Percentage Of Unsaturated Fatty Acids Research Articles

PHYTOCHEMICAL COMPOUNDS AND ANTIOXIDANT ACTIVITY OF WATER CHESTNUT (Eleocharis dulcis) AND GIANT MOLESTA (Salvinia molesta) EXTRACT

The purpose of this research was to observe of phytochemical content and antioxidant activity of chinese water chesnut (Eleocharis dulcis) and giant molesta (Salvinia molesta) extracts with three solvents of different levels of polarity. This study used an experimental laboratory method and data analysis was carried out descriptively. Some of the steps carried out include the stages of sampling, sample extraction, calculation of extract extracts, quantitative phytochemical analysis (flavonoid content, phenol content, phenolic content, and tannin content), and test of antioxidant activity (DPPH, ABTS and Reducing ability. The results showed that extracts of extract using ethanol in water chesnut and giant molesta had the highest values of 2.01% and 2.26%. Quantitative phytochemical tests on water chesnut and giant molesta extract showed that ethanol solvents at flavonoid levels had the highest values of 288.75 ppm and 267 ppm. The results of antioxidant activity with the water chesnut and giant molesta extracts had very weak IC50 values in n-hexan solvents valued at 1977.22 ppm and 3211.2 ppm. Water chesnut of extract had the highest ABTS value in ethyl acetate solvents at a concentration of 1000 ppm at 85.253% and in giant molesta extract at a concentration of 1000 ppm using ethanol solvent had the highest value of 76.665%. Ethanol extract in water chesnut extract and giant molesta had the highest reducing ability power with a mean absorbance of 0.477 and 0.520. The best solvent for extracting water chesnut and giant molesta were ethanol.

Effects of drying methods on compositional characterization and functional characteristics of Blighia sapida aril oil

The composition of Blighia sapida fruits is reported to differ based on its origin, variety, and preservation technique. In this study, the effect of drying method on the composition as well as the antioxidant activity of oven-dried and sun-dried B. sapida aril flour and oil were examined using standard procedures. Sun-dried B. sapida aril flour had significantly higher protein and fat content compared to the oven-dried flour. In addition, sun-dried B. sapida aril flour contained a more diverse phytochemical profile compared to the oven-dried flour. However, oven-dried B. sapida aril oil (ODAO) showed a higher percentage of unsaturated fatty acids compared to oil from the sun-dried B. sapida flour (SDAO). On the other hand, SDAO showed significantly higher DPPH scavenging and nitric oxide inhibitory activities compared to ODAO. Based on these findings, sun-drying is recommended for both nutritional purposes and other health-promoting usage such as antioxidant, over oven-drying.

Molecular characterization of blue-green algae (Anabaena constricta) and comparative studies of biodiesel production from other species

Biodiesel can substitute fossil fuels adequately and thus, considered ‘future fuel’. Thus, identifying a promising algal strain for efficient biodiesel production is a very important endeavor. In the present investigation, the blue green algae, Anabaena constricta could be grown aseptically attaining exponential phase, 3 days post inoculation, responds to phototropism and amenable to both autotrophic and heterotrophic mode. Phylogenetic analysis reveal that Anabaena constricta grouped separately from other algal strains used in the study, making it unique in nature. Further, comparative studies of selected algal and blue green algal strains based on the oil percentage and fatty acid profile indicate potential biodiesel properties. The total lipid content of Anabaena constricta is estimated to 14% of the dry weight, and fatty acids profiling indicate presence of 11 fatty acid methyl esters (FAMEs), principally palmitic acid (16:0), oleic acids (18:1), linoleic acid (18:2), palmitoleic acid (16:1). The algal species, Hydrodictyon registered highest percentage of unsaturated fatty acids (59.5%), while highest content of saturated fatty acid was found in Anabaena constricta (51.5%). Cetane Number of seven algal and blue green algal biodiesel varied from 54.77 to 58.2 and Saponification Value varied from 205.6 to 211.39. PROMETHEE & GAIA analyses indicate both the Rhizoclonium and Tolypothrix species outranked while Hydrodictyon and Anabaena constricta are the least suitable species in seven algal and blue-green algal species studied for biodiesel production. Well-characterized strains offer promise for biodiesel production at a cost-effective level. The present study focus on the renewable fuel, which is useful for reducing the carbon footprint with potential to impact self-sustainability in fuel sector, without modification in current infrastructure.

Phytochemical investigation using GC/MS analysis and evaluation of antimicrobial and cytotoxic activities of the lipoidal matter of leaves of Sophora secundiflora and Sophora tomentosa

This study aims at investigation of the phytochemical composition, antimicrobial and cytotoxic activities of the lipoidal matter of leaves of S. secundiflora (Ortega) and S. tomentosa L.. The saponifiable and unsaponifiable matter of S. secundiflora and S. tomentosa leaves were assessed using GC/MS analysis. Where, saponification of lipoidal matter of S. secundiflora and S. tomentosa leaves yielded 31.55%, 87.74% for unsaponifiable matter and 19.66%, 38.70% for fatty acids methyl esters of both species, respectively. The dominant compounds in the unsaponifiable matter of S. secundiflora were β-amyrin acetate 55.20% and α-amyrin 9.73%. Whereas n-nonacosane 43.80% and 2-methyltriacontane 11.94% were the main components in S. tomentosa. In the saponifiable fraction, the content of saturated fatty acids identified in S. tomentosa 58.37% is higher than S. secundiflora 29.0%, while the percentage of unsaturated fatty acids identified in S. secundiflora 62.67% is higher than S. tomentosa 34.51%. Methyl linolenate 36.62% and methyl palmitate 40.02% are the major compounds in S. secundiflora and S. tomentosa, respectively. The lipoidal matters were evaluated in vitro for cytotoxic activity towards HCT-116 carcinoma cell line using the MTT assay with IC50 value of 97.00 and 38.76 μg/mL for S. secundiflora and S. tomentosa, respectively. Using the technique of agar well diffusion, the lipoidal matter of S. secundiflora and S. tomentosa displayed moderate antimicrobial activity at conc. of 50 mg/mL.

Analysis of vegetable oil mixture combustion in a conventional 50 KW thermal energy installation

This work presents the combustion results of a vegetable oils blend in a low-pressure auxiliary air fluid pulverisation burner (o emulsion burner). This blend was prepared from soy and sunflower refined vegetable oils (54.4%) and sunflower and rapeseed crude vegetable oils (45.4%). The influence of varying the fuel flow rate, the secondary airflow rate as well as the fuel preheating temperature on combustion, performance and pollutant emissions was studied. Additionally, a comparison between the combustion results obtained from this mixture of vegetable oils and those previously obtained in combustion for pure vegetable oils was also carried out. For all of the conditions tested, the vegetable oil mixture combustion performance exceeded 77% and NOx emissions were extremely low (<60 ppm). In the established optimal operating conditions: un-preheated vegetable oil and Amid airflow rate, CO emissions were virtually negligible (5–17 ppm), regardless of the fuel flow used. The pollutant emissions and the combustion performance of the vegetable oils blend are comparable to those obtained in the combustion of refined soybean, sunflower and rapeseed oils, all of them with a high percentage of unsaturated fatty acids (mainly oleic and linoleic).

Seasonal Variation of Chemical Composition, Fatty Acid Profile, and Sensory Properties of a Mountain Pecorino Cheese.

This study aims to assess the compositional traits and sensory characteristics of a traditional pecorino cheese associated with management and feeding system seasonality. The study was carried out on two mountain dairy farms using an outdoor, pasture-based system from April to October (OutS), and an indoor system (InS) during the rest of the year. Outdoor-produced milk had higher fat content and a tendency for protein and somatic cell count to be higher. The OutS cheeses showed higher dry matter and fat content, higher percentages of unsaturated fatty acids, C18:3, cis-9, trans-11 conjugated linoleic acid, and trans-11 C18:1, and lower percentages of C14:0 and C16:0. These modifications in fatty acid composition determined the reduction of the atherogenic index. The OutS cheeses also displayed higher intensity of almost all sensory attributes, including odor, flavor, taste, and texture descriptors. The outdoor system partly reduced the liking of consumers for pecorino. However, changes in the productive process leading to an increment in the water content and softness of the cheeses (i.e., controlled humidity and temperature during ripening) may increase the overall liking of pasture-based products, thus promoting the consumption of healthier foods.

Chemical foot print of precomposted dung material of Elephus maximus and Bos taurus through GC–MS profile

The growth and reproductive potential of composting earthworm is influenced by the chemical composition of initial feed mixture. Thus the GC-MS technique is used to determine the biochemical foot prints of the vermibed substrate before composting process. The present study conducted to analyze the chemical composition of precomposted animal dung material of Elephus maximus and Bos taurus by GC-MS analysis. The dung materials were precomposted up to 14 days and used for the analysis. The GC-MS spectrogram of Elephus maximus and Bos taurus showed 20 and 15 different compounds respectively. Predominant levels of esters were observed in elephant dung, which were dominated by the esterified polyunsaturated fatty acids with maximum peak area for elaidinsaeure methyl ester (26.6%). In cow dung the maximum peak area was obtained for 1-Hydroxy-1-methyl) −2-Methyl tetrahydro −2 furanyl] − 4, 4, 14-Trimethylandrost- 9 (11) – ene −3, 6, 16 (17%) and alcohol were the dominant functional group followed by carboxylic acids and esters. Thus the results indicated that the presence of higher percentage of esterified poly unsaturated fatty acids in elephant dung reflects its higher quality suitable for vermicomposting, since the dietary unsaturated fatty acids have the positive impacts on the reproduction of earthworm by enhancing the functionality in sperm production.

RESPONSE SOME QUALITY CHARACTERISTICS OF SUNFLOWER (Helianthus annuus L.) VARIETY TO ZINC SPRAYING

A field experiment was carried out in the Spring and Autumn seasons of 2018 in Al-Subaihat village (latitude 33° 21- N and longitude 43° 46- E) Fallujah city - Al-Anbar governorate in silty-loam soil. To study the effect of two factors which were foliar zinc application different concentration of zinc 0, 0.5, 1.0 and 1.5 kg Zn h1 in the form of aqueous zinc sulfate ZnSO4.7H2O (46% Zn) on the Qualitative Characteristics of three varieties of sunflower (Tarsan8018, Esahaqi 1 and Aqmar). The layout of experiment RCBD ,arrangemental in Factorial experimental units distributed (36 experimental units) with three replicates. The results showed significant different between cultivars in most of the studied traits. Aqmar gave the highest oil content of 54.14 and 51.40% for the both spring and autumn respectively. For the autumn season, Tarsan1018 cv. recorded highest mean percentage of unsaturated fatty acids in seeds about 91.73% for spring season, While Isahaqi 1 exceeded the highest mean of 90.91% for autumn season. Isahaki 1 plants recorded the highest average carbohydrate percentage of 14.70 and 14.97% Zinc spraying resulted in a significant increase in the seed oil percentage. The concentration of 1.5 kg Zn h-1 achieved the highest mean ( 51.56% ) in spring season , while concentration of 1.0 kg Zn E-1 achieved the highest mean capacity in the autumn season (48.79%) Concentration 0.5 kg Zn h-1 are highest percentage of unsaturated fatty acids was 91.01%. In the autumn season, while the concentration of 1.0 kg Zn h-1 achieved the highest average percentage of unsaturated acids in seeds was 90.56%, also the concentration of 1.0 kg Zn h-1 gave the highest percentage of oleic acid in oil was 68.48% and 27.92% for the two season respectively.

Performance evaluation of different harvesting methods and cultivation media on the harvesting efficiency of microalga and their fatty acids profile

Harvesting techniques directly affect the quality and quantity of microalgal biomass as well as the total cost of final products. In this study, the harvesting efficiency of freshwater microalga, Scenedesmus quadricauda, from synthetic medium (Bold's Basal Medium, BBM) and real wastewater (dairy wastewater, DWW) was studied using ferric chloride (FeCl3). For this purpose, the effects of three variables namely solution pHs (3, 7, and 11), coagulant concentrations (100, 400, and 700 mg/L), and reaction times (5, 10 and, 15 min) on the harvesting efficiency of microalgae were evaluated. The highest harvesting efficiencies from BBM and DWW media were achieved as 95.75% (pH 11, 700 mg/L coagulant concentration, and 10 min reaction time) and 87.11% (pH 7, 700 mg/L coagulant concentration, and 15 min reaction time). The harvested microalgal biomasses were subsequently used for the lipid extraction. The analysis of lipids profile showed that the cultivation media (BBM and DWW), harvesting methods (coagulation and centrifugation), and biomass storage time (one day and two months) significantly affected the percentages of saturated fatty acids (SFAs) and poly unsaturated fatty acids (PUFAs) in microalgal lipids. The findings of this study revealed that the harvesting methods and cultivation media are two major factors that remarkably influenced the harvesting efficiency of microalga and the fatty acids of microalgal lipids as well.

Effect of Various Bicarbonate Supplements on Biodiesel Production and Valuable Biochemical Components of the Marine Eustigmatophyceae Nannochloropsis oculata (Droop)

Microbial breeding media must be cost-effective, enable high growth, meet exact requirements and be readily available. The effect of different levels of sodium bicarbonate (SB) [25, 50, 75 and 100%] in the growth medium on the biochemical constituents (protein, carbohydrates, lipids, fatty acids, and amino acids) of the Nannochloropsis oculata was assessed compared to the F/2 Guillard standard medium. The obtained results revealed that the chemical constituents of N. oculata were influenced by the used level of sodium bicarbonate. The highest total protein, carbohydrate contents, and the maximum percentage of essential amino acids (EAA) (59.68 %) were obtained by using the B1 medium (25% SB) as compared to the control (100% F/2). The highest total lipid content was achieved by using the B4 medium (100% SB) producing (20.09 %). In accordance, the highest total saturated fatty acids percentage (TSFA) of N. oculata was recorded by B4 medium. However, the highest total unsaturated fatty acids percentage (USFA) was exhibited by the B1 medium. The EN 14214 and ASTM D-6751 analyzes of the production of biodiesel have shown that the produced biodiesel by B4 medium (100% SB) and the control media of high quality. In conclusion, the addition of sodium bicarbonate is an excellent policy to increase chemical composition and lipid accumulation. The present study recommended taming results foreither biodiesel production or aquaculture feeding by using proposed B4 medium as a lipid promoter or B1 medium as a protein promoter.

Comparison of simple and rapid cell wall disruption methods for improving lipid extraction from yeast cells

The present study examined the effect of six disruption methods of the cell wall (acid hydrolysis, ultrasonication, osmotic shock, pasteurization, homogenization with zirconia balls, and freezing/defrosting) on the efficiency of lipid extraction from yeast cells and the composition of fatty acids. Acid hydrolysis and sonication led to a significant increase in lipid extraction from Cyberlindnera jadinii ATCC 9950 and Rhodotorula glutinis LOCKR13 yeast cells. The amount of lipids extracted in these conditions increased for C. jadinii from 12.46 (biomass not subjected to any pretreatment) to 20.37 and 19.53 g/100 gd.w. after the application of acid hydrolysis and sonication, respectively, and for R. glutinis strain from 13.95 to 21.20 and 17.22 g/100 gd.w., respectively, for the same methods. Initial sonication of biomass led to a significant reduction in the percentage of unsaturated fatty acids. The largest differences in fatty acid composition were found for the sample homogenized with zirconium balls. This process resulted in the degradation of both oleic acid and linolenic acid. The obtained results revealed that the method that significantly increases lipid extraction and does not change the composition of fatty acids is acid hydrolysis with hydrochloric acid. In addition, it is easy, cheap, does not require specialized equipment, and therefore can be implemented in any laboratory.

Molecular identification, biomass, and biochemical composition of the marine chlorophyte Chlorella sp. MF1 isolated from Suez Bay

BackgroundAn Egyptian indigenous unicellular green microalga was isolated from the coastal water of Suez Bay (N 29.92°, E 32.473°), Red Sea, Egypt. The molecular analysis based on 18S rRNA sequence showed that the gene sequence for this strain was highly similar (100% identity and 98% query cover) to different Chlorella strains isolated from different habitats. ResultsThe observed morphological characters together with the molecular phylogeny assigned the isolated microalga as Chlorella sp. MF1 with accession number KX228798. This isolated strain was cultivated for estimation of its growth and biochemical composition. The mean specific growth rate (μ) was 0.273 day−1. Both the biomass productivity and the cellular lipid content increased by increasing salinity of the growth medium, recording a maximum of 6.53 gDW l−1 and 20.17%, respectively, at salinity 40.4. Fourteen fatty acids were identified. The total saturated fatty acid percentage was 54.73% with stearic (C18:0), arachidic (C20:0), and palmitic acids (C16:0) as major components, while the total unsaturated fatty acid percentage was 45.27% with linoleic acid (C18:2c) and oleic acid (C18:1) as majors. ConclusionThis algal strain proved to be a potential newly introduced microalga as one of the most proper options available for microalgae-based biodiesel production. The proximate analysis showed the protein content at 39.85% and carbohydrate at 23.7%, indicating its accessibility to various purposes.

The Study of the Response of Fat Metabolism to Long-Term Energy Stress Based on Serum, Fatty Acid and Transcriptome Profiles in Yaks.

Simple SummaryThe serum, fatty acid and transcriptome profiles in the subcutaneous fat of yaks were measured to explore the effect of long-term energy stress (ES) on fat metabolism during the cold season. The study indicated that under long-term ES during the cold season, the amount of fat in yaks was less, and fat mobilization was one of the main ways by which energy was obtained in yaks. Yaks regulated fat metabolism in subcutaneous fat primarily through adenosine 5′-monophosphate-activated protein kinase (AMPK) signaling. Glucose (GLU) intake, fat catabolism, fatty acid synthesis and fatty acid oxidation in the subcutaneous fat of yaks were all inhibited, which resulted in the fat mobilization of yaks slowing as much as possible under long-term ES. In addition, the energy expenditures in fat cells were inhibited by regulating phosphatidylinositol 3’ -kinase (PI3K)-serine/threonine-protein kinase (Akt) andmammalian target of rapamycin (mTOR) signaling, and the limited energy obtained from GLU and fat was consumed by muscle and organs as much as possible. These factors led to an energy balance in yaks under long-term ES. The fat stored in yaks can be expended for as long as possible, and yaks can survive for as long as necessary under long-term ES.Long-term energy stress (ES) during the cold season is a serious problem for the breeding of yaks. In this paper, the response of fat metabolism in yaks to long-term ES during the cold season was studied. Gas chromatography (GC) analysis showed that the percentage of saturated fatty acids (SFAs) in the subcutaneous fat of the yaks in the ES group was 42.7%, which was less than the 56.6% in the CO group (p < 0.01) and the percentage of polyunsaturated unsaturated fatty acids (PUFAs) in the subcutaneous fat of the yaks in the ES group was 38.3%, which was more than the 26.0% in the CO group (p < 0.01). The serum analysis showed that fatty acid oxidation in yaks was increased under long-term ES. In the subcutaneous fat of yaks under long-term ES, the gene expression levels of glycerol-3-phosphate acyltransferase 4 (GPAT4), hormone-sensitive lipase (HSL), patatin-like phospholipase domain-containing protein 2 (PNPLA2), acyl-CoA dehydrogenase (ACAD), acyl-coenzyme A thioesterase 8 (ACOT8), facilitated glucose transporter (GLUT4), 3-oxoacyl-[acyl-carrier-protein] synthase (OXSM), oestradiol 17-beta-dehydrogenase 8 (HSD17B8) and malonate-Co-A ligase ACSF3 (ACSF3) were downregulated (q < 0.05), whereas the gene expression levels of aquaporin-7 (AQP7), long-chain-fatty-acid-CoA ligase (ACSL), elongation of very long chain fatty acids protein (ELOVL) and fatty acid desaturase 1 (FADS1) were upregulated (q < 0.05), indicating the inhibition of fat catabolism, fat anabolism, fatty acid oxidation, glucose (GLU) intake and SFA synthesis and the promotion of glycerinum (GLY) transportation and PUFA synthesis. Additional findings showed that the gene expression levels of leptin (LEP), adenosine 5′-monophosphate-activated protein kinase (AMPK) and phosphatidylinositol 3-kinase (PI3K) were upregulated (q < 0.05), whereas the gene expression levels of malonyl-CoA decarboxylase (MCD), sterol regulatory element-binding protein 1 (SREBF1), mammalian target of rapamycin (mTOR) and serine/threonine-protein kinase (AKT) were downregulated (q < 0.05), indicating that fat metabolism in the subcutaneous fat of yaks under ES was mainly regulated by AMPK signaling and mTOR and PI3K-AKT signaling were also involved. Energy consumption was inhibited in the subcutaneous fat itself. This study can provide a theoretical basis for the healthy breeding and genetic breeding of yaks.

English

The fruit seeds of the Northern Amazon Region were evaluated bromatologically and nutritionally in terms of the level of macro- and micro-elements and fatty acids present in them. Bromatologically, the seeds of camu-camu (369.08 kcal 100 g-1) and birib&aacute; (364.78 kcal 100 g-1) have the highest energy value. In terms of the macroelements, the highest concentration was potassium (554.23 mg 100 g-1) seen in graviola, followed by magnesium (123.11 mg 100 g-1) in birib&aacute;. In terms of micronutrients, the highest concentrations are zinc (4.14 mg 100 g-1) and manganese (4.12 mg 100 g-1) in abiu seeds. The fatty acids composition of the seeds studied had a higher percentage of unsaturated fatty acids. Among the most saturated is palmitic acid with a concentration of 40.4% for tapereb&aacute; seeds while among the unsaturated is oleic acid with a concentration of 47.4% for bacupar&iacute; seeds. Key words: Oleic acid, bromatological, minerals, saturated fatty acids (SFA), unsaturated fatty acids (UFA). &nbsp

Enrichment diets of pigs with oil blends and its effects on performance, carcass characteristics and fatty acid profile

ABSTRACT The addition of different oil blends in the feed of finishing pigs was evaluated. Twenty-four castrated male finishing pigs were used in a randomized block design containing four treatments and six replicates. The treatments consisted of: Reference ration (RR) - 100% soybean oil feed; and the combination of the different oils: Blend1 - 50.0% soybean oil (SO), 25.0% flaxseed oil (FO), 12.5% olive oil (OO) and 12.5% canola oil (CO); Blend2 - 25.0% SO, 50.0% FO, 12.5% OO and 12.5% CO; and Blend3 - 25.0% SO, 12.5% FO, 12.5% OO and 50.0% CO. The performance, quantitative and qualitative carcass parameters, fatty acids profile and economic feasibility of the diets were evaluated. The use of blends in the diets did not influence the performance or carcass quality, but increased marbling and carcass yield. The fatty acid profile of the loin presented greater amounts of stearic acid in Blend3 and higher percentage of unsaturated fatty acids in animals fed with Blend1. The fatty tissue presented greater amounts of myristic acid in Blend1 and oleic acid in Blend3. The reference ration was the most economic. The Blends did not affect performance or carcass characteristics and improved the fatty acid profile.

THE EFFECT OF N-STEAROYLETHANOLAMINE ON THE ADIPOCYTE FATTY ACID COMPOSITION OF DIFFERENT AGE RATS WITH OBESITY-INDUCED INSULIN RESISTANCE

Chronic hypernutrition and high fat diet (HFD), rich in saturated fatty acids leads to molecular changes in insulin sensitive tissues and is followed by dyslipidemia. That is why the aim of our study was to investigate the fatty acid (FA) composition of phospholipids (PL), free fatty acids (FFA), triacylglycerol (TAG) and cholesterol esters (CE) of adipocytes in different age rats with HFD-induced insulin resistance (IR) and its changes under N-stearoylethanolamine (NSE) administration. The experimental model was induced on rats in age 10-month-old and 24-month-old by 6-month HFD and confirmed by the oral glucose tolerance test. NSE was administrated as water suspension per os in a dosage 50 mg/kg daily during 2 weeks. Adipocytes were isolated from abdominal fat using Type 1 Collagenase solution. Adipocytes lipid extract was separated on the fractions by thin-layer chromatography. The fatty acid composition of lipid fractions was analyzed by gas-liquid chromatography. Experimental data were processed statistically using Student’s t-test. It was demonstrated, that prolonged HFD induces IR and leads to changes in FA profile of adipocytes PL, TAG, CE and composition of FFA in rats from two age groups. The results we obtained shoved that the aging process affects the fatty acid composition of adipocytes. Particularly, there was a significant decrease in the amount of fatty acids in the fractions of phospholipids, triacylglycerols and cholesterol esters together with a decrease in the percentage of unsaturated fatty acids. It was also demonstrated, that HFD significantly alters the fatty acid composition of all investigated adipocytes lipid fractions of younger age group rats, while similar changes were much less manifested in older age group of animals. NSE administration had a positive effect on the normalization of the fatty acid composition of the studied lipid fractions of both age group rat adipocytes. This study demonstrated that prolonged HFD induces obesity, increases the risk of type 2 diabetes development and leads to changes in adipocytes FA profile in rats from two age groups. As far as NSE administration had a positive effect on normalization of FA composition of adipocytes, we can consider NSE as a prospective agent for the treatment of obesity-induced complications and correction of age-related dyslipidemia.

Metabolic engineering of Yarrowia lipolytica for heterologous oleanolic acid production

Oleanolic acid (OA) is a pentacyclic triterpenoid known for its biological properties such as antioxidation activity. Herein, we developed an efficient biosynthetic pathway for OA production in Yarrowia lipolytica. The engineered strain, YL-OA0, produced 16.3 mg/L of OA, and the production efficiency was further increased by 5.65-fold to 92.1 mg/L following overexpression of the key genes involved in the mevalonate (MVA) pathway. Transcriptomic and metabolomic analyses demonstrated the increase in the percentage of unsaturated fatty acids. The electron transfer of the strain YL-OA7 was restricted. Therefore, we fused the gene encoding cytochrome P450 (CYP716A12) to that encoding NADPH-P450 reductase; this resulted in an increase in the OA yield to 129.9 mg/L in the strain YL-fuOA1. The strain YL-fuOA1 could produce 540.7 mg/L OA in fed-batch fermentation in a 5 L fermenter. This study shows the feasibility of the production of high value triterpenoid compounds using Y. lipolytica as a platform.

In vitro gas production and rumen fermentation profile of fresh and ensiled genetically modified high–metabolizable energy ryegrass

We previously generated a high-metabolizable energy (HME) perennial ryegrass (Lolium perenne) by genetically modifying the plant to increase the leaf lipid content. Although substantial progress has been made toward characterizing physiological changes of HME ryegrass, very limited information exists for feeding value and its suitability for adoption into the pastoral system. In this study, independent HME ryegrass lines with a range of elevated leaf lipid concentrations were analyzed for changes in fatty acids and possible associated changes in the broader nutritional profile, including the gross energy, which was found to increase by 6.8%. Because ryegrass is often ensiled and fermentation in the rumen leads to biohydrogenation of fatty acids as well as enteric methane production, we sought to investigate these effects on HME ryegrass. This was achieved by performing mini-scale silos and using an automated gas measurement system to incubate the material in rumen fluid in vitro for 24 h. Our study included treatments comprising 3 independent HME ryegrass genotypes and wild-type control materials prepared fresh and as silage, employing in total 5 incubation studies, using rumen fluids collected from 4 nonlactating Jersey × Holstein cows. At intervals during the incubation, the production of gases, volatile fatty acids, and the degree of biohydrogenation were measured. Statistical data analysis indicated that differences in the nutritional compositions of the ensiled materials largely reflected those of their fresh counterparts. Incubation of both fresh and ensiled HME ryegrass in rumen fluid resulted in: (1) a greater percentage of valuable unsaturated fatty acids compared with the control; (2) a significant reduction of butyrate; and (3) a 10 to 15% decrease in the methane proportion of the total gas production. We conclude that ensiling could be a convenient option for preserving HME as a locally produced high-value supplementary feed; however, large-scale application needs to be investigated. In this paper we discuss the potential use of HME ryegrass to enhancing forage feeding value and the potential environmental benefits to the pastoral agriculture industry.

Composición de ácidos grasos de extractos etéreos de Bryophyllum pinnatum Lam., Ficus exasperata Vahl., Gossypium herbaceum Linn. y Hilleria latifolia (Lam.) H. Walt

Actualmente, existe un creciente interés en identificar fuentes alternativas de ácidos grasos debido a la creciente demanda actual de productos botánicos ricos en aceite en aplicaciones industriales. El objetivo principal de este trabajo fue identificar las composiciones de ácidos grasos de extractos de éter de hojas secas de Bryophyllum pinnatum Lam., Ficus exasperata Vahl., Gossypium herbaceum Linn. y Hilleria latifolia (Lam.) H. Walt. Los ácidos grasos fueron analizados por cromatografía de gases con detector de ionización de llama. Entre los extractos de éter evaluados, los mayores contenidos de ácidos grasos saturados se encontraron en H. latifolia (27,96%) con presencia principal de ácido esteárico en comparación con el contenido de los ácidos grasos saturados en B. pinnatum (0,53%), F. exasperata (0,04%) y G. herbaceum (0,47%). Igualmente, el resultado mostró que la H. latifolia contenía el mayor porcentaje de ácidos grasos insaturados con presencia predominante de ácido oleico con una cantidad de 41,04%. También se encontró que el ácido linoleico tiene el valor más alto en H. latifolia con una cantidad de 20,41%. Se encontró ácido esteárico, ácido oleico y ácido linoleico en todas las muestras. El extracto de H. latifolia contenía una mezcla saludable de diferentes tipos de ácidos grasos, lo que sugiere que es una fuente probable de ácidos grasos adecuados.

Impact of inbreeding on milk fatty acids of a Brazilian Holstein cattle

Context Reproductive technologies may either contribute to the increase of genetic gains or inbreeding levels in dairy cattle breeding programs and herds. In general, studies have evaluated the impacts of inbreeding on traditional traits, such as production and reproduction. However, studies on novel traits, such as milk fatty acids profile, are scarce in the literature. Aim We aimed to evaluate the impact of inbreeding levels on milk fatty acids profile measured in a Brazilian Holstein cattle population. Methods Monthly records of the percentages (%) of palmitic, stearic, oleic, total saturated, unsaturated, monounsaturated and polyunsaturated fatty acids in milk were collected from 2047 Holstein cows between May and December 2012. The pedigree file contained 7963 animals over seven generations. The inbreeding trend was evaluated by linear regression of inbreeding coefficients on generations. The inbreeding impacts on breeding and phenotypic values were evaluated by linear regression of breeding values and phenotypic values on generations respectively. Breeding values were obtained by a Bayesian approach based on an animal model that included the systematic effect of contemporary group, cubic effect of days in milk as a covariate, and the random direct additive genetic and animal permanent environmental effects. Key results The average inbreeding coefficient was 0.28% for all animals, and 1.39% for inbred animals. Inbreeding tended to increase 0.24% per generation (P-value &amp;lt;0.0001). The regression coefficients of phenotypic values on inbreeding were not significant for saturated, unsaturated, monounsaturated, palmitic, stearic and oleic fatty acids (P-values &amp;gt;0.18, 0.10, 0.16, 0.26, 0.51 and 0.09 respectively); whereas for polyunsaturated fatty acids, they were significant (P-value &amp;lt;0.04), with an increase of –0.000978% on phenotypic values for each 1% increase in inbreeding. The regression coefficients of breeding values on inbreeding were not significant for unsaturated, monounsaturated, polyunsaturated, stearic and oleic fatty acids (P-values &amp;gt;0.33, 0.78, 0.48, 0.32 and 0.07 respectively). However, they were significant for saturated and palmitic fatty acids (P-values &amp;lt;0.03 and 0.0001 respectively), presenting an increase of 0.000689% and 0.000283% respectively on breeding values for each 1% increase in inbreeding. Conclusions There was a significant increase in inbreeding coefficients over the studied generations; however, the impacts on both breeding and phenotypic values were small, when significant. Implications Our study may contribute to the understanding of how inbreeding levels may affect the fatty acids profile in a Brazilian Holstein cattle population, as well as encourage future research on this subject.