significantly higher in the pasture-fed sheep (PF) than in the concentrate-fed sheep (CF) (p &lt; 0.05). During postmortem aging, antioxidant activity, water retention, and the proportion of unsaturated fatty acids decreased in the PF and CF (p &lt; 0.05), while malondialdehyde (MDA) content, the proportion of saturated fatty acids, and the content of flavor substances resulting from fat oxidation increased. After 24 h of LT muscle aging, the pH and shear force of the meat started to increase and the color stabilized. The differences between shear force values and lipid volatile flavor substance content of sheep meat under different feeding regimes disappeared with increasing aging time. PF had better oxidative stability and fatty acid composition. Postmortem aging changed the oxidative stability of sheep meat, thus affecting meat quality and fatty acid composition and consequently meat flavor composition, while aging also eliminated to some extent the differences caused by feeding regimes.

Walnut fruit: Impact of ethylene assisted hulling on in vitro antioxidant activity, polyphenols, PUFAs, amino acids and sensory attributes

The effects of three different hulling methods wiz natural heaping/traditional (T1), steeping (T2), and spraying (T3), on moisture, colour, fat, free amino acids, fatty acids, antioxidant activities, flavonoids, tannins, total phenolic content, and organoleptic qualities of walnut kernels were examined in this study. The kernels extracted from walnuts subjected to T3 method recorded significantly (p ≤ 0.05) highest DPPH inhibition (68.61 %), ABTS (54.56 %inhibition) and FRAP (0.106 μM trolox/100 g); flavonoids (1993.08 mg QE/100 g), tannin content (0.312 %) and phenols (0.736 mg GAE/g) compared to T1 and T2. Walnut kernels of T3 treatment group were rated better in terms of taste (3.8), odour (3.6) and overall acceptability (3.78) Furthermore, walnut kernels obtained from T3 treatment group exhibited the highest percentage of unsaturated fatty acids (UFAs) and sweet free amino acids. The outcome of the present study offers a fresh viewpoint regarding the hulling processes of green walnuts to meet the quality requirements of walnut kernels.

Growth performance and nutrient composition of black soldier fly larvae reared on solid-state fermentation substrates with various white rot fungi

Abstract. Fitriana EL, Jayanegara A, Astuti DA, Laconi EB. 2022. Growth performance and nutrient composition of black soldier fly larvae reared on solid-state fermentation substrates with various white rot fungi. Biodiversitas 23: 4894-4905. This study examines agricultural byproducts fermented with various rot fungi as a substrate of black soldier fly larvae. The experiment used a three-way randomized design with four replications. The first factor was substrate (cacao pod husk and oil palm frond), the second factor was fungi (Phanerochaete chrysosporium, Trametes versicolor, and Pleurotus sajor-caju), and the third factor was environment condition (light and without light). Approximately 1200 of 7-day-old larvae were added to 600 g substrate treatment. The results showed that the solid-state fermentation substrates had the highest waste reduction parameters and level of consumption. Larvae in the experiment had a crude protein level of 38.84-58.88% of dry matter. Glutamic acid is the most abundant non-essential amino acid in larvae, while leucine is the most abundant essential amino acid. Solid-state fermentation substrate could improve the fatty acid profile quality of larvae in relation to high linoleic acid, polyunsaturated fatty acid, and unsaturated fatty acid percentages, especially CPH-pc substrate. The use of solid-state fermentation in larvae rearing decreased lignin in the cacao pod husk substrate. In conclusion, cacao pod husk substrate solid-state fermented with white rot fungi is not supported the optimum growth performance of BSFL but increases fatty acid profile quality.

Synthesis of Jatropha-Oil-Based Polyester Polyol as Sustainable Biobased Material for Waterborne Polyurethane Dispersion.

The utilization of vegetable oil in the production of polymeric material has gained interest due to its proven ability to replace nonrenewable petroleum sources, as it is readily modified via chemical reaction to produce polyol and subsequently for polyurethane production. Jatropha oil (JO), a second-generation feedstock, is one of the suitable candidates for polyester polyol synthesis because it contains a high percentage of unsaturated fatty acids. In this study, jatropha-based polyester polyols (JOLs) with different hydroxyl values were successfully synthesized via a two-step method: epoxidation followed by oxirane ring-opening reaction. Ring-opening reagents; methanol, ethanol, and isopropanol were used to produce polyol with hydroxyl number of 166, 180, and 189 mg/KOH, respectively. All the synthesized JOLs exhibited a Newtonian to shear thinning behavior in the measured shear rate ranges from 10 to 1000 s−1 at 25 °C. The viscosity of a JOL ring-opened with methanol, isopropanol, and ethanol was 202, 213, and 666 mPa·s, respectively, at 20 °C and 100 s−1, which is within the range of commercially available polyols. Successively, the JOLs were reacted with isophorone diisocyanate (IPDI) to produce polyurethane prepolymer by utilizing 2,2-dimethylol propionic acid (DMPA) as an emulsifier. The prepolymer was then dispersed in water to produce a waterborne polyurethane dispersion. Colloidal stability of the jatropha-based polyurethane dispersions (JPUDs) were investigated by particle size analysis. A JPUD with a small particle size in the range of 6.39 to 43.83 nm was obtained, and the trend was associated with the soft segment of the polyol in the formulation. The zeta potentials of the JPUs ranged from −47.01 to −88.9 mV, indicating that all synthesized JPUs had high dispersity and stability. The efficient synthesis procedure, low cost, and excellent properties of the resulting product are thought to offer an opportunity to use jatropha oil as a sustainable resource for polyester polyol preparation.

New concept of biodiesel production using food waste digestate powder: Co-culturing algae-activated sludge symbiotic system in low N and P paper mill wastewater

This paper aims to demonstrate an innovative process for the conversion of food waste digestate (FWD) powder into biofuel. The effects of different doses of FWD are investigated on microalgae-activated sludge (MAS) in treating pulp and paper mill wastewater (PPW) which generally contains insufficient nitrogen and phosphorus. FWD was added to adjust the initial N:P molar ratio in MAS at various levels (8:1 to 15:1). The highest Auxenochlorella protothecoides biomass achieved was 1.67 gL−1 at a 13.45:1 N/P molar ratio of PPW. After 10 days of cultivation, Auxenochlorella protothecoides-activated sludge system removed 91.7 %, 74.6 %, and 91.5 % of total nitrogen, phosphorus, and sCOD respectively at D0.836 gL−1 DD. The highest lipid productivity was reported as 41.27 ± 2.43 mg L−1 day−1. Fatty acid methyl ester (FAME) analysis showed the presence of an appreciable percentage of balanced saturated and unsaturated fatty acids i.e. palmitic, oleic, and linoleic acid, rendering its potential as a feedstock for biodiesel production. Activated sludge induced flocculation of Auxenochlorella protothecoides was measured. The whole process establishes an effective means of circular economy, where the secondary source of recyclable nutrients i.e. FWD will be used as a source of N and P in PPW to obtain algal biodiesel from a negative value industrial wastewater.

Chitosan/Calcium-Alginate Encapsulated Flaxseed Oil on Dairy Cattle Diet: In Vitro Fermentation and Fatty Acid Biohydrogenation.

Simple SummaryMost unsaturated fatty acids in the ruminant’s diet are hydrogenated in their rumen so that the composition of the fatty acids entering the rumen and their output is significantly different. Therefore, minimizing the ruminal biohydrogenation process of unsaturated fatty acids is one of the most important issues for feed supplements manufacturers and animal nutritionists to increase the availability of these fatty acids in the intestine. In recent years, encapsulation has been used to preserve the active ingredient in livestock; it is a method used to control the release of feed additives during digestion. There is a clear need to find a more effective method by which unsaturated fatty acids present in fat supplements can be protected to bypass rumen environment and its biohydrogenation, without negative effect on digestive efficiency, and be available in lower digestive tracts. For these reasons, this study aims to evaluate the use of natural materials to encapsulate fats and their effect on in vitro fermentation and fatty acid biohydrogenation. The results indicated that the percentage of ruminal saturated fatty acids decreased by encapsulation of flaxseed oil with chitosan (14% and 7%). The percentage of oleic unsaturated fatty acid by encapsulating flaxseed oil with chitosan (14%) had a significant increase compared to the control treatment (p < 0.05). Encapsulation of flaxseed oil with chitosan (14%) reduced the unsaturated fatty acids of ruminal biohydrogenation.The aim of this study was to investigate the effect of using chitosan nanoparticles and calcium alginate in the encapsulation of flaxseed oil on the biohydrogenation of unsaturated fatty acids and in vitro fermentation. The experiments were performed in a completely randomized design with 7 treatments. The experimental treatments included: diets without oil additive (control), diet containing 7% flaxseed oil, diet containing 14% flaxseed oil, diet containing 7% oil encapsulated with 500 ppm chitosan nanocapsules, diet containing 14% flaxseed oil encapsulated with 1000 ppm chitosan nanocapsules, diet containing 7% of flaxseed oil encapsulated with 500 ppm of calcium alginate nanocapsules, diet containing 14% flaxseed oil encapsulated with 1000 ppm calcium alginate nanocapsules. The results showed that encapsulation of flaxseed oil with calcium alginate (14%) had a significant effect on gas production (p < 0.05). The treatment containing calcium alginate (14%) increased the digestibility of dry matter compared to the control treatment, but the treatments containing chitosan caused a significant reduction (p < 0.05). The results indicated that the percentage of ruminal saturated fatty acids decreased by encapsulation of flaxseed oil with chitosan (14% and 7%). The percentage of oleic unsaturated fatty acid by encapsulating flaxseed oil with chitosan (14%) had a significant increase compared to the control treatment (p < 0.05). As a result, encapsulating flaxseed oil with chitosan (14%) reduced the unsaturated fatty acids generated during ruminal biohydrogenation.

Identification of aerobic-denitrifying Psychrobacter cryohalolentis strain F5-6 and its nitrate removal at low temperature

Biological denitrification is suppressed when temperature is lower than 10 °C. In this study, Psychrobacter cryohalolentis F5-6 was isolated from Harbin region and showed high aerobic-denitrifying rate at low temperature. It contained high percentage of unsaturated fatty acids (87.32%) and short chain saturated acid (3.4% C10:0 and 4.0% C12:0 3-OH). Acetate was the best carbon source for the strain F5-6 performing denitrification. Aerobic denitrification (napA gene) and nitrate assimilation (nasA gene) were the nitrate metabolism processes for the strain F5-6. Its biological nitrate removal (BNR) rate at 10 °C showed fit with Monod equation (R2 = 0.9808). The maximum BNR rate was 23.71 mg NO3−-N g−1DW h−1 and KS was 116.96 mg L−1. The optimal denitrifying condition was pH 7.5, 15 °C with shaking speed of 180 rpm and C/N of 2. Results of this study supplied a bacterial resource for improving nitrate removal from low C/N water at low temperature.

Potential Inhibitors of CYP51 Enzyme in Dermatophytes by Red Sea Soft Coral Nephthea sp.: In Silico and Molecular Networking Studies.

In this study, the n-hexane fraction of soft coral Nephthea sp. gathered from the Red Sea was evaluated for its antidermatophyte activity. The antidermatophyte activity was performed versus different fungi, including Microsporum canis, Trichophyton gypseum, and Microsporum mentagrophytes, using a broth microdilution method. The n-hexane fraction showed minimum inhibitory concentrations (MICs) against the tested dermatophytes of 104.2 ± 20.8, 125 ± 0.0, and 83.33 ± 20.83 μg/mL respectively. The chemical constitution of the lipoidal matter (n-hexane fraction) was characterized by gas chromatography coupled with a mass spectrometer (GC-MS). The unsaponifiable fraction (USAP) of Nephthea sp. showed relative percentages of hydrocarbons and vitamins of 69.61% and 3.26%, respectively. Moreover, the percentages of saturated and unsaturated fatty acids were 53.67% and 42.05%, respectively. In addition, a molecular networking study (MN) of the GC-MS analysis performed using the Global Natural Products Social Molecular Networking (GNPS) platform was described. The molecular docking study illustrated that the highest binding energy score for spathulenol toward the CYP51 enzyme was −8.3674 kcal/mol, which predicted the mode of action of the antifungal activity, and then the results were confirmed by the inhibitory effect of Nephthea sp. against CYP51 with an IC50 value of 12.23 μg/mL. Our results highlighted the antifungal potential of Nephthea sp. metabolites.

Comparison of Muscle Quality of the Yellow Catfish Cultured in In-Pond Raceway Systems and Traditional Ponds

In order to understand the difference in muscle nutritional quality between yellow catfish (Pelteobagrus fulvidraco) cultured in in-pond raceway systems (IPR) and traditional ponds (TRP), two modes were used to culture the yellow catfish with the same initial body weight [average body weight (15.69 1 ± 2.28) g] for 60 days. The growth index, muscle texture characteristics, muscle nutritional components, amino acids and fatty acids of the fish were measured after the culture experiment. The results showed that the weight gain rate, specific growth rate and survival rate of IPR were significantly higher than TRP (p &lt; 0.05). The hardness, elasticity, chewiness and resilience of the yellow catfish cultured in IPR were significantly higher than those cultured in TRP (p &lt; 0.05). The crude protein content in the muscle of the yellow catfish cultured in IPR was significantly higher than that cultured in TRP (p &lt; 0.01), while the content of crude fat and water was significantly lower (p &lt; 0.01). The total amount of amino acids, essential amino acids and flavor amino acids of IPR were significantly higher than TRP (p &lt; 0.01). The percentages of saturated fatty acids in muscle of the yellow catfish cultured in IPR and TRP were 3.59% and 3.83%, respectively, and the percentages of unsaturated fatty acids were 96.41% and 96.17%, respectively. It was concluded that the nutritional quality of yellow catfish cultured in IPR was better than that of fish cultured in TRP.