Percent Ethanol Research Articles

Determination of the percentage of ethanol produced by Saccharomyces cerevisiae from semi-purified glycerin

The percentage of ethanol produced by Saccharomyces cerevisiae yeast in semi-purified glycerin at three concentrations (2.8%, 5%, 10%) was determined. Two phases were established: an aerobic phase evaluating temperatures of 20°C and 37°C with an inoculation rate of 4% and 1vvm (volume/volume of medium per minute) and an anaerobic phase at a temperature of 10°C, which were monitored for a period of 36 hours. The main kinetic parameters such as specific growth rate, doubling time and the percentage of ethanol produced by the Winnick technique were determined. The results showed that the best temperature to produce biomass of S. cerevisiae is given around 20°C with concentrations of 1.30x108cells/ml with a maximum peak biomass production at 21 hours, which differ significantly from the other treatments evaluated (Sig. = 0.000). The highest production of ethanol was obtained in the treatment of 20% glycerin and 10% of inoculum of the microorganism (p<0.05) at a temperature of 10°C, corresponding to 176.87mg of ethanol/ml of glycerin. Therefore, the viability of this agroindustry by-product is deduced as an alternative source for the biomass and ethanol production from Saccharomyces cerevisiae.

Prenatal ethanol exposure potentiates isolation-induced ethanol consumption in young adult rats.

Prenatal and/or early postnatal ethanol exposure (PEE) is associated with significant behavioral and physiological deficits in offspring, including alterations in stress response systems and a greater likelihood of alcohol use disorders. Stress-induced ethanol drinking after PEE, however, has been largely unexplored. The present study analyzed ethanol intake in male Sprague-Dawley rats after protracted prenatal and early postnatal ethanol exposure and tested whether social isolation during the sensitive period of adolescence modulates the effects of PEE on ethanol drinking. The dams were given 10% ethanol (or its vehicle) as the sole drinking fluid from gestational day 0 (GD0) to postnatal day 7 (PD7). On PD21, male offspring were housed individually (isolated housing group) or in pairs in standard cages (standard housing group). From PD56 to PD84, these male rats were tested for ethanol intake in 24-h, intermittent two-bottle choice sessions that were conducted across 4 weeks. Maternal ethanol consumption during gestation and during the first week of life of the offspring averaged 6.10-8.20g/kg/22h. Isolation housing during adolescence increased free-choice ethanol drinking in young adulthood. The main novel finding was that this facilitative effect of isolation on absolute and percent ethanol intake was significantly greater in PEE rats than in control counterparts not exposed to the prenatal and early postnatal ethanol exposure (effect sizes [η2p]: 0.24-0.32). The present results suggest that PEE renders the individual sensitive to the facilitative effect of stress exposure on ethanol intake.

Mass transfer intensification in bioactive compounds recovery by alternative extraction methods: effects of solvent

&lt;p&gt;Recently, the exploration of new alternatives to common organic solvents in combination with non-conventional energy sources is a subject of intense research and development as tools for green extraction of plant-derived natural products. This paper deals with the use of low frequency ultrasound (100 W, 32 kHz) and pulsed electrical fields (0.86 kV cm-1, 13 Hz) to raise the efficiency of the extraction of selected plant species rich in valuable phenolic compounds.Four types of natural deep eutectic solvents were applied for the ultrasound-assisted extraction of silymarin from milk thistle among which a choline-chloride-based mixture with glycerol was proved to be the most promising one. Additionally, the usage of high ultrasound power significantly increased the concentration of sylimarin as compared to traditional ethanol extraction, generating rapidly for 10 min considerable temperature rise as a side effect under non-cooling conditions. The application of PEF as a pre-treatment for 0.8 s to the extraction allowed reducing the ethanol percentage in the ethanol/water mixtures giving comparable phenolic yields, without any, or very little, increase in temperature. As such, optimized power intensity in conjunction with appropriate solvent system provides a potential to improve the release of thermally unstable bioactive compounds under mild processing conditions.&lt;/p&gt;

Influence of dodecanol addition on Cold Filter Plugging Point for the mixtures of diesel oil with ethanol

The article presents the results of tests which were supposed to determine the influence of dodecanol addition to diesel oil on Cold Filter Plugging Point (CFPP). Tested were the mixtures of diesel oil and ethanol, with the volumetric percentage of ethanol of 5, 10 and 15%. The maximum dodecanol addition represented 20%. Determination of the temperature at which the filter got plugged was carried out with the use of the FPP 5Ds device, and the CFPP determination was carried out in accordance with the PN-EN 116: 2015-09 standard. The test results reflect, among other things, that dodecanol addition to the mixture of diesel oil with dehydrated ethanol results in lowering the Cold Filter Plugging Point for the lowest of the tested dehydrated ethanol percentages, whereas increase in the CFPP for the other percentages. As regards the mixtures with hydrated ethanol in which 20% dodecanol addition was applied, it may be noticed that along with the growth of the hydrated ethanol content, the CFPP was decreasing.

&lt;b&gt;The effects of ultrasound-assisted extraction on polyphenolics compounds obtained from &lt;i&gt;Physalis angulata&lt;/i&gt; using response surface approach

Total polyphenols content (TPC), including flavonoids (rutin, mangiferin and kaempferol) and phenolic acids (gallic, caffeic and ellagic acid) from Physalis angulata were recovered by Ultrasound-Assisted Extraction and quantified by UV–vis and HPLC analysis. Process parameters were assessed through a Central Composite Rotatable Design (CCRD) and a model with regression coefficient equal to 0.9640, was used to establish the optimum conditions together with its respective response surfaces. The increase of ethanol percentage and solid-liquid ratio promoted a decrease on TPC but, on the other hand, the increase in the temperature led to an increase in the extraction of these compounds. Experimental results indicated a maximum amount of total polyphenols of 1.039 mg Gallic Acid Eqivalent (GAE) g-1 of extract, 104.88, 4.04, 8.37, 58.28, 13.26 and 1.87mg.L-1 for gallic acid, caffeic acid, ellagic acid, rutin, mangiferin and kaempferol, respectively.

Evaluation on physicochemical properties of iso-butanol additives in ethanol-gasoline blend on performance and emission characteristics of a spark-ignition engine

In this study, experiments were conducted on a four-cylinder spark-ignition engine to investigate the effects of iso-butanol additives in ethanol-gasoline blend on fuel properties, performance and emission characteristics of a SI engine. The engine tests were carried out at 50% wide open throttle and variations of engine speed from 3000 to 5000 RPM with an interval of 1000 RPM. The engine was fueled with base gasoline fuel, ethanol-gasoline blended fuel at 10% volume percentage of ethanol (E10) and three different fuel blends of iso-butanol additives; 5%, 10% and 15%, in E10 blended fuel denoted as E10B5, E10B10 and E10B15, respectively. Physicochemical properties tests were conducted in this study to evaluate the fuel heating value, kinematic viscosity and density, which have been selected due to their influences on engine performance and exhaust emissions. Results of heating value showed a decreasing pattern for the blended fuel samples compared with that of base gasoline fuel as E10, E10B5, E10B10 and E10B15 produced 2.33%, 3.14%, 5.06%, and 5.31%, respectively. However, as alcohol concentration increases in the blended fuel samples, the density and kinematic viscosity were both increased with maximum values of 0.767 g/cm3 and 1.15 mm2/s, respectively obtained for E10B15. In terms of engine performance, the blended fuel samples exhibited higher brake power than that of base gasoline fuel with mean increase of 7.71%, 10.21%, 10.89% and 11.54% for E10, E10B5, E10B10 and E10B15, respectively. Significant reduction in brake specific fuel consumption obtained with E10, E10B5, E10B10 and E10B15 by mean of 3.57%, 5.15%, 7.14% and 10.89% respectively, compared to base gasoline fuel. Noticeable improvement of brake thermal efficiency observed with the blended fuel at a maximum increment of 18.91% achieved by E10B15. High combustion temperature produced by the blended fuel samples have been contributed to the higher exhaust gas temperature. Accordingly, the emissions of carbon monoxide and hydrocarbon were all reduced; except for carbon dioxide and nitrogen oxide, with the addition of iso-butanol additive compared to those of ethanol-gasoline blend and base gasoline fuel.

A 30-s exposure to ethanol 20% is cytotoxic to human keratinocytes: possible mechanistic link between alcohol-containing mouthwashes and oral cancer.

To provide mechanistic evidence for the epidemiological link between long-term use of alcohol-containing mouthwashes and oral cancer. Human epithelial keratinocytes were exposed for 30s to concentrations of ethanol commonly present in mouthwashes. After a recovery period, cell viability was assessed with the MTT assay. A marked cytotoxic effect was observed for ethanol concentrations of 20% and above. The cytotoxicity of ethanol may explain the epidemiological association between mouthwash use and oral cancer. Evidence suggests that the risk of developing cancer in a tissue is strongly determined by the number of stem cell divisions accumulated by the tissue during a person's lifetime; cell division is a major source of mutations and other cancer-promoting errors. Since cell death activates the division of stem cells, the possible cytotoxicity of ethanol on the cells lining the oral mucosa will promote the division of the stem cells located in deeper layers to produce new cells to regenerate the damaged epithelium. If we regularly use mouthwashes containing cytotoxic concentrations of ethanol, the stem cells of the oral cavity may need to divide more often than usual and our risk of developing oral cancer may increase. Many mouthwashes contain percentages of ethanol above 20%. Because ethanol is not crucial to prevent and reduce gingivitis and plaque, members of the dental team should consider the potential risk of oral cancer associated with frequent use of alcohol-containing mouthwashes when advising their patients.

Enhanced ethylene production by dehydration of ethanol over Al/SBA-15 mesoporous catalysts

Dehydration of ethanol into ethylene was studied over Al/SBA-15 at the temperature range 250–450 °C. A novel technique was developed for synthesis of three kinds of Al/SBA-15 as mesoporous catalysts by modifying the reported direct and post synthesis procedures. The neat SBA-15 as well as Al/SBA-15 were characterized adopting several characterization techniques viz., N2 adsorption–desorption, XRD, TGA–DSC, FT-IR, TEM, EDX and NH3-TPD. Incorporation of Al into modified SBA-15, namely, Al/SBA-15(Inst), prepared by in situ Al grafting direct method, could result in a marked acidity referred to the presence of stronger Bronsted acid sites and an increased surface area (880 m2/g) reflecting on the catalyst functionality. As a consequence, this Al–SBA/15(Inst) catalyst sample exhibited the highest activity toward ethylene production with a maximum yield of (100%) at 400 °C. The percent ethanol conversion at the same reaction temperature decreased on the studied catalysts in the order: Al–S(Inst) prepared by in situ direct modification > Al–S(Imp) produced by impregnation post synthesis > Al–S(Us) obtained through ultrasonic irradiation > neat SBA-15. All the modified catalysts achieved 100% selectivity which was stabilized over the whole temperature range used. They may be considered thus as highly promising candidates for selective ethanol dehydration into ethylene.

Effects of biodiesel-ethanol-diesel blends on the performance indicators of a diesel engine: A study by response surface modeling

The objective of the present study is to evaluate the effect of several variables, including different levels of biodiesel and ethanol in diesel blended fuel (biodiesel-ethanol-diesel blends), engine load and rotational speed, on the performance indicators of a diesel engine. Design of experiments was conducted as central composite rotatable designs using response surface method. The results showed that increasing biodiesel and/or ethanol percentages in the fuel blends caused to reduce the engine brake power. A low level of biodiesel and/or ethanol in the fuel blends could enhance the engine brake thermal efficiency in comparison with the pure diesel fuel or high level of biodiesel and/or ethanol in the diesel fuel blends. The highest value of the engine brake power occurred on the condition for pure diesel, engine load of 100% (full load) and rotational speed of 2800 rpm, whereas the highest value of thermal efficiency was observed at D83B12E5 blended fuel, engine load of 80% and rotational speed of 1900 rpm. However, the specific fuel consumption, with a minimum at the pure diesel, full load and engine rotational speed of 2453 rpm, increased with increment biodiesel and/or ethanol percentage in the fuel blends.

Ethanol as an electrolyte additive for alkaline zinc-air flow batteries

Zinc-air flow batteries exhibit high energy density and offer several appealing advantages. However, their low efficiency of zinc utilization resulted from passivation and corrosion of the zinc anodes has limited their broad application. In this work, ethanol, which is considered as an environmentally friendly solvent, is examined as an electrolyte additive to potassium hydroxide (KOH) aqueous electrolyte to improve electrochemical performance of the batteries. Besides, the effects of adding different percentages of ethanol (0–50% v/v) to 8 M KOH aqueous electrolyte were investigated and discussed. Cyclic voltammograms revealed that the presence of 5–10% v/v ethanol is attributed to the enhancement of zinc dissolution and the hindrance of zinc anode passivation. Also, potentiodynamic polarization and electrochemical impedance spectroscopy confirmed that adding 5–10% v/v ethanol could effectively suppress the formation of passivating layers on the active surface of the zinc anodes. Though the addition of ethanol increased solution resistance and hence slightly decreased the discharge potential of the batteries, a significant enhancement of discharge capacity and energy density could be sought. Also, galvanostatic discharge results indicated that the battery using 10% v/v ethanol electrolyte exhibited the highest electrochemical performance with 30% increase in discharge capacity and 16% increase in specific energy over that of KOH electrolyte without ethanol.

Study of the chromatographic parameters of ultra-high-performance supercritical fluid chromatography and method development using a design of experiments approach for the quantification of pesticides in lettuce.

We examine the potential of ultra-high-performance supercritical fluid chromatography for multiresidue quantification of ten pesticides commonly applied to lettuce and compares it to ultra-high-performance liquid chromatography. Initially, a thorough study of the stationary and mobile phase composition and injection solvent was carried out. In a second step, a chemometric approach based on design of experiments was used to simultaneously study the influence of temperature, pressure, and percentage of ethanol on the retention, resolution and symmetry of the peaks. Using this approach, it was possible to obtain the Design Space, a robust region where complete separation of the analytes was achieved, with acceptable peak shape. Both methods were validated according to the figures of merit: selectivity, linearity, quantification limit, accuracy (in terms of recovery), and precision (repeatability and intermediate precision) and used to quantify the pesticides in lettuce samples. Comparing both techniques, it was concluded that the limits of quantification, accuracy, and precision were similar. However, in supercritical fluid chromatography, a reduced volume of organic solvent was used, the method was faster and generated lower amounts of residues.

Purification and Lignocellulolytic Potential of Cellulase from Newly Isolated Acinetobacter indicus KTCV2 Strain

A novel approach of cellulase-based co-culture producing bioethanol using low-cost nutrient medium has been employed for the study, and docking strategies provided the information of cellobiose and cellotetraose inhibitors during cellulase production. The Acinetobacter species was isolated from termite gut and confirmed in 16s rDNA analysis. SDS-PAGE reveals the molecular weight of purified cellulose was 45 kDa. Cellulose activity was achieved maximum of 1.2 IU/mL at 48th hour of incubation, and about 80% of polysaccharides were converted into simple sugars. The enzyme activity was optimum at different physiological conditions like temperature at 37 °C, pH 7.0 and with 4% concentration of banana peduncle extract powder. Upon reaching maximum cellulolytic activity of 0.594 IU/mL, the percentage of ethanol produced from cellulosic hydrolysate using S. cerevisiae reached maximum ethanol (18.3 g/L) during 48 h of incubation. Cellulase produced by Acinetobacter indicus KTCV2 strain exhibits a short incubation period (48 h) and produces cellulase in broader pH and temperature ranges. Banana peduncle offers a cheapest raw material composed of cellulosic biomass in agricultural practices, which served as a good substrate for the production of ethanol.

Effect of Physical Properties on Conductivity Measurements of Acetyl acetonylbinylidine 4-aminosalycilic Acid in Different Percentages of Ethanol/ Water Mixture

Effect of Physical Properties on Conductivity Measurements of Acetyl acetonylbinylidine 4-aminosalycilic Acid in Different Percentages of Ethanol/ Water Mixture

Performance and Emission Characteristics of a Diesel Engine Fueled by Biodiesel-Ethanol-Diesel Fuel Blends

Abstract: There is growing interest to study the effect of blending various oxygenated additives with diesel or biodiesel fuel on engine performance and emission characteristics. This study aims to analyze the performance and exhaust emission of a four-stroke, four-cylinder diesel engine fueled with biodiesel-ethanol-diesel. Biodiesel was first produced from crude Jatropha oil, and then it was blended with ethanol and fossil diesel in different blend ratios (B10E10D80, B12.5E12.5D75, B15E15D70, B20E20D60 and B25E25D50). The engine performance and emission characteristics were studied at engine speeds ranging from 1200 to 2000 rpm. The results show that the brake specific fuel consumption increases while the brake power decreases as the percentage of biodiesel and ethanol increases in the blend. The exhaust emission analysis shows a reduction in CO2 emission and increase in NOx emission when the biodiesel -to- ethanol ratio increases in the blends, when compared with diesel as a reference fuel.

Performance and Emission Characteristics of a Diesel Engine Fueled by Biodiesel-Ethanol-Diesel Fuel Blends

There is growing interest to study the effect of blending various oxygenated additives with diesel or biodiesel fuel on engine performance and emission characteristics. This study aims to analyze the performance and exhaust emission of a four-stroke, four-cylinder diesel engine fueled with biodiesel-ethanol-diesel. Biodiesel was first produced from crude Jatropha oil, and then it was blended with ethanol and fossil diesel in different blend ratios (B10E10D80, B12.5E12.5D75, B15E15D70, B20E20D60 and B25E25D50). The engine performance and emission characteristics were studied at engine speeds ranging from 1200 to 2000 rpm. The results show that the brake specific fuel consumption increases while the brake power decreases as the percentage of biodiesel and ethanol increases in the blend. The exhaust emission analysis shows a reduction in CO2 emission and increase in NOx emission when the biodiesel -to-ethanol ratio increases in the blends, when compared with diesel as a reference fuel.

Effects of moderate, voluntary ethanol consumption on the rat and human gut microbiome.

Many alcohol-induced health complications are directly attributable to the toxicity of alcohol or its metabolites, but another potential health impact of alcohol may be on the microbial communities of the human gut. Clear distinctions between healthy and diseased-state gut microbiota have been observed in subjects with metabolic diseases, and recent studies suggest that chronic alcoholism is linked to gut microbiome dysbiosis. Here, we investigated the effects of moderate levels of alcohol consumption on the gut microbiome in both rats and humans. The gut microbiota of rats voluntarily consuming a 20 percent ethanol solution, on alternate days, were compared with a non-exposed control group to identify differential taxonomic and functional profiles. Gut microbial diversity profiles were determined using culture-independent amplification, next-generation sequencing and bioinformatic analysis of bacterial 16S ribosomal RNA gene sequence libraries. Our results showed that, compared with controls, ethanol-consuming rats experienced a significant decline in the biodiversity of their gut microbiomes, a state generally associated with dysbiosis. We also observed significant shifts in the overall diversity of the gut microbial communities and a dramatic change in the relative abundance of particular microbes, such as the Lactobacilli. We also compared our results to human fecal microbiome data collected as part of the citizen science American Gut Project. In contrast to the rat data, human drinkers had significantly higher gut microbial biodiversity than non-drinkers. However, we also observed that microbes that differed among the human subjects displayed similar trends in the rat model, including bacteria implicated in metabolic disease.

Bioethanol production using vegetable peels medium and the effective role of cellulolytic bacterial (Bacillus subtilis) pre-treatment.

Background: The requirement of an alternative clean energy source is increasing with the elevating energy demand of modern age. Bioethanol is considered as an excellent candidate to satiate this demand. Methods: Yeast isolates were used for the production of bioethanol using cellulosic vegetable wastes as substrate. Efficient bioconversion of lignocellulosic biomass into ethanol was achieved by the action of cellulolytic bacteria ( Bacillus subtilis). After proper isolation, identification and characterization of stress tolerances (thermo-, ethanol-, pH-, osmo- & sugar tolerance), optimization of physiochemical parameters for ethanol production by the yeast isolates was assessed. Very inexpensive and easily available raw materials (vegetable peels) were used as fermentation media. Fermentation was optimized with respect to temperature, reducing sugar concentration and pH. Results: It was observed that temperatures of 30°C and pH 6.0 were optimum for fermentation with a maximum yield of ethanol. The results indicated an overall increase in yields upon the pretreatment of Bacillus subtilis; maximum ethanol percentages for isolate SC1 obtained after 48-hour incubation under pretreated substrate was 14.17% in contrast to untreated media which yielded 6.21% after the same period. Isolate with the highest ethanol production capability was identified as members of the ethanol-producing Saccharomyces species after stress tolerance studies and biochemical characterization using Analytical Profile Index (API) ® 20C AUX and nitrate broth test. Introduction of Bacillus subtilis increased the alcohol production rate from the fermentation of cellulosic materials. Conclusions: The study suggested that the kitchen waste can serve as a raw material in ethanol fermentation.

Bioethanol production using vegetable peels medium and the effective role of cellulolytic bacterial (Bacillus subtilis) pre-treatment

Background: The requirement of an alternative clean energy source is increasing with the elevating energy demand of modern age. Bioethanol is considered as an excellent candidate to satiate this demand. Methods: Yeast isolates were used for the production of bioethanol using cellulosic vegetable wastes as substrate. Efficient bioconversion of lignocellulosic biomass into ethanol was achieved by the action of cellulolytic bacteria ( Bacillus subtilis). After proper isolation, identification and characterization of stress tolerances (thermo-, ethanol-, pH-, osmo- & sugar tolerance), optimization of physiochemical parameters for ethanol production by the yeast isolates was assessed. Very inexpensive and easily available raw materials (vegetable peels) were used as fermentation media. Fermentation was optimized with respect to temperature, reducing sugar concentration and pH. Results: It was observed that temperatures of 30°C and pH 6.0 were optimum for fermentation with a maximum yield of ethanol. The results indicated an overall increase in yields upon the pretreatment of Bacillus subtilis; maximum ethanol percentages for isolate SC1 obtained after 48-hour incubation under pretreated substrate was 14.17% in contrast to untreated media which yielded 6.21% after the same period. Isolate with the highest ethanol production capability was identified as members of the ethanol-producing Saccharomyces species after stress tolerance studies and biochemical characterization using Analytical Profile Index (API) ® 20C AUX and nitrate broth test. Introduction of Bacillus subtilis increased the alcohol production rate from the fermentation of cellulosic materials. Conclusions: The study suggested that the kitchen waste can serve as a raw material in ethanol fermentation.

Extraction of the same novel homoglycan mixture from two different strains of Bifidobacterium animalis and three strains of Bifidobacterium breve.

Three strains of Bifidobacterium breve (JCM 7017, JCM 7019 and JCM 2258) and two strains of Bifidobacterium animalis subsp. lactis (AD011 and A1dOxR) were grown in broth cultures or on plates, and a standard exopolysaccharide extraction method was used in an attempt to recover exocellular polysaccharides. When the extracted materials were analysed by NMR it was clear that mixtures of polysaccharides were being isolated including exopolysaccharides (EPS) cell wall polysaccharides and intracellular polysaccharides. Treatment of the cell biomass from the B. breve strains, or the B. animalis subsp. lactis AD011 strain, with aqueous sodium hydroxide provided a very similar mixture of polysaccharides but without the EPS. The different polysaccharides were partially fractionated by selective precipitation from an aqueous solution upon the addition of increasing percentages of ethanol. The polysaccharides extracted from B. breve JCM 7017 grown in HBM media supplemented with glucose (or isotopically labelled D-glucose-1-13C) were characterised using 1D and 2D-NMR spectroscopy. Addition of one volume of ethanol generated a medium molecular weight glycogen (Mw=1×105 Da, yield 200 mg/l). The addition of two volumes of ethanol precipitated an intimate mixture of a low molecular weight β-(1→6)-glucan and a low molecular weight β-(1→6)-galactofuranan which could not be separated (combined yield 46 mg/l). When labelled D-glucose-1-13C was used as a carbon supplement, the label was incorporated into >95% of the anomeric carbons of each polysaccharide confirming they were being synthesised in situ. Similar 1H NMR profiles were obtained for polysaccharides recovered from the cells of B. animalis subsp. lactis AD011and A1dOxR (in combination with an EPS), B. breve JCM 7017, B. breve JCM 7019, B. breve JCM 2258 and from an EPS (-ve) mutant of B. breve 7017 (a non-EPS producer).

Systematic study on the extraction of antioxidants from pinhão (araucaria angustifolia (bertol.) Kuntze) coat

Food by-products containing bioactive substances have attracted attention due to the possibility of adding values to residues of the food industry. In this work, the extraction of phenolic compounds from pinhão seed coats (Araucaria angustifolia (Bertol.) Kuntze) using a central composite rotatable design was applied to obtain prediction models for the extract volume yield, total phenolic content, total phenolic acids and total flavonoids. Principal component analysis and hierarchical cluster analysis were implemented showing an evident poor effect of the temperature on phenolic compounds extraction, which is in accordance with the prediction model obtained by the experimental design for total phenolic acids. Volume yield presented a high positive correlation with extraction temperature, followed by solvent composition. Scanning electron microscopy showed that higher temperatures and lower ethanol percentages resulted in highly defibrillated pinhão coats that retained more extract after the extraction process, leading to lower volume yield percentages.